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The map label for this gene is amtB2 [C]

Identifier: 183221778

GI number: 183221778

Start: 2479489

End: 2480898

Strand: Reverse

Name: amtB2 [C]

Synonym: LEPBI_I2408

Alternate gene names: 183221778

Gene position: 2480898-2479489 (Counterclockwise)

Preceding gene: 183221782

Following gene: 183221777

Centisome position: 68.92

GC content: 42.27

Gene sequence:

>1410_bases
ATGGAAAGGATACATGGCAATATGAAACAATATTTCAAATCAATCGCCTTCCTTCTCCTGGTCGTTCCGATGTTCCTTTT
TGCAGATGAAGCTGCAACCGTCGTGAGCCCAGCAGAAGAAACAGCAAAAGCAATCCAAACATTAACTGTAGGTCTTGACA
CATTATGGGTGTTAGTTGCAGGTATGTTGGTGTTCTTTATGAATGCTGGGTTTGCATTAGTAGAATCTGGATTTGCACAA
TCGAAAAACACAGTTAACATTTTAGCAAAAAACTTCATCGTATTTGCTGCCGCTACATTCTCGTATTGGGCAATCGGTTG
GGGTTTGATGTTTGGTGATGGAACTCCATATTATGCGACAGAAGGAGTTTTCTTTTTAACTGGACTTGATAACTCACCTG
CACTTGGGGATGACTATAAAGGTGTTTATTCTTCAATGAATTGGACTGGTGTTCCTCTTCTTGCAAAATTTTTCTTCCAA
TTGGTATTCGCTGCAACTGCTGCAACGATTGTATCGGGAGCTGTTGCTGAACGAATTAAATTCCATTCCTTCTTAATTTT
CTCATTTATCCTTGTAGCTGTGATGTATCCTTTCACAGGTCACTGGGTATGGGGTGGCGGTTGGTTAGCAGGTCTTGGTT
TTCATGACTTTGCTGGATCTACCGTAGTACACTCAGTAGGTGGATGGGCGGCTCTAGCTGGTGCCATCGTACTTGGTGCT
AGAAAAGGGAAATTTTTACCAGATGGTCGTATCAAACCAATCCTTGGTCACAATATGACATCTGCGGCGCTCGGAACACT
CATCCTATGGCTAGGTTGGTTTGGATTTAACCCTGGTTCAACGATGGGAGTAGGTGATGGAAGTGTTATGGCGCACGTCA
TCGTAACTACAAATATCTCTGCTGCATTAGGTGCACTTGCATCTACTGTCACTGCATGGATCATTTTAAAAAAACCTGAT
TTAGGTATGATTCTAAACGGAACCCTCGCTGGGCTTGTTGGGATTACAGCACCTTGTGCTATCGTAAGTCCAACTTCAGC
AGCAATCATCGGCGCTGTGTCAGGTGTACTTGTGGTTCTGTCCGTATTATTTTTTGATAAAATGAAAATTGATGACCCTG
TTGGAGCAACATCCGTTCACTTAGTATGTGGTATTTGGGGAACTCTTGCAGTTGCCATTTTTGGATACGAAGGATCTCCT
GCTGGAGTCGACGTTCCATCTTTCTTAACACAACTTTATGGAATTTTAGCAATCGGTGGATTTACATTTGTTGTTTCCTT
CATATTGTGGTTTGTATTAAAACTTGCTGGTGGAATCCGAGTGAGTGAAGAAGAAGAATTAAGTGGATTGGATCTAGGGG
AACACGGAGCAGAAGCTTACCCTGATTTTAATATCCGAGTTCGTGGTTAA

Upstream 100 bases:

>100_bases
CAATTTGGAGGGTTTATAAGCAACCTTTCGTTTTTTTCCTGGAAAAATTCCTAAATTTTGCCTGCGGGTACCAATCTTGC
ATCCTAATTCAGCAAATAAC

Downstream 100 bases:

>100_bases
GGGAATAGGAGTTTAACATTATGAAAATGATCATTGCAATCATTCAACCACATAAGTTGGAAGAAGTAAAAGCTGAGTTA
ACCAAAAACGAAATCTATCG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MERIHGNMKQYFKSIAFLLLVVPMFLFADEAATVVSPAEETAKAIQTLTVGLDTLWVLVAGMLVFFMNAGFALVESGFAQ
SKNTVNILAKNFIVFAAATFSYWAIGWGLMFGDGTPYYATEGVFFLTGLDNSPALGDDYKGVYSSMNWTGVPLLAKFFFQ
LVFAATAATIVSGAVAERIKFHSFLIFSFILVAVMYPFTGHWVWGGGWLAGLGFHDFAGSTVVHSVGGWAALAGAIVLGA
RKGKFLPDGRIKPILGHNMTSAALGTLILWLGWFGFNPGSTMGVGDGSVMAHVIVTTNISAALGALASTVTAWIILKKPD
LGMILNGTLAGLVGITAPCAIVSPTSAAIIGAVSGVLVVLSVLFFDKMKIDDPVGATSVHLVCGIWGTLAVAIFGYEGSP
AGVDVPSFLTQLYGILAIGGFTFVVSFILWFVLKLAGGIRVSEEEELSGLDLGEHGAEAYPDFNIRVRG

Sequences:

>Translated_469_residues
MERIHGNMKQYFKSIAFLLLVVPMFLFADEAATVVSPAEETAKAIQTLTVGLDTLWVLVAGMLVFFMNAGFALVESGFAQ
SKNTVNILAKNFIVFAAATFSYWAIGWGLMFGDGTPYYATEGVFFLTGLDNSPALGDDYKGVYSSMNWTGVPLLAKFFFQ
LVFAATAATIVSGAVAERIKFHSFLIFSFILVAVMYPFTGHWVWGGGWLAGLGFHDFAGSTVVHSVGGWAALAGAIVLGA
RKGKFLPDGRIKPILGHNMTSAALGTLILWLGWFGFNPGSTMGVGDGSVMAHVIVTTNISAALGALASTVTAWIILKKPD
LGMILNGTLAGLVGITAPCAIVSPTSAAIIGAVSGVLVVLSVLFFDKMKIDDPVGATSVHLVCGIWGTLAVAIFGYEGSP
AGVDVPSFLTQLYGILAIGGFTFVVSFILWFVLKLAGGIRVSEEEELSGLDLGEHGAEAYPDFNIRVRG
>Mature_469_residues
MERIHGNMKQYFKSIAFLLLVVPMFLFADEAATVVSPAEETAKAIQTLTVGLDTLWVLVAGMLVFFMNAGFALVESGFAQ
SKNTVNILAKNFIVFAAATFSYWAIGWGLMFGDGTPYYATEGVFFLTGLDNSPALGDDYKGVYSSMNWTGVPLLAKFFFQ
LVFAATAATIVSGAVAERIKFHSFLIFSFILVAVMYPFTGHWVWGGGWLAGLGFHDFAGSTVVHSVGGWAALAGAIVLGA
RKGKFLPDGRIKPILGHNMTSAALGTLILWLGWFGFNPGSTMGVGDGSVMAHVIVTTNISAALGALASTVTAWIILKKPD
LGMILNGTLAGLVGITAPCAIVSPTSAAIIGAVSGVLVVLSVLFFDKMKIDDPVGATSVHLVCGIWGTLAVAIFGYEGSP
AGVDVPSFLTQLYGILAIGGFTFVVSFILWFVLKLAGGIRVSEEEELSGLDLGEHGAEAYPDFNIRVRG

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=416, Percent_Identity=33.6538461538462, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17550182, Length=435, Percent_Identity=34.7126436781609, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI32564042, Length=428, Percent_Identity=35.5140186915888, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17550180, Length=443, Percent_Identity=34.0857787810384, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI133931100, Length=431, Percent_Identity=33.4106728538283, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6324187, Length=450, Percent_Identity=29.3333333333333, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321559, Length=426, Percent_Identity=27.4647887323944, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325396, Length=426, Percent_Identity=27.2300469483568, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078314, Length=423, Percent_Identity=38.0614657210402, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 49546; Mature: 49546

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIHGNMKQYFKSIAFLLLVVPMFLFADEAATVVSPAEETAKAIQTLTVGLDTLWVLVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GMLVFFMNAGFALVESGFAQSKNTVNILAKNFIVFAAATFSYWAIGWGLMFGDGTPYYAT
HHHHHHHCCCHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEE
EGVFFLTGLDNSPALGDDYKGVYSSMNWTGVPLLAKFFFQLVFAATAATIVSGAVAERIK
CCEEEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHSFLIFSFILVAVMYPFTGHWVWGGGWLAGLGFHDFAGSTVVHSVGGWAALAGAIVLGA
HHHHHHHHHHHHHHHCCCCCCEEECCCCEECCCCHHCCCCHHHHHCCHHHHHHHHHHEEC
RKGKFLPDGRIKPILGHNMTSAALGTLILWLGWFGFNPGSTMGVGDGSVMAHVIVTTNIS
CCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCHH
AALGALASTVTAWIILKKPDLGMILNGTLAGLVGITAPCAIVSPTSAAIIGAVSGVLVVL
HHHHHHHHHHHEEEEEECCCCCEEECHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHH
SVLFFDKMKIDDPVGATSVHLVCGIWGTLAVAIFGYEGSPAGVDVPSFLTQLYGILAIGG
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
FTFVVSFILWFVLKLAGGIRVSEEEELSGLDLGEHGAEAYPDFNIRVRG
HHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MERIHGNMKQYFKSIAFLLLVVPMFLFADEAATVVSPAEETAKAIQTLTVGLDTLWVLVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GMLVFFMNAGFALVESGFAQSKNTVNILAKNFIVFAAATFSYWAIGWGLMFGDGTPYYAT
HHHHHHHCCCHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEE
EGVFFLTGLDNSPALGDDYKGVYSSMNWTGVPLLAKFFFQLVFAATAATIVSGAVAERIK
CCEEEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHSFLIFSFILVAVMYPFTGHWVWGGGWLAGLGFHDFAGSTVVHSVGGWAALAGAIVLGA
HHHHHHHHHHHHHHHCCCCCCEEECCCCEECCCCHHCCCCHHHHHCCHHHHHHHHHHEEC
RKGKFLPDGRIKPILGHNMTSAALGTLILWLGWFGFNPGSTMGVGDGSVMAHVIVTTNIS
CCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCHH
AALGALASTVTAWIILKKPDLGMILNGTLAGLVGITAPCAIVSPTSAAIIGAVSGVLVVL
HHHHHHHHHHHEEEEEECCCCCEEECHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHH
SVLFFDKMKIDDPVGATSVHLVCGIWGTLAVAIFGYEGSPAGVDVPSFLTQLYGILAIGG
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
FTFVVSFILWFVLKLAGGIRVSEEEELSGLDLGEHGAEAYPDFNIRVRG
HHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]