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The map label for this gene is leuB [H]

Identifier: 187735288

GI number: 187735288

Start: 921168

End: 922277

Strand: Direct

Name: leuB [H]

Synonym: Amuc_0785

Alternate gene names: 187735288

Gene position: 921168-922277 (Clockwise)

Preceding gene: 187735283

Following gene: 187735289

Centisome position: 34.58

GC content: 59.01

Gene sequence:

>1110_bases
ATGAGTGAACATCACCATCATATTGCTGTCTTGGCCGGCGACGGTATCGGTCCCGAGGTCATGGCACAGGCTCTGAAGGT
TCTGGATGCCGTAAGCGGCAAGTTTGGCTTTACCGTAAGCCGCAAGGAGGCATTTGTAGGGGGTGCGGGCATTGACCACT
GCGGCAAGGCTCTTCCCGAAGAAACCATTCGCGCCTGCGAAGAGGCGGACGCCGTTCTGTTCGGCTCCGTGGGCGGTCCC
AAATGGGAGCATCTCCCCGCCAATGAACAACCGGAACGCGGAGCCCTGCTGCCTCTGAGAAAACATTTCGGCTTGTATGC
CAACCTGCGTCCGGGCGTATGCCTTCCGGCCTTGACCCATGCCTCCCCCATCAAGAATGAACTGATTGAAGGCGGATTCG
ATATTTTATGTGTCCGGGAATTGACTGGCGGCCTGTATTTCGGCCAGCCCCGTTTCCGCGAACGGGAGGGAGACGACGAA
GTCGTCGTGGATACCATGCGCTACCATAAAAGCGAGATGGTGCGTATCGCCAAAGTGGCGTTCGAGGCTGCGCGCGGCCG
CCGTAAGCGCGTCACCAGCGTGGACAAGGCCAATGTGCTGACCAATTCCCTGCTGTGGCGCGAGACAATGATTGAAGTTT
CCAAGGATTATCCCGACGTGGAATTGCTGCATATGTACGTGGACAATGCAGCCATGCAGCTGGTGCGCAATCCCCGCCAG
TTCGACGTGCTGGTGACGGAAAACCTGTTCGGGGACATTCTTTCCGATGAAATGGCCATGATTTGCGGCTCCCTGGGCAT
GCTGCCCAGCGCCAGCCTGTGCCAAGGCGCGCAGGACAACGGCCTGTTCTTCGGCCTTTACGAACCCTCCGGAGGCTCCG
CCCCGGATATTGCCGGCAAGGGCATTGCAAACCCAATCGCCCAGATTCTTTCCCTTTCCATGCTGTTGCGCTACTCCCTG
GGAGAAAAAACAGCGGCAGACGCCATTGACTCCGCCGTTCGCCGCGTGATTGACCAGGGCTGCCGCACGGGCGACCTGGC
TACGGGAGCTCCCGGAGAAATCCGCGTGAATACGGCGGAAATGGGTGACGCCATCATCGCCGCCCTGTAA

Upstream 100 bases:

>100_bases
ACTCTTGCAGGCTTTTTGACGCAAAAAGGCTTCCGCGGCGCGGTCATGGCAACGGTGTTTTCTTGACCTTTGAAGGGAGA
ATGACCAGACTTTCAGCGAC

Downstream 100 bases:

>100_bases
ACAGGGCCGTTCTTCCGGCGCCGCAGGGGAAGGGACACCTGCGGCACGCCTTTCCTCCGGGGGGAGCGGTACCGCCTCCC
CTCCGCTTTCCTCTTTCATT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSEHHHHIAVLAGDGIGPEVMAQALKVLDAVSGKFGFTVSRKEAFVGGAGIDHCGKALPEETIRACEEADAVLFGSVGGP
KWEHLPANEQPERGALLPLRKHFGLYANLRPGVCLPALTHASPIKNELIEGGFDILCVRELTGGLYFGQPRFREREGDDE
VVVDTMRYHKSEMVRIAKVAFEAARGRRKRVTSVDKANVLTNSLLWRETMIEVSKDYPDVELLHMYVDNAAMQLVRNPRQ
FDVLVTENLFGDILSDEMAMICGSLGMLPSASLCQGAQDNGLFFGLYEPSGGSAPDIAGKGIANPIAQILSLSMLLRYSL
GEKTAADAIDSAVRRVIDQGCRTGDLATGAPGEIRVNTAEMGDAIIAAL

Sequences:

>Translated_369_residues
MSEHHHHIAVLAGDGIGPEVMAQALKVLDAVSGKFGFTVSRKEAFVGGAGIDHCGKALPEETIRACEEADAVLFGSVGGP
KWEHLPANEQPERGALLPLRKHFGLYANLRPGVCLPALTHASPIKNELIEGGFDILCVRELTGGLYFGQPRFREREGDDE
VVVDTMRYHKSEMVRIAKVAFEAARGRRKRVTSVDKANVLTNSLLWRETMIEVSKDYPDVELLHMYVDNAAMQLVRNPRQ
FDVLVTENLFGDILSDEMAMICGSLGMLPSASLCQGAQDNGLFFGLYEPSGGSAPDIAGKGIANPIAQILSLSMLLRYSL
GEKTAADAIDSAVRRVIDQGCRTGDLATGAPGEIRVNTAEMGDAIIAAL
>Mature_368_residues
SEHHHHIAVLAGDGIGPEVMAQALKVLDAVSGKFGFTVSRKEAFVGGAGIDHCGKALPEETIRACEEADAVLFGSVGGPK
WEHLPANEQPERGALLPLRKHFGLYANLRPGVCLPALTHASPIKNELIEGGFDILCVRELTGGLYFGQPRFREREGDDEV
VVDTMRYHKSEMVRIAKVAFEAARGRRKRVTSVDKANVLTNSLLWRETMIEVSKDYPDVELLHMYVDNAAMQLVRNPRQF
DVLVTENLFGDILSDEMAMICGSLGMLPSASLCQGAQDNGLFFGLYEPSGGSAPDIAGKGIANPIAQILSLSMLLRYSLG
EKTAADAIDSAVRRVIDQGCRTGDLATGAPGEIRVNTAEMGDAIIAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=335, Percent_Identity=34.9253731343284, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI28178816, Length=367, Percent_Identity=27.7929155313352, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI4758582, Length=341, Percent_Identity=30.4985337243402, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178838, Length=341, Percent_Identity=30.4985337243402, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178821, Length=344, Percent_Identity=27.906976744186, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI28178819, Length=176, Percent_Identity=35.2272727272727, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI87081683, Length=361, Percent_Identity=62.8808864265928, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1788101, Length=367, Percent_Identity=35.149863760218, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1787381, Length=406, Percent_Identity=25.615763546798, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=358, Percent_Identity=32.4022346368715, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=346, Percent_Identity=30.3468208092486, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=346, Percent_Identity=30.3468208092486, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=366, Percent_Identity=29.5081967213115, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319830, Length=368, Percent_Identity=44.5652173913043, Blast_Score=262, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6322097, Length=374, Percent_Identity=34.4919786096257, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324709, Length=377, Percent_Identity=31.2997347480106, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324291, Length=341, Percent_Identity=30.4985337243402, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24643268, Length=371, Percent_Identity=32.0754716981132, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24643270, Length=371, Percent_Identity=32.0754716981132, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24661184, Length=371, Percent_Identity=30.7277628032345, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI161078635, Length=352, Percent_Identity=30.3977272727273, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078633, Length=352, Percent_Identity=30.3977272727273, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24650122, Length=352, Percent_Identity=30.3977272727273, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078637, Length=352, Percent_Identity=30.3977272727273, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078639, Length=348, Percent_Identity=30.4597701149425, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281362242, Length=343, Percent_Identity=28.8629737609329, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648872, Length=343, Percent_Identity=28.8629737609329, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20130355, Length=347, Percent_Identity=25.0720461095101, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39798; Mature: 39667

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHHHHIAVLAGDGIGPEVMAQALKVLDAVSGKFGFTVSRKEAFVGGAGIDHCGKALPE
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHEECCCCHHHHHHCCCH
ETIRACEEADAVLFGSVGGPKWEHLPANEQPERGALLPLRKHFGLYANLRPGVCLPALTH
HHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCCHHHHHC
ASPIKNELIEGGFDILCVRELTGGLYFGQPRFREREGDDEVVVDTMRYHKSEMVRIAKVA
CCHHHHHHHHCCCCEEEEEHHHCCEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
FEAARGRRKRVTSVDKANVLTNSLLWRETMIEVSKDYPDVELLHMYVDNAAMQLVRNPRQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
FDVLVTENLFGDILSDEMAMICGSLGMLPSASLCQGAQDNGLFFGLYEPSGGSAPDIAGK
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCC
GIANPIAQILSLSMLLRYSLGEKTAADAIDSAVRRVIDQGCRTGDLATGAPGEIRVNTAE
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHH
MGDAIIAAL
HCCCHHHCC
>Mature Secondary Structure 
SEHHHHIAVLAGDGIGPEVMAQALKVLDAVSGKFGFTVSRKEAFVGGAGIDHCGKALPE
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHEECCCCHHHHHHCCCH
ETIRACEEADAVLFGSVGGPKWEHLPANEQPERGALLPLRKHFGLYANLRPGVCLPALTH
HHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCCHHHHHC
ASPIKNELIEGGFDILCVRELTGGLYFGQPRFREREGDDEVVVDTMRYHKSEMVRIAKVA
CCHHHHHHHHCCCCEEEEEHHHCCEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
FEAARGRRKRVTSVDKANVLTNSLLWRETMIEVSKDYPDVELLHMYVDNAAMQLVRNPRQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
FDVLVTENLFGDILSDEMAMICGSLGMLPSASLCQGAQDNGLFFGLYEPSGGSAPDIAGK
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCC
GIANPIAQILSLSMLLRYSLGEKTAADAIDSAVRRVIDQGCRTGDLATGAPGEIRVNTAE
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHH
MGDAIIAAL
HCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA