Click here to switch to the map view.

The map label for this gene is gcvT

Identifier: 183222533

GI number: 183222533

Start: 3298699

End: 3299811

Strand: Direct

Name: gcvT

Synonym: LEPBI_I3188

Alternate gene names: 183222533

Gene position: 3298699-3299811 (Clockwise)

Preceding gene: 183222531

Following gene: 183222534

Centisome position: 91.64

GC content: 41.6

Gene sequence:

>1113_bases
GTGGAACTAAAACAAACACCCCTACACTCAATTCATAAAGAAATGGGAGCCAAGATGGTTCCTTTCGGCGGATGGGACAT
GCCCGTTCAGTATACAGGTATCATCCAAGAACATTTGGCAACAAGAGCAAACGCAGGCATCTTCGATGTTTCCCATATGG
GTGAAATTTTTGTCACTGGGGATGCCAATGATGTTTTGGATTTTTTAGAATCTGTAACATGTAATACGATCTCTACCATG
AAAGAAGGCCAAGTGCAATACAATGCAGTGGTGAATGAAGTGGGTGGGCTCGTGGATGATATCACTGTATATAAATTCAA
TGATACAAAATATATGATTTGTTCGAATGCATCCAACTTCGAGGCAGTGACCCAACATTTACTTAAGTATGTGAAAGGAA
ACGTTTCGATTGCTAATGATAGTAAAAATTGGCACCAAATCGCCTTACAAGGTCCAAAAGCTGATGCAATCTTTACAAAA
TACTTAGGAAAAGATTTATCTTCCATTTTATATTATCATTTTGAAGAAATGAATTGGAGAGGTGAGACCATCATTGTATC
TCGCACAGGTTATACGGGCGAAGATGGATTTGAAATTTATACTTCCAATGCACTTGGTGTGACTCTTTGGAAAGAGTTAC
TCGAAATCGGAAAGGATTTTGGTCTAGTTCCAGTTGGACTTGGGGCAAGGGACACACTCCGCCTCGAAGCCAAGTATCCT
TTGTATGGACATGAATTGAATGCAGAGTGGACACCCGTAGAATCCGGAATCAATTTCATCGTCAAAGAAAAATCCAAACC
CTATCTCGGTTATGACCGCATCATCGCTGATAAAAAAAATGGTCCCAAATCAAAAGTCGTTGGCGTCCGCCTCTTGGAAC
CAGGAGTTCTACGAGAAAATTTCCCTATTTTTGCAGCAGATGGGAAAGAAATTGGCAAAACAACCTCGGGAACCCATTCC
CCTAGCCGGAAAGAATCCTTGGGACTTGCCATTCTCCAAACCGAATTCGCAAAAAACCAAACGGAAGTATTTGTGGAGAT
TCGTGGGCAGAAGAAATTGGCTAAAGTAGAGACTGGTGCCTTTGTCCAAGGCAGTGTCCGAAACAATCGCTAA

Upstream 100 bases:

>100_bases
ATGCGGTAGACTTCTCTTTGCATAATGGGTTAAAAAAAATTCAATTCCATAACGATGAAACCATTTTTCTATGGTAGAAG
AGTCTACATAAGAGAACACC

Downstream 100 bases:

>100_bases
TGTAAGGAAAAAACCATGGCAGACACACAAGCAAAAGACGGTTATTATTATACTGAAAAACACGAATGGGTGAAGGTAGA
GGGCGATGTGGCTCTTATTG

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MELKQTPLHSIHKEMGAKMVPFGGWDMPVQYTGIIQEHLATRANAGIFDVSHMGEIFVTGDANDVLDFLESVTCNTISTM
KEGQVQYNAVVNEVGGLVDDITVYKFNDTKYMICSNASNFEAVTQHLLKYVKGNVSIANDSKNWHQIALQGPKADAIFTK
YLGKDLSSILYYHFEEMNWRGETIIVSRTGYTGEDGFEIYTSNALGVTLWKELLEIGKDFGLVPVGLGARDTLRLEAKYP
LYGHELNAEWTPVESGINFIVKEKSKPYLGYDRIIADKKNGPKSKVVGVRLLEPGVLRENFPIFAADGKEIGKTTSGTHS
PSRKESLGLAILQTEFAKNQTEVFVEIRGQKKLAKVETGAFVQGSVRNNR

Sequences:

>Translated_370_residues
MELKQTPLHSIHKEMGAKMVPFGGWDMPVQYTGIIQEHLATRANAGIFDVSHMGEIFVTGDANDVLDFLESVTCNTISTM
KEGQVQYNAVVNEVGGLVDDITVYKFNDTKYMICSNASNFEAVTQHLLKYVKGNVSIANDSKNWHQIALQGPKADAIFTK
YLGKDLSSILYYHFEEMNWRGETIIVSRTGYTGEDGFEIYTSNALGVTLWKELLEIGKDFGLVPVGLGARDTLRLEAKYP
LYGHELNAEWTPVESGINFIVKEKSKPYLGYDRIIADKKNGPKSKVVGVRLLEPGVLRENFPIFAADGKEIGKTTSGTHS
PSRKESLGLAILQTEFAKNQTEVFVEIRGQKKLAKVETGAFVQGSVRNNR
>Mature_370_residues
MELKQTPLHSIHKEMGAKMVPFGGWDMPVQYTGIIQEHLATRANAGIFDVSHMGEIFVTGDANDVLDFLESVTCNTISTM
KEGQVQYNAVVNEVGGLVDDITVYKFNDTKYMICSNASNFEAVTQHLLKYVKGNVSIANDSKNWHQIALQGPKADAIFTK
YLGKDLSSILYYHFEEMNWRGETIIVSRTGYTGEDGFEIYTSNALGVTLWKELLEIGKDFGLVPVGLGARDTLRLEAKYP
LYGHELNAEWTPVESGINFIVKEKSKPYLGYDRIIADKKNGPKSKVVGVRLLEPGVLRENFPIFAADGKEIGKTTSGTHS
PSRKESLGLAILQTEFAKNQTEVFVEIRGQKKLAKVETGAFVQGSVRNNR

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=369, Percent_Identity=31.4363143631436, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI257796258, Length=354, Percent_Identity=31.3559322033898, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI257796254, Length=365, Percent_Identity=29.3150684931507, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI257796256, Length=308, Percent_Identity=28.8961038961039, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI197927446, Length=334, Percent_Identity=25.1497005988024, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI21361378, Length=334, Percent_Identity=25.1497005988024, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI24797151, Length=358, Percent_Identity=25.1396648044693, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI194306651, Length=298, Percent_Identity=22.1476510067114, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1789272, Length=370, Percent_Identity=31.6216216216216, Blast_Score=175, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17560118, Length=376, Percent_Identity=31.6489361702128, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32563613, Length=305, Percent_Identity=25.9016393442623, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994052, Length=337, Percent_Identity=22.5519287833828, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994045, Length=337, Percent_Identity=22.5519287833828, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320222, Length=371, Percent_Identity=35.0404312668464, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI20129441, Length=385, Percent_Identity=32.4675324675325, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI28571104, Length=272, Percent_Identity=22.4264705882353, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20130091, Length=359, Percent_Identity=22.5626740947075, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_LEPBA (B0SGN8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001964123.1
- ProteinModelPortal:   B0SGN8
- SMR:   B0SGN8
- GeneID:   6386885
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_3076
- HOGENOM:   HBG299834
- OMA:   KALYGGM
- ProtClustDB:   PRK00389
- BioCyc:   LBIF355278:LBF_3076-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 41111; Mature: 41111

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKQTPLHSIHKEMGAKMVPFGGWDMPVQYTGIIQEHLATRANAGIFDVSHMGEIFVTG
CCCCCCHHHHHHHHHCCEECCCCCCCCCEEHHHHHHHHHHHHCCCCEEECCCCCCEEEEC
DANDVLDFLESVTCNTISTMKEGQVQYNAVVNEVGGLVDDITVYKFNDTKYMICSNASNF
CHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCCHHHCEEEEEECCCEEEEECCCCCH
EAVTQHLLKYVKGNVSIANDSKNWHQIALQGPKADAIFTKYLGKDLSSILYYHFEEMNWR
HHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GETIIVSRTGYTGEDGFEIYTSNALGVTLWKELLEIGKDFGLVPVGLGARDTLRLEAKYP
CCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECC
LYGHELNAEWTPVESGINFIVKEKSKPYLGYDRIIADKKNGPKSKVVGVRLLEPGVLREN
CCCCCCCCCCCCHHCCCEEEEECCCCCCCCHHHEEECCCCCCCCCEEEEEEECCCCCCCC
FPIFAADGKEIGKTTSGTHSPSRKESLGLAILQTEFAKNQTEVFVEIRGQKKLAKVETGA
CCEEEECCHHHCCCCCCCCCCCCHHHCCEEEEEHHHCCCCEEEEEEECCCCCEEEECCCC
FVQGSVRNNR
EEECCCCCCC
>Mature Secondary Structure
MELKQTPLHSIHKEMGAKMVPFGGWDMPVQYTGIIQEHLATRANAGIFDVSHMGEIFVTG
CCCCCCHHHHHHHHHCCEECCCCCCCCCEEHHHHHHHHHHHHCCCCEEECCCCCCEEEEC
DANDVLDFLESVTCNTISTMKEGQVQYNAVVNEVGGLVDDITVYKFNDTKYMICSNASNF
CHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCCHHHCEEEEEECCCEEEEECCCCCH
EAVTQHLLKYVKGNVSIANDSKNWHQIALQGPKADAIFTKYLGKDLSSILYYHFEEMNWR
HHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GETIIVSRTGYTGEDGFEIYTSNALGVTLWKELLEIGKDFGLVPVGLGARDTLRLEAKYP
CCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECC
LYGHELNAEWTPVESGINFIVKEKSKPYLGYDRIIADKKNGPKSKVVGVRLLEPGVLREN
CCCCCCCCCCCCHHCCCEEEEECCCCCCCCHHHEEECCCCCCCCCEEEEEEECCCCCCCC
FPIFAADGKEIGKTTSGTHSPSRKESLGLAILQTEFAKNQTEVFVEIRGQKKLAKVETGA
CCEEEECCHHHCCCCCCCCCCCCHHHCCEEEEEHHHCCCCEEEEEEECCCCCEEEECCCC
FVQGSVRNNR
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA