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The map label for this gene is purR [H]

Identifier: 186475993

GI number: 186475993

Start: 1385485

End: 1386516

Strand: Reverse

Name: purR [H]

Synonym: Bphy_1234

Alternate gene names: 186475993

Gene position: 1386516-1385485 (Counterclockwise)

Preceding gene: 186475994

Following gene: 186475992

Centisome position: 39.85

GC content: 66.57

Gene sequence:

>1032_bases
ATGGCGACGATCAAGGATGTGGCGGCGATAGCCGGCGTGTCGTTCACGACGGTGTCGCATGTCGTGAACAATACGCGTCC
GGTGTCGGCGGACGTGCGCTCGAAGGTGGAACTGGCAATCCGCCAGCTCAACTACGTGCCGTCCGCCGTCGCGCGGTCGC
TGAAGGCCCGTTCGACGGCGACGATCGGGCTCGTCGTGCCCAACAGCACGAACCCGTACTTCGCGGAACTCGCGCGCGGC
ATCGAAGACGGTTGTGCGCGCAATGGCTACTGCGTGTTTTTCTGCAATTCGGACGACGATCCCGCGAAGCAGCGCAACTA
TCTGCGCGTGCTGCAGGAAAAGCGCATCGACGGGCTGATCGTCGCGTCCGCGGGCGACGACGCGACGCTGGCGCAGACGC
TCGCTGATTCGCGCGAGCCGCTCGTCATCGTCGACCGGAACATCGAAGGCCTGTCCGCCGATCTCGTCCAGATCGATCAT
GAAAAAGGCGCCTATCTGGCGACGCGCCATCTGCTGCAGCTCGGCCATTCGAAGATCGGCTGCATCACGGGCCCGGTCGA
AACGGCTGTCAGCGCGATGCGGGTGCACGGCTTCATCCGGGCGATGGCCGAACGCGGCATCGAGATCGCGCCGAACGGCA
TCGTCGAAAGCGACTTCTCCGGTAGCGGCGGCTACCGTGCTGCCGCCCAGCTGTTCGACACGGTGCAGCCGACGGCGATC
TTCGCGGGCAACGACATGATGGGCATCGGCGCGTTGCGCGCCGCGGCGGAGCGCGGCCTGCGCGTGCCGCAGGACTGCTC
GGTGATCGGCTTCGACGACATCGAACTGGGGCGTTTCACCTATCCGGCGCTGTCCACTGTCGGCCAGTCGGTGCGCGCAC
TCGGCGAAATGGCCGCGCAGACGCTGATCGAGCGAATCAGCGGTGAGTCGACGGGCGCGTTGTTCCGCCGCCGCGTGATC
GCGCCGCGATTGATCCTGCGCGAATCGACGGCCGCATTCGCGGGCGCGCGCCGCACGGCTCAGGCGGCATAA

Upstream 100 bases:

>100_bases
CGGGACGGCGGCCGCCTGGCAAGAACCTAACGAGAGCACCGCGGATGCAGCATGAGCTGCATTGGCCAGCAGTAAAAAAC
ACGCAGGGGAGTATCAGCTT

Downstream 100 bases:

>100_bases
GACACGCTCTCATCGGGCGCAAGCGGGTATCGCATGCCTGTCTGTGCCCAACAGAAGACAGCAAGGAGGCTGCAGTGGCA
ACGAATGAAGCGCGCGGACA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MATIKDVAAIAGVSFTTVSHVVNNTRPVSADVRSKVELAIRQLNYVPSAVARSLKARSTATIGLVVPNSTNPYFAELARG
IEDGCARNGYCVFFCNSDDDPAKQRNYLRVLQEKRIDGLIVASAGDDATLAQTLADSREPLVIVDRNIEGLSADLVQIDH
EKGAYLATRHLLQLGHSKIGCITGPVETAVSAMRVHGFIRAMAERGIEIAPNGIVESDFSGSGGYRAAAQLFDTVQPTAI
FAGNDMMGIGALRAAAERGLRVPQDCSVIGFDDIELGRFTYPALSTVGQSVRALGEMAAQTLIERISGESTGALFRRRVI
APRLILRESTAAFAGARRTAQAA

Sequences:

>Translated_343_residues
MATIKDVAAIAGVSFTTVSHVVNNTRPVSADVRSKVELAIRQLNYVPSAVARSLKARSTATIGLVVPNSTNPYFAELARG
IEDGCARNGYCVFFCNSDDDPAKQRNYLRVLQEKRIDGLIVASAGDDATLAQTLADSREPLVIVDRNIEGLSADLVQIDH
EKGAYLATRHLLQLGHSKIGCITGPVETAVSAMRVHGFIRAMAERGIEIAPNGIVESDFSGSGGYRAAAQLFDTVQPTAI
FAGNDMMGIGALRAAAERGLRVPQDCSVIGFDDIELGRFTYPALSTVGQSVRALGEMAAQTLIERISGESTGALFRRRVI
APRLILRESTAAFAGARRTAQAA
>Mature_342_residues
ATIKDVAAIAGVSFTTVSHVVNNTRPVSADVRSKVELAIRQLNYVPSAVARSLKARSTATIGLVVPNSTNPYFAELARGI
EDGCARNGYCVFFCNSDDDPAKQRNYLRVLQEKRIDGLIVASAGDDATLAQTLADSREPLVIVDRNIEGLSADLVQIDHE
KGAYLATRHLLQLGHSKIGCITGPVETAVSAMRVHGFIRAMAERGIEIAPNGIVESDFSGSGGYRAAAQLFDTVQPTAIF
AGNDMMGIGALRAAAERGLRVPQDCSVIGFDDIELGRFTYPALSTVGQSVRALGEMAAQTLIERISGESTGALFRRRVIA
PRLILRESTAAFAGARRTAQAA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=42.814371257485, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1790194, Length=338, Percent_Identity=41.1242603550296, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1790369, Length=335, Percent_Identity=34.9253731343284, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=35.1351351351351, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789202, Length=289, Percent_Identity=35.2941176470588, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1786540, Length=334, Percent_Identity=31.437125748503, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=30.5389221556886, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=29.3375394321767, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI48994940, Length=309, Percent_Identity=27.5080906148867, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787906, Length=343, Percent_Identity=26.8221574344023, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789456, Length=317, Percent_Identity=28.391167192429, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786268, Length=310, Percent_Identity=27.4193548387097, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790715, Length=310, Percent_Identity=25.1612903225806, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790689, Length=312, Percent_Identity=25.6410256410256, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790192, Length=292, Percent_Identity=26.027397260274, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36547; Mature: 36416

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAAIAGVSFTTVSHVVNNTRPVSADVRSKVELAIRQLNYVPSAVARSLKARSTA
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCE
TIGLVVPNSTNPYFAELARGIEDGCARNGYCVFFCNSDDDPAKQRNYLRVLQEKRIDGLI
EEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEE
VASAGDDATLAQTLADSREPLVIVDRNIEGLSADLVQIDHEKGAYLATRHLLQLGHSKIG
EEECCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHEEEECCCCHHHHHHHHHHHCCCCCC
CITGPVETAVSAMRVHGFIRAMAERGIEIAPNGIVESDFSGSGGYRAAAQLFDTVQPTAI
EECCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
FAGNDMMGIGALRAAAERGLRVPQDCSVIGFDDIELGRFTYPALSTVGQSVRALGEMAAQ
EECCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TLIERISGESTGALFRRRVIAPRLILRESTAAFAGARRTAQAA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
ATIKDVAAIAGVSFTTVSHVVNNTRPVSADVRSKVELAIRQLNYVPSAVARSLKARSTA
CCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCE
TIGLVVPNSTNPYFAELARGIEDGCARNGYCVFFCNSDDDPAKQRNYLRVLQEKRIDGLI
EEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEE
VASAGDDATLAQTLADSREPLVIVDRNIEGLSADLVQIDHEKGAYLATRHLLQLGHSKIG
EEECCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHEEEECCCCHHHHHHHHHHHCCCCCC
CITGPVETAVSAMRVHGFIRAMAERGIEIAPNGIVESDFSGSGGYRAAAQLFDTVQPTAI
EECCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
FAGNDMMGIGALRAAAERGLRVPQDCSVIGFDDIELGRFTYPALSTVGQSVRALGEMAAQ
EECCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TLIERISGESTGALFRRRVIAPRLILRESTAAFAGARRTAQAA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA