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The map label for this gene is hisM [H]

Identifier: 186476916

GI number: 186476916

Start: 2426916

End: 2427629

Strand: Direct

Name: hisM [H]

Synonym: Bphy_2165

Alternate gene names: 186476916

Gene position: 2426916-2427629 (Clockwise)

Preceding gene: 186476915

Following gene: 186476917

Centisome position: 69.76

GC content: 61.06

Gene sequence:

>714_bases
ATGATCGAGCTGATCCAGCAATACTGGCGCAACTATCTGTATACGGACGGTTACCGCTTCACGGGCGTCGCCATCACGCT
GTGGCTGCTGGTGATTTCCATTGGCCTTGGCTTCTGTCTCTCGGTACCGCTCGCCGTGGCGCGCGTGTCGAAGAAGAAGT
GGGTCGCAGGCTCGGTGTGGCTGTACACGTATATCTTCCGCGGCACCCCGTTGTACGTGCAACTGCTGCTCTGCTACACG
GGTTTGTACAGCCTCGAAATTATCCGTTCGAACGCGCTGACCAACGCGTTCTTCCGCGACGGCATGCATTGCACGCTGCT
CGCGTTCACGCTGAACACGTGCGCATACACGACGGAAATCTTTGCCGGCGCGATCAAGGCGACGCCGTATGGCGAGATCG
AAGCGGCGCGCGCGTACGGCATGTCGCAGTTCACGCTGTATCGCCGCGTGATCCTGCCGTCGGCGCTGCGGCGCGCGCTG
CCGTACTACAGCAACGAAGTGATCCTCATGCTGCACGCCACCACGGTCGCCTTCACCGCGACGGTGCCGGACATCCTGAA
GATCGCACGCGACGTCAATTCGGCGACCTACCGTTCGTTCGACGCGTTCGGTATCGCCGCCCTGCTCTATCTGATCATTT
CTTTTGCGCTCGTCTGGCTGTTCCGTCGCGCCGAGCGCCGCTGGCTCGCTTATCTGCGCCCGCAAGGCAAGTAA

Upstream 100 bases:

>100_bases
TGATGGCAGGCGCCATCTATCTGCTCATCACGACGGCTTCGAACTTCGTGCTGATGTACCTCGAAAAACGCTACTCGACT
GGCGTGCGCAAGGCGGAACT

Downstream 100 bases:

>100_bases
CGACGGCGTACAAGCAAGTCAAGGAACCCGATGAACACTCCCGCTACACCGCAAATGCTTTTCGTCGACAACCTGCATAA
GCAGTACGGCGACAACGAAG

Product: polar amino acid ABC transporter inner membrane subunit

Products: ADP; phosphate; L-histidine [Cytoplasm]; ADP; L-ornithine [Cytoplasm]; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MIELIQQYWRNYLYTDGYRFTGVAITLWLLVISIGLGFCLSVPLAVARVSKKKWVAGSVWLYTYIFRGTPLYVQLLLCYT
GLYSLEIIRSNALTNAFFRDGMHCTLLAFTLNTCAYTTEIFAGAIKATPYGEIEAARAYGMSQFTLYRRVILPSALRRAL
PYYSNEVILMLHATTVAFTATVPDILKIARDVNSATYRSFDAFGIAALLYLIISFALVWLFRRAERRWLAYLRPQGK

Sequences:

>Translated_237_residues
MIELIQQYWRNYLYTDGYRFTGVAITLWLLVISIGLGFCLSVPLAVARVSKKKWVAGSVWLYTYIFRGTPLYVQLLLCYT
GLYSLEIIRSNALTNAFFRDGMHCTLLAFTLNTCAYTTEIFAGAIKATPYGEIEAARAYGMSQFTLYRRVILPSALRRAL
PYYSNEVILMLHATTVAFTATVPDILKIARDVNSATYRSFDAFGIAALLYLIISFALVWLFRRAERRWLAYLRPQGK
>Mature_237_residues
MIELIQQYWRNYLYTDGYRFTGVAITLWLLVISIGLGFCLSVPLAVARVSKKKWVAGSVWLYTYIFRGTPLYVQLLLCYT
GLYSLEIIRSNALTNAFFRDGMHCTLLAFTLNTCAYTTEIFAGAIKATPYGEIEAARAYGMSQFTLYRRVILPSALRRAL
PYYSNEVILMLHATTVAFTATVPDILKIARDVNSATYRSFDAFGIAALLYLIISFALVWLFRRAERRWLAYLRPQGK

Specific function: Part of the binding-protein-dependent transport system for histidine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4160

COG function: function code E; ABC-type arginine/histidine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788645, Length=236, Percent_Identity=66.5254237288136, Blast_Score=337, Evalue=5e-94,
Organism=Escherichia coli, GI1787086, Length=178, Percent_Identity=35.3932584269663, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788226, Length=215, Percent_Identity=32.5581395348837, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787030, Length=208, Percent_Identity=30.2884615384615, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787087, Length=221, Percent_Identity=25.3393665158371, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788646, Length=214, Percent_Identity=27.1028037383178, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1786873, Length=224, Percent_Identity=25, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI48994930, Length=143, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786874, Length=214, Percent_Identity=23.3644859813084, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27048; Mature: 27048

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELIQQYWRNYLYTDGYRFTGVAITLWLLVISIGLGFCLSVPLAVARVSKKKWVAGSVW
CHHHHHHHHHHCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LYTYIFRGTPLYVQLLLCYTGLYSLEIIRSNALTNAFFRDGMHCTLLAFTLNTCAYTTEI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FAGAIKATPYGEIEAARAYGMSQFTLYRRVILPSALRRALPYYSNEVILMLHATTVAFTA
HHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHH
TVPDILKIARDVNSATYRSFDAFGIAALLYLIISFALVWLFRRAERRWLAYLRPQGK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIELIQQYWRNYLYTDGYRFTGVAITLWLLVISIGLGFCLSVPLAVARVSKKKWVAGSVW
CHHHHHHHHHHCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LYTYIFRGTPLYVQLLLCYTGLYSLEIIRSNALTNAFFRDGMHCTLLAFTLNTCAYTTEI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
FAGAIKATPYGEIEAARAYGMSQFTLYRRVILPSALRRALPYYSNEVILMLHATTVAFTA
HHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHH
TVPDILKIARDVNSATYRSFDAFGIAALLYLIISFALVWLFRRAERRWLAYLRPQGK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O; ATP; L-ornithine [Periplasm]; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [P

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]