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The map label for this gene is potI [H]

Identifier: 186476035

GI number: 186476035

Start: 1432131

End: 1432949

Strand: Reverse

Name: potI [H]

Synonym: Bphy_1276

Alternate gene names: 186476035

Gene position: 1432949-1432131 (Counterclockwise)

Preceding gene: 186476036

Following gene: 186476033

Centisome position: 41.19

GC content: 61.05

Gene sequence:

>819_bases
ATGAAGCCCAATCGTGTATTGCAGTTCATCGCGCTCGCGATCGGCTTTGCGTTCCTCTACGTGCCGATCATCAGCCTCGT
GGTGTATTCGTTCAACGAGTCGCAGCTCGTGACGGTGTGGACGCGCTTTTCGACCCGCTGGTACGCTGCGTTGCTGCAGG
ACGAGGAACTGATCAATGCCGCCTGGCTGTCGTTGCGGGTGGCGTTGCTGACGGCGTTCGCGTCGGTGATCATCGGCACG
TGGGCGGGCTTCGTGCTCGCGCGCATGGGGCGGTTTCGCGGCTTCACGCTGTACACCGGGATGATCAACGCGCCGCTCGT
GATTCCCGAAGTGATCCAGGGCATTTCGCTGCTGCTGCTCTTCATCGAAATGGGCAAGTGGCTTGGCTGGCCTGCGGGGC
GCGGGATCTTCACGATCTGGATCGGCCACGTGATGCTGTGTATTTCCTACGTGGCGATTATCGTGCAGTCGCGCGTGCGT
GACCTGCATCCGTCGCTTGAGGAAGCTGCACTGGATCTTGGTGCCACGCCGTTGCGTGTATTTTTCTTCATCACGCTGCC
GCTGATCTCGCAGGCCCTGGTGGCCGGGTGGCTGCTGTCTTTTACGCTGTCGATCGACGATCTGGTGTTGTCGGCATTCC
TGTCGGGCCCGGGCTCGACGACGCTGCCGCTGGTTGTATTTTCGCGCGTGCGTCTGGGGCTGAATCCGGAAATGAATGCG
CTCGCGACGCTCTTCATTGCCTTCGTGACGGTGGGCGTCGTCGTCGCGAACCACTTTATGCAGCGCGCGGAGAAGCGGCG
GCTGGCAATGGCAATCTAG

Upstream 100 bases:

>100_bases
CGATGGCATCCGCCGTCACCTGCGCAATGGTGCTGCTGCTGCTCGTGCCGATGGCGCTGTTCCAGTACTCGCAGGCGAAG
GAACTGGAGGAGAAGCGCTG

Downstream 100 bases:

>100_bases
TTCAAGCTGCGACGAGAAATTCAATGCAGATGGCTGGCGCCCGCCAGGACGCCGGCTTTTTTCATCAAGATAATCGGGCG
TGCAGGCTTAGATCCGATAA

Product: ornithine carbamoyltransferase

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKPNRVLQFIALAIGFAFLYVPIISLVVYSFNESQLVTVWTRFSTRWYAALLQDEELINAAWLSLRVALLTAFASVIIGT
WAGFVLARMGRFRGFTLYTGMINAPLVIPEVIQGISLLLLFIEMGKWLGWPAGRGIFTIWIGHVMLCISYVAIIVQSRVR
DLHPSLEEAALDLGATPLRVFFFITLPLISQALVAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIAFVTVGVVVANHFMQRAEKRRLAMAI

Sequences:

>Translated_272_residues
MKPNRVLQFIALAIGFAFLYVPIISLVVYSFNESQLVTVWTRFSTRWYAALLQDEELINAAWLSLRVALLTAFASVIIGT
WAGFVLARMGRFRGFTLYTGMINAPLVIPEVIQGISLLLLFIEMGKWLGWPAGRGIFTIWIGHVMLCISYVAIIVQSRVR
DLHPSLEEAALDLGATPLRVFFFITLPLISQALVAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIAFVTVGVVVANHFMQRAEKRRLAMAI
>Mature_272_residues
MKPNRVLQFIALAIGFAFLYVPIISLVVYSFNESQLVTVWTRFSTRWYAALLQDEELINAAWLSLRVALLTAFASVIIGT
WAGFVLARMGRFRGFTLYTGMINAPLVIPEVIQGISLLLLFIEMGKWLGWPAGRGIFTIWIGHVMLCISYVAIIVQSRVR
DLHPSLEEAALDLGATPLRVFFFITLPLISQALVAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIAFVTVGVVVANHFMQRAEKRRLAMAI

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=261, Percent_Identity=55.9386973180077, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1787368, Length=239, Percent_Identity=37.2384937238494, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787714, Length=228, Percent_Identity=32.4561403508772, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788764, Length=213, Percent_Identity=30.5164319248826, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30163; Mature: 30163

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNRVLQFIALAIGFAFLYVPIISLVVYSFNESQLVTVWTRFSTRWYAALLQDEELINA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
AWLSLRVALLTAFASVIIGTWAGFVLARMGRFRGFTLYTGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHHHHHHH
FIEMGKWLGWPAGRGIFTIWIGHVMLCISYVAIIVQSRVRDLHPSLEEAALDLGATPLRV
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHH
FFFITLPLISQALVAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIAFVTVGVVVANHFMQRAEKRRLAMAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPNRVLQFIALAIGFAFLYVPIISLVVYSFNESQLVTVWTRFSTRWYAALLQDEELINA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
AWLSLRVALLTAFASVIIGTWAGFVLARMGRFRGFTLYTGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHHHHHHH
FIEMGKWLGWPAGRGIFTIWIGHVMLCISYVAIIVQSRVRDLHPSLEEAALDLGATPLRV
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHH
FFFITLPLISQALVAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIAFVTVGVVVANHFMQRAEKRRLAMAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]