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The map label for this gene is dusA [H]

Identifier: 183221906

GI number: 183221906

Start: 2634292

End: 2635263

Strand: Reverse

Name: dusA [H]

Synonym: LEPBI_I2545

Alternate gene names: 183221906

Gene position: 2635263-2634292 (Counterclockwise)

Preceding gene: 183221907

Following gene: 183221905

Centisome position: 73.21

GC content: 44.14

Gene sequence:

>972_bases
TTGACAGACCCAGTCCCATCGCACCGCATTTCTGTTGCTCCGATGATGGACTGGACCGACAGGCATTTTCGTTATTTTAT
CCGACTTATCTCCAAACATGCGTTACTGTATACGGAGATGGTGACAACAGGTGCCATCCTTCGTGGCAAAGACAACCACA
GATACTTAGATTTTTCCAAGGAAGAACACCCCATTGCTCTCCAGTTAGGTGGTGACTCACCCAAAGCTCTAGCTGAATGT
GCCAAAATTGGGGAAGACTATGGGTATGATGAAATCAATCTGAATGTTGGTTGCCCTTCGGACCGGGTACAGAGTGGCAG
TTTTGGGGCTTGTCTCATGAAAGAACCAGATCTTGTGGCGGAGATGGTAGCTTTTTGTAAGTCGAAGGTCAAAATTCCTG
TGACCGTCAAACATCGAATTGGTGTGAATGGAAAAGAAAGTTACGAAGACCTTCACCAATTTGTATCCAAAATCCAAAAA
GCGGGTGTGGACCATTGTATCGTCCATGCAAGGATTGCGATTTTAGAGGGACTTTCTCCCAAAGAAAATCGAACCATTCC
ACCTCTCCGTTATGAAGATGTCTATCGTTTGAAATCAGATTTTCCCAATCTGCCGATCACCATCAATGGAGGGATCAAAA
CGCACGCAGAAATTAAAACTCACCTAACAAAAGCAGATGGAGTGATGGTCGGGCGTGCGGCATACGACAATCCATTTTTA
TTTGCTGATGTGGATTCCCTCTACTTTGGTTCCAAGGAGGGAACAATCACGAGAGAATCCATTTTACAAAAAATGATTCC
ATACGTACAATCTGTACGAATGAGTGGCGGAAAAGTCAGCCACATACTTCGGCATATTTTGGGATTGTACCACGGCGAAA
AAGGAGCAAGGGAATTTCGAAGGTATTTCACTAATGGAATGCACCTAACATCAGCCACTGAGTCCATTTTAGAATCCTAT
TTACAGCGTTAA

Upstream 100 bases:

>100_bases
GCTTCTGAAGAAATCCATCGAGGGGTTCCGACAGAAATTGCCTCGGTATTAAGCCTTCCATCGCGAGAGGAACACCCACA
AGAAGCTTAAGGAGATCCCT

Downstream 100 bases:

>100_bases
GGATCCGAAGGGCTTGGTCTTTTGGATTCGTCGCAAAGACGAAGACAAAAGACGGAAGCGTGAGCGGACCCCGAAGGAGC
CTGGCCCATGTCTCGGAAAA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTDPVPSHRISVAPMMDWTDRHFRYFIRLISKHALLYTEMVTTGAILRGKDNHRYLDFSKEEHPIALQLGGDSPKALAEC
AKIGEDYGYDEINLNVGCPSDRVQSGSFGACLMKEPDLVAEMVAFCKSKVKIPVTVKHRIGVNGKESYEDLHQFVSKIQK
AGVDHCIVHARIAILEGLSPKENRTIPPLRYEDVYRLKSDFPNLPITINGGIKTHAEIKTHLTKADGVMVGRAAYDNPFL
FADVDSLYFGSKEGTITRESILQKMIPYVQSVRMSGGKVSHILRHILGLYHGEKGAREFRRYFTNGMHLTSATESILESY
LQR

Sequences:

>Translated_323_residues
MTDPVPSHRISVAPMMDWTDRHFRYFIRLISKHALLYTEMVTTGAILRGKDNHRYLDFSKEEHPIALQLGGDSPKALAEC
AKIGEDYGYDEINLNVGCPSDRVQSGSFGACLMKEPDLVAEMVAFCKSKVKIPVTVKHRIGVNGKESYEDLHQFVSKIQK
AGVDHCIVHARIAILEGLSPKENRTIPPLRYEDVYRLKSDFPNLPITINGGIKTHAEIKTHLTKADGVMVGRAAYDNPFL
FADVDSLYFGSKEGTITRESILQKMIPYVQSVRMSGGKVSHILRHILGLYHGEKGAREFRRYFTNGMHLTSATESILESY
LQR
>Mature_322_residues
TDPVPSHRISVAPMMDWTDRHFRYFIRLISKHALLYTEMVTTGAILRGKDNHRYLDFSKEEHPIALQLGGDSPKALAECA
KIGEDYGYDEINLNVGCPSDRVQSGSFGACLMKEPDLVAEMVAFCKSKVKIPVTVKHRIGVNGKESYEDLHQFVSKIQKA
GVDHCIVHARIAILEGLSPKENRTIPPLRYEDVYRLKSDFPNLPITINGGIKTHAEIKTHLTKADGVMVGRAAYDNPFLF
ADVDSLYFGSKEGTITRESILQKMIPYVQSVRMSGGKVSHILRHILGLYHGEKGAREFRRYFTNGMHLTSATESILESYL
QR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=240, Percent_Identity=30, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI40807366, Length=294, Percent_Identity=25.5102040816327, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI8923374, Length=297, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI145693211, Length=322, Percent_Identity=54.9689440993789, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI1789660, Length=337, Percent_Identity=24.6290801186944, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788462, Length=243, Percent_Identity=27.1604938271605, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=235, Percent_Identity=27.6595744680851, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=249, Percent_Identity=24.0963855421687, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=237, Percent_Identity=27.0042194092827, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323437, Length=247, Percent_Identity=24.2914979757085, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24580595, Length=313, Percent_Identity=24.2811501597444, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920448, Length=313, Percent_Identity=24.2811501597444, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921524, Length=303, Percent_Identity=23.4323432343234, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585320, Length=236, Percent_Identity=25, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36352; Mature: 36221

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPVPSHRISVAPMMDWTDRHFRYFIRLISKHALLYTEMVTTGAILRGKDNHRYLDFSK
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEECCCC
EEHPIALQLGGDSPKALAECAKIGEDYGYDEINLNVGCPSDRVQSGSFGACLMKEPDLVA
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCCCCEEECCCCHHHH
EMVAFCKSKVKIPVTVKHRIGVNGKESYEDLHQFVSKIQKAGVDHCIVHARIAILEGLSP
HHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
KENRTIPPLRYEDVYRLKSDFPNLPITINGGIKTHAEIKTHLTKADGVMVGRAAYDNPFL
CCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
FADVDSLYFGSKEGTITRESILQKMIPYVQSVRMSGGKVSHILRHILGLYHGEKGAREFR
EEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHH
RYFTNGMHLTSATESILESYLQR
HHHHCCCEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDPVPSHRISVAPMMDWTDRHFRYFIRLISKHALLYTEMVTTGAILRGKDNHRYLDFSK
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEECCCC
EEHPIALQLGGDSPKALAECAKIGEDYGYDEINLNVGCPSDRVQSGSFGACLMKEPDLVA
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCCCCEEECCCCHHHH
EMVAFCKSKVKIPVTVKHRIGVNGKESYEDLHQFVSKIQKAGVDHCIVHARIAILEGLSP
HHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
KENRTIPPLRYEDVYRLKSDFPNLPITINGGIKTHAEIKTHLTKADGVMVGRAAYDNPFL
CCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
FADVDSLYFGSKEGTITRESILQKMIPYVQSVRMSGGKVSHILRHILGLYHGEKGAREFR
EEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHH
RYFTNGMHLTSATESILESYLQR
HHHHCCCEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]