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The map label for this gene is gcvH

Identifier: 183222534

GI number: 183222534

Start: 3299827

End: 3300219

Strand: Direct

Name: gcvH

Synonym: LEPBI_I3189

Alternate gene names: 183222534

Gene position: 3299827-3300219 (Clockwise)

Preceding gene: 183222533

Following gene: 183222535

Centisome position: 91.67

GC content: 41.22

Gene sequence:

>393_bases
ATGGCAGACACACAAGCAAAAGACGGTTATTATTATACTGAAAAACACGAATGGGTGAAGGTAGAGGGCGATGTGGCTCT
TATTGGAATCACGGATTTCGCACAAAACGCATTAGGTGACATTGTTTTCATCGACCTTCCAAAACCTGGAAAACAAATCA
AAGCAAAAGATAGCCTTGGAACCATTGAATCTGTGAAAGCAGCCGAGGATTTGTATTCCCCTATCTCTGGCGAAGTGGTC
GAAACCAATGCAACACTTGGTTCCAATCCCCAAGCAGTGAACGCAGAACCATTTGATACTTGGATGGTGAAATTAAAAAA
CATCCAAACCTCTGAGCTTGGTGGTTTATTAACTGCTGCACAATACAAAGAATACGTTTCGAAATTGGATTAA

Upstream 100 bases:

>100_bases
ATTTGTGGAGATTCGTGGGCAGAAGAAATTGGCTAAAGTAGAGACTGGTGCCTTTGTCCAAGGCAGTGTCCGAAACAATC
GCTAATGTAAGGAAAAAACC

Downstream 100 bases:

>100_bases
TAGGAGTTATTTGCAAGTGAGTTCCGTACAACCTACATCACCGATCCATTCCCCTTACGAAGAAACACTTGAACCAAGTG
ATACCTTCCTTCGCCGTCAT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MADTQAKDGYYYTEKHEWVKVEGDVALIGITDFAQNALGDIVFIDLPKPGKQIKAKDSLGTIESVKAAEDLYSPISGEVV
ETNATLGSNPQAVNAEPFDTWMVKLKNIQTSELGGLLTAAQYKEYVSKLD

Sequences:

>Translated_130_residues
MADTQAKDGYYYTEKHEWVKVEGDVALIGITDFAQNALGDIVFIDLPKPGKQIKAKDSLGTIESVKAAEDLYSPISGEVV
ETNATLGSNPQAVNAEPFDTWMVKLKNIQTSELGGLLTAAQYKEYVSKLD
>Mature_129_residues
ADTQAKDGYYYTEKHEWVKVEGDVALIGITDFAQNALGDIVFIDLPKPGKQIKAKDSLGTIESVKAAEDLYSPISGEVVE
TNATLGSNPQAVNAEPFDTWMVKLKNIQTSELGGLLTAAQYKEYVSKLD

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=44.1666666666667, Blast_Score=115, Evalue=8e-27,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=44.1666666666667, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789271, Length=114, Percent_Identity=45.6140350877193, Blast_Score=105, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=124, Percent_Identity=44.3548387096774, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17507493, Length=121, Percent_Identity=48.7603305785124, Blast_Score=115, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6319272, Length=126, Percent_Identity=39.6825396825397, Blast_Score=100, Evalue=8e-23,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=46.6101694915254, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_LEPBA (B0SGN9)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001964124.1
- ProteinModelPortal:   B0SGN9
- SMR:   B0SGN9
- GeneID:   6386880
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_3077
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   LBIF355278:LBF_3077-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14171; Mature: 14040

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTQAKDGYYYTEKHEWVKVEGDVALIGITDFAQNALGDIVFIDLPKPGKQIKAKDSLG
CCCCCCCCCEEECCCCCEEEEECCEEEEEEHHHHHHHCCCEEEEECCCCCCCEECCCCCC
TIESVKAAEDLYSPISGEVVETNATLGSNPQAVNAEPFDTWMVKLKNIQTSELGGLLTAA
CHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCHHHHH
QYKEYVSKLD
HHHHHHHHCC
>Mature Secondary Structure 
ADTQAKDGYYYTEKHEWVKVEGDVALIGITDFAQNALGDIVFIDLPKPGKQIKAKDSLG
CCCCCCCCEEECCCCCEEEEECCEEEEEEHHHHHHHCCCEEEEECCCCCCCEECCCCCC
TIESVKAAEDLYSPISGEVVETNATLGSNPQAVNAEPFDTWMVKLKNIQTSELGGLLTAA
CHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCHHHHH
QYKEYVSKLD
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA