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The map label for this gene is paaF [H]

Identifier: 186476739

GI number: 186476739

Start: 2235477

End: 2236265

Strand: Direct

Name: paaF [H]

Synonym: Bphy_1984

Alternate gene names: 186476739

Gene position: 2235477-2236265 (Clockwise)

Preceding gene: 186476738

Following gene: 186476740

Centisome position: 64.25

GC content: 66.29

Gene sequence:

>789_bases
ATGGCCGCCGAACTGCTCGCCTCGCGCCCGACCGAAAGCGAATCGACGCTCGTGTTGACACTCTCGAACCCAGGGGCGCG
CAACGCGATGCACCCGGACATGTACGCGGCCGGCATCGAAGCGATGAACACGGCCGAGCGCGACGCGTCGATCGGCGCGA
TCGTCATCACGGGCGCCGACAACTTCTTCTGCGCAGGCGGCAATCTGAACCGCCTGCTCGAGAACCGCGCGAAAGATCCG
TCCGTGCAGGCGCAAAGCATCGATCTGCTCGGCGAATGGATTGCCGCACTGCAGGCGTCGTCGAAGCCGGTAATCGCTGC
CGTCGAAGGCGCGGCGGCGGGTGCAGGTTTTTCGCTGGCGCTCGCATGCGACCTGATCGTCGCCGCCGACGACGCGAAGT
TCGTGATGTCGTACGCGCGTGTCGGCCTCACGCCCGACGGCGGCGGCTCGTGGTTCCTCGCACGTGCGCTGCCGCGTCAG
ATCGCAACGGAAGTGCTGATCGAAGGCAAGCCGATCGGTGCGCCGCGTCTCTATGAACTCGGCGTCGTCAACCGCCTCGC
GAAACCGGGTGCCGTGCGCGATGCGGCTGTCGCGTGGGCCGACGATCTCGGCAAGGTCTCGCCGAATGCAAAGGCACGCA
TCAAAGGCCTGATCGCGGCCGCGGGCGAGCAGTCGCTGCCCGATCAGCTCGTCGCGGAGCGCGACAGCTTTGTCGCGTCG
CTGCATCATCGCGACGGACTCGAAGGCATCACGGCATTCCTCGAAAAGCGCACGCCGAACTACCGATGA

Upstream 100 bases:

>100_bases
CACCGTGGCTCGCACGCCGCGCGCAACTCGGTATGCCGCTCACGCAACGCGATGCCACCGACAATGACGACAACAACTAC
GACACTCAGGAGCACGCATA

Downstream 100 bases:

>100_bases
GCACGCCCGCTTATCAGTTGCCGAAACGCCGCCGCATCTATCTGATGCGGCATGGCGACGTCACCTACTTCGACAAGTCG
GGTGGCACGATCGACCCGGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MAAELLASRPTESESTLVLTLSNPGARNAMHPDMYAAGIEAMNTAERDASIGAIVITGADNFFCAGGNLNRLLENRAKDP
SVQAQSIDLLGEWIAALQASSKPVIAAVEGAAAGAGFSLALACDLIVAADDAKFVMSYARVGLTPDGGGSWFLARALPRQ
IATEVLIEGKPIGAPRLYELGVVNRLAKPGAVRDAAVAWADDLGKVSPNAKARIKGLIAAAGEQSLPDQLVAERDSFVAS
LHHRDGLEGITAFLEKRTPNYR

Sequences:

>Translated_262_residues
MAAELLASRPTESESTLVLTLSNPGARNAMHPDMYAAGIEAMNTAERDASIGAIVITGADNFFCAGGNLNRLLENRAKDP
SVQAQSIDLLGEWIAALQASSKPVIAAVEGAAAGAGFSLALACDLIVAADDAKFVMSYARVGLTPDGGGSWFLARALPRQ
IATEVLIEGKPIGAPRLYELGVVNRLAKPGAVRDAAVAWADDLGKVSPNAKARIKGLIAAAGEQSLPDQLVAERDSFVAS
LHHRDGLEGITAFLEKRTPNYR
>Mature_261_residues
AAELLASRPTESESTLVLTLSNPGARNAMHPDMYAAGIEAMNTAERDASIGAIVITGADNFFCAGGNLNRLLENRAKDPS
VQAQSIDLLGEWIAALQASSKPVIAAVEGAAAGAGFSLALACDLIVAADDAKFVMSYARVGLTPDGGGSWFLARALPRQI
ATEVLIEGKPIGAPRLYELGVVNRLAKPGAVRDAAVAWADDLGKVSPNAKARIKGLIAAAGEQSLPDQLVAERDSFVASL
HHRDGLEGITAFLEKRTPNYR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=30.6201550387597, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=29.0983606557377, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI260274832, Length=207, Percent_Identity=26.5700483091787, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI45643119, Length=207, Percent_Identity=26.5700483091787, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI260275230, Length=207, Percent_Identity=26.5700483091787, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI4502327, Length=263, Percent_Identity=27.7566539923954, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI70995211, Length=259, Percent_Identity=29.3436293436293, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136756, Length=250, Percent_Identity=21.6, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI221136753, Length=250, Percent_Identity=21.6, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI25453481, Length=247, Percent_Identity=21.0526315789474, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI51538573, Length=247, Percent_Identity=21.0526315789474, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4757966, Length=204, Percent_Identity=23.0392156862745, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI51538581, Length=204, Percent_Identity=23.0392156862745, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221307494, Length=250, Percent_Identity=21.6, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI4757968, Length=253, Percent_Identity=22.9249011857708, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI49355787, Length=253, Percent_Identity=22.9249011857708, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI116235487, Length=248, Percent_Identity=23.7903225806452, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI20127408, Length=224, Percent_Identity=25.8928571428571, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI68989263, Length=178, Percent_Identity=28.6516853932584, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=35, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=34.7826086956522, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI221142681, Length=257, Percent_Identity=33.852140077821, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788597, Length=244, Percent_Identity=27.8688524590164, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI87082183, Length=201, Percent_Identity=29.3532338308458, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=188, Percent_Identity=28.7234042553192, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=32.8, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=31.496062992126, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17540306, Length=260, Percent_Identity=26.1538461538462, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=245, Percent_Identity=23.265306122449, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=207, Percent_Identity=29.951690821256, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=168, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=196, Percent_Identity=26.0204081632653, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=224, Percent_Identity=26.3392857142857, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=224, Percent_Identity=26.3392857142857, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=224, Percent_Identity=26.3392857142857, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=243, Percent_Identity=24.6913580246914, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=256, Percent_Identity=24.21875, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=31.7460317460317, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=31.7460317460317, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=30, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=27.0916334661355, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24654903, Length=243, Percent_Identity=23.0452674897119, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28571729, Length=189, Percent_Identity=29.1005291005291, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28571730, Length=189, Percent_Identity=29.1005291005291, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27387; Mature: 27256

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAELLASRPTESESTLVLTLSNPGARNAMHPDMYAAGIEAMNTAERDASIGAIVITGAD
CCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCC
NFFCAGGNLNRLLENRAKDPSVQAQSIDLLGEWIAALQASSKPVIAAVEGAAAGAGFSLA
CEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEE
LACDLIVAADDAKFVMSYARVGLTPDGGGSWFLARALPRQIATEVLIEGKPIGAPRLYEL
EEEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
GVVNRLAKPGAVRDAAVAWADDLGKVSPNAKARIKGLIAAAGEQSLPDQLVAERDSFVAS
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LHHRDGLEGITAFLEKRTPNYR
HHHCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AAELLASRPTESESTLVLTLSNPGARNAMHPDMYAAGIEAMNTAERDASIGAIVITGAD
CCCHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCC
NFFCAGGNLNRLLENRAKDPSVQAQSIDLLGEWIAALQASSKPVIAAVEGAAAGAGFSLA
CEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEE
LACDLIVAADDAKFVMSYARVGLTPDGGGSWFLARALPRQIATEVLIEGKPIGAPRLYEL
EEEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
GVVNRLAKPGAVRDAAVAWADDLGKVSPNAKARIKGLIAAAGEQSLPDQLVAERDSFVAS
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LHHRDGLEGITAFLEKRTPNYR
HHHCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]