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The map label for this gene is leuA [H]

Identifier: 183221231

GI number: 183221231

Start: 1936625

End: 1938127

Strand: Direct

Name: leuA [H]

Synonym: LEPBI_I1845

Alternate gene names: 183221231

Gene position: 1936625-1938127 (Clockwise)

Preceding gene: 183221230

Following gene: 183221232

Centisome position: 53.8

GC content: 47.64

Gene sequence:

>1503_bases
ATGGAAGATTACGTACGGATATTTGATACAACTTTACGGGATGGAGAGCAGTGCCCTGGTGCTGCCATGAGTGAGGATGA
AAAAGTAGAAATTGCCCAGCACCTCGCTCGGATGAAAGTAGACATCATTGAAGCTGGATTCCCCGTATCCTCTCCCGTTC
AGTTCAAAGCGGTGGAAAGGATTGCCCGAGAGATTGAAGGTCCGACCATCTGTGGATTGGCTCGCGCCTTACGCCCAGAT
CTCGAAGCCGCTCGTGATGCCCTGAAACCCGCCAAATCCAAACGCATTCATACCTTTATCGCCTCCTCTCCCATCCATAT
GAAACACAAATTGGGTAAGTCCCCTACGGAAGTCCTCGAGATGGCACGGATAGCCGTGCGGATGGCAAAGGACTTTGTTT
CCGATGTTGAGTTTTCACCGGAAGATGCCACACGTTCCGAATGGGAATTCTTACGCGAGTTAGTCGAGGCTGTGATCGAA
GAAGGGGCAACCACCATCAATATCCCTGATACCGTTGGTTACACAACCCCACAAGAGTATATGGATCTCTTTCGTTTTTT
AAAAGAAAAAGTAAAGGGTGCAGACAAAGTCATATTTTCTGCCCACTGCCATAATGACCTAGGACTTGCCGTTGCCAATT
CCCTTGCCACAGTGCTTGCTGGTGGAAGACAAATTGAATGTACGATCAATGGAATCGGAGAACGTGCAGGCAATACAGCG
ATGGAAGAAGTGGTCATGGCACTAAAAACAAGAAAGGATACCTTTGGAGTCGAAACTAGAATTGATTCTACCCTCATCAC
ACGTGGTTCCCATTTGGTAAAGACCATCACGGGAATGGTGGTCCAACCGAATAAGGCCATCGTCGGTGCCAATGCCTTCG
CCCATGAGTCTGGGATCCACCAAGATGGGGTACTCAAAAATCGACAAACCTATGAAATCATGACCCCGGAATCTGTGGGA
TTAAAATCCAATCGAATGGTCCTTGGCCGCCATTCCGGAAGGGCTGGGTTCAAAGATCGGATTGTCCGAATGGGATTTGA
TCCAAAACCAGAAGAAATTGACAATGCCTACAATCGGTTTCTGGAAATTGCAGATCTTAAAAAAGAAGTTTTTGATGAGG
ACATCGCGGCTCTCTTCCAAGCAGAAATCAGTCGATCCCAAGTCGATGAAACCCACCGATTGCTCTCCTTTGAACAAAAT
ACTGGTTCGGACAAGACTCCCTTTGCAAAAGTCACCTTACAAACGAATGGTGAAACCAAAGTGGGTGAAGCAAAAGGGGA
TGGTCCTGTCGACAGTATCTTTAAGGCGATCAATTCCGTAACGGGTCTATCTCCCCTTTTGTCTCGGCTTGTGATCTCTC
CTGTCACGGAAGGCACAGATGCAATGGCAGAAGCTTCCGTCACTTTGGAGTCAGGCGAAAGGCGAGTCGTAGGAAAAGGA
GATTCCACCGATATCATTGAAGCTTGTGCCAAAGCCTATATCAATGCATTGAACCGGTTGTAA

Upstream 100 bases:

>100_bases
AACCCATTCCCATAGACAAATCAGGGGAACCATACCGCTGGATCACAGGTGCCTTCCATACTCCTTGACAGAGTTCGGGG
AAACTATGATCTGGAAGGAG

Downstream 100 bases:

>100_bases
ATCATGGATTCTTCGAAACAAAACTTCAATCCAGAATCGTTCATTCGAAAAGAACTCATTTCGTTTTCTCCATATACACC
AGGAGAACAACCAGCTCTTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MEDYVRIFDTTLRDGEQCPGAAMSEDEKVEIAQHLARMKVDIIEAGFPVSSPVQFKAVERIAREIEGPTICGLARALRPD
LEAARDALKPAKSKRIHTFIASSPIHMKHKLGKSPTEVLEMARIAVRMAKDFVSDVEFSPEDATRSEWEFLRELVEAVIE
EGATTINIPDTVGYTTPQEYMDLFRFLKEKVKGADKVIFSAHCHNDLGLAVANSLATVLAGGRQIECTINGIGERAGNTA
MEEVVMALKTRKDTFGVETRIDSTLITRGSHLVKTITGMVVQPNKAIVGANAFAHESGIHQDGVLKNRQTYEIMTPESVG
LKSNRMVLGRHSGRAGFKDRIVRMGFDPKPEEIDNAYNRFLEIADLKKEVFDEDIAALFQAEISRSQVDETHRLLSFEQN
TGSDKTPFAKVTLQTNGETKVGEAKGDGPVDSIFKAINSVTGLSPLLSRLVISPVTEGTDAMAEASVTLESGERRVVGKG
DSTDIIEACAKAYINALNRL

Sequences:

>Translated_500_residues
MEDYVRIFDTTLRDGEQCPGAAMSEDEKVEIAQHLARMKVDIIEAGFPVSSPVQFKAVERIAREIEGPTICGLARALRPD
LEAARDALKPAKSKRIHTFIASSPIHMKHKLGKSPTEVLEMARIAVRMAKDFVSDVEFSPEDATRSEWEFLRELVEAVIE
EGATTINIPDTVGYTTPQEYMDLFRFLKEKVKGADKVIFSAHCHNDLGLAVANSLATVLAGGRQIECTINGIGERAGNTA
MEEVVMALKTRKDTFGVETRIDSTLITRGSHLVKTITGMVVQPNKAIVGANAFAHESGIHQDGVLKNRQTYEIMTPESVG
LKSNRMVLGRHSGRAGFKDRIVRMGFDPKPEEIDNAYNRFLEIADLKKEVFDEDIAALFQAEISRSQVDETHRLLSFEQN
TGSDKTPFAKVTLQTNGETKVGEAKGDGPVDSIFKAINSVTGLSPLLSRLVISPVTEGTDAMAEASVTLESGERRVVGKG
DSTDIIEACAKAYINALNRL
>Mature_500_residues
MEDYVRIFDTTLRDGEQCPGAAMSEDEKVEIAQHLARMKVDIIEAGFPVSSPVQFKAVERIAREIEGPTICGLARALRPD
LEAARDALKPAKSKRIHTFIASSPIHMKHKLGKSPTEVLEMARIAVRMAKDFVSDVEFSPEDATRSEWEFLRELVEAVIE
EGATTINIPDTVGYTTPQEYMDLFRFLKEKVKGADKVIFSAHCHNDLGLAVANSLATVLAGGRQIECTINGIGERAGNTA
MEEVVMALKTRKDTFGVETRIDSTLITRGSHLVKTITGMVVQPNKAIVGANAFAHESGIHQDGVLKNRQTYEIMTPESVG
LKSNRMVLGRHSGRAGFKDRIVRMGFDPKPEEIDNAYNRFLEIADLKKEVFDEDIAALFQAEISRSQVDETHRLLSFEQN
TGSDKTPFAKVTLQTNGETKVGEAKGDGPVDSIFKAINSVTGLSPLLSRLVISPVTEGTDAMAEASVTLESGERRVVGKG
DSTDIIEACAKAYINALNRL

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=501, Percent_Identity=48.502994011976, Blast_Score=457, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320019, Length=404, Percent_Identity=27.7227722772277, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324682, Length=542, Percent_Identity=25.6457564575646, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=385, Percent_Identity=28.3116883116883, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=555, Percent_Identity=25.9459459459459, Blast_Score=130, Evalue=4e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 54805; Mature: 54805

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDYVRIFDTTLRDGEQCPGAAMSEDEKVEIAQHLARMKVDIIEAGFPVSSPVQFKAVER
CCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IAREIEGPTICGLARALRPDLEAARDALKPAKSKRIHTFIASSPIHMKHKLGKSPTEVLE
HHHHCCCCHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHH
MARIAVRMAKDFVSDVEFSPEDATRSEWEFLRELVEAVIEEGATTINIPDTVGYTTPQEY
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHH
MDLFRFLKEKVKGADKVIFSAHCHNDLGLAVANSLATVLAGGRQIECTINGIGERAGNTA
HHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHH
MEEVVMALKTRKDTFGVETRIDSTLITRGSHLVKTITGMVVQPNKAIVGANAFAHESGIH
HHHHHHHHHHCCHHCCCHHHCCHHHHHCCHHHHHHHHHHEECCCCEEEECCHHHHHCCCC
QDGVLKNRQTYEIMTPESVGLKSNRMVLGRHSGRAGFKDRIVRMGFDPKPEEIDNAYNRF
CCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHH
LEIADLKKEVFDEDIAALFQAEISRSQVDETHRLLSFEQNTGSDKTPFAKVTLQTNGETK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCC
VGEAKGDGPVDSIFKAINSVTGLSPLLSRLVISPVTEGTDAMAEASVTLESGERRVVGKG
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHEEEEECCCEEEECCC
DSTDIIEACAKAYINALNRL
CCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEDYVRIFDTTLRDGEQCPGAAMSEDEKVEIAQHLARMKVDIIEAGFPVSSPVQFKAVER
CCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IAREIEGPTICGLARALRPDLEAARDALKPAKSKRIHTFIASSPIHMKHKLGKSPTEVLE
HHHHCCCCHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHH
MARIAVRMAKDFVSDVEFSPEDATRSEWEFLRELVEAVIEEGATTINIPDTVGYTTPQEY
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHH
MDLFRFLKEKVKGADKVIFSAHCHNDLGLAVANSLATVLAGGRQIECTINGIGERAGNTA
HHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHH
MEEVVMALKTRKDTFGVETRIDSTLITRGSHLVKTITGMVVQPNKAIVGANAFAHESGIH
HHHHHHHHHHCCHHCCCHHHCCHHHHHCCHHHHHHHHHHEECCCCEEEECCHHHHHCCCC
QDGVLKNRQTYEIMTPESVGLKSNRMVLGRHSGRAGFKDRIVRMGFDPKPEEIDNAYNRF
CCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHH
LEIADLKKEVFDEDIAALFQAEISRSQVDETHRLLSFEQNTGSDKTPFAKVTLQTNGETK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCC
VGEAKGDGPVDSIFKAINSVTGLSPLLSRLVISPVTEGTDAMAEASVTLESGERRVVGKG
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHEEEEECCCEEEECCC
DSTDIIEACAKAYINALNRL
CCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA