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The map label for this gene is argT [H]

Identifier: 186476914

GI number: 186476914

Start: 2425126

End: 2425902

Strand: Direct

Name: argT [H]

Synonym: Bphy_2163

Alternate gene names: 186476914

Gene position: 2425126-2425902 (Clockwise)

Preceding gene: 186476912

Following gene: 186476915

Centisome position: 69.7

GC content: 61.78

Gene sequence:

>777_bases
ATGAAGAAGTACACACTGTGCGTGGCGCTCGCCGTCATGGCTTCGGCAGCCATGGCGAAAGAATGGAAAACGGTGCGCAT
CGGGGTCGACGCCAGTTATCCGCCGTTCGAATCGATCGCGCCGAACGGCCACATGGTCGGCTTCGACGTCGATCTGACCA
AAGCGCTGTGCGCGAAGATGAACGTGAAGTGCGTGTGGATTGCACAGGACCTCGACGGCATCATCCCGGCGCTCAAGGGC
AAGAAGTACGACATGATCGTGTCGTCGCTGACCGTCACCGACAAGCGCCGCGAGCAGATCGACTTCTCCGACAAACTGTT
CGACGCGCCCGCGCGCATGATCGCAAAGAAGGGCTCCCCGCTGCTGCCGACGGCAGAATCGCTGAAGGGCAAGCACGTCG
GCGTCGAGCAGGGGTCGACGCAGGAAGCGTATGCGAAGGCGTACTGGGAGCCCAGGGGCGTGACGGTCGTGCCTTACCAG
AACCAGGACCAGGTGTACGCGGATCTGGCGACGGGGCGTCTCGACGCTGCGTTGCAGGACGAGCTGCAGGCCGACTCCGG
CTTCCTGAAGACGCCACGCGGCAAGGACTTCGCGTGGGCGGGACGGGAAGTGAAGGACGCAAAGACGATCGGCGACGGTA
CAGCCATCGGCCTGCGCAAGGAAGACGGCGAGCTCAAGGCCATGTTGAACAAGGCGCTCGCCGACATCCACAAAGACGGT
ACGTTCACGAAGCTCGAGAAGCAGTATTTCGACGTCGATATTTTTCGGAGTCGCTGA

Upstream 100 bases:

>100_bases
CAGGCAACGCATTTTTGTCCGCCTAGAATGGCGTCGGCCGGCCGCGAGCGGCCATCGTCAGCGCGCGCAGGCGCCGCGGC
GACGGGAAGTCAGGAGAAAC

Downstream 100 bases:

>100_bases
CGCAATTACCCATTGCCGCAAGCGATGGCAAAAGCTCGGGCAGGCACGGATGGGCCGGCCCGCGAGCTTCGTAATACAGT
CGGACCCAGCAGCAACGCAG

Product: cationic amino acid ABC transporter periplasmic binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKKYTLCVALAVMASAAMAKEWKTVRIGVDASYPPFESIAPNGHMVGFDVDLTKALCAKMNVKCVWIAQDLDGIIPALKG
KKYDMIVSSLTVTDKRREQIDFSDKLFDAPARMIAKKGSPLLPTAESLKGKHVGVEQGSTQEAYAKAYWEPRGVTVVPYQ
NQDQVYADLATGRLDAALQDELQADSGFLKTPRGKDFAWAGREVKDAKTIGDGTAIGLRKEDGELKAMLNKALADIHKDG
TFTKLEKQYFDVDIFRSR

Sequences:

>Translated_258_residues
MKKYTLCVALAVMASAAMAKEWKTVRIGVDASYPPFESIAPNGHMVGFDVDLTKALCAKMNVKCVWIAQDLDGIIPALKG
KKYDMIVSSLTVTDKRREQIDFSDKLFDAPARMIAKKGSPLLPTAESLKGKHVGVEQGSTQEAYAKAYWEPRGVTVVPYQ
NQDQVYADLATGRLDAALQDELQADSGFLKTPRGKDFAWAGREVKDAKTIGDGTAIGLRKEDGELKAMLNKALADIHKDG
TFTKLEKQYFDVDIFRSR
>Mature_258_residues
MKKYTLCVALAVMASAAMAKEWKTVRIGVDASYPPFESIAPNGHMVGFDVDLTKALCAKMNVKCVWIAQDLDGIIPALKG
KKYDMIVSSLTVTDKRREQIDFSDKLFDAPARMIAKKGSPLLPTAESLKGKHVGVEQGSTQEAYAKAYWEPRGVTVVPYQ
NQDQVYADLATGRLDAALQDELQADSGFLKTPRGKDFAWAGREVKDAKTIGDGTAIGLRKEDGELKAMLNKALADIHKDG
TFTKLEKQYFDVDIFRSR

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=258, Percent_Identity=57.3643410852713, Blast_Score=307, Evalue=5e-85,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=51.931330472103, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=36.6533864541833, Blast_Score=160, Evalue=5e-41,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=33.2, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1788228, Length=238, Percent_Identity=30.2521008403361, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787031, Length=245, Percent_Identity=31.4285714285714, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28427; Mature: 28427

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYTLCVALAVMASAAMAKEWKTVRIGVDASYPPFESIAPNGHMVGFDVDLTKALCAKM
CCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHCCCCCCEEEECCHHHHHHHHHC
NVKCVWIAQDLDGIIPALKGKKYDMIVSSLTVTDKRREQIDFSDKLFDAPARMIAKKGSP
CCEEEEEECCCHHHHHHHCCCCCEEEEEHEEECCHHHHHCCCHHHHHHHHHHHHHHCCCC
LLPTAESLKGKHVGVEQGSTQEAYAKAYWEPRGVTVVPYQNQDQVYADLATGRLDAALQD
CCCCHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEHHCCHHHHHHHH
ELQADSGFLKTPRGKDFAWAGREVKDAKTIGDGTAIGLRKEDGELKAMLNKALADIHKDG
HHHCCCCCEECCCCCCCEECCCCCHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
TFTKLEKQYFDVDIFRSR
CHHHHHHHHCCEEEEECC
>Mature Secondary Structure
MKKYTLCVALAVMASAAMAKEWKTVRIGVDASYPPFESIAPNGHMVGFDVDLTKALCAKM
CCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHCCCCCCEEEECCHHHHHHHHHC
NVKCVWIAQDLDGIIPALKGKKYDMIVSSLTVTDKRREQIDFSDKLFDAPARMIAKKGSP
CCEEEEEECCCHHHHHHHCCCCCEEEEEHEEECCHHHHHCCCHHHHHHHHHHHHHHCCCC
LLPTAESLKGKHVGVEQGSTQEAYAKAYWEPRGVTVVPYQNQDQVYADLATGRLDAALQD
CCCCHHHCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEHHCCHHHHHHHH
ELQADSGFLKTPRGKDFAWAGREVKDAKTIGDGTAIGLRKEDGELKAMLNKALADIHKDG
HHHCCCCCEECCCCCCCEECCCCCHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
TFTKLEKQYFDVDIFRSR
CHHHHHHHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]