Click here to switch to the map view.

The map label for this gene is parE [H]

Identifier: 187735688

GI number: 187735688

Start: 1429742

End: 1431583

Strand: Reverse

Name: parE [H]

Synonym: Amuc_1194

Alternate gene names: 187735688

Gene position: 1431583-1429742 (Counterclockwise)

Preceding gene: 187735692

Following gene: 187735687

Centisome position: 53.74

GC content: 56.79

Gene sequence:

>1842_bases
ATGGCCTACGACGAAAACAGCATCAAAACCCTGGATTGGAGGGAACACATCCGCATGCGCCCGGGGATGTACATCGGCAA
ACTGGGTGACGGAGCCTCCCCGGACGACGGCATTTACGTCCTTCTGAAAGAAGTCATCGACAACTCAATCGACGAATTCG
TGATGGGGTTCGGCAAAAAAATCACCATTGACGTGACTCCGGACGGCCTGTGCGAAGTGCGCGACTTCGGCCGCGGCATC
CCGCTGGGCAAACTGCTGGACTGCGCCGCAAAAATCAACACCGGGGCCAAATATGACAGCGACGCCTTCAAAAAATCCGT
AGGCCTCAATGGAGTGGGCATCAAGGCGGTCAACGCCCTGTCCGACTTCTTTGAAATCCAGGCCTATCGCGACGGGGAAA
CGCGTTCCTACCAGTTCTGCCGCGGAAAGGAAATCCCCAAGGGACGGAAGGACGGAGCCACGGAGGAACCCAACGGCACC
CGCACGGCTTTCCACGCGGACCCGGACATCTTTCCCGCGGCCACGCAATTCCGGCCGGAGTATATCGAAAAAATGTGCAG
ATACTACTCCTACCTCAACAAAGGGCTGGCCTTGCATTTCAACGGCCGCCGCTACATCTCCAAAAACGGGTTGCTGGACC
TGCTTGCGGAAGCGATGAGCGAGGCCCCCCTGTACCCCATCATCCATCTGGAAGGGCATGACATTGAAGTGGCCTTCACC
CACGGCGGCCAATACGGGGAGGAGCACTACTCCTTCGTCAACGGGCAGCACACCACCCAGGGAGGCACCCACCAGGCGGC
CTTCCGGGAAGCCATCGTCAAAACCCTGAGGGACTTCTTCAAGAAAAACTATGAGGCGGGGGACATCCGTTCCTCCCTCG
TAGCCGCCATCTCCATCAAGGTAAAGGAACCCGTCTTCGAATCCCAGACCAAAACCAAGCTGGGGTCCAACACCATCAGC
CCGGAAGGGGAAACCATCCGCACCTTTGTGGGCAACTTCATTGCCAGGGAGCTGGACAACTACCTGCACAAAAACCCGGA
AACGGCCAAAGCTCTGGAAGCAAAAATCAAGGATTCCGAACGGGACCGCAAGGAACTCTCCGGTATCCAGAAGCTGGCGC
GCGAAAACGCCCGCAAGGCCAAAATTCACAACAAGAACCTCCGGGACTGCCGGGTGCATTACAACTCCAAACACTCCCGC
GCCGGGGAAACCACCCTCTTCATTACGGAAGGCATCTCCGCCTCCGGCTCCATCACTACGGCGCGCGACGCGGAAACCCA
GGCCGTTTTCTCCCTGCGGGGCAAGCCGCTGAACTCCTTCGGCATGAGCCGTAAAGTCGTCTATGAAAATGAGGAATTCA
ACTTCCTCCAGCATGCCCTGAATATTGAAAACGGACTGGAGGAACTGCGCTATGACAAGGTAGTCATCGCCACGGACGCC
GATGACGACGGCATGCACATCCGCATCCTTTTGATGACATTCTTCATCCAGTTCTTTCCGGAATTGATCCGGGAGGGACA
CCTCTTCATCCTCCAGACCCCCCTCTTCCGCGTGCGCAACAAACAGCAAACCATTTACTGCTACTCGGACGCAGAGAGGG
AGGAAGCCATCAGCCTGCTGGGGAAAAACCCGGAAATTACCCGGTTCAAAGGCCTGGGAGAAATCTCCCCCCAGGAATTC
AAGGCTTTCATCGGGGAAGGGATGCGCCTGGACCGCGTCCGGCTGGAAGACTCCCACAAGGTAAAAGACCTGCTGTCCTT
CTACATGGGCAAAAACACGCGGGAAAGACAGGAATACATCCTGAACAACTTGCGTGTTGAACTGGACCCGGTCATGGACT
AG

Upstream 100 bases:

>100_bases
GGCCTCCGGGTCAAGCTCCATCGAACGCAGACGGACAGCCCCTCATTTTTTCTTCGCAAATTCCGGGGGCGCCGCTATAA
TAAGCGCGACCCCGTTGCAC

Downstream 100 bases:

>100_bases
AGTAACGGAGCGACGCTTATCTATGCTTACCATTCCCAAGGAAAAACTGCCGACTCACATCGCCTGCATCATGGACGGCA
ACGGCCGCTGGGCGAACAGC

Product: DNA topoisomerase IV subunit B

Products: Double stranded DNA [C]

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MAYDENSIKTLDWREHIRMRPGMYIGKLGDGASPDDGIYVLLKEVIDNSIDEFVMGFGKKITIDVTPDGLCEVRDFGRGI
PLGKLLDCAAKINTGAKYDSDAFKKSVGLNGVGIKAVNALSDFFEIQAYRDGETRSYQFCRGKEIPKGRKDGATEEPNGT
RTAFHADPDIFPAATQFRPEYIEKMCRYYSYLNKGLALHFNGRRYISKNGLLDLLAEAMSEAPLYPIIHLEGHDIEVAFT
HGGQYGEEHYSFVNGQHTTQGGTHQAAFREAIVKTLRDFFKKNYEAGDIRSSLVAAISIKVKEPVFESQTKTKLGSNTIS
PEGETIRTFVGNFIARELDNYLHKNPETAKALEAKIKDSERDRKELSGIQKLARENARKAKIHNKNLRDCRVHYNSKHSR
AGETTLFITEGISASGSITTARDAETQAVFSLRGKPLNSFGMSRKVVYENEEFNFLQHALNIENGLEELRYDKVVIATDA
DDDGMHIRILLMTFFIQFFPELIREGHLFILQTPLFRVRNKQQTIYCYSDAEREEAISLLGKNPEITRFKGLGEISPQEF
KAFIGEGMRLDRVRLEDSHKVKDLLSFYMGKNTRERQEYILNNLRVELDPVMD

Sequences:

>Translated_613_residues
MAYDENSIKTLDWREHIRMRPGMYIGKLGDGASPDDGIYVLLKEVIDNSIDEFVMGFGKKITIDVTPDGLCEVRDFGRGI
PLGKLLDCAAKINTGAKYDSDAFKKSVGLNGVGIKAVNALSDFFEIQAYRDGETRSYQFCRGKEIPKGRKDGATEEPNGT
RTAFHADPDIFPAATQFRPEYIEKMCRYYSYLNKGLALHFNGRRYISKNGLLDLLAEAMSEAPLYPIIHLEGHDIEVAFT
HGGQYGEEHYSFVNGQHTTQGGTHQAAFREAIVKTLRDFFKKNYEAGDIRSSLVAAISIKVKEPVFESQTKTKLGSNTIS
PEGETIRTFVGNFIARELDNYLHKNPETAKALEAKIKDSERDRKELSGIQKLARENARKAKIHNKNLRDCRVHYNSKHSR
AGETTLFITEGISASGSITTARDAETQAVFSLRGKPLNSFGMSRKVVYENEEFNFLQHALNIENGLEELRYDKVVIATDA
DDDGMHIRILLMTFFIQFFPELIREGHLFILQTPLFRVRNKQQTIYCYSDAEREEAISLLGKNPEITRFKGLGEISPQEF
KAFIGEGMRLDRVRLEDSHKVKDLLSFYMGKNTRERQEYILNNLRVELDPVMD
>Mature_612_residues
AYDENSIKTLDWREHIRMRPGMYIGKLGDGASPDDGIYVLLKEVIDNSIDEFVMGFGKKITIDVTPDGLCEVRDFGRGIP
LGKLLDCAAKINTGAKYDSDAFKKSVGLNGVGIKAVNALSDFFEIQAYRDGETRSYQFCRGKEIPKGRKDGATEEPNGTR
TAFHADPDIFPAATQFRPEYIEKMCRYYSYLNKGLALHFNGRRYISKNGLLDLLAEAMSEAPLYPIIHLEGHDIEVAFTH
GGQYGEEHYSFVNGQHTTQGGTHQAAFREAIVKTLRDFFKKNYEAGDIRSSLVAAISIKVKEPVFESQTKTKLGSNTISP
EGETIRTFVGNFIARELDNYLHKNPETAKALEAKIKDSERDRKELSGIQKLARENARKAKIHNKNLRDCRVHYNSKHSRA
GETTLFITEGISASGSITTARDAETQAVFSLRGKPLNSFGMSRKVVYENEEFNFLQHALNIENGLEELRYDKVVIATDAD
DDGMHIRILLMTFFIQFFPELIREGHLFILQTPLFRVRNKQQTIYCYSDAEREEAISLLGKNPEITRFKGLGEISPQEFK
AFIGEGMRLDRVRLEDSHKVKDLLSFYMGKNTRERQEYILNNLRVELDPVMD

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=672, Percent_Identity=27.2321428571429, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI19913408, Length=633, Percent_Identity=25.7503949447077, Blast_Score=116, Evalue=7e-26,
Organism=Escherichia coli, GI48994957, Length=567, Percent_Identity=32.6278659611993, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1789408, Length=600, Percent_Identity=30, Blast_Score=244, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17535065, Length=686, Percent_Identity=23.3236151603499, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI212645845, Length=520, Percent_Identity=24.0384615384615, Blast_Score=86, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324241, Length=650, Percent_Identity=24.4615384615385, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17136538, Length=662, Percent_Identity=26.2839879154079, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.3 [C]

Molecular weight: Translated: 69518; Mature: 69386

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYDENSIKTLDWREHIRMRPGMYIGKLGDGASPDDGIYVLLKEVIDNSIDEFVMGFGKK
CCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCE
ITIDVTPDGLCEVRDFGRGIPLGKLLDCAAKINTGAKYDSDAFKKSVGLNGVGIKAVNAL
EEEEECCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHH
SDFFEIQAYRDGETRSYQFCRGKEIPKGRKDGATEEPNGTRTAFHADPDIFPAATQFRPE
HHHHHHEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCHH
YIEKMCRYYSYLNKGLALHFNGRRYISKNGLLDLLAEAMSEAPLYPIIHLEGHDIEVAFT
HHHHHHHHHHHHCCCEEEEECCCEEECCCCHHHHHHHHHHCCCCCEEEEECCCEEEEEEE
HGGQYGEEHYSFVNGQHTTQGGTHQAAFREAIVKTLRDFFKKNYEAGDIRSSLVAAISIK
CCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEE
VKEPVFESQTKTKLGSNTISPEGETIRTFVGNFIARELDNYLHKNPETAKALEAKIKDSE
ECCCCHHCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCH
RDRKELSGIQKLARENARKAKIHNKNLRDCRVHYNSKHSRAGETTLFITEGISASGSITT
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCEEE
ARDAETQAVFSLRGKPLNSFGMSRKVVYENEEFNFLQHALNIENGLEELRYDKVVIATDA
CCCCHHHHHHHCCCCCCHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DDDGMHIRILLMTFFIQFFPELIREGHLFILQTPLFRVRNKQQTIYCYSDAEREEAISLL
CCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCEEEEECCCCHHHHHHHH
GKNPEITRFKGLGEISPQEFKAFIGEGMRLDRVRLEDSHKVKDLLSFYMGKNTRERQEYI
CCCCCCEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHH
LNNLRVELDPVMD
HHCCEEEECCCCC
>Mature Secondary Structure 
AYDENSIKTLDWREHIRMRPGMYIGKLGDGASPDDGIYVLLKEVIDNSIDEFVMGFGKK
CCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCE
ITIDVTPDGLCEVRDFGRGIPLGKLLDCAAKINTGAKYDSDAFKKSVGLNGVGIKAVNAL
EEEEECCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHH
SDFFEIQAYRDGETRSYQFCRGKEIPKGRKDGATEEPNGTRTAFHADPDIFPAATQFRPE
HHHHHHEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCHH
YIEKMCRYYSYLNKGLALHFNGRRYISKNGLLDLLAEAMSEAPLYPIIHLEGHDIEVAFT
HHHHHHHHHHHHCCCEEEEECCCEEECCCCHHHHHHHHHHCCCCCEEEEECCCEEEEEEE
HGGQYGEEHYSFVNGQHTTQGGTHQAAFREAIVKTLRDFFKKNYEAGDIRSSLVAAISIK
CCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEE
VKEPVFESQTKTKLGSNTISPEGETIRTFVGNFIARELDNYLHKNPETAKALEAKIKDSE
ECCCCHHCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCH
RDRKELSGIQKLARENARKAKIHNKNLRDCRVHYNSKHSRAGETTLFITEGISASGSITT
HHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCEEE
ARDAETQAVFSLRGKPLNSFGMSRKVVYENEEFNFLQHALNIENGLEELRYDKVVIATDA
CCCCHHHHHHHCCCCCCHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DDDGMHIRILLMTFFIQFFPELIREGHLFILQTPLFRVRNKQQTIYCYSDAEREEAISLL
CCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCEEEEECCCCHHHHHHHH
GKNPEITRFKGLGEISPQEFKAFIGEGMRLDRVRLEDSHKVKDLLSFYMGKNTRERQEYI
CCCCCCEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHH
LNNLRVELDPVMD
HHCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double stranded DNA; DNA [C]

Specific reaction: ATP dependent breakage followed by passage and rejoining of double stranded DNA Protein + DNA = Protein-DNA [C]

General reaction: Isomerization [C]

Inhibitor: CJ-12, 371; CJ-12, 372; Coumermycin; Doxorubicin; Sitafloxacin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 7812434 [H]