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The map label for this gene is pdhC [H]

Identifier: 187735387

GI number: 187735387

Start: 1053894

End: 1055168

Strand: Reverse

Name: pdhC [H]

Synonym: Amuc_0885

Alternate gene names: 187735387

Gene position: 1055168-1053894 (Counterclockwise)

Preceding gene: 187735388

Following gene: 187735386

Centisome position: 39.61

GC content: 64.0

Gene sequence:

>1275_bases
ATGCCTAAAGTACCCATTCTGATGCCCCAGCTCGGGGATTCCATTGCGGAAGCCACTGTGCTGCGCCTGCTGGCGGCCCA
GGGCGATACCGTAGAAGCCGACCAGGAAATCTTTGAGGTAGAAACCAACAAGGCCACCATGGGAGTCACCACCATGTGCG
GCGGCATCCTGAGCGATGTGTTCATCAAGGAAGGGGAATCCGTCGTGGTCGGCGCCTGCATGGCCATGATTGAGGCCACG
GAAGAGGAAATTGAGCGTTCCGGAGCGACTCCGGCCGGAGATTCCACCCAGCCGTCCCTTCCAGCCAGTCCGGAATCCGT
TCCCCAGGCCGCGCCCTCCGCCCCTCCCCGGGAGGAAAAACCCGCAGGCGTCCACTTCGGGGTGACGGGGGAATCCTATC
AGGAAAACGGAACGGACCTGAAAGTCCAGCCCAGCGTGCGCGGCCTCCCCGTACCCGCCGGCATGAAGGGCGCGCACTAC
ATGTCCCCCCGGATGAAGGCGCGCATGGATGAATTGGGCATGAGCGCCTCCGACATCGCTTTCATCTCCGGTTCCGGGGC
GGGCGGACGCGTCACCATTGACGACCTGGAAGAATTCCTGGAATATGTTAGCCAATGGCCGCACCGCAAGGCCTCATCCA
TGCGGCTGGCCGTGGCGGACGCCATGCGCCGCAGCTGGACGCGCCCGCTGGCCTCCGCCGGGCGTCCCGTATTCATGGAC
CCCCTCATCAAACACAGGCAAAATTCCCCGCTGCGGCCCGGCATCACCCTGTACTTTGCCCGCGCCCTGGCCCTGTCCCT
GGCGGAAAGCCCGGAATGCGCCGGCTACCTGGTGGGGGAAAACATTCTTTCTCCCAAAACAATCGACATCGGCATCGCCG
CCCAGGTGGCGGACGGCGTCATGGTTCCCGTGCTGCGCCGCGTGAACGAACGGACGATGGAAGAACTGCTGGAAGACTAT
AACAGGCTGATTGCCCAGGCGCGCCGCCGCAGGCTGGCTCCGGAAGACAGCACGGGCGGCATCGCCACGGTCACCAACTT
CGGCGGCTTCGGCCTCACCTTTGCCGCGCCCATGCCCATGCCCAGCGAATCCATTATCCTGGGGGTGGGGGCCGTCACCA
AAACCCCGGTCTGGAGTGACGAAGTGGAGGCGTTTATCCCCATCTCCAAGGCCAACATCGTGGCTACGGGAGACCACCGC
GTGGTGGACGGAGCGGACATCGGCCGCCTGCTCAAGCGCGTGGCGGAATTGCTCCAGCGCCCGGAATACCTGTAA

Upstream 100 bases:

>100_bases
CCAGCACCCCAACCTCTGGAAGGCGCACCGCCCCACGCTGGAATCCATTGCCGCCTCCATCCGCCATCTTCTATCCATTT
GATTCAACCCAGTTTCCATC

Downstream 100 bases:

>100_bases
CCAGCCCCGTTCCCCCAGCTTCACGCCGCATGACAACAGACCCGCCCGTATTAGGCCTGGTAGCCGGAGACGGCGTTTAT
CCGGAATATATTGTCCGGGG

Product: catalytic domain of components of various dehydrogenase complexes

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MPKVPILMPQLGDSIAEATVLRLLAAQGDTVEADQEIFEVETNKATMGVTTMCGGILSDVFIKEGESVVVGACMAMIEAT
EEEIERSGATPAGDSTQPSLPASPESVPQAAPSAPPREEKPAGVHFGVTGESYQENGTDLKVQPSVRGLPVPAGMKGAHY
MSPRMKARMDELGMSASDIAFISGSGAGGRVTIDDLEEFLEYVSQWPHRKASSMRLAVADAMRRSWTRPLASAGRPVFMD
PLIKHRQNSPLRPGITLYFARALALSLAESPECAGYLVGENILSPKTIDIGIAAQVADGVMVPVLRRVNERTMEELLEDY
NRLIAQARRRRLAPEDSTGGIATVTNFGGFGLTFAAPMPMPSESIILGVGAVTKTPVWSDEVEAFIPISKANIVATGDHR
VVDGADIGRLLKRVAELLQRPEYL

Sequences:

>Translated_424_residues
MPKVPILMPQLGDSIAEATVLRLLAAQGDTVEADQEIFEVETNKATMGVTTMCGGILSDVFIKEGESVVVGACMAMIEAT
EEEIERSGATPAGDSTQPSLPASPESVPQAAPSAPPREEKPAGVHFGVTGESYQENGTDLKVQPSVRGLPVPAGMKGAHY
MSPRMKARMDELGMSASDIAFISGSGAGGRVTIDDLEEFLEYVSQWPHRKASSMRLAVADAMRRSWTRPLASAGRPVFMD
PLIKHRQNSPLRPGITLYFARALALSLAESPECAGYLVGENILSPKTIDIGIAAQVADGVMVPVLRRVNERTMEELLEDY
NRLIAQARRRRLAPEDSTGGIATVTNFGGFGLTFAAPMPMPSESIILGVGAVTKTPVWSDEVEAFIPISKANIVATGDHR
VVDGADIGRLLKRVAELLQRPEYL
>Mature_423_residues
PKVPILMPQLGDSIAEATVLRLLAAQGDTVEADQEIFEVETNKATMGVTTMCGGILSDVFIKEGESVVVGACMAMIEATE
EEIERSGATPAGDSTQPSLPASPESVPQAAPSAPPREEKPAGVHFGVTGESYQENGTDLKVQPSVRGLPVPAGMKGAHYM
SPRMKARMDELGMSASDIAFISGSGAGGRVTIDDLEEFLEYVSQWPHRKASSMRLAVADAMRRSWTRPLASAGRPVFMDP
LIKHRQNSPLRPGITLYFARALALSLAESPECAGYLVGENILSPKTIDIGIAAQVADGVMVPVLRRVNERTMEELLEDYN
RLIAQARRRRLAPEDSTGGIATVTNFGGFGLTFAAPMPMPSESIILGVGAVTKTPVWSDEVEAFIPISKANIVATGDHRV
VDGADIGRLLKRVAELLQRPEYL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=449, Percent_Identity=23.8307349665924, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI110671329, Length=469, Percent_Identity=22.8144989339019, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI19923748, Length=185, Percent_Identity=29.1891891891892, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI203098816, Length=221, Percent_Identity=24.4343891402715, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI203098753, Length=218, Percent_Identity=24.3119266055046, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786946, Length=453, Percent_Identity=24.2825607064018, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786305, Length=177, Percent_Identity=30.5084745762712, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17537937, Length=460, Percent_Identity=24.1304347826087, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17560088, Length=459, Percent_Identity=26.797385620915, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25146366, Length=437, Percent_Identity=24.2562929061785, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324258, Length=468, Percent_Identity=22.4358974358974, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320352, Length=431, Percent_Identity=22.737819025522, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24582497, Length=222, Percent_Identity=25.6756756756757, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129315, Length=222, Percent_Identity=25.6756756756757, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24645909, Length=172, Percent_Identity=28.4883720930233, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45379; Mature: 45247

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKVPILMPQLGDSIAEATVLRLLAAQGDTVEADQEIFEVETNKATMGVTTMCGGILSDV
CCCCCEECHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHEEECCCCCCCHHHHHHHHHHHH
FIKEGESVVVGACMAMIEATEEEIERSGATPAGDSTQPSLPASPESVPQAAPSAPPREEK
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAGVHFGVTGESYQENGTDLKVQPSVRGLPVPAGMKGAHYMSPRMKARMDELGMSASDIA
CCCEEECCCCCCHHCCCCCEEECCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCEE
FISGSGAGGRVTIDDLEEFLEYVSQWPHRKASSMRLAVADAMRRSWTRPLASAGRPVFMD
EEECCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCEEHH
PLIKHRQNSPLRPGITLYFARALALSLAESPECAGYLVGENILSPKTIDIGIAAQVADGV
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEHHHHHCCH
MVPVLRRVNERTMEELLEDYNRLIAQARRRRLAPEDSTGGIATVTNFGGFGLTFAAPMPM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCCCC
PSESIILGVGAVTKTPVWSDEVEAFIPISKANIVATGDHRVVDGADIGRLLKRVAELLQR
CCCCEEEEECCCCCCCCCCCCCCEEEEECCCCEEECCCCEEECCHHHHHHHHHHHHHHHC
PEYL
CCCC
>Mature Secondary Structure 
PKVPILMPQLGDSIAEATVLRLLAAQGDTVEADQEIFEVETNKATMGVTTMCGGILSDV
CCCCEECHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHEEECCCCCCCHHHHHHHHHHHH
FIKEGESVVVGACMAMIEATEEEIERSGATPAGDSTQPSLPASPESVPQAAPSAPPREEK
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAGVHFGVTGESYQENGTDLKVQPSVRGLPVPAGMKGAHYMSPRMKARMDELGMSASDIA
CCCEEECCCCCCHHCCCCCEEECCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCCCCCEE
FISGSGAGGRVTIDDLEEFLEYVSQWPHRKASSMRLAVADAMRRSWTRPLASAGRPVFMD
EEECCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCEEHH
PLIKHRQNSPLRPGITLYFARALALSLAESPECAGYLVGENILSPKTIDIGIAAQVADGV
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEHHHHHCCH
MVPVLRRVNERTMEELLEDYNRLIAQARRRRLAPEDSTGGIATVTNFGGFGLTFAAPMPM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCCCC
PSESIILGVGAVTKTPVWSDEVEAFIPISKANIVATGDHRVVDGADIGRLLKRVAELLQR
CCCCEEEEECCCCCCCCCCCCCCEEEEECCCCEEECCCCEEECCHHHHHHHHHHHHHHHC
PEYL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]