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The map label for this gene is murE1 [H]

Identifier: 187735159

GI number: 187735159

Start: 767420

End: 768925

Strand: Direct

Name: murE1 [H]

Synonym: Amuc_0653

Alternate gene names: 187735159

Gene position: 767420-768925 (Clockwise)

Preceding gene: 187735158

Following gene: 187735160

Centisome position: 28.81

GC content: 61.09

Gene sequence:

>1506_bases
ATGAAGCTACTTACCTTACTCAAAGACCTTCCCTCCCATACGCTCACCGGCTCCCCCCAGGTGAAAATCTCCCATCTGGA
ATGCGACAGCCGGCGCATTCTGCCCGGTTCCTGCTACATTGCCCTGAAGGGAACCCGGGCGGACGGCCATGAATTCATCC
CGGAGGCCATTCGCCGCGGAGCCTGCGCCGTCGTCGCGGAAATGCCCTGCACGCAGGAAGCCAGGGAAGCCGGAGTCAGC
TGGGTGGAGGTGAACGACTCCCGCCTGGCCGTCAGCCTGATGGGCGCGGCCTGGAACGGACGCCCTTCCCGCCACATGAC
CATGATCGGCGTGACGGGGACGAACGGAAAAACCACAACGGCCTACATCGCCCATTCCCTGCTCAAGCAGTCCTGGCTGC
GCGCTGGATTGATCGGCACCATCGCCTATGACAACGGGGAGGAAATCACTCCTTCCACCCATACCACGCCCGGACCGCTG
GAACTGGAACACCTGCTGAAGGAAATGTATGAAAACGGCTGCCGCGGCGTCTCCATGGAGGTCTCTTCCCACGCGCTGGA
CCAGGAACGCGTGGCCGGCATCAATTTCAACGTGGGCATTTTCACCAATCTGACCCAGGACCACCTGGACTACCATCACA
CCATGGAGAATTATTTCCAGGCCAAGGCGAAATTGTTCGAACAGATGGCACAGGACACCAAATCCCGGCGAAAACCCGTG
GCCGTCATCAACATCGACGACGCTTACGGCCGCCGACTGGCGGAAATGTTTTCCGGCCGCATGACCGTAAAGACCTACGG
GTCCGCCCTGGGGGCGGATTTCCGCATGCTGGTGCACCACGCCACCGCCAAAGGCAGCGAGTACGAATTGGAATACAAGG
GGAAAAGCTACCTGGTCCGTGTCCCCCTGATCGGCAAGTTCAACATGTACAACAGCCTGGCCGCGCTGGCCGCCGTCATT
TGCGCCGGCATCCCCGTGCGGGACGCCATTGCGAACCTTCAGAATATTCCGCAGGTCCCCGGCAGGCTGGAACTGTTCAC
CCACCCCGGAGGCGCCCAGATTTTCATTGACTACGCCCACACGCCGGACGCCCTGGAAAACGTCTGCAAGACGCTCAAGG
AACTGTGCTCCCGCCGCCTGATTACCGTGTTCGGCTGCGGCGGGGACCGGGACAGGGGAAAACGCCCCCTGATGGGAGCC
GCTGCCGCGCGCCTGTCAGACGCCTGCATCGTCACCTCCGACAATCCCAGAAGCGAGGAACCCCTCTCCATCATCAGCCA
GATTGCGGCGGGCATGCCGGAAGGCAGATACGCCATCATCCCGGACCGGGCCAGAGCCATTGCCACGGCCATCGAGCAGG
CCCGTCTGGGGGACATCGTCCTCATTGCGGGCAAGGGCCATGAAAATTACCAGGAACTTTCCACCGGCCGCATTGATTTC
AGCGATGCCAAGGAAGTGCGCCGCAACATGTTCATCAAGGAACGGGAAGAATTCCCCCAGGCATAA

Upstream 100 bases:

>100_bases
AACATTCCTAAGAACGTCCCTTCCGCAGAGGCAAATAAAAAAGCCTCCCGTGCCCAGGCCTCTTCCACCCCATCCAAACC
GCCGCGCAGATAATCCCGTC

Downstream 100 bases:

>100_bases
CACGTTATTCCGTCTTTCTTTCATGATTTCCCTCACGGCACACGGCATCAGCAACGTCATCGGCGGAAAACTGGTCAGCG
GCCCCTTCGACAGGGTCGCT

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme 1; Meso-diaminopimelate-adding enzyme 1; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase 1; UDP-MurNAc-tripeptide synthetase 1; UDP-N-acetylmuramyl-tripeptide synthetase 1 [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MKLLTLLKDLPSHTLTGSPQVKISHLECDSRRILPGSCYIALKGTRADGHEFIPEAIRRGACAVVAEMPCTQEAREAGVS
WVEVNDSRLAVSLMGAAWNGRPSRHMTMIGVTGTNGKTTTAYIAHSLLKQSWLRAGLIGTIAYDNGEEITPSTHTTPGPL
ELEHLLKEMYENGCRGVSMEVSSHALDQERVAGINFNVGIFTNLTQDHLDYHHTMENYFQAKAKLFEQMAQDTKSRRKPV
AVINIDDAYGRRLAEMFSGRMTVKTYGSALGADFRMLVHHATAKGSEYELEYKGKSYLVRVPLIGKFNMYNSLAALAAVI
CAGIPVRDAIANLQNIPQVPGRLELFTHPGGAQIFIDYAHTPDALENVCKTLKELCSRRLITVFGCGGDRDRGKRPLMGA
AAARLSDACIVTSDNPRSEEPLSIISQIAAGMPEGRYAIIPDRARAIATAIEQARLGDIVLIAGKGHENYQELSTGRIDF
SDAKEVRRNMFIKEREEFPQA

Sequences:

>Translated_501_residues
MKLLTLLKDLPSHTLTGSPQVKISHLECDSRRILPGSCYIALKGTRADGHEFIPEAIRRGACAVVAEMPCTQEAREAGVS
WVEVNDSRLAVSLMGAAWNGRPSRHMTMIGVTGTNGKTTTAYIAHSLLKQSWLRAGLIGTIAYDNGEEITPSTHTTPGPL
ELEHLLKEMYENGCRGVSMEVSSHALDQERVAGINFNVGIFTNLTQDHLDYHHTMENYFQAKAKLFEQMAQDTKSRRKPV
AVINIDDAYGRRLAEMFSGRMTVKTYGSALGADFRMLVHHATAKGSEYELEYKGKSYLVRVPLIGKFNMYNSLAALAAVI
CAGIPVRDAIANLQNIPQVPGRLELFTHPGGAQIFIDYAHTPDALENVCKTLKELCSRRLITVFGCGGDRDRGKRPLMGA
AAARLSDACIVTSDNPRSEEPLSIISQIAAGMPEGRYAIIPDRARAIATAIEQARLGDIVLIAGKGHENYQELSTGRIDF
SDAKEVRRNMFIKEREEFPQA
>Mature_501_residues
MKLLTLLKDLPSHTLTGSPQVKISHLECDSRRILPGSCYIALKGTRADGHEFIPEAIRRGACAVVAEMPCTQEAREAGVS
WVEVNDSRLAVSLMGAAWNGRPSRHMTMIGVTGTNGKTTTAYIAHSLLKQSWLRAGLIGTIAYDNGEEITPSTHTTPGPL
ELEHLLKEMYENGCRGVSMEVSSHALDQERVAGINFNVGIFTNLTQDHLDYHHTMENYFQAKAKLFEQMAQDTKSRRKPV
AVINIDDAYGRRLAEMFSGRMTVKTYGSALGADFRMLVHHATAKGSEYELEYKGKSYLVRVPLIGKFNMYNSLAALAAVI
CAGIPVRDAIANLQNIPQVPGRLELFTHPGGAQIFIDYAHTPDALENVCKTLKELCSRRLITVFGCGGDRDRGKRPLMGA
AAARLSDACIVTSDNPRSEEPLSIISQIAAGMPEGRYAIIPDRARAIATAIEQARLGDIVLIAGKGHENYQELSTGRIDF
SDAKEVRRNMFIKEREEFPQA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=491, Percent_Identity=36.2525458248472, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1786274, Length=384, Percent_Identity=25, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 55071; Mature: 55071

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLTLLKDLPSHTLTGSPQVKISHLECDSRRILPGSCYIALKGTRADGHEFIPEAIRRG
CHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEECCCEEEEEEECCCCCCHHHHHHHHHCC
ACAVVAEMPCTQEAREAGVSWVEVNDSRLAVSLMGAAWNGRPSRHMTMIGVTGTNGKTTT
CEEEEECCCCCHHHHHCCCEEEEECCCEEEEEEECCCCCCCCCCEEEEEEEECCCCCCHH
AYIAHSLLKQSWLRAGLIGTIAYDNGEEITPSTHTTPGPLELEHLLKEMYENGCRGVSME
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
VSSHALDQERVAGINFNVGIFTNLTQDHLDYHHTMENYFQAKAKLFEQMAQDTKSRRKPV
HHHHHCCHHHHCCEEECEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AVINIDDAYGRRLAEMFSGRMTVKTYGSALGADFRMLVHHATAKGSEYELEYKGKSYLVR
EEEECCHHHHHHHHHHHCCCEEEEEHHHHHCCHHHHHHHHHHCCCCCEEEEECCCEEEEE
VPLIGKFNMYNSLAALAAVICAGIPVRDAIANLQNIPQVPGRLELFTHPGGAQIFIDYAH
ECCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECC
TPDALENVCKTLKELCSRRLITVFGCGGDRDRGKRPLMGAAAARLSDACIVTSDNPRSEE
CCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCC
PLSIISQIAAGMPEGRYAIIPDRARAIATAIEQARLGDIVLIAGKGHENYQELSTGRIDF
HHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCC
SDAKEVRRNMFIKEREEFPQA
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLLTLLKDLPSHTLTGSPQVKISHLECDSRRILPGSCYIALKGTRADGHEFIPEAIRRG
CHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEECCCEEEEEEECCCCCCHHHHHHHHHCC
ACAVVAEMPCTQEAREAGVSWVEVNDSRLAVSLMGAAWNGRPSRHMTMIGVTGTNGKTTT
CEEEEECCCCCHHHHHCCCEEEEECCCEEEEEEECCCCCCCCCCEEEEEEEECCCCCCHH
AYIAHSLLKQSWLRAGLIGTIAYDNGEEITPSTHTTPGPLELEHLLKEMYENGCRGVSME
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
VSSHALDQERVAGINFNVGIFTNLTQDHLDYHHTMENYFQAKAKLFEQMAQDTKSRRKPV
HHHHHCCHHHHCCEEECEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
AVINIDDAYGRRLAEMFSGRMTVKTYGSALGADFRMLVHHATAKGSEYELEYKGKSYLVR
EEEECCHHHHHHHHHHHCCCEEEEEHHHHHCCHHHHHHHHHHCCCCCEEEEECCCEEEEE
VPLIGKFNMYNSLAALAAVICAGIPVRDAIANLQNIPQVPGRLELFTHPGGAQIFIDYAH
ECCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECC
TPDALENVCKTLKELCSRRLITVFGCGGDRDRGKRPLMGAAAARLSDACIVTSDNPRSEE
CCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCC
PLSIISQIAAGMPEGRYAIIPDRARAIATAIEQARLGDIVLIAGKGHENYQELSTGRIDF
HHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCC
SDAKEVRRNMFIKEREEFPQA
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]