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The map label for this gene is ptsN [H]

Identifier: 183222189

GI number: 183222189

Start: 2929776

End: 2930237

Strand: Reverse

Name: ptsN [H]

Synonym: LEPBI_I2838

Alternate gene names: 183222189

Gene position: 2930237-2929776 (Counterclockwise)

Preceding gene: 183222190

Following gene: 183222188

Centisome position: 81.4

GC content: 36.8

Gene sequence:

>462_bases
ATGAATCAACTTCTGGAGATTCTGGACCCTAAAAATATCATTTTCGACTTCAAAGCGTCAACTAAAGAAGATGCAATTCG
GAAAATGATTTCTCATATGGTCGCCACACAAACATTAGATGCTGTGCATGAAGAAGAAACTGTTTCTTCTTTGATGAACC
GTGAAAAATCAATGTCAACAGGTATTGGAAGTGGAGTTGCGATCCCACATTGTTCTGTACATTATGTGAACGAACTCAAG
TGTGCAATGGCGATTTCCCCGAATGGAATTGATTTTGATGCCCTTGATCACGGACTCGTTCAAATATTTATCATGTTAAT
TGTTCCCAAAAACAAATTTCAAGATCACATTAAAACCTTGGCCTTAATCGCAAAAACCTTAAACATACCCGAAGAAAGAG
AGAAACTCATCAAAGCCAAAAATTTCGAAGAAATCCAAAAGGCATTCCTCTCAAAAAGTTAA

Upstream 100 bases:

>100_bases
TATGGGTAGTGATAGTTTTTTTTCTTTTAGAATCACTATTTGTGAGGGTTTTTCTTGGAAAATAAGTCGAGTTTGGAAAT
GTGGGAGTAAGTAATACCAA

Downstream 100 bases:

>100_bases
CGCTGTGAAGTCGGAAGTATTCCGTTTCCTTTATTATCTTTTGCTTTTATCACTTGTGAGTAGTTATGTATCCGAGTTTC
ATACCAAGGATAAAATATAT

Product: PTS system, IIA component

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MNQLLEILDPKNIIFDFKASTKEDAIRKMISHMVATQTLDAVHEEETVSSLMNREKSMSTGIGSGVAIPHCSVHYVNELK
CAMAISPNGIDFDALDHGLVQIFIMLIVPKNKFQDHIKTLALIAKTLNIPEEREKLIKAKNFEEIQKAFLSKS

Sequences:

>Translated_153_residues
MNQLLEILDPKNIIFDFKASTKEDAIRKMISHMVATQTLDAVHEEETVSSLMNREKSMSTGIGSGVAIPHCSVHYVNELK
CAMAISPNGIDFDALDHGLVQIFIMLIVPKNKFQDHIKTLALIAKTLNIPEEREKLIKAKNFEEIQKAFLSKS
>Mature_153_residues
MNQLLEILDPKNIIFDFKASTKEDAIRKMISHMVATQTLDAVHEEETVSSLMNREKSMSTGIGSGVAIPHCSVHYVNELK
CAMAISPNGIDFDALDHGLVQIFIMLIVPKNKFQDHIKTLALIAKTLNIPEEREKLIKAKNFEEIQKAFLSKS

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 17164; Mature: 17164

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLLEILDPKNIIFDFKASTKEDAIRKMISHMVATQTLDAVHEEETVSSLMNREKSMST
CCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIGSGVAIPHCSVHYVNELKCAMAISPNGIDFDALDHGLVQIFIMLIVPKNKFQDHIKTL
CCCCCCCCCCCHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ALIAKTLNIPEEREKLIKAKNFEEIQKAFLSKS
HHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MNQLLEILDPKNIIFDFKASTKEDAIRKMISHMVATQTLDAVHEEETVSSLMNREKSMST
CCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIGSGVAIPHCSVHYVNELKCAMAISPNGIDFDALDHGLVQIFIMLIVPKNKFQDHIKTL
CCCCCCCCCCCHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ALIAKTLNIPEEREKLIKAKNFEEIQKAFLSKS
HHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]