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The map label for this gene is hyb0

Identifier: 187731765

GI number: 187731765

Start: 3187034

End: 3188152

Strand: Reverse

Name: hyb0

Synonym: SbBS512_E3424

Alternate gene names: 187731765

Gene position: 3188152-3187034 (Counterclockwise)

Preceding gene: 187731332

Following gene: 187732487

Centisome position: 69.07

GC content: 54.24

Gene sequence:

>1119_bases
ATGACTGGAGATAACACCCTCATCCATTCTCACGGCATTAACCGTCGTGATTTCATGAAGCTTTGTGCAGCATTAGCCGC
CACCATGGGGTTAAGTAGCAAAGCCGCTGCAGAGATGGCCGAATCGGTTACTAACCCGCAGCGTCCGCCAGTTATCTGGA
TTGGCGCGCAGGAGTGCACCGGTTGTACGGAATCTCTGCTTCGTGCAACGCATCCAACGGTAGAAAACCTCGTGCTGGAG
ACTATCTCTCTGGAGTATCACGAAGTGCTTTCCGCCGCCTTCGGTCATCAGGTCGAAGAGAACAAACATAACGCACTTGA
GAAGTACAAAGGGCAGTATGTGTTGGTTGTGGATGGTTCCATCCCATTAAAAGATAACGGTATTTATTGCATGGTTGCTG
GTGAGCCGATTGTGGATCACATCCGCAAAGCGGCAGAAGGCGCAGCCGCGATTATCGCTATCGGTTCCTGCTCTGCGTGG
GGCGGTGTTGCCGCAGCTGGAGTTAACCCAACTGGCGCAGTCAGCCTGCAAGAAGTTCTGCCAGGCAAAACCGTTATCAA
TATTCCGGGCTGCCCGCCGAACCCGCACAACTTCCTCGCGACCGTTGCGCACATCATCACTTACGGCAAACCGCCGAAAC
TGGATGACAAAAATCGTCCGACCTTCGCCTATGGCCGTCTGATTCACGAACACTGCGAACGTCGCCCGCACTTCGATGCT
GGTCGTTTTGCCAAAGAGTTCGGTGATGAAGGCCACCGTGAAGGCTGGTGCCTGTACCACCTCGGCTGTAAAGGGCCAGA
AACTTACGGCAACTGCTCAACGCTGCAATTCTGCGATGTTGGCGGCGTGTGGCCGGTAGCGATTGGTCACCCATGCTATG
GCTGTAACGAAGAAGGTATCGGCTTCCATAAAGGCATCCATCAGCTTGCCAACGTCGAAAATCAGACTCCGCGTTCACAG
AAACCGGATGTTAACGCTAAAGAGGGCGGCAACGTCTCTGCAGGCGCTATTGGTTTGCTCGGCGGTGTGGTTGGGCTGGT
TGCCGGTGTCAGCGTGATGGCGGTGCGTGAACTGGGTCGTCAGCAAAAGAAAGATAACGCTGACTCACGGGGAGAATAA

Upstream 100 bases:

>100_bases
TAACCATAATAAATGTGTGGTAAATGGCGCATCGATCGCATTATTGATTTTGCGATTGAAGCAAAATATATGCCAGGTCT
TCGCAACGGAATAACTATAA

Downstream 100 bases:

>100_bases
CCGTGAACAGACGTAATTTTATTAAAGCAGCCTCCTGCGGGGCATTGCTGACGGGCGCGCTGCCGTCTGTCAGTCATGCG
GCTGCTGAAAACCACCCGCC

Product: hydrogenase 2 small subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 small subunit; NiFe hydrogenase

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVTNPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLE
TISLEYHEVLSAAFGHQVEENKHNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKNRPTFAYGRLIHEHCERRPHFDA
GRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQFCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQ
KPDVNAKEGGNVSAGAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE

Sequences:

>Translated_372_residues
MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVTNPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLE
TISLEYHEVLSAAFGHQVEENKHNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKNRPTFAYGRLIHEHCERRPHFDA
GRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQFCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQ
KPDVNAKEGGNVSAGAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE
>Mature_371_residues
TGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVTNPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLET
ISLEYHEVLSAAFGHQVEENKHNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAWG
GVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKNRPTFAYGRLIHEHCERRPHFDAG
RFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQFCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQK
PDVNAKEGGNVSAGAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Periplasmic side. Periplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family

Homologues:

Organism=Escherichia coli, GI1789371, Length=372, Percent_Identity=100, Blast_Score=768, Evalue=0.0,
Organism=Escherichia coli, GI1787206, Length=293, Percent_Identity=41.9795221843003, Blast_Score=261, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MBHT_ECO57 (P69743)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85959
- PIR:   B91114
- RefSeq:   NP_289575.1
- RefSeq:   NP_311909.1
- ProteinModelPortal:   P69743
- SMR:   P69743
- EnsemblBacteria:   EBESCT00000024891
- EnsemblBacteria:   EBESCT00000060407
- GeneID:   916289
- GeneID:   958478
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4351
- KEGG:   ecs:ECs3882
- GeneTree:   EBGT00050000010145
- HOGENOM:   HBG575615
- OMA:   RLIHENC
- ProtClustDB:   PRK10468
- BioCyc:   ECOL83334:ECS3882-MONOMER
- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311
- PIRSF:   PIRSF000310
- PRINTS:   PR00614
- TIGRFAMs:   TIGR00391
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.12.99.6

Molecular weight: Translated: 39652; Mature: 39521

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVTNPQRPPVIWIGAQECT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECHHHC
GCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENKHNALEKYKGQYVLVVDGS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCC
IPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAWGGVAAAGVNPTGAVSLQEVL
CEEECCCEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCCCCCCEEHHHHC
PGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKNRPTFAYGRLIHEHCERRPHFDA
CCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCH
GRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQFCDVGGVWPVAIGHPCYGCNEEGI
HHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCC
GFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSAGAIGLLGGVVGLVAGVSVMAVRELGR
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQKKDNADSRGE
HHHHCCCCCCCC
>Mature Secondary Structure 
TGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVTNPQRPPVIWIGAQECT
CCCCCEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECHHHC
GCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENKHNALEKYKGQYVLVVDGS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCC
IPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAWGGVAAAGVNPTGAVSLQEVL
CEEECCCEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCCCCCCEEHHHHC
PGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKNRPTFAYGRLIHEHCERRPHFDA
CCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCH
GRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQFCDVGGVWPVAIGHPCYGCNEEGI
HHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCC
GFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSAGAIGLLGGVVGLVAGVSVMAVRELGR
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQKKDNADSRGE
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796