Click here to switch to the map view.

The map label for this gene is hybA

Identifier: 187732487

GI number: 187732487

Start: 3186045

End: 3187031

Strand: Reverse

Name: hybA

Synonym: SbBS512_E3423

Alternate gene names: 187732487

Gene position: 3187031-3186045 (Counterclockwise)

Preceding gene: 187731765

Following gene: 187732960

Centisome position: 69.04

GC content: 54.61

Gene sequence:

>987_bases
GTGAACAGACGTAATTTTATTAAAGCAGCCTCCTGCGGGGCATTGCTGACGGGCGCGCTGCCGTCTGTCAGTCATGCGGC
TGCTGAAAACCACCCGCCAATTCCGGGATCGCTGGGGATGTTGTACGACTCGACCTTGTGCGTAGGCTGCCAGGCTTGCG
TCACCAAGTGTCAGGATATCAACTTCCCTGAACGTAACCCGCAAGGGGAACAGACCTGGTCGAACAACGACAAACTGTCG
CCGTATACCAATAACATCATTCAGGTGTGGACCAGCGGCACAGGGGTCAACAAAGACCAGGAGGAGAACGGCTACGCGTA
CATTAAGAAACAGTGTATGCACTGCGTCGATCCGAACTGTGTCTCTGTGTGCCCGGTCTCTGCACTGAAAAAAGATCCGA
AAACCGGCATTGTCCATTACGACAAAGACGTGTGCACCGGTTGCCGTTACTGCATGGTCGCCTGTCCGTACAACGTGCCG
AAGTACGACTACAACAACCCGTTTGGTGCGCTGCATAAGTGCGAGCTGTGCAACCAGAAAGGTGTGGAACGTCTCGATAA
AGGCGGTCTGCCTGGCTGCGTAGAAGTGTGCCCGGCGGGCGCGGTGATTTTCGGTACGCGTGAAGAGCTGATGGCGGAGG
CGAAAAAACGTCTGGCGCTGAAGCCTGGCAGCGAATACCACTATCCGCGTCAGACGCTGAAATCTGGCGACACTTACCTG
CATACGGTGCCGAAATATTATCCGCATCTGTACGGCGAGAAAGAGGGCGGCGGTACTCAGGTTCTGGTACTGACGGGTGT
GCCTTATGAAAATCTCGACCTGCCGAAACTGGACGATCTTTCTACCGGTGCGCGTTCCGAAAATATTCAACACACCCTGT
ATAAAGGCATGATGCTACCACTGGCTGTGCTGGCGGGCTTAACCGTGCTGGTTCGTCGCAACACCAAAAACGACCATCAC
GACGGAGGAGACGATCATGAGTCATGA

Upstream 100 bases:

>100_bases
GCGGTGTGGTTGGGCTGGTTGCCGGTGTCAGCGTGATGGCGGTGCGTGAACTGGGTCGTCAGCAAAAGAAAGATAACGCT
GACTCACGGGGAGAATAACC

Downstream 100 bases:

>100_bases
TCCACAACCGCTGGGCGGCAAAATCATCAGTAAACCGGTCATGATTTTTGGACCGTTAATCGTCATCTGTATGCTCCTGA
TTGTGAAGCGTCTGGTGTTC

Product: hydrogenase 2 protein HybA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNRRNFIKAASCGALLTGALPSVSHAAAENHPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLS
PYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHH
DGGDDHES

Sequences:

>Translated_328_residues
MNRRNFIKAASCGALLTGALPSVSHAAAENHPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLS
PYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHH
DGGDDHES
>Mature_328_residues
MNRRNFIKAASCGALLTGALPSVSHAAAENHPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLS
PYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHH
DGGDDHES

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=328, Percent_Identity=99.6951219512195, Blast_Score=679, Evalue=0.0,
Organism=Escherichia coli, GI2367345, Length=255, Percent_Identity=30.5882352941176, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787749, Length=283, Percent_Identity=27.5618374558304, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790326, Length=184, Percent_Identity=33.1521739130435, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787872, Length=198, Percent_Identity=34.3434343434343, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787122, Length=198, Percent_Identity=33.8383838383838, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI226510944, Length=209, Percent_Identity=30.622009569378, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787478, Length=133, Percent_Identity=33.8345864661654, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787740, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082180, Length=192, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 35984; Mature: 35984

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRNFIKAASCGALLTGALPSVSHAAAENHPPIPGSLGMLYDSTLCVGCQACVTKCQDI
CCCCHHHHHHHCCHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNC
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVPKYDYNNPFGALHKCELCNQK
HHCCCHHHHHCCCCCCCEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
GVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HHHHHHCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEE
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLP
EHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LAVLAGLTVLVRRNTKNDHHDGGDDHES
HHHHHHHHEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRRNFIKAASCGALLTGALPSVSHAAAENHPPIPGSLGMLYDSTLCVGCQACVTKCQDI
CCCCHHHHHHHCCHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNC
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVPKYDYNNPFGALHKCELCNQK
HHCCCHHHHHCCCCCCCEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
GVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HHHHHHCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEE
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLP
EHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LAVLAGLTVLVRRNTKNDHHDGGDDHES
HHHHHHHHEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]