Click here to switch to the map view.

The map label for this gene is rlmB [H]

Identifier: 187735575

GI number: 187735575

Start: 1291233

End: 1291814

Strand: Direct

Name: rlmB [H]

Synonym: Amuc_1080

Alternate gene names: 187735575

Gene position: 1291233-1291814 (Clockwise)

Preceding gene: 187735570

Following gene: 187735576

Centisome position: 48.47

GC content: 60.82

Gene sequence:

>582_bases
ATGGGCATGAGACCCCAAGGGAACGAACAGGAAGCGCGCCGGAACAAGCATGTGCGCAGCGCGCCGTCCAAAATTGCCGT
GAAGGCGCTGGGAGAGGAGGTGCTGGACGGGATTCTGGACAAATCCTCCAACCCTCTGCTGCTTATTCTGGACTGCGTGC
AGGACCCCCACAACCTGGGAGCCATCCTGCGGACGGCGGACGGCGCGGGAGTGGATGCCGTCATCGCCCCCCGGGACAAA
TCCGTGGGCATTACGGAGACGGTGCTGCGCGTTTCCGTCGGCGCTGCGGAAAAGGTGCCTTTTATTCAGGTGACCAATCT
GGCGCGCACCATGAAGCAGCTCCAGAGCCGCGGCATCTGGATATTCGGCACGTCGGACAAAGGAGACCGCGGCCTGTATG
AAACGGACTTCACGGGGCCGGCCGCACTGGTGATGGGGGCGGAAGGGGAAGGGATGAGAAGATTGACGGAAGAGAACTGC
GATTTCCTGCTGAGGATTCCCATGAAGGGAAGCATTCCGTGCCTGAACGTATCTGTGGCTACGGGCGTATGCCTGTATGA
AATGGTGCGGCAGCGTTCCTGA

Upstream 100 bases:

>100_bases
TGGTACGGAATTGTACCATGACGCGGGCTGCCAAGTCACGCCCAATGATGGGGCGGCAGGGCATATTTGTCTGGAAAAGA
AGTGTGAAACACGCTAATTC

Downstream 100 bases:

>100_bases
TTCCATGACTGTTTTTCTCCCTCCCTCGTCTTTATGATTCAGGAACTTGATCTTGCTCCGGGGGAACGCGCCAGGTTTAT
TTCAGACATCCATTTCGGCC

Product: RNA methyltransferase, TrmH family, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MGMRPQGNEQEARRNKHVRSAPSKIAVKALGEEVLDGILDKSSNPLLLILDCVQDPHNLGAILRTADGAGVDAVIAPRDK
SVGITETVLRVSVGAAEKVPFIQVTNLARTMKQLQSRGIWIFGTSDKGDRGLYETDFTGPAALVMGAEGEGMRRLTEENC
DFLLRIPMKGSIPCLNVSVATGVCLYEMVRQRS

Sequences:

>Translated_193_residues
MGMRPQGNEQEARRNKHVRSAPSKIAVKALGEEVLDGILDKSSNPLLLILDCVQDPHNLGAILRTADGAGVDAVIAPRDK
SVGITETVLRVSVGAAEKVPFIQVTNLARTMKQLQSRGIWIFGTSDKGDRGLYETDFTGPAALVMGAEGEGMRRLTEENC
DFLLRIPMKGSIPCLNVSVATGVCLYEMVRQRS
>Mature_192_residues
GMRPQGNEQEARRNKHVRSAPSKIAVKALGEEVLDGILDKSSNPLLLILDCVQDPHNLGAILRTADGAGVDAVIAPRDKS
VGITETVLRVSVGAAEKVPFIQVTNLARTMKQLQSRGIWIFGTSDKGDRGLYETDFTGPAALVMGAEGEGMRRLTEENCD
FLLRIPMKGSIPCLNVSVATGVCLYEMVRQRS

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=162, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1790623, Length=162, Percent_Identity=60.4938271604938, Blast_Score=200, Evalue=5e-53,
Organism=Escherichia coli, GI1788935, Length=164, Percent_Identity=29.2682926829268, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790083, Length=157, Percent_Identity=32.484076433121, Blast_Score=69, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=170, Percent_Identity=32.3529411764706, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 20876; Mature: 20745

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMRPQGNEQEARRNKHVRSAPSKIAVKALGEEVLDGILDKSSNPLLLILDCVQDPHNLG
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHH
AILRTADGAGVDAVIAPRDKSVGITETVLRVSVGAAEKVPFIQVTNLARTMKQLQSRGIW
HEEEECCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEE
IFGTSDKGDRGLYETDFTGPAALVMGAEGEGMRRLTEENCDFLLRIPMKGSIPCLNVSVA
EEECCCCCCCCCEECCCCCCEEEEECCCCCCHHHHHHCCCCEEEEECCCCCCCEEEHHHH
TGVCLYEMVRQRS
HHHHHHHHHHHCC
>Mature Secondary Structure 
GMRPQGNEQEARRNKHVRSAPSKIAVKALGEEVLDGILDKSSNPLLLILDCVQDPHNLG
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHH
AILRTADGAGVDAVIAPRDKSVGITETVLRVSVGAAEKVPFIQVTNLARTMKQLQSRGIW
HEEEECCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEE
IFGTSDKGDRGLYETDFTGPAALVMGAEGEGMRRLTEENCDFLLRIPMKGSIPCLNVSVA
EEECCCCCCCCCEECCCCCCEEEEECCCCCCHHHHHHCCCCEEEEECCCCCCCEEEHHHH
TGVCLYEMVRQRS
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]