Click here to switch to the map view.

The map label for this gene is glnK [H]

Identifier: 183221777

GI number: 183221777

Start: 2479124

End: 2479468

Strand: Reverse

Name: glnK [H]

Synonym: LEPBI_I2407

Alternate gene names: 183221777

Gene position: 2479468-2479124 (Counterclockwise)

Preceding gene: 183221778

Following gene: 183221774

Centisome position: 68.88

GC content: 35.07

Gene sequence:

>345_bases
ATGAAAATGATCATTGCAATCATTCAACCACATAAGTTGGAAGAAGTAAAAGCTGAGTTAACCAAAAACGAAATCTATCG
TTTGACAGTATCTGATGTTCAAGGTTACGGACAACAAAAAGGAAAAACAGAAGTTTTCCGTGGTCACGAATACACTGTAA
ATTTACTTAGAAAAGTAAGATTGGAAATAGCAGTCAACGATGAATTCGTAAAACCAACAGTGGATGCGATCTTAAAAGCA
GCAAAAAGTGGTGACGGTAAAATTGGTGATGGTAAAATTTTTATCACTCCATTAGAAGAAGTGATTCGAATCAGAACTGG
CGAAAAAGGCAAAAACGCCATTTAA

Upstream 100 bases:

>100_bases
AAGAAGAATTAAGTGGATTGGATCTAGGGGAACACGGAGCAGAAGCTTACCCTGATTTTAATATCCGAGTTCGTGGTTAA
GGGAATAGGAGTTTAACATT

Downstream 100 bases:

>100_bases
CATTCCTTTATCAAAGGAAAAATCGGAAAACGCGCGGGTGGGATACCACTCGTGCGTTTTTTTTTATTGAATGAAAATTT
CCAATGAATTCGTCCCTTCT

Product: nitrogen regulatory protein PII

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 114; Mature: 114

Protein sequence:

>114_residues
MKMIIAIIQPHKLEEVKAELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYTVNLLRKVRLEIAVNDEFVKPTVDAILKA
AKSGDGKIGDGKIFITPLEEVIRIRTGEKGKNAI

Sequences:

>Translated_114_residues
MKMIIAIIQPHKLEEVKAELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYTVNLLRKVRLEIAVNDEFVKPTVDAILKA
AKSGDGKIGDGKIFITPLEEVIRIRTGEKGKNAI
>Mature_114_residues
MKMIIAIIQPHKLEEVKAELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYTVNLLRKVRLEIAVNDEFVKPTVDAILKA
AKSGDGKIGDGKIFITPLEEVIRIRTGEKGKNAI

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=114, Percent_Identity=48.2456140350877, Blast_Score=110, Evalue=2e-26,
Organism=Escherichia coli, GI1788904, Length=114, Percent_Identity=50.8771929824561, Blast_Score=110, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12791; Mature: 12791

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMIIAIIQPHKLEEVKAELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYTVNLLRKVR
CCEEEEEECCCCHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCCCEECCCHHHHHHHHHHH
LEIAVNDEFVKPTVDAILKAAKSGDGKIGDGKIFITPLEEVIRIRTGEKGKNAI
HEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKMIIAIIQPHKLEEVKAELTKNEIYRLTVSDVQGYGQQKGKTEVFRGHEYTVNLLRKVR
CCEEEEEECCCCHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCCCEECCCHHHHHHHHHHH
LEIAVNDEFVKPTVDAILKAAKSGDGKIGDGKIFITPLEEVIRIRTGEKGKNAI
HEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]