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The map label for this gene is rfaJ [H]

Identifier: 187735440

GI number: 187735440

Start: 1118530

End: 1119645

Strand: Reverse

Name: rfaJ [H]

Synonym: Amuc_0939

Alternate gene names: 187735440

Gene position: 1119645-1118530 (Counterclockwise)

Preceding gene: 187735441

Following gene: 187735424

Centisome position: 42.03

GC content: 58.15

Gene sequence:

>1116_bases
ATGAAATTCCCCGATGCCATGACAACCCCGCCCGTTCCCGCATCCCCGGAGAAATCACGCATCCCCGTCATGTTTTCCGC
CACCGGCGGCTGGGGCCTGCCCCTGGGCGTAGCCATTCACACCCTTTGCCTTCACGCCAGTTCCGGACGCTTTTATGACA
TTCACATCGTTCACGACGGAATGGACGCGCGAATAATACAGGAGCTGAACCAGGTTGCCGCCCCCTTTCCGCAGGTTTCC
CTTTCTTTCCTGCAACTTCCGGAAGAATTCCGCCATCTCTTTCAAAACGGCAACAAGGACCGCTACTCCCCCCTTGCGTA
TGCCCGCCTGATGGCCGGCAGCCTGTTCCCGCAGTACGGCAGGATCGTTTATCTGGACGCAGATGTCCTGCTGGCCGGAG
ACGTAGCCGAACTGTATTTTTCCGATTTGCGGGGAGCTTCCGTAGCGGCGGCCGGAGACGGCCTGGCCCTCTGGAGCATT
GAAAAAGGAACGATGCACCCCCATCTGGAATATATGGGCAACTACCTTTCCTTCCCCCTTTCCTACTGCAATTCCGGCGT
CCTGGTGCTGGATCTGGACCAGATGCGCCGCCGCAACCTGGAACACCGGCTGCTCCAACAGCTCCGGAGCCGCCCGGACC
CCTTCCCCTATCCGGACCAGGACATCTTAAATATCGCCCTGCACGGAGACATGACGACGCTGCCTCCGGAATGGAACTTC
CAATTCCTGTCCTGGACCTGGGATGAAGAAAAAACACGGCTCCTGCGCGGAACCGAATTTGAAAACGTTCCGACCATATC
CTGCGGGCGTTCATGGAAACTGCTGCACATGGTAGGCCCGGAAAAACCATGGCGGCTCCCTGACACCCCCGGAACCATGG
GGCAGTTCCACTGGATCCTGTACTCCTTTTTCTGGTGGCCGGAAGCAAAGAGGCTTCCCGTGTTCCGGGAGGAACTGGAT
GCGATTTCCCAGGGGCTGGCCCCGCTCCTCCAGCGCCATATCCGCGGCCAGCAATGGAAACTGTTCTTCTCCCGGGGCCA
TATTTTCCGGAAACGCCGGGACAAGATCAGGTGGCTGAAAAAATTGCTGTCCATTCTTGACGGCAGAAAACCGTAA

Upstream 100 bases:

>100_bases
TCAAATAACAGACATCCCTTTTCCGGCCTGAACGGAGCCTCCATGCTCTTTCCCTCCTCGGCCTATTCCTTCCCGCAGCC
CCTTCTTCAAGACCCTTCCC

Downstream 100 bases:

>100_bases
CCGGCTTCCCCCCTCTCCGGGAAACATGGGCAGCCTCCGCAGACGGCCGGAAAACCGGCGTACTCCGGAGACTGTTCCCG
GCGGACCTTCAAACGCCCGT

Product: glycosyl transferase family 8

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKFPDAMTTPPVPASPEKSRIPVMFSATGGWGLPLGVAIHTLCLHASSGRFYDIHIVHDGMDARIIQELNQVAAPFPQVS
LSFLQLPEEFRHLFQNGNKDRYSPLAYARLMAGSLFPQYGRIVYLDADVLLAGDVAELYFSDLRGASVAAAGDGLALWSI
EKGTMHPHLEYMGNYLSFPLSYCNSGVLVLDLDQMRRRNLEHRLLQQLRSRPDPFPYPDQDILNIALHGDMTTLPPEWNF
QFLSWTWDEEKTRLLRGTEFENVPTISCGRSWKLLHMVGPEKPWRLPDTPGTMGQFHWILYSFFWWPEAKRLPVFREELD
AISQGLAPLLQRHIRGQQWKLFFSRGHIFRKRRDKIRWLKKLLSILDGRKP

Sequences:

>Translated_371_residues
MKFPDAMTTPPVPASPEKSRIPVMFSATGGWGLPLGVAIHTLCLHASSGRFYDIHIVHDGMDARIIQELNQVAAPFPQVS
LSFLQLPEEFRHLFQNGNKDRYSPLAYARLMAGSLFPQYGRIVYLDADVLLAGDVAELYFSDLRGASVAAAGDGLALWSI
EKGTMHPHLEYMGNYLSFPLSYCNSGVLVLDLDQMRRRNLEHRLLQQLRSRPDPFPYPDQDILNIALHGDMTTLPPEWNF
QFLSWTWDEEKTRLLRGTEFENVPTISCGRSWKLLHMVGPEKPWRLPDTPGTMGQFHWILYSFFWWPEAKRLPVFREELD
AISQGLAPLLQRHIRGQQWKLFFSRGHIFRKRRDKIRWLKKLLSILDGRKP
>Mature_371_residues
MKFPDAMTTPPVPASPEKSRIPVMFSATGGWGLPLGVAIHTLCLHASSGRFYDIHIVHDGMDARIIQELNQVAAPFPQVS
LSFLQLPEEFRHLFQNGNKDRYSPLAYARLMAGSLFPQYGRIVYLDADVLLAGDVAELYFSDLRGASVAAAGDGLALWSI
EKGTMHPHLEYMGNYLSFPLSYCNSGVLVLDLDQMRRRNLEHRLLQQLRSRPDPFPYPDQDILNIALHGDMTTLPPEWNF
QFLSWTWDEEKTRLLRGTEFENVPTISCGRSWKLLHMVGPEKPWRLPDTPGTMGQFHWILYSFFWWPEAKRLPVFREELD
AISQGLAPLLQRHIRGQQWKLFFSRGHIFRKRRDKIRWLKKLLSILDGRKP

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790056, Length=164, Percent_Identity=32.3170731707317, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.58 [H]

Molecular weight: Translated: 42738; Mature: 42738

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFPDAMTTPPVPASPEKSRIPVMFSATGGWGLPLGVAIHTLCLHASSGRFYDIHIVHDG
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECC
MDARIIQELNQVAAPFPQVSLSFLQLPEEFRHLFQNGNKDRYSPLAYARLMAGSLFPQYG
CCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
RIVYLDADVLLAGDVAELYFSDLRGASVAAAGDGLALWSIEKGTMHPHLEYMGNYLSFPL
CEEEEECCEEEECCHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHH
SYCNSGVLVLDLDQMRRRNLEHRLLQQLRSRPDPFPYPDQDILNIALHGDMTTLPPEWNF
HHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHEEEEEECCCCCCCCCCCE
QFLSWTWDEEKTRLLRGTEFENVPTISCGRSWKLLHMVGPEKPWRLPDTPGTMGQFHWIL
EEEEEECCHHHHHHHCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHH
YSFFWWPEAKRLPVFREELDAISQGLAPLLQRHIRGQQWKLFFSRGHIFRKRRDKIRWLK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHH
KLLSILDGRKP
HHHHHHCCCCC
>Mature Secondary Structure
MKFPDAMTTPPVPASPEKSRIPVMFSATGGWGLPLGVAIHTLCLHASSGRFYDIHIVHDG
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECC
MDARIIQELNQVAAPFPQVSLSFLQLPEEFRHLFQNGNKDRYSPLAYARLMAGSLFPQYG
CCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
RIVYLDADVLLAGDVAELYFSDLRGASVAAAGDGLALWSIEKGTMHPHLEYMGNYLSFPL
CEEEEECCEEEECCHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHH
SYCNSGVLVLDLDQMRRRNLEHRLLQQLRSRPDPFPYPDQDILNIALHGDMTTLPPEWNF
HHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHEEEEEECCCCCCCCCCCE
QFLSWTWDEEKTRLLRGTEFENVPTISCGRSWKLLHMVGPEKPWRLPDTPGTMGQFHWIL
EEEEEECCHHHHHHHCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHH
YSFFWWPEAKRLPVFREELDAISQGLAPLLQRHIRGQQWKLFFSRGHIFRKRRDKIRWLK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHH
KLLSILDGRKP
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1624461; 8041620; 9278503 [H]