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The map label for this gene is phoA [H]

Identifier: 187735372

GI number: 187735372

Start: 1039893

End: 1041038

Strand: Reverse

Name: phoA [H]

Synonym: Amuc_0870

Alternate gene names: 187735372

Gene position: 1041038-1039893 (Counterclockwise)

Preceding gene: 187735373

Following gene: 187735370

Centisome position: 39.08

GC content: 59.34

Gene sequence:

>1146_bases
ATGACTTCTCCGCTGCGCCTTTTAGCCTTTGCCCTCCTTCTTGCTCCGTCCCTGCTTCCGGCGGCGGAAAACCAGCGCTT
CATTCTGGAACGCCCCTATCCGGTGGCGGAAATCCCGGAACCTGACCGCAGCCTTCCCGTCACCAACGTAATCCTGATGA
TTGGAGACGGCATGGGCATCCACCACCTTTCCGCCGCATGGGCAGCCAACAGAGGACGCTTATTTATTGAGAACTGCCCG
GTTACGGGTATTTCCAAAACATGGTGCGCGGACAAGCTGGTCACGGACTCCGCCGCTGCAGGCACCGCCATGGCGACGGG
AACCAAGACGCTTTACCATCGAGTCGCCGTATGCCCCAAGGGCAACAGGCTGGATTCCCTGGTGGACAAGGCGGCGGACA
TGGGCAAATCCACCGGCGTTATCGCCACCTGCGAGCTGAATGACGCCACCCCGGCATCTTTCTGCGCCAATAATGAAAAA
AGATCGGATGCCTACGGCATCATTGGGGATTACCCCCGTTCCCGTGCCGATTTCATCTTTGGCGGCGGCGGCAAATACTT
CACGCAGAGGCCGGACGGCAGGGATATCTTCCAGGAAATGCAGGCGCAGGGATACCGCGTCGCCCGCACCTGGGAAGAAG
CCGGCGCCTTGCCTCCCGGAAAAACACTGGCCGTCACCGCTCCGGGGAATCTTCCCCCGCCCTCCAAGCGGGGAGACGTT
TTCCGCCAAGCCGCACTGAAAGCCCTGGACACCCTTTCCCGGAATCCCGACGGATTTTTCCTGATGATTGAAGGGTCCAA
CATCGACAAGGCAGCCCACAGCAACAACCTGGAAGAAATGATGGAAGAACTCCTTGATTTTGACCGGACTGCGGGAGCCG
TGCTTGACTGGGCCTCCAAACACCCCGGCACCCTGGTCGTCATCACGGCGGACCATAATACGGGGGCATTCGCGCTCACG
GGCGGCAGCCGGGAAAAGGGTGAAATAACGGCCCGGTTCGGTTCCGGCAGTCATGACGGCGTGGCTGTTCCGGTGTACGC
TTTCGGCGCGGGAAGCGGTGCTTTCACCGGCATTTATGAAAATACGGCCATCTTCAGCAAGATCATGGATGCCTTTAAAA
AAGGTGCGGGCAAACCCGTGCGTTAG

Upstream 100 bases:

>100_bases
ACGCCAAATAAGCCCTTTTGAACGGTTCATGCCGGTTCCATACGGGACCGGCATATTTTTTCCTCTTTACTTGTTCCGCC
GCAGACGGGAAAGTGATTGC

Downstream 100 bases:

>100_bases
GTACTCTGTCAAGGCTGTGGCCTGTCCATGCGCCGCGCCACCAGCCCGGCCCCGGCGGTAGCCACGCCGCCTATCAGGAA
CACCGCTTGCGGGCCGCCCG

Product: Alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTSPLRLLAFALLLAPSLLPAAENQRFILERPYPVAEIPEPDRSLPVTNVILMIGDGMGIHHLSAAWAANRGRLFIENCP
VTGISKTWCADKLVTDSAAAGTAMATGTKTLYHRVAVCPKGNRLDSLVDKAADMGKSTGVIATCELNDATPASFCANNEK
RSDAYGIIGDYPRSRADFIFGGGGKYFTQRPDGRDIFQEMQAQGYRVARTWEEAGALPPGKTLAVTAPGNLPPPSKRGDV
FRQAALKALDTLSRNPDGFFLMIEGSNIDKAAHSNNLEEMMEELLDFDRTAGAVLDWASKHPGTLVVITADHNTGAFALT
GGSREKGEITARFGSGSHDGVAVPVYAFGAGSGAFTGIYENTAIFSKIMDAFKKGAGKPVR

Sequences:

>Translated_381_residues
MTSPLRLLAFALLLAPSLLPAAENQRFILERPYPVAEIPEPDRSLPVTNVILMIGDGMGIHHLSAAWAANRGRLFIENCP
VTGISKTWCADKLVTDSAAAGTAMATGTKTLYHRVAVCPKGNRLDSLVDKAADMGKSTGVIATCELNDATPASFCANNEK
RSDAYGIIGDYPRSRADFIFGGGGKYFTQRPDGRDIFQEMQAQGYRVARTWEEAGALPPGKTLAVTAPGNLPPPSKRGDV
FRQAALKALDTLSRNPDGFFLMIEGSNIDKAAHSNNLEEMMEELLDFDRTAGAVLDWASKHPGTLVVITADHNTGAFALT
GGSREKGEITARFGSGSHDGVAVPVYAFGAGSGAFTGIYENTAIFSKIMDAFKKGAGKPVR
>Mature_380_residues
TSPLRLLAFALLLAPSLLPAAENQRFILERPYPVAEIPEPDRSLPVTNVILMIGDGMGIHHLSAAWAANRGRLFIENCPV
TGISKTWCADKLVTDSAAAGTAMATGTKTLYHRVAVCPKGNRLDSLVDKAADMGKSTGVIATCELNDATPASFCANNEKR
SDAYGIIGDYPRSRADFIFGGGGKYFTQRPDGRDIFQEMQAQGYRVARTWEEAGALPPGKTLAVTAPGNLPPPSKRGDVF
RQAALKALDTLSRNPDGFFLMIEGSNIDKAAHSNNLEEMMEELLDFDRTAGAVLDWASKHPGTLVVITADHNTGAFALTG
GSREKGEITARFGSGSHDGVAVPVYAFGAGSGAFTGIYENTAIFSKIMDAFKKGAGKPVR

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=453, Percent_Identity=25.60706401766, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI94721246, Length=345, Percent_Identity=28.9855072463768, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI157266296, Length=345, Percent_Identity=28.9855072463768, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI294660770, Length=398, Percent_Identity=25.6281407035176, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI157266292, Length=371, Percent_Identity=26.9541778975741, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI294660772, Length=354, Percent_Identity=24.8587570621469, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI48994877, Length=409, Percent_Identity=28.8508557457213, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320689, Length=318, Percent_Identity=31.4465408805031, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI18859923, Length=294, Percent_Identity=30.2721088435374, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355981, Length=436, Percent_Identity=27.0642201834862, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21355151, Length=376, Percent_Identity=25.531914893617, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19921912, Length=372, Percent_Identity=25.5376344086022, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355149, Length=329, Percent_Identity=25.8358662613982, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24657835, Length=385, Percent_Identity=27.7922077922078, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI85725200, Length=343, Percent_Identity=25.6559766763848, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI85815833, Length=343, Percent_Identity=25.6559766763848, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24657842, Length=393, Percent_Identity=26.7175572519084, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24651554, Length=359, Percent_Identity=25.9052924791086, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 40611; Mature: 40479

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPLRLLAFALLLAPSLLPAAENQRFILERPYPVAEIPEPDRSLPVTNVILMIGDGMGI
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCH
HHLSAAWAANRGRLFIENCPVTGISKTWCADKLVTDSAAAGTAMATGTKTLYHRVAVCPK
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHCCHHHHHHHEEECCC
GNRLDSLVDKAADMGKSTGVIATCELNDATPASFCANNEKRSDAYGIIGDYPRSRADFIF
CCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
GGGGKYFTQRPDGRDIFQEMQAQGYRVARTWEEAGALPPGKTLAVTAPGNLPPPSKRGDV
CCCCCEECCCCCHHHHHHHHHHCCEEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCHHH
FRQAALKALDTLSRNPDGFFLMIEGSNIDKAAHSNNLEEMMEELLDFDRTAGAVLDWASK
HHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHCCCCCCCHHHHHHCC
HPGTLVVITADHNTGAFALTGGSREKGEITARFGSGSHDGVAVPVYAFGAGSGAFTGIYE
CCCEEEEEEECCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCEEEEHH
NTAIFSKIMDAFKKGAGKPVR
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TSPLRLLAFALLLAPSLLPAAENQRFILERPYPVAEIPEPDRSLPVTNVILMIGDGMGI
CCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCH
HHLSAAWAANRGRLFIENCPVTGISKTWCADKLVTDSAAAGTAMATGTKTLYHRVAVCPK
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHCCHHHHHHHEEECCC
GNRLDSLVDKAADMGKSTGVIATCELNDATPASFCANNEKRSDAYGIIGDYPRSRADFIF
CCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
GGGGKYFTQRPDGRDIFQEMQAQGYRVARTWEEAGALPPGKTLAVTAPGNLPPPSKRGDV
CCCCCEECCCCCHHHHHHHHHHCCEEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCHHH
FRQAALKALDTLSRNPDGFFLMIEGSNIDKAAHSNNLEEMMEELLDFDRTAGAVLDWASK
HHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHCCCCCCCHHHHHHCC
HPGTLVVITADHNTGAFALTGGSREKGEITARFGSGSHDGVAVPVYAFGAGSGAFTGIYE
CCCEEEEEEECCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCEEEEHH
NTAIFSKIMDAFKKGAGKPVR
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]