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The map label for this gene is mnmG [H]

Identifier: 187735314

GI number: 187735314

Start: 955103

End: 956989

Strand: Direct

Name: mnmG [H]

Synonym: Amuc_0812

Alternate gene names: 187735314

Gene position: 955103-956989 (Clockwise)

Preceding gene: 187735313

Following gene: 187735316

Centisome position: 35.85

GC content: 52.2

Gene sequence:

>1887_bases
ATGAACTCCTCCTCTTCTTCCGTTTTTCCCCTGTTTGACGTTCTCGTCGTCGGCGCCGGGCACGCAGGCATTGAGGCGGC
ACTGGCAGCATCCCGTCTGGGGTGTTCCGTCGCCGTCCTAACTCATAACCTGGATACTATCGGACAAATGTCCTGCAATC
CGGCTATTGGGGGACTTGCCAAAGGGCATATCGTCCGGGAAATAGATGCCATGGGGGGAGCGATGGGGTTGAACACGGAT
GCTACCGCCATTCAATTCCGGATGCTGAACGCTTCCAAGGGGCCCAGCGTGCGCGCTCCCCGCGCCCAGTGCGATAAAAC
AGCCTACCGCACAAGAATGAAAATGGTTCTGGAAACCGCCCCCGGCATACGCCTGTTTCAGGGAGATGTGGTCAAACTGC
TCGTCAATGGAGAACAGCAGGCAACGGGAGCCGTTACTTCTCTGGGCTGCGCCATCCATGCCCGCAGCGTGATTATCTGC
TCCGGGACATTCATGAAGGGATTGCTTCATGTGGGCCTTCAACACATAACAGGAGGCCGAATGGGTTGCGCTTCTTCCAG
CCTTTCCGCCGATCTTGCCATGCTGGGTTTTCAGGTGGAAAGGCTTAAAACAGGAACTTCTCCCCGCGTCAATGGAAGAT
CCATCGACTTTTCCGTCTGCGAAATACAGCCCGGAGACGCTCCGGAAGCCCTGTTCAGCTACCGTTCCCGTGATATACTG
AAACGTGATTCCGAACCCTTTTGCACTTTGAACACGTGGGGAGATGAAAATTTCCGGATGAAGCAAATGCCGTGCTGGAT
CACCTACACCAACCAGACTACGCACGACATTATCCGGAAGAACTTAAAATTCAGCCCCCTTTACAGCGGTATCATTGAAG
GCGTAGGCCCCCGGTATTGTCCTTCCATAGAAGATAAAATCGTGCGCTTTGCGGAAAAAAACAGCCATCAGGTATTCCTG
GAACCGGAAGGCCGCAGTACGGAGGAATTCTACCTTAACGGAGTTTCTACTTCACTGCCCTACTCCGCTCAGTGCGACTT
CATCCATACCATTCCCGGCCTGGAAAACGCACAGCTGATCCGTCCGGGATATGCGGTGGAATACGACTTCTGTCCTCCCA
CGCAATTGCATCCTACGCTGGAAACAAAACTGGTGGAAAACCTGTACTTTGCAGGTCAAATCAACGGTACTTCCGGCTAT
GAAGAAGCCGCAGCGCAAGGACTAATGGCGGGAGCGAACGCCGCTCTGAAAACCCTGGGCAAACCTTCTCTGGTGCTGCG
CCGGGATCAGGCTTACATCGGCGTCATGATAGACGACCTTGTTACCAAAGGAACCGATGAACCTTACCGCATGTTTACCA
GCCGGGCAGAATTCCGCCTGTTGCTGCGTCAGGATAATGCAGACCTGCGGTTGGCCCCTCTGGCTTTTGAAGCGGGCCTT
ATTACCTGTGAAATGGCCCGGAAGGCTGAAGACAGGAAAAACGCCGTTGAAGAAGGAATAATAAAAGCTGCCGCGCTTAA
ACATGAAGGAGTTTCTCTCTCCACATGGCTTAAAAGGCCCGGCAATGACTGGACTCTATTGCCTGAAGAACTGAAAAAAA
TGTTCCACGTGGAACATTTGGATGCCATTGCTACGGATATCCAGTACGAAGGGCATATTGAAAGGCAGAAAAAACAGGCG
GAAAAAATCATACGGATGGAAAATAAAACTATTCCCTCCGATATTGATTACAAACATGTCCATGCCTTAAAAAAAGAGGC
TGTCATCAAGCTGGGCAACATACGTCCTGCAACTATCGGGCAGGCCTCCCGCATTCCTGGAATTACCCCGGCAGATATCG
CCCTGTTGCTTGTGCATCTGCAAAAACGCAGCAGGCAACAAACATAA

Upstream 100 bases:

>100_bases
AAACTTTGAAAGAATGCATGAAGGCGGATAAAGCCCTGGTGTCCTCCTCTCTTGATCCCCGCCTGATTCTGCATCGTCTT
GCTATCTGCTCCGCATCCTG

Downstream 100 bases:

>100_bases
TACCCCGCGTTTTTTATTCAAAAATTTCAATTTAAGGAAGACCATAGGAGGCGGGTATTGGAAAAATAAATATGACAGGG
ACATCCTTGAGCCGTATATG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MNSSSSSVFPLFDVLVVGAGHAGIEAALAASRLGCSVAVLTHNLDTIGQMSCNPAIGGLAKGHIVREIDAMGGAMGLNTD
ATAIQFRMLNASKGPSVRAPRAQCDKTAYRTRMKMVLETAPGIRLFQGDVVKLLVNGEQQATGAVTSLGCAIHARSVIIC
SGTFMKGLLHVGLQHITGGRMGCASSSLSADLAMLGFQVERLKTGTSPRVNGRSIDFSVCEIQPGDAPEALFSYRSRDIL
KRDSEPFCTLNTWGDENFRMKQMPCWITYTNQTTHDIIRKNLKFSPLYSGIIEGVGPRYCPSIEDKIVRFAEKNSHQVFL
EPEGRSTEEFYLNGVSTSLPYSAQCDFIHTIPGLENAQLIRPGYAVEYDFCPPTQLHPTLETKLVENLYFAGQINGTSGY
EEAAAQGLMAGANAALKTLGKPSLVLRRDQAYIGVMIDDLVTKGTDEPYRMFTSRAEFRLLLRQDNADLRLAPLAFEAGL
ITCEMARKAEDRKNAVEEGIIKAAALKHEGVSLSTWLKRPGNDWTLLPEELKKMFHVEHLDAIATDIQYEGHIERQKKQA
EKIIRMENKTIPSDIDYKHVHALKKEAVIKLGNIRPATIGQASRIPGITPADIALLLVHLQKRSRQQT

Sequences:

>Translated_628_residues
MNSSSSSVFPLFDVLVVGAGHAGIEAALAASRLGCSVAVLTHNLDTIGQMSCNPAIGGLAKGHIVREIDAMGGAMGLNTD
ATAIQFRMLNASKGPSVRAPRAQCDKTAYRTRMKMVLETAPGIRLFQGDVVKLLVNGEQQATGAVTSLGCAIHARSVIIC
SGTFMKGLLHVGLQHITGGRMGCASSSLSADLAMLGFQVERLKTGTSPRVNGRSIDFSVCEIQPGDAPEALFSYRSRDIL
KRDSEPFCTLNTWGDENFRMKQMPCWITYTNQTTHDIIRKNLKFSPLYSGIIEGVGPRYCPSIEDKIVRFAEKNSHQVFL
EPEGRSTEEFYLNGVSTSLPYSAQCDFIHTIPGLENAQLIRPGYAVEYDFCPPTQLHPTLETKLVENLYFAGQINGTSGY
EEAAAQGLMAGANAALKTLGKPSLVLRRDQAYIGVMIDDLVTKGTDEPYRMFTSRAEFRLLLRQDNADLRLAPLAFEAGL
ITCEMARKAEDRKNAVEEGIIKAAALKHEGVSLSTWLKRPGNDWTLLPEELKKMFHVEHLDAIATDIQYEGHIERQKKQA
EKIIRMENKTIPSDIDYKHVHALKKEAVIKLGNIRPATIGQASRIPGITPADIALLLVHLQKRSRQQT
>Mature_628_residues
MNSSSSSVFPLFDVLVVGAGHAGIEAALAASRLGCSVAVLTHNLDTIGQMSCNPAIGGLAKGHIVREIDAMGGAMGLNTD
ATAIQFRMLNASKGPSVRAPRAQCDKTAYRTRMKMVLETAPGIRLFQGDVVKLLVNGEQQATGAVTSLGCAIHARSVIIC
SGTFMKGLLHVGLQHITGGRMGCASSSLSADLAMLGFQVERLKTGTSPRVNGRSIDFSVCEIQPGDAPEALFSYRSRDIL
KRDSEPFCTLNTWGDENFRMKQMPCWITYTNQTTHDIIRKNLKFSPLYSGIIEGVGPRYCPSIEDKIVRFAEKNSHQVFL
EPEGRSTEEFYLNGVSTSLPYSAQCDFIHTIPGLENAQLIRPGYAVEYDFCPPTQLHPTLETKLVENLYFAGQINGTSGY
EEAAAQGLMAGANAALKTLGKPSLVLRRDQAYIGVMIDDLVTKGTDEPYRMFTSRAEFRLLLRQDNADLRLAPLAFEAGL
ITCEMARKAEDRKNAVEEGIIKAAALKHEGVSLSTWLKRPGNDWTLLPEELKKMFHVEHLDAIATDIQYEGHIERQKKQA
EKIIRMENKTIPSDIDYKHVHALKKEAVIKLGNIRPATIGQASRIPGITPADIALLLVHLQKRSRQQT

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=670, Percent_Identity=40, Blast_Score=441, Evalue=1e-123,
Organism=Homo sapiens, GI19882217, Length=695, Percent_Identity=38.5611510791367, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI183227703, Length=710, Percent_Identity=37.7464788732394, Blast_Score=421, Evalue=1e-117,
Organism=Escherichia coli, GI2367273, Length=636, Percent_Identity=48.2704402515723, Blast_Score=595, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI17534255, Length=632, Percent_Identity=37.8164556962025, Blast_Score=395, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321202, Length=648, Percent_Identity=40.2777777777778, Blast_Score=442, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24658174, Length=653, Percent_Identity=40.1225114854518, Blast_Score=440, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 68855; Mature: 68855

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSSSSVFPLFDVLVVGAGHAGIEAALAASRLGCSVAVLTHNLDTIGQMSCNPAIGGLA
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCCCC
KGHIVREIDAMGGAMGLNTDATAIQFRMLNASKGPSVRAPRAQCDKTAYRTRMKMVLETA
CCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PGIRLFQGDVVKLLVNGEQQATGAVTSLGCAIHARSVIICSGTFMKGLLHVGLQHITGGR
CCCEEECCCEEEEEECCCCHHCCHHHHHHHHEEECEEEEECCHHHHHHHHHHHHHHCCCC
MGCASSSLSADLAMLGFQVERLKTGTSPRVNGRSIDFSVCEIQPGDAPEALFSYRSRDIL
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
KRDSEPFCTLNTWGDENFRMKQMPCWITYTNQTTHDIIRKNLKFSPLYSGIIEGVGPRYC
HCCCCCEEEEECCCCCCCEEECCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHCCCCCCC
PSIEDKIVRFAEKNSHQVFLEPEGRSTEEFYLNGVSTSLPYSAQCDFIHTIPGLENAQLI
CCHHHHHHHHHCCCCCEEEECCCCCCCHHEEEECCCCCCCCCCCCCEEEECCCCCCCEEE
RPGYAVEYDFCPPTQLHPTLETKLVENLYFAGQINGTSGYEEAAAQGLMAGANAALKTLG
CCCCEEEECCCCCCCCCCHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHCC
KPSLVLRRDQAYIGVMIDDLVTKGTDEPYRMFTSRAEFRLLLRQDNADLRLAPLAFEAGL
CCCEEEEECCCEEEEEHHHHHHCCCCCHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCE
ITCEMARKAEDRKNAVEEGIIKAAALKHEGVSLSTWLKRPGNDWTLLPEELKKMFHVEHL
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEECHHHHHHHHHHHHH
DAIATDIQYEGHIERQKKQAEKIIRMENKTIPSDIDYKHVHALKKEAVIKLGNIRPATIG
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCC
QASRIPGITPADIALLLVHLQKRSRQQT
CHHCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSSSSSVFPLFDVLVVGAGHAGIEAALAASRLGCSVAVLTHNLDTIGQMSCNPAIGGLA
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCCCC
KGHIVREIDAMGGAMGLNTDATAIQFRMLNASKGPSVRAPRAQCDKTAYRTRMKMVLETA
CCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PGIRLFQGDVVKLLVNGEQQATGAVTSLGCAIHARSVIICSGTFMKGLLHVGLQHITGGR
CCCEEECCCEEEEEECCCCHHCCHHHHHHHHEEECEEEEECCHHHHHHHHHHHHHHCCCC
MGCASSSLSADLAMLGFQVERLKTGTSPRVNGRSIDFSVCEIQPGDAPEALFSYRSRDIL
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
KRDSEPFCTLNTWGDENFRMKQMPCWITYTNQTTHDIIRKNLKFSPLYSGIIEGVGPRYC
HCCCCCEEEEECCCCCCCEEECCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHCCCCCCC
PSIEDKIVRFAEKNSHQVFLEPEGRSTEEFYLNGVSTSLPYSAQCDFIHTIPGLENAQLI
CCHHHHHHHHHCCCCCEEEECCCCCCCHHEEEECCCCCCCCCCCCCEEEECCCCCCCEEE
RPGYAVEYDFCPPTQLHPTLETKLVENLYFAGQINGTSGYEEAAAQGLMAGANAALKTLG
CCCCEEEECCCCCCCCCCHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHCC
KPSLVLRRDQAYIGVMIDDLVTKGTDEPYRMFTSRAEFRLLLRQDNADLRLAPLAFEAGL
CCCEEEEECCCEEEEEHHHHHHCCCCCHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCE
ITCEMARKAEDRKNAVEEGIIKAAALKHEGVSLSTWLKRPGNDWTLLPEELKKMFHVEHL
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEECHHHHHHHHHHHHH
DAIATDIQYEGHIERQKKQAEKIIRMENKTIPSDIDYKHVHALKKEAVIKLGNIRPATIG
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCC
QASRIPGITPADIALLLVHLQKRSRQQT
CHHCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA