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The map label for this gene is ponA [H]

Identifier: 187735735

GI number: 187735735

Start: 1490161

End: 1491954

Strand: Direct

Name: ponA [H]

Synonym: Amuc_1241

Alternate gene names: 187735735

Gene position: 1490161-1491954 (Clockwise)

Preceding gene: 187735734

Following gene: 187735736

Centisome position: 55.93

GC content: 54.63

Gene sequence:

>1794_bases
ATGGAGAACAGGCGTAAAAGATGGTATGATCTGTTTGGATTGCTGCCTCGCAATCTGGGCCGTCTGTTGCTGTGGTTCAG
CCTCATTCCTATCGCTTTCATCATCGTTCTTCTCGTTTATGCATGGAGGGCGGACCAGTATGAGCTGGACGAGGTTTTGG
CGCCGTTGGAAAATTGCGCCGCATATGATCGCAACGGCCAGTGGATAGGCACCTTGACAGATCATGACCGGGTGTATGTA
GCGCGCAATGAACTGCCCGATAATCTGGTGAATGCTTTCGTTGCGCGGGAAGATGAAGACTTTTTCGAACATGGCGGCAT
TGTGTACTCTTCCATTATCCGCTCCATTTGCCGGAATTTGACTACATTGTCCTATGCCCAGGGTGCTTCCACGATTACGA
TGCAGCTGGCGCGGAATTGTTATGAGCTGGGAGGGAAGACCCTGGATCGCAAGGTTTTGGAAATGGCCGTGGCCCGCCGC
ATTGAGGGCAAGTATTCCAAGGATGAAATTCTGACGGCTTACCTGAACCGCATTTACTTTGGCCAGCAATGCTATGGGAT
TGCGCAGGCGGCGGATTTGTATTTCGGCAAAAAAGTGGCGGATCTGACACTGGCGGAGTGCGCTACCCTGGCCGGGCTGG
TGCGCGGACCTTCCATCTACAATCCCGTTTACAGCCCGGAGGCTGCTGCCAAGGTCCGCAATGCGACGCTGGAAAGGATG
TTTGAGTGTGAATTCATTACCCAGGAACAATTATGGCAGGCGCTCCGCGAACCGATGGCGGTGGCTGGTAAACGGGAAGC
TCGGCCCGGTTCTTATCCCGTTCTGGTCGTTTCCCGTGAGTTGGGAGACCTGGATTGCTGTGACGAGCAGGAGGGAACCT
CCAGCATTTTTGTAATGACCACGCTGGATTTGGAATTTCAGCGGATGGTTGAAGATGTAAGCGAGCCTATGCTGGTCGCT
CTGGAATCTTCTTCCGTGTGGGCAGGGTTGCCCAAAAGGGTGGACAACCAGTTGAATCGTTGTGTGCAAGCGGCCATATT
GTGTGTGGATTCCCGTAAAGGGGATCTGCTGGCCGTAACAGGGGGGCGTTCCGCCCAGGACGGTCTGGACCGATGGCAGT
CCAAGGTAATGCCCGGCGATCTGTTCACTCCCATTGTCAATTTATGCGCTGTCGACCAGCGTAGAACCGTTATTCGCAGC
AATCCGGAAGTCACTGGCCGCGGGGTAGGTTTCAATACAGTAATAGAAACGGCACAAAAGGCCGGTTACAAAGGGGAACT
TCCCCGTTCTTCGGACTTGTATACCGGCAGGTTTTCCGTGCCTCTTTCGCATGCCGTCAATGCCCTGTATCTCATCGGCA
ATGACGGGCTCAATGTGAGTATTTCCTCCGTCAGGCAGGTGGGTACCACCAAAAAGAACCTGGTGATGGTGAATGCCCCC
TCTCCGGAAGAAAGCCGCCGGGAAATCCTGCCTAGGGAATCCGCCCACCTGGTGGCTTCCCTTCCTCCCTTCCGCTACGA
CGAACGGACCAGGAAAACCTTCGTCAATGTCAGGCTTCCTGGAAATACGGGGCATTTTTCCGCCGTCATGGGGCGTTATT
TCACGGTTTTTGCCTGGGTGGGCTTTGATTCTCCGGAGGCCGCCGTCTATGAAAAAAAAGGGGTTTCCGCGGCGTTGGCA
AAAACCTGTTCCTCTCTGGCCGGGGAGGTGTATGACCGTGCGGAGGAAGGTCGCAGAAAAGCTGTCCGGGAACGCCGGGA
GCGGGAAAATGCTGCAGAACAGGGCTCCCAATGA

Upstream 100 bases:

>100_bases
ATACCGGGCCCGCCTGCAAAAGGAATTCCCGGATTGGAAACAGGATGGAGAGTCTTTGCCTCCGGACGCACGATAAGCGT
TTTAAAGATTGAAACCGGAA

Downstream 100 bases:

>100_bases
AGTTCAATTTTCCATTAGTAGAAGCATCATGAGACACTCACTTGAAATGACCACGGCCATCCAGGCCGCCAAATCCGCCG
GAGCTTTCCTGAAGAAGCAT

Product: glycosyl transferase family 51

Products: NA

Alternate protein names: PBP1; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MENRRKRWYDLFGLLPRNLGRLLLWFSLIPIAFIIVLLVYAWRADQYELDEVLAPLENCAAYDRNGQWIGTLTDHDRVYV
ARNELPDNLVNAFVAREDEDFFEHGGIVYSSIIRSICRNLTTLSYAQGASTITMQLARNCYELGGKTLDRKVLEMAVARR
IEGKYSKDEILTAYLNRIYFGQQCYGIAQAADLYFGKKVADLTLAECATLAGLVRGPSIYNPVYSPEAAAKVRNATLERM
FECEFITQEQLWQALREPMAVAGKREARPGSYPVLVVSRELGDLDCCDEQEGTSSIFVMTTLDLEFQRMVEDVSEPMLVA
LESSSVWAGLPKRVDNQLNRCVQAAILCVDSRKGDLLAVTGGRSAQDGLDRWQSKVMPGDLFTPIVNLCAVDQRRTVIRS
NPEVTGRGVGFNTVIETAQKAGYKGELPRSSDLYTGRFSVPLSHAVNALYLIGNDGLNVSISSVRQVGTTKKNLVMVNAP
SPEESRREILPRESAHLVASLPPFRYDERTRKTFVNVRLPGNTGHFSAVMGRYFTVFAWVGFDSPEAAVYEKKGVSAALA
KTCSSLAGEVYDRAEEGRRKAVRERRERENAAEQGSQ

Sequences:

>Translated_597_residues
MENRRKRWYDLFGLLPRNLGRLLLWFSLIPIAFIIVLLVYAWRADQYELDEVLAPLENCAAYDRNGQWIGTLTDHDRVYV
ARNELPDNLVNAFVAREDEDFFEHGGIVYSSIIRSICRNLTTLSYAQGASTITMQLARNCYELGGKTLDRKVLEMAVARR
IEGKYSKDEILTAYLNRIYFGQQCYGIAQAADLYFGKKVADLTLAECATLAGLVRGPSIYNPVYSPEAAAKVRNATLERM
FECEFITQEQLWQALREPMAVAGKREARPGSYPVLVVSRELGDLDCCDEQEGTSSIFVMTTLDLEFQRMVEDVSEPMLVA
LESSSVWAGLPKRVDNQLNRCVQAAILCVDSRKGDLLAVTGGRSAQDGLDRWQSKVMPGDLFTPIVNLCAVDQRRTVIRS
NPEVTGRGVGFNTVIETAQKAGYKGELPRSSDLYTGRFSVPLSHAVNALYLIGNDGLNVSISSVRQVGTTKKNLVMVNAP
SPEESRREILPRESAHLVASLPPFRYDERTRKTFVNVRLPGNTGHFSAVMGRYFTVFAWVGFDSPEAAVYEKKGVSAALA
KTCSSLAGEVYDRAEEGRRKAVRERRERENAAEQGSQ
>Mature_597_residues
MENRRKRWYDLFGLLPRNLGRLLLWFSLIPIAFIIVLLVYAWRADQYELDEVLAPLENCAAYDRNGQWIGTLTDHDRVYV
ARNELPDNLVNAFVAREDEDFFEHGGIVYSSIIRSICRNLTTLSYAQGASTITMQLARNCYELGGKTLDRKVLEMAVARR
IEGKYSKDEILTAYLNRIYFGQQCYGIAQAADLYFGKKVADLTLAECATLAGLVRGPSIYNPVYSPEAAAKVRNATLERM
FECEFITQEQLWQALREPMAVAGKREARPGSYPVLVVSRELGDLDCCDEQEGTSSIFVMTTLDLEFQRMVEDVSEPMLVA
LESSSVWAGLPKRVDNQLNRCVQAAILCVDSRKGDLLAVTGGRSAQDGLDRWQSKVMPGDLFTPIVNLCAVDQRRTVIRS
NPEVTGRGVGFNTVIETAQKAGYKGELPRSSDLYTGRFSVPLSHAVNALYLIGNDGLNVSISSVRQVGTTKKNLVMVNAP
SPEESRREILPRESAHLVASLPPFRYDERTRKTFVNVRLPGNTGHFSAVMGRYFTVFAWVGFDSPEAAVYEKKGVSAALA
KTCSSLAGEVYDRAEEGRRKAVRERRERENAAEQGSQ

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein. Note=Probably found all over the whole cell at low concentrations. Also localizes to the division site in vegetative cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 fibronectin type-III domain [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=196, Percent_Identity=43.8775510204082, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1786343, Length=333, Percent_Identity=30.9309309309309, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788867, Length=469, Percent_Identity=28.7846481876333, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1789601, Length=177, Percent_Identity=34.4632768361582, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR001264
- InterPro:   IPR013783
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00041 fn3; PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 66719; Mature: 66719

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENRRKRWYDLFGLLPRNLGRLLLWFSLIPIAFIIVLLVYAWRADQYELDEVLAPLENCA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AYDRNGQWIGTLTDHDRVYVARNELPDNLVNAFVAREDEDFFEHGGIVYSSIIRSICRNL
HCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHH
TTLSYAQGASTITMQLARNCYELGGKTLDRKVLEMAVARRIEGKYSKDEILTAYLNRIYF
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GQQCYGIAQAADLYFGKKVADLTLAECATLAGLVRGPSIYNPVYSPEAAAKVRNATLERM
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
FECEFITQEQLWQALREPMAVAGKREARPGSYPVLVVSRELGDLDCCDEQEGTSSIFVMT
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEE
TLDLEFQRMVEDVSEPMLVALESSSVWAGLPKRVDNQLNRCVQAAILCVDSRKGDLLAVT
EECHHHHHHHHHCCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEE
GGRSAQDGLDRWQSKVMPGDLFTPIVNLCAVDQRRTVIRSNPEVTGRGVGFNTVIETAQK
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHH
AGYKGELPRSSDLYTGRFSVPLSHAVNALYLIGNDGLNVSISSVRQVGTTKKNLVMVNAP
CCCCCCCCCCCCCCCCEECCCHHHCCCEEEEEECCCCCEEHHHHHHHCCCCCCEEEEECC
SPEESRREILPRESAHLVASLPPFRYDERTRKTFVNVRLPGNTGHFSAVMGRYFTVFAWV
CCCHHHHHCCCCCCCHHEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHEEE
GFDSPEAAVYEKKGVSAALAKTCSSLAGEVYDRAEEGRRKAVRERRERENAAEQGSQ
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MENRRKRWYDLFGLLPRNLGRLLLWFSLIPIAFIIVLLVYAWRADQYELDEVLAPLENCA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AYDRNGQWIGTLTDHDRVYVARNELPDNLVNAFVAREDEDFFEHGGIVYSSIIRSICRNL
HCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHH
TTLSYAQGASTITMQLARNCYELGGKTLDRKVLEMAVARRIEGKYSKDEILTAYLNRIYF
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GQQCYGIAQAADLYFGKKVADLTLAECATLAGLVRGPSIYNPVYSPEAAAKVRNATLERM
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
FECEFITQEQLWQALREPMAVAGKREARPGSYPVLVVSRELGDLDCCDEQEGTSSIFVMT
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEE
TLDLEFQRMVEDVSEPMLVALESSSVWAGLPKRVDNQLNRCVQAAILCVDSRKGDLLAVT
EECHHHHHHHHHCCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEEE
GGRSAQDGLDRWQSKVMPGDLFTPIVNLCAVDQRRTVIRSNPEVTGRGVGFNTVIETAQK
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHH
AGYKGELPRSSDLYTGRFSVPLSHAVNALYLIGNDGLNVSISSVRQVGTTKKNLVMVNAP
CCCCCCCCCCCCCCCCEECCCHHHCCCEEEEEECCCCCEEHHHHHHHCCCCCCEEEEECC
SPEESRREILPRESAHLVASLPPFRYDERTRKTFVNVRLPGNTGHFSAVMGRYFTVFAWV
CCCHHHHHCCCCCCCHHEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHEEE
GFDSPEAAVYEKKGVSAALAKTCSSLAGEVYDRAEEGRRKAVRERRERENAAEQGSQ
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7814321; 8760912; 9384377; 9721295; 10322023 [H]