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The map label for this gene is mdeA [H]

Identifier: 187735293

GI number: 187735293

Start: 927172

End: 928473

Strand: Reverse

Name: mdeA [H]

Synonym: Amuc_0790

Alternate gene names: 187735293

Gene position: 928473-927172 (Counterclockwise)

Preceding gene: 187735294

Following gene: 187735292

Centisome position: 34.85

GC content: 54.45

Gene sequence:

>1302_bases
ATGCCCCGCATGATGAATCAGGAATTGAGACCGGAGACCCTGTGCGTGCAGGCGGGCTGGGCGCCTAAAAAGGGCGAACC
CCGCGTATTGCCCATTTACCAGAGCACGACGTTCAAGTACGAGACCAGCGAACAAATGGCAAAATTGTTTGATCTGGAAG
AAGCCGGATTCTTTTATACACGCCTCCAGAACCCCACCAATGAGGCGGTTGCCAGAAAAATTGCCGAACTGGAGGGCGGT
GTGGCCGCCATTCTGACGTCTTCCGGCCAGGCGGCTTCCTTCTTTGCCATTTTCAATATTTGCGAGGCTGGGGATCATAT
CGTCAGTGCTTCGGCGATTTACGGGGGCACATACAACCTCTTTGCCGTCACATTCCGGAAAATGGGCATTGAAGTTACTT
TCGTGGACCAGGACGCTCCGGCGGAGGAAATCGCCAAAGCGTTCCGCCCCAATACGAAAGCTTTCTTTGCGGAGACTATT
TCCAATCCCACGCTTTGCGTGCTGGATATCCGTAAAATGGCGGATATTGCCCATGCGCACGGCGTTCCCCTCATTGTGGA
CAATACCTTTGCCACACCCATTAATTGCCGTCCTTTTGAATGGGGTGCGGATATCGTCACCCACTCCACCACCAAGTACA
TGGACGGCCATGCCGCCGCCGTGGGCGGCGTGATTGTGGACAGCGGCAACTTTGACTGGGAAGCGCACAAGGATAAATTC
CCGGGTTTGACGGAACCGGATCCCTCCTATCACGGGCTCTCCTACAGCAAAAGCTTCGGCAAGGGAGCCTATATTACCAA
GGCGACCGTCCAGCTCATGCGCGATCTGGGTTCCATCCAATCTCCGCAGAATGCCTTTTTGCTCAATTTGGGTTTGGAAA
CCCTCCACCTGCGTGTGCCGCGGCATTGTGAAAATGCGCAGAAAGTGGCGGAATTCCTGCAAGGGCAGGAAGATGTTGCC
TGGATTAATTATCCGGGTCTTTCTTCCAATGAGTATTATGAATTGGCGCAGAAGCAATTCCGCGGTGGCCAATCTTGTGG
TGTGGTAACCTTCGGCATTAAGGGAGGCCGTGAACGTTCCATCAAGTTCATGGACAGTTTGAAGCTGGCCGCCATCGTGA
CCCATGTGGCGGACTCCCGTACCTGCGTTCTCCATCCCGCCAGCCATACGCACCGCCAGTTGACGGATGAGCAGCTCATG
GAGGCGGGCGTACGGCCTGACCTCATCCGGTTTTCCGTAGGGACGGAAGCTGCGGAAGACATTATTGCGGACCTCAAACA
GGCTTTGGAAGCTATTCGTTAA

Upstream 100 bases:

>100_bases
TACTGGGAAAAAACCGGGAGTGTGCTTCGGCGGGGGCAGGGGCGAATTTGTCCATACGTCTGGTTTTCCCTGCTTTACAA
GGCAGGAGGCATCGCATAGC

Downstream 100 bases:

>100_bases
AAAGTCTTCTTGGAAAAAGCCGCCTCACTTTGCTGAGGCGGCTTTTTCTTTATCATTAAGGATTGGATAAAGGGGACAGC
GGATTGTTAATCCGTTGTCA

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MPRMMNQELRPETLCVQAGWAPKKGEPRVLPIYQSTTFKYETSEQMAKLFDLEEAGFFYTRLQNPTNEAVARKIAELEGG
VAAILTSSGQAASFFAIFNICEAGDHIVSASAIYGGTYNLFAVTFRKMGIEVTFVDQDAPAEEIAKAFRPNTKAFFAETI
SNPTLCVLDIRKMADIAHAHGVPLIVDNTFATPINCRPFEWGADIVTHSTTKYMDGHAAAVGGVIVDSGNFDWEAHKDKF
PGLTEPDPSYHGLSYSKSFGKGAYITKATVQLMRDLGSIQSPQNAFLLNLGLETLHLRVPRHCENAQKVAEFLQGQEDVA
WINYPGLSSNEYYELAQKQFRGGQSCGVVTFGIKGGRERSIKFMDSLKLAAIVTHVADSRTCVLHPASHTHRQLTDEQLM
EAGVRPDLIRFSVGTEAAEDIIADLKQALEAIR

Sequences:

>Translated_433_residues
MPRMMNQELRPETLCVQAGWAPKKGEPRVLPIYQSTTFKYETSEQMAKLFDLEEAGFFYTRLQNPTNEAVARKIAELEGG
VAAILTSSGQAASFFAIFNICEAGDHIVSASAIYGGTYNLFAVTFRKMGIEVTFVDQDAPAEEIAKAFRPNTKAFFAETI
SNPTLCVLDIRKMADIAHAHGVPLIVDNTFATPINCRPFEWGADIVTHSTTKYMDGHAAAVGGVIVDSGNFDWEAHKDKF
PGLTEPDPSYHGLSYSKSFGKGAYITKATVQLMRDLGSIQSPQNAFLLNLGLETLHLRVPRHCENAQKVAEFLQGQEDVA
WINYPGLSSNEYYELAQKQFRGGQSCGVVTFGIKGGRERSIKFMDSLKLAAIVTHVADSRTCVLHPASHTHRQLTDEQLM
EAGVRPDLIRFSVGTEAAEDIIADLKQALEAIR
>Mature_432_residues
PRMMNQELRPETLCVQAGWAPKKGEPRVLPIYQSTTFKYETSEQMAKLFDLEEAGFFYTRLQNPTNEAVARKIAELEGGV
AAILTSSGQAASFFAIFNICEAGDHIVSASAIYGGTYNLFAVTFRKMGIEVTFVDQDAPAEEIAKAFRPNTKAFFAETIS
NPTLCVLDIRKMADIAHAHGVPLIVDNTFATPINCRPFEWGADIVTHSTTKYMDGHAAAVGGVIVDSGNFDWEAHKDKFP
GLTEPDPSYHGLSYSKSFGKGAYITKATVQLMRDLGSIQSPQNAFLLNLGLETLHLRVPRHCENAQKVAEFLQGQEDVAW
INYPGLSSNEYYELAQKQFRGGQSCGVVTFGIKGGRERSIKFMDSLKLAAIVTHVADSRTCVLHPASHTHRQLTDEQLME
AGVRPDLIRFSVGTEAAEDIIADLKQALEAIR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=422, Percent_Identity=33.8862559241706, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI299473759, Length=422, Percent_Identity=31.5165876777251, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI25453487, Length=421, Percent_Identity=29.2161520190024, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI1790375, Length=404, Percent_Identity=30.9405940594059, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1789383, Length=389, Percent_Identity=25.1928020565553, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71980708, Length=412, Percent_Identity=35.6796116504854, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17560056, Length=424, Percent_Identity=32.7830188679245, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17538055, Length=424, Percent_Identity=32.311320754717, Blast_Score=186, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=416, Percent_Identity=45.4326923076923, Blast_Score=340, Evalue=3e-94,
Organism=Saccharomyces cerevisiae, GI6319307, Length=405, Percent_Identity=34.8148148148148, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321170, Length=331, Percent_Identity=26.2839879154079, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321254, Length=446, Percent_Identity=25.3363228699552, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321904, Length=416, Percent_Identity=27.1634615384615, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922554, Length=434, Percent_Identity=31.3364055299539, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47677; Mature: 47545

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRMMNQELRPETLCVQAGWAPKKGEPRVLPIYQSTTFKYETSEQMAKLFDLEEAGFFYT
CCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEECCHHHHHHHHHHHHCCEEEE
RLQNPTNEAVARKIAELEGGVAAILTSSGQAASFFAIFNICEAGDHIVSASAIYGGTYNL
ECCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEECCCHHH
FAVTFRKMGIEVTFVDQDAPAEEIAKAFRPNTKAFFAETISNPTLCVLDIRKMADIAHAH
HHHHHHHHCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHC
GVPLIVDNTFATPINCRPFEWGADIVTHSTTKYMDGHAAAVGGVIVDSGNFDWEAHKDKF
CCCEEEECCCCCCCCCCCCCCCCHHHCCCCHHHCCCCHHEECCEEEECCCCCCCCCCCCC
PGLTEPDPSYHGLSYSKSFGKGAYITKATVQLMRDLGSIQSPQNAFLLNLGLETLHLRVP
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEECC
RHCENAQKVAEFLQGQEDVAWINYPGLSSNEYYELAQKQFRGGQSCGVVTFGIKGGRERS
HHCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCH
IKFMDSLKLAAIVTHVADSRTCVLHPASHTHRQLTDEQLMEAGVRPDLIRFSVGTEAAED
HHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCHHHHHHCCCCCCEEEEECCCHHHHH
IIADLKQALEAIR
HHHHHHHHHHHCC
>Mature Secondary Structure 
PRMMNQELRPETLCVQAGWAPKKGEPRVLPIYQSTTFKYETSEQMAKLFDLEEAGFFYT
CCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEECCHHHHHHHHHHHHCCEEEE
RLQNPTNEAVARKIAELEGGVAAILTSSGQAASFFAIFNICEAGDHIVSASAIYGGTYNL
ECCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEECCCHHH
FAVTFRKMGIEVTFVDQDAPAEEIAKAFRPNTKAFFAETISNPTLCVLDIRKMADIAHAH
HHHHHHHHCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHHC
GVPLIVDNTFATPINCRPFEWGADIVTHSTTKYMDGHAAAVGGVIVDSGNFDWEAHKDKF
CCCEEEECCCCCCCCCCCCCCCCHHHCCCCHHHCCCCHHEECCEEEECCCCCCCCCCCCC
PGLTEPDPSYHGLSYSKSFGKGAYITKATVQLMRDLGSIQSPQNAFLLNLGLETLHLRVP
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEECC
RHCENAQKVAEFLQGQEDVAWINYPGLSSNEYYELAQKQFRGGQSCGVVTFGIKGGRERS
HHCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCH
IKFMDSLKLAAIVTHVADSRTCVLHPASHTHRQLTDEQLMEAGVRPDLIRFSVGTEAAED
HHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCHHHHHHCCCCCCEEEEECCCHHHHH
IIADLKQALEAIR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]