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The map label for this gene is phoB [H]

Identifier: 187735605

GI number: 187735605

Start: 1325543

End: 1326247

Strand: Reverse

Name: phoB [H]

Synonym: Amuc_1110

Alternate gene names: 187735605

Gene position: 1326247-1325543 (Counterclockwise)

Preceding gene: 187735608

Following gene: 187735604

Centisome position: 49.78

GC content: 60.14

Gene sequence:

>705_bases
ATGGCAACCATTCTTATCGCTGAAGACGACCACGCCATTTCAGATCTTGTGGCTTATAATCTGGAACGTGCGGGCCATAC
GCCCCTGGCGGCCTATGACGGCCTGACCGCGCTGGAAGTGGCGCGCCGGGACAAGCCGGAACTGATCCTGCTGGACCAGA
TGATGCCGGGCATGGACGGACACGGCGTACTGAGGGAACTGCGCCGGGACAGCCGCACCCAGAACATTCCCGTCATCTTC
CTGACAGCCAAGGCCCAGGCGGAAGACCGCATCCAGGGGCTGGAACTGGGAGCGGACGACTATGTGACCAAACCGTTCAG
CCCCAAGGAACTTGTCCTGCGCGTGGCCAGCGTGCTGAAACGGTGCGAGCACACGCCGGGCAGCGTCATTGCGGAATACG
GCCCCTTCACTTTTGACAAAAACGCCCTCAAATTCTATATCGACAAGGAGGAAGTAGACCTGACGGCCACGGAATTCAAG
CTCATGATCCATATGGTTGAACGGGAGGGCCAGACCCTGAACCGCAACGACCTGCTCAGTTGCGTATGGGGGTACAGCCA
CCAGGCCCAGAGCCGTACGCTGGACACGCACATGAAGCGGCTGCGCCAGAAACTGGCTCCCTACGCGGACTGCATTGAAA
CCGTCCGCAGCATCGGCTACCGCTTTACCCTGCCGGGACGCCGCATCCCGGAGGAAAAGGCTTAA

Upstream 100 bases:

>100_bases
GCTTTAACTGTCCGGAACCGCCTCCCGGGCGGGGAAATGTGAACGAATCGTCACATCTAATATCGGGACACCGCCCTTTC
TTTCATCGTAAAAAACAGAC

Downstream 100 bases:

>100_bases
CACTCCGGTTCCATGTCGGCCATCGACTACATCCTAACCATCCTTATTCTGGCCTCCCTGTACCTGAACTGGCATCTGGT
CCAGGTATGTCGGTCCGCCA

Product: two component transcriptional regulator, winged helix family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MATILIAEDDHAISDLVAYNLERAGHTPLAAYDGLTALEVARRDKPELILLDQMMPGMDGHGVLRELRRDSRTQNIPVIF
LTAKAQAEDRIQGLELGADDYVTKPFSPKELVLRVASVLKRCEHTPGSVIAEYGPFTFDKNALKFYIDKEEVDLTATEFK
LMIHMVEREGQTLNRNDLLSCVWGYSHQAQSRTLDTHMKRLRQKLAPYADCIETVRSIGYRFTLPGRRIPEEKA

Sequences:

>Translated_234_residues
MATILIAEDDHAISDLVAYNLERAGHTPLAAYDGLTALEVARRDKPELILLDQMMPGMDGHGVLRELRRDSRTQNIPVIF
LTAKAQAEDRIQGLELGADDYVTKPFSPKELVLRVASVLKRCEHTPGSVIAEYGPFTFDKNALKFYIDKEEVDLTATEFK
LMIHMVEREGQTLNRNDLLSCVWGYSHQAQSRTLDTHMKRLRQKLAPYADCIETVRSIGYRFTLPGRRIPEEKA
>Mature_233_residues
ATILIAEDDHAISDLVAYNLERAGHTPLAAYDGLTALEVARRDKPELILLDQMMPGMDGHGVLRELRRDSRTQNIPVIFL
TAKAQAEDRIQGLELGADDYVTKPFSPKELVLRVASVLKRCEHTPGSVIAEYGPFTFDKNALKFYIDKEEVDLTATEFKL
MIHMVEREGQTLNRNDLLSCVWGYSHQAQSRTLDTHMKRLRQKLAPYADCIETVRSIGYRFTLPGRRIPEEKA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=222, Percent_Identity=39.1891891891892, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1788394, Length=232, Percent_Identity=35.7758620689655, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=35.8744394618834, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=36.4864864864865, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1789809, Length=234, Percent_Identity=31.1965811965812, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=32.1428571428571, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1786911, Length=230, Percent_Identity=36.0869565217391, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=31.6017316017316, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI2367329, Length=230, Percent_Identity=35.2173913043478, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=30.4932735426009, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1789402, Length=217, Percent_Identity=30.4147465437788, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1790552, Length=221, Percent_Identity=31.2217194570136, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI145693140, Length=239, Percent_Identity=30.9623430962343, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787229, Length=225, Percent_Identity=30.6666666666667, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788550, Length=117, Percent_Identity=36.7521367521368, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26528; Mature: 26397

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATILIAEDDHAISDLVAYNLERAGHTPLAAYDGLTALEVARRDKPELILLDQMMPGMDG
CEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHCCCCCEEEECCCCCCCCC
HGVLRELRRDSRTQNIPVIFLTAKAQAEDRIQGLELGADDYVTKPFSPKELVLRVASVLK
HHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
RCEHTPGSVIAEYGPFTFDKNALKFYIDKEEVDLTATEFKLMIHMVEREGQTLNRNDLLS
HHCCCCCCEEECCCCCEECCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
CVWGYSHQAQSRTLDTHMKRLRQKLAPYADCIETVRSIGYRFTLPGRRIPEEKA
HHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
ATILIAEDDHAISDLVAYNLERAGHTPLAAYDGLTALEVARRDKPELILLDQMMPGMDG
EEEEEECCCHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHCCCCCEEEECCCCCCCCC
HGVLRELRRDSRTQNIPVIFLTAKAQAEDRIQGLELGADDYVTKPFSPKELVLRVASVLK
HHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
RCEHTPGSVIAEYGPFTFDKNALKFYIDKEEVDLTATEFKLMIHMVEREGQTLNRNDLLS
HHCCCCCCEEECCCCCEECCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
CVWGYSHQAQSRTLDTHMKRLRQKLAPYADCIETVRSIGYRFTLPGRRIPEEKA
HHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]