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The map label for this gene is metK [H]

Identifier: 183222316

GI number: 183222316

Start: 3072150

End: 3073316

Strand: Reverse

Name: metK [H]

Synonym: LEPBI_I2968

Alternate gene names: 183222316

Gene position: 3073316-3072150 (Counterclockwise)

Preceding gene: 183222317

Following gene: 183222315

Centisome position: 85.38

GC content: 43.44

Gene sequence:

>1167_bases
ATGTCCTCTCTAAAAAACTATATCTTCACTTCTGAGTCCGTATCTGAAGGACACCCAGACAAAGTCTGTGACCAAATTTC
TGATGCCATTCTCGATGCGTATTTAGCGCAAGATCCAAAATCCCGTGTTGCTTGTGAAACCCTCGTGACCACAAACTTAG
TCGTCATTGCTGGTGAGATTACAAGCAAAGGAAAAGTTGACACACAAGAAATCGCCCGTGATGTGATTCGTAAAATTGGA
TACAACGACATCAATATGTACTTTGATGCAGACTTTGCTGTGGTTGCCTCTCACGTGCATGCGCAGTCACCTGATATCGC
CCAAGGGGTGAATGAAGGGGAAGGCCTCCACACAGAACAAGGTGCTGGCGACCAAGGTTTGATGTTTGGTTTTGCGATTG
CAGAAACCCCTGAACTCATGCCGGCTCCACTCTATTACTCACATAAACTTTTAGAGCATTTAGCAGAACTTCGTCACACA
AACAAAATTGATTGGCTTCGACCTGATGCAAAATCACAAGTCACCATCCAGTACGAAGATGGAAAACCAAAACGTGTGGA
TACAGTGGTGATTTCTACCCAACACAAACCTGGTGTCACTCATAAACAAATCGAAGAAGCAGTCATTGAAGAATGTATCA
AAAAAATGATACCAAAAGAACTTCTTGTGAACACTCGTTATTTCATCAACCCAACTGGTAAGTTTGAAATTGGTGGTCCA
CATGGTGATACTGGTCTCACAGGTCGTAAGATCATTGTGGATACATACGGTGGAATGGGTCGTCATGGTGGTGGGGCTTT
CTCTGGAAAGGATCCATCAAAAGTAGACCGTTCCGCTGCTTACATGGGCCGTTACATTGCAAAAAACGTAGTCGCAGCAG
GTCTTGCACATAAATGTGAAGTCCAGTTGGCATACGCTATTGGTGTGGCACAGCCTGTATCCGTTCTTGTGGATACTTTT
GGAACAGGAACCATTTCTGATGAAGAAATTGCAAAACGAGTTCTTGCCAACTTCAAACTCACTCCAAAAGGGATCGTAGA
AGGTTTAGATCTTCTTGGAAAGGGAAGAACTTACCAAGAAACAGCAGCATATGGTCACTTCGGAAGAACAGGAAGTACAT
TCACTTGGGAAAAAACTGACAAAGCAGACGCTTTGAAAAAAGGATAA

Upstream 100 bases:

>100_bases
AATGCGGACTCCCTCGAGAGCTATGTGCGTGGACTCTTTCCTGACAATTCTATTGACGTACCACCTAAAAACGAAACTTT
AACCAAGTAAGGTAAAAAAT

Downstream 100 bases:

>100_bases
TTATGGGAGCACCTAGCCAATCCACAGCGGACAAAAAAGCAACAAGAGATGCATATGGCGAAGCCTTAGTTGAGTTAGGT
GCATCTAGACAAGATGTGGT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSSLKNYIFTSESVSEGHPDKVCDQISDAILDAYLAQDPKSRVACETLVTTNLVVIAGEITSKGKVDTQEIARDVIRKIG
YNDINMYFDADFAVVASHVHAQSPDIAQGVNEGEGLHTEQGAGDQGLMFGFAIAETPELMPAPLYYSHKLLEHLAELRHT
NKIDWLRPDAKSQVTIQYEDGKPKRVDTVVISTQHKPGVTHKQIEEAVIEECIKKMIPKELLVNTRYFINPTGKFEIGGP
HGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVAAGLAHKCEVQLAYAIGVAQPVSVLVDTF
GTGTISDEEIAKRVLANFKLTPKGIVEGLDLLGKGRTYQETAAYGHFGRTGSTFTWEKTDKADALKKG

Sequences:

>Translated_388_residues
MSSLKNYIFTSESVSEGHPDKVCDQISDAILDAYLAQDPKSRVACETLVTTNLVVIAGEITSKGKVDTQEIARDVIRKIG
YNDINMYFDADFAVVASHVHAQSPDIAQGVNEGEGLHTEQGAGDQGLMFGFAIAETPELMPAPLYYSHKLLEHLAELRHT
NKIDWLRPDAKSQVTIQYEDGKPKRVDTVVISTQHKPGVTHKQIEEAVIEECIKKMIPKELLVNTRYFINPTGKFEIGGP
HGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVAAGLAHKCEVQLAYAIGVAQPVSVLVDTF
GTGTISDEEIAKRVLANFKLTPKGIVEGLDLLGKGRTYQETAAYGHFGRTGSTFTWEKTDKADALKKG
>Mature_387_residues
SSLKNYIFTSESVSEGHPDKVCDQISDAILDAYLAQDPKSRVACETLVTTNLVVIAGEITSKGKVDTQEIARDVIRKIGY
NDINMYFDADFAVVASHVHAQSPDIAQGVNEGEGLHTEQGAGDQGLMFGFAIAETPELMPAPLYYSHKLLEHLAELRHTN
KIDWLRPDAKSQVTIQYEDGKPKRVDTVVISTQHKPGVTHKQIEEAVIEECIKKMIPKELLVNTRYFINPTGKFEIGGPH
GDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVAAGLAHKCEVQLAYAIGVAQPVSVLVDTFG
TGTISDEEIAKRVLANFKLTPKGIVEGLDLLGKGRTYQETAAYGHFGRTGSTFTWEKTDKADALKKG

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=377, Percent_Identity=52.2546419098143, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI4557737, Length=376, Percent_Identity=52.1276595744681, Blast_Score=361, Evalue=1e-100,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=60.15625, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI25145633, Length=384, Percent_Identity=54.4270833333333, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17538498, Length=377, Percent_Identity=54.9071618037135, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=54.6632124352332, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17538494, Length=381, Percent_Identity=54.3307086614173, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565905, Length=381, Percent_Identity=49.0813648293963, Blast_Score=312, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI25145635, Length=334, Percent_Identity=52.0958083832335, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI32565911, Length=331, Percent_Identity=52.2658610271903, Blast_Score=304, Evalue=7e-83,
Organism=Caenorhabditis elegans, GI32565903, Length=304, Percent_Identity=51.6447368421053, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI32565909, Length=77, Percent_Identity=66.2337662337662, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32565907, Length=43, Percent_Identity=74.4186046511628, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320710, Length=386, Percent_Identity=52.8497409326425, Blast_Score=388, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=53.4391534391534, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45552159, Length=377, Percent_Identity=51.1936339522546, Blast_Score=347, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24580529, Length=377, Percent_Identity=51.1936339522546, Blast_Score=347, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24580537, Length=377, Percent_Identity=51.1936339522546, Blast_Score=347, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24580533, Length=377, Percent_Identity=51.1936339522546, Blast_Score=347, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24580535, Length=377, Percent_Identity=51.1936339522546, Blast_Score=347, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24580531, Length=377, Percent_Identity=51.1936339522546, Blast_Score=347, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24580527, Length=377, Percent_Identity=50.6631299734748, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI24580525, Length=377, Percent_Identity=50.6631299734748, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI24580541, Length=378, Percent_Identity=48.6772486772487, Blast_Score=322, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=50.8196721311475, Blast_Score=125, Evalue=5e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42182; Mature: 42051

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLKNYIFTSESVSEGHPDKVCDQISDAILDAYLAQDPKSRVACETLVTTNLVVIAGEI
CCCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECC
TSKGKVDTQEIARDVIRKIGYNDINMYFDADFAVVASHVHAQSPDIAQGVNEGEGLHTEQ
CCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHHCCCCCCCCCCCC
GAGDQGLMFGFAIAETPELMPAPLYYSHKLLEHLAELRHTNKIDWLRPDAKSQVTIQYED
CCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
GKPKRVDTVVISTQHKPGVTHKQIEEAVIEECIKKMIPKELLVNTRYFINPTGKFEIGGP
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCEEEECCCCCEEECCC
HGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVAAGLAHKCE
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHE
VQLAYAIGVAQPVSVLVDTFGTGTISDEEIAKRVLANFKLTPKGIVEGLDLLGKGRTYQE
EEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHH
TAAYGHFGRTGSTFTWEKTDKADALKKG
HHHHCCCCCCCCEEEECCCCHHHHHCCC
>Mature Secondary Structure 
SSLKNYIFTSESVSEGHPDKVCDQISDAILDAYLAQDPKSRVACETLVTTNLVVIAGEI
CCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECC
TSKGKVDTQEIARDVIRKIGYNDINMYFDADFAVVASHVHAQSPDIAQGVNEGEGLHTEQ
CCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHHCCCCCCCCCCCC
GAGDQGLMFGFAIAETPELMPAPLYYSHKLLEHLAELRHTNKIDWLRPDAKSQVTIQYED
CCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
GKPKRVDTVVISTQHKPGVTHKQIEEAVIEECIKKMIPKELLVNTRYFINPTGKFEIGGP
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCEEEECCCCCEEECCC
HGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVAAGLAHKCE
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHE
VQLAYAIGVAQPVSVLVDTFGTGTISDEEIAKRVLANFKLTPKGIVEGLDLLGKGRTYQE
EEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHH
TAAYGHFGRTGSTFTWEKTDKADALKKG
HHHHCCCCCCCCEEEECCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA