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The map label for this gene is kinB [H]

Identifier: 183221467

GI number: 183221467

Start: 2178774

End: 2179928

Strand: Direct

Name: kinB [H]

Synonym: LEPBI_Ia2079

Alternate gene names: 183221467

Gene position: 2178774-2179928 (Clockwise)

Preceding gene: 183221466

Following gene: 183221468

Centisome position: 60.53

GC content: 36.71

Gene sequence:

>1155_bases
TTGTCAGAATTTATAAATCATGGAGACTCACATTCGATCGAAAACATCGAAAAGGTCGCAGAAAAAGCGAGAGAACTGGA
GGCCATTTATGATGTGGTGCAAGACCCTCTTGTACTCATCGATTCCGACTTTAATATCCAAAGGGCAAATTTAGCCACCA
TTCGATTCGCAAAAAACAATAAATATGATGAATTATTGGACCGAAAATGTTACGAGGTTTTATACCAACGAACAGACGTT
TGCCCATATTGTCCCAAAATCAATGTCAAATCCAAGGACAAAAATCAGTCTTATTCCACACCAATCACCCGTGAAATTTT
TTTCCGATCAGAAGATAAAAAACAAACCCTACTTTTGGAATTTTACCCCTACCCCAAACAAGAAGATTTGTTTTGGATGG
TGGAAAAAATATCAGATGTCACAAAACAACGAGACAAGGAAGAAGAGTCATTTCGAATGCGTAACCTTGCTTCACTTGGG
ATTTTAATCTCAGGTATTGCACATGAGTTAAACAATCCTCTAACGGGAATTAGTTTAACATTACAAAATTTAAAAGCAAA
TTGGCAAAACCAACCTCCCGAACAAATTGAAAAACGTTTGGATATGATTAAAAATGATATATCTCGAGCAGCAATCATTG
TCTCCGATATCATTTCATTTGCAAAAACAGACAAAGTCAAAGTCACACTCGGTGATATTGTCGAAACCATTACTCGCGCA
AAAGATACGGTCATTCGATTGTATCCTCATTTGAGTAAAAATATAAATTGGCGGATCACTTGTGATCACGAGTACCAATT
CCCTTTCCATCCAGGCAAGATGGAGCGCTTATTTATGAACTTATTTCGTAATTCTTTACAAGCATTCGATTATAGACCTG
GGGAAATTTCGATTGAGATACGTAAGACAAAAAACTGGCTTCATATTATTATCGAAGACAATGCGGGCGGAATTCCTGAC
TCCATCATCCAAAAAATTTTTGATCCATTTTTTACTAGTAACAAATCAGGAACAGGAACAGGGCTTGGGCTATCCATTTG
CCACTCTATTGTAAAAGAACATGATGGAAACATATCTGTCAAATCAGTAGAACAAAAGACAAGATTCACAATTTCTTTTC
CGCTCACAAATGATATTACGGAGCCAAATTCATGA

Upstream 100 bases:

>100_bases
TATAGCCAACTTGCTTCCATTAATGCCCTACAAAAATCAAGTGGCAATCCCAACCAAAAGAAGATGTTCAAACAACTCAA
CCAATCCTTAGGTTACTAAT

Downstream 100 bases:

>100_bases
AACAATCCATTTTGATTGTAGAAGACATTCATTCGATACGTGAAGCGATCATGGATCTCTTGAGTACCAAATTTAATGTG
TTTGGGGCGGAACATTTTGA

Product: histidine kinase sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSEFINHGDSHSIENIEKVAEKARELEAIYDVVQDPLVLIDSDFNIQRANLATIRFAKNNKYDELLDRKCYEVLYQRTDV
CPYCPKINVKSKDKNQSYSTPITREIFFRSEDKKQTLLLEFYPYPKQEDLFWMVEKISDVTKQRDKEEESFRMRNLASLG
ILISGIAHELNNPLTGISLTLQNLKANWQNQPPEQIEKRLDMIKNDISRAAIIVSDIISFAKTDKVKVTLGDIVETITRA
KDTVIRLYPHLSKNINWRITCDHEYQFPFHPGKMERLFMNLFRNSLQAFDYRPGEISIEIRKTKNWLHIIIEDNAGGIPD
SIIQKIFDPFFTSNKSGTGTGLGLSICHSIVKEHDGNISVKSVEQKTRFTISFPLTNDITEPNS

Sequences:

>Translated_384_residues
MSEFINHGDSHSIENIEKVAEKARELEAIYDVVQDPLVLIDSDFNIQRANLATIRFAKNNKYDELLDRKCYEVLYQRTDV
CPYCPKINVKSKDKNQSYSTPITREIFFRSEDKKQTLLLEFYPYPKQEDLFWMVEKISDVTKQRDKEEESFRMRNLASLG
ILISGIAHELNNPLTGISLTLQNLKANWQNQPPEQIEKRLDMIKNDISRAAIIVSDIISFAKTDKVKVTLGDIVETITRA
KDTVIRLYPHLSKNINWRITCDHEYQFPFHPGKMERLFMNLFRNSLQAFDYRPGEISIEIRKTKNWLHIIIEDNAGGIPD
SIIQKIFDPFFTSNKSGTGTGLGLSICHSIVKEHDGNISVKSVEQKTRFTISFPLTNDITEPNS
>Mature_383_residues
SEFINHGDSHSIENIEKVAEKARELEAIYDVVQDPLVLIDSDFNIQRANLATIRFAKNNKYDELLDRKCYEVLYQRTDVC
PYCPKINVKSKDKNQSYSTPITREIFFRSEDKKQTLLLEFYPYPKQEDLFWMVEKISDVTKQRDKEEESFRMRNLASLGI
LISGIAHELNNPLTGISLTLQNLKANWQNQPPEQIEKRLDMIKNDISRAAIIVSDIISFAKTDKVKVTLGDIVETITRAK
DTVIRLYPHLSKNINWRITCDHEYQFPFHPGKMERLFMNLFRNSLQAFDYRPGEISIEIRKTKNWLHIIIEDNAGGIPDS
IIQKIFDPFFTSNKSGTGTGLGLSICHSIVKEHDGNISVKSVEQKTRFTISFPLTNDITEPNS

Specific function: Member of the two-component regulatory system AlgB/KinB involved in regulation of alginate biosynthesis genes. KinB functions as a membrane-associated protein kinase that phosphorylates AlgB, probably in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=375, Percent_Identity=25.3333333333333, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790436, Length=233, Percent_Identity=28.3261802575107, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI145693157, Length=253, Percent_Identity=26.4822134387352, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790300, Length=259, Percent_Identity=25.8687258687259, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786783, Length=251, Percent_Identity=26.2948207171315, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=27.1186440677966, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788393, Length=227, Percent_Identity=22.4669603524229, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48994928, Length=337, Percent_Identity=23.7388724035608, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF08448 PAS_4 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44382; Mature: 44251

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFINHGDSHSIENIEKVAEKARELEAIYDVVQDPLVLIDSDFNIQRANLATIRFAKNN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCEEEEEEECCC
KYDELLDRKCYEVLYQRTDVCPYCPKINVKSKDKNQSYSTPITREIFFRSEDKKQTLLLE
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCEEEEE
FYPYPKQEDLFWMVEKISDVTKQRDKEEESFRMRNLASLGILISGIAHELNNPLTGISLT
ECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LQNLKANWQNQPPEQIEKRLDMIKNDISRAAIIVSDIISFAKTDKVKVTLGDIVETITRA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHH
KDTVIRLYPHLSKNINWRITCDHEYQFPFHPGKMERLFMNLFRNSLQAFDYRPGEISIEI
HHHHEEECHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEE
RKTKNWLHIIIEDNAGGIPDSIIQKIFDPFFTSNKSGTGTGLGLSICHSIVKEHDGNISV
EECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
KSVEQKTRFTISFPLTNDITEPNS
EEECCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SEFINHGDSHSIENIEKVAEKARELEAIYDVVQDPLVLIDSDFNIQRANLATIRFAKNN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCEEEEEEECCC
KYDELLDRKCYEVLYQRTDVCPYCPKINVKSKDKNQSYSTPITREIFFRSEDKKQTLLLE
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCEEEEE
FYPYPKQEDLFWMVEKISDVTKQRDKEEESFRMRNLASLGILISGIAHELNNPLTGISLT
ECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LQNLKANWQNQPPEQIEKRLDMIKNDISRAAIIVSDIISFAKTDKVKVTLGDIVETITRA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHH
KDTVIRLYPHLSKNINWRITCDHEYQFPFHPGKMERLFMNLFRNSLQAFDYRPGEISIEI
HHHHEEECHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEE
RKTKNWLHIIIEDNAGGIPDSIIQKIFDPFFTSNKSGTGTGLGLSICHSIVKEHDGNISV
EECCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
KSVEQKTRFTISFPLTNDITEPNS
EEECCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9218420 [H]