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The map label for this gene is hisF

Identifier: 187735306

GI number: 187735306

Start: 944278

End: 945045

Strand: Reverse

Name: hisF

Synonym: Amuc_0804

Alternate gene names: 187735306

Gene position: 945045-944278 (Counterclockwise)

Preceding gene: 187735310

Following gene: 187735305

Centisome position: 35.47

GC content: 58.46

Gene sequence:

>768_bases
GTGCTGGCTAAAAGAATCATTCCGTGTCTGGACGTAACGGACGGCAGGGTCGTCAAGGGGACCAATTTCATTAATCTGAG
AGATGCCGGGGATCCTGTAGAATGTGCCAGGGCGTATGATGCCCAGCAGGCGGATGAACTTGTTTTTCTGGATATTACCG
CTTCTTCCGATGGCCGTGCCACCATGGCGGACGTGGTGCGCCGCACGGCGGCATGCTGCTTCATGCCGCTGACGGTAGGC
GGCGGCATCCGTTCCGTAAAGGACATGCGCGAAATGCTTCTGGCGGGTGCGGACAAGGTTTCCCTGAATACGGCGGCCAT
CAATAGGCCGGAGCTGATTAATGAAGGTGCGGTGGCTTTTGGCAGCCAGTGCATCGTCGTCGCTATTGACGCCAAGCGCC
AGGCTTCCGGCAAGTGGGGCGTTTCCACCCATGGAGGCCGGAAATTCGTGGGGCTGGATGCCGTGGAATGGGCTGTGGAG
GCGGAACGCCGCGGAGCCGGGGAAATCTTGTTGACCAGCATGGATGCGGACGGAGCCAAAACGGGTTATGATATTGAATT
GACCCGCGCCGTAAGCAGTGCGGTGCGCATTCCGGTAATCGCCAGCGGGGGTGCGGGTAATTTGGATCACATGGTGGACG
TGCTGGTGGAAGGGAAGGCGGATGCCGTGCTGGCGGCTTCCATTTTCCATTTTGGGGAATACACGGTGCCGGAAGCCAAG
GCTTACTTTGCTTCCAGAGGAATACCCGTGCGTCCTTTGGCGGAATAG

Upstream 100 bases:

>100_bases
AGGGCCTTGGTCAAAAAGGGAACATGTTTATCAAAAGGCATCCCGCGGCAAGCCGTATTTTAGGCTTTTCCTTTGGCGGC
GGTGATGGCTTGATAGGGCC

Downstream 100 bases:

>100_bases
CGGAAACAGCTTTTCTTTTGAGAGAAAATGAAATTCCGGTGCGTAATATGCGTATGCGCTGTCTTTTTTTTCCTCTGTGT
TCCGCTATTGCCTTAACGTC

Product: imidazoleglycerol phosphate synthase, cyclase subunit

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLAKRIIPCLDVTDGRVVKGTNFINLRDAGDPVECARAYDAQQADELVFLDITASSDGRATMADVVRRTAACCFMPLTVG
GGIRSVKDMREMLLAGADKVSLNTAAINRPELINEGAVAFGSQCIVVAIDAKRQASGKWGVSTHGGRKFVGLDAVEWAVE
AERRGAGEILLTSMDADGAKTGYDIELTRAVSSAVRIPVIASGGAGNLDHMVDVLVEGKADAVLAASIFHFGEYTVPEAK
AYFASRGIPVRPLAE

Sequences:

>Translated_255_residues
MLAKRIIPCLDVTDGRVVKGTNFINLRDAGDPVECARAYDAQQADELVFLDITASSDGRATMADVVRRTAACCFMPLTVG
GGIRSVKDMREMLLAGADKVSLNTAAINRPELINEGAVAFGSQCIVVAIDAKRQASGKWGVSTHGGRKFVGLDAVEWAVE
AERRGAGEILLTSMDADGAKTGYDIELTRAVSSAVRIPVIASGGAGNLDHMVDVLVEGKADAVLAASIFHFGEYTVPEAK
AYFASRGIPVRPLAE
>Mature_255_residues
MLAKRIIPCLDVTDGRVVKGTNFINLRDAGDPVECARAYDAQQADELVFLDITASSDGRATMADVVRRTAACCFMPLTVG
GGIRSVKDMREMLLAGADKVSLNTAAINRPELINEGAVAFGSQCIVVAIDAKRQASGKWGVSTHGGRKFVGLDAVEWAVE
AERRGAGEILLTSMDADGAKTGYDIELTRAVSSAVRIPVIASGGAGNLDHMVDVLVEGKADAVLAASIFHFGEYTVPEAK
AYFASRGIPVRPLAE

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=45.3125, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI87082028, Length=247, Percent_Identity=28.7449392712551, Blast_Score=86, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=35.7827476038339, Blast_Score=158, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_AKKM8 (B2UQA2)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877418.1
- ProteinModelPortal:   B2UQA2
- SMR:   B2UQA2
- GeneID:   6274373
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0804
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26912; Mature: 26912

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVTDGRVVKGTNFINLRDAGDPVECARAYDAQQADELVFLDITASSDGRA
CCHHHCCCEEECCCCEEEECCCEEEECCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCCH
TMADVVRRTAACCFMPLTVGGGIRSVKDMREMLLAGADKVSLNTAAINRPELINEGAVAF
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEHCCCCCCHHHCCCCCEE
GSQCIVVAIDAKRQASGKWGVSTHGGRKFVGLDAVEWAVEAERRGAGEILLTSMDADGAK
CCCEEEEEEECCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCC
TGYDIELTRAVSSAVRIPVIASGGAGNLDHMVDVLVEGKADAVLAASIFHFGEYTVPEAK
CCCCEEEHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHH
AYFASRGIPVRPLAE
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVTDGRVVKGTNFINLRDAGDPVECARAYDAQQADELVFLDITASSDGRA
CCHHHCCCEEECCCCEEEECCCEEEECCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCCH
TMADVVRRTAACCFMPLTVGGGIRSVKDMREMLLAGADKVSLNTAAINRPELINEGAVAF
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEHCCCCCCHHHCCCCCEE
GSQCIVVAIDAKRQASGKWGVSTHGGRKFVGLDAVEWAVEAERRGAGEILLTSMDADGAK
CCCEEEEEEECCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCC
TGYDIELTRAVSSAVRIPVIASGGAGNLDHMVDVLVEGKADAVLAASIFHFGEYTVPEAK
CCCCEEEHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHH
AYFASRGIPVRPLAE
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA