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The map label for this gene is potH [H]

Identifier: 186476036

GI number: 186476036

Start: 1432949

End: 1433878

Strand: Reverse

Name: potH [H]

Synonym: Bphy_1277

Alternate gene names: 186476036

Gene position: 1433878-1432949 (Counterclockwise)

Preceding gene: 186476037

Following gene: 186476035

Centisome position: 41.21

GC content: 60.22

Gene sequence:

>930_bases
ATGAAAAGCGCGCTGCATACCTGCATGATGTGGCCTGTGCGCCGCTTCCGTCTGACAGGGCGCACGGCCGTCGTCGCGGG
GCCGTTCGTCTGGCTGCTGCTGTTTTTCCTCGTGCCGTTCCTGCTGGTCGTGAAGATCAGCTTCGCGGACCTGCAGCTCG
GCATTCCGCCGTACACGGAGCTGACGACGTTCAAGGAAGGCGTGATCCATCTCGCGCTCGACCTCTCACACTACGCGTTT
CTGCTGACCGACAGCCTGTACTTCGCGACCTATGTGAACTCGGTGGTCGTCGCGGCGATCTCGACGCTGCTGTGCCTGCT
GCTCGGCTATCCGATGGCGTATTACATCGCGCGCTCGAACCCGGCCACGCGCAACATCCTGATGATGGCCGTGATGCTGC
CGTTCTGGACGTCGTTCCTGATCCGCGTGTATGCGTGGATCGGCATCCTCAAGAACAACGGGCTGCTGAACAACTTCCTG
ATGTCGATCGGGCTGATTCATTCGCCCATCGAGCTGTATCACACGAATACGGCTGTCTATATCGGCATGGTGTATTCGTA
CTTGCCGTTCCTCGTGATGCCGCTCTACGCGCATCTCGTGAAGATGGACCTGACGTTGCTCGAAGCCGCGTACGACCTCG
GCGCGCAACCTTGGACCGCGTTCTGGCGCATTACGCTGCCGTTGTCGAAAAACGGCATCATTGCGGGCTGTTTGCTCGTG
TTCATCCCCGCGGTGGGCGAGTACGTGATTCCTGAACTGCTCGGCGGCGCGAATACGCTGATGATCGGCCGCGTGATGTG
GAATGAGTTCTTCGACAACGCCGACTGGCCGATGGCATCCGCCGTCACCTGCGCAATGGTGCTGCTGCTGCTCGTGCCGA
TGGCGCTGTTCCAGTACTCGCAGGCGAAGGAACTGGAGGAGAAGCGCTGA

Upstream 100 bases:

>100_bases
CGAACGTGTCGAGCCTCGCGCTGTCGGAAATCGAAACGCCTACCTGGGGCGATGAAGTGTATGTGCGCTGGAGCGCGTCG
GCGGGCGTGGTGCTGACGTC

Downstream 100 bases:

>100_bases
TGAAGCCCAATCGTGTATTGCAGTTCATCGCGCTCGCGATCGGCTTTGCGTTCCTCTACGTGCCGATCATCAGCCTCGTG
GTGTATTCGTTCAACGAGTC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKSALHTCMMWPVRRFRLTGRTAVVAGPFVWLLLFFLVPFLLVVKISFADLQLGIPPYTELTTFKEGVIHLALDLSHYAF
LLTDSLYFATYVNSVVVAAISTLLCLLLGYPMAYYIARSNPATRNILMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFL
MSIGLIHSPIELYHTNTAVYIGMVYSYLPFLVMPLYAHLVKMDLTLLEAAYDLGAQPWTAFWRITLPLSKNGIIAGCLLV
FIPAVGEYVIPELLGGANTLMIGRVMWNEFFDNADWPMASAVTCAMVLLLLVPMALFQYSQAKELEEKR

Sequences:

>Translated_309_residues
MKSALHTCMMWPVRRFRLTGRTAVVAGPFVWLLLFFLVPFLLVVKISFADLQLGIPPYTELTTFKEGVIHLALDLSHYAF
LLTDSLYFATYVNSVVVAAISTLLCLLLGYPMAYYIARSNPATRNILMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFL
MSIGLIHSPIELYHTNTAVYIGMVYSYLPFLVMPLYAHLVKMDLTLLEAAYDLGAQPWTAFWRITLPLSKNGIIAGCLLV
FIPAVGEYVIPELLGGANTLMIGRVMWNEFFDNADWPMASAVTCAMVLLLLVPMALFQYSQAKELEEKR
>Mature_309_residues
MKSALHTCMMWPVRRFRLTGRTAVVAGPFVWLLLFFLVPFLLVVKISFADLQLGIPPYTELTTFKEGVIHLALDLSHYAF
LLTDSLYFATYVNSVVVAAISTLLCLLLGYPMAYYIARSNPATRNILMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFL
MSIGLIHSPIELYHTNTAVYIGMVYSYLPFLVMPLYAHLVKMDLTLLEAAYDLGAQPWTAFWRITLPLSKNGIIAGCLLV
FIPAVGEYVIPELLGGANTLMIGRVMWNEFFDNADWPMASAVTCAMVLLLLVPMALFQYSQAKELEEKR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=288, Percent_Identity=57.2916666666667, Blast_Score=324, Evalue=5e-90,
Organism=Escherichia coli, GI1787369, Length=279, Percent_Identity=40.1433691756272, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1787713, Length=213, Percent_Identity=29.5774647887324, Blast_Score=104, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34845; Mature: 34845

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSALHTCMMWPVRRFRLTGRTAVVAGPFVWLLLFFLVPFLLVVKISFADLQLGIPPYTE
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHH
LTTFKEGVIHLALDLSHYAFLLTDSLYFATYVNSVVVAAISTLLCLLLGYPMAYYIARSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCC
PATRNILMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLMSIGLIHSPIELYHTNTAVY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCHHHH
IGMVYSYLPFLVMPLYAHLVKMDLTLLEAAYDLGAQPWTAFWRITLPLSKNGIIAGCLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHH
FIPAVGEYVIPELLGGANTLMIGRVMWNEFFDNADWPMASAVTCAMVLLLLVPMALFQYS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QAKELEEKR
HHHHHHHCC
>Mature Secondary Structure
MKSALHTCMMWPVRRFRLTGRTAVVAGPFVWLLLFFLVPFLLVVKISFADLQLGIPPYTE
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHH
LTTFKEGVIHLALDLSHYAFLLTDSLYFATYVNSVVVAAISTLLCLLLGYPMAYYIARSN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCC
PATRNILMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLMSIGLIHSPIELYHTNTAVY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCHHHH
IGMVYSYLPFLVMPLYAHLVKMDLTLLEAAYDLGAQPWTAFWRITLPLSKNGIIAGCLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHH
FIPAVGEYVIPELLGGANTLMIGRVMWNEFFDNADWPMASAVTCAMVLLLLVPMALFQYS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QAKELEEKR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]