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The map label for this gene is prfC [H]

Identifier: 183221192

GI number: 183221192

Start: 1885791

End: 1887380

Strand: Reverse

Name: prfC [H]

Synonym: LEPBI_I1806

Alternate gene names: 183221192

Gene position: 1887380-1885791 (Counterclockwise)

Preceding gene: 183221195

Following gene: 183221187

Centisome position: 52.43

GC content: 40.63

Gene sequence:

>1590_bases
ATGTCCCCTGAAATCATAGAAAAAGAAGTCAATCGCAGGAAAACATTCGCAATCATTGCGCACCCGGATGCCGGGAAAAC
CACCCTGACTGAAAAATTACTCCTCTACGGAGGTGCCATCCAACTGGCAGGGGCCGTGAAAGCCAAAAAAGAAGGGAAAT
CCGCTACCTCTGATTGGATGGCGATGGAGAAGGAAAGAGGGATTTCCATCACATCTGCTGCCTTACAATTTGAATATAAA
AACCATATCCTCAATTTACTCGACACTCCAGGCCACGAAGACTTTTCGGAAGACACCTACAGAACTTTGATGGCGGCAGA
TACCGCCGTCATGGTGTTAGATGCTGGAAAAGGAGTGGAACCACAAACCATCAAATTATTCCGAGTTTGTCGGGATCGTG
GGATTCCCATCATCACTTTTATCAATAAAATGGATCGTCCGACAAAAGATTTGTATGCGCTCTTGGATGAAATCGAAAAA
GTTTTGGGCATCAAAGCCGTTCCTGATGTTTGGCCTTTGGGAACAGGATTTGATTTTAAGGGAGTTTATGATTTAAGAGA
CCAACAATTGTATCTATTTGATCGGACTCCTGGAGGCAAACAAAAAGCGGGCTTTCGAATGGCAGGTCCCAATGATACAA
GTCTTGACGATCAGTTTGATGAGGAAATTGTAAAGGCATTCCGTGAACAAATTGATTTGGTGGAAAATGGAATTGGACAA
GTAGATAAAAATTCCTTTTTATTAGGCAAAGAAACTCCCGTATACTTCGGTTCGGCGGTAAATAACTTCGGAATCGAATT
GTTTTTAAATAAATTTTTGGAACTTGCCCCAGGTCCAGACCACATTCCACTCCGTGATGGAAACTATTTAGATCCAGTGA
ATTCCCCTTTCAGTGCATTTGTTTTTAAGGTGCAAGCCAATATGAACAAAGCCCATCGTGATCGAATTGCTTTTTTACGA
ATTTGTTCTGGCGTGTTTGAAAGAGGTTTGAATGTAAACCACAACCGTTTGGATAAACCTGTGAAACTGTCTTCGAGTTT
TGCTTTTTTTGGACAAGACCGAAACACAGTCGATTTAGCCTATCCTGGAGACATTATCGGTCTTGTGAATCCTGGGACCT
ATAAAATTGGTGATGTACTTTCTACTGGAAATACTCCTCCATTACGTCCACTTCCGAGTTTTGCTCCTGAGTTATTTGCG
ACCATTTCTTGTAAGGACACCTTACAATTAAAATCGTTTAAAAAAGGTTTAGACCAATTGGCAGAAGAAGGAATTTTGCA
TCTCTTCACCTCACGTACAATAGGCGGTGGTGTCCCTATCATTGGTGCCATGGGCAAATTACAATTTGAGGTCTTCCAAA
GGCGATTAAAGGATGAATATGGAGCCGACACTTCCATTCATATTTTACCGTATGGAATTTCCAGATGGGTGAAAAAAGAA
GATCGAAGTAAAATTCCATCCAATGCAAATTTAGTAGAAGATTTGTTTGGAAATATGGCTCTGTTATTTGATACAGAATG
GGATATGAATTATTTTCATAAAAACAATGAAGGAATTGAACTTTTAGACAATCCTCCCTTGGAAGAATGA

Upstream 100 bases:

>100_bases
GGGACATAATTCTAGAGTCAACCCTTCCTTGGGGAAAGGAACCTGATTCTTTCTTTATTCTCGACAGAATCGCCAATTTA
GGCAATATGGAATAGTCATT

Downstream 100 bases:

>100_bases
TTAAATGATATCAATACCAATCCAAGTGATGTCATCTAATAGGTCCTTTCCTTCTGAAAAAGATCTGATCTTGGAATCTA
ATTCTTGTTTTATGAAAGAC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSPEIIEKEVNRRKTFAIIAHPDAGKTTLTEKLLLYGGAIQLAGAVKAKKEGKSATSDWMAMEKERGISITSAALQFEYK
NHILNLLDTPGHEDFSEDTYRTLMAADTAVMVLDAGKGVEPQTIKLFRVCRDRGIPIITFINKMDRPTKDLYALLDEIEK
VLGIKAVPDVWPLGTGFDFKGVYDLRDQQLYLFDRTPGGKQKAGFRMAGPNDTSLDDQFDEEIVKAFREQIDLVENGIGQ
VDKNSFLLGKETPVYFGSAVNNFGIELFLNKFLELAPGPDHIPLRDGNYLDPVNSPFSAFVFKVQANMNKAHRDRIAFLR
ICSGVFERGLNVNHNRLDKPVKLSSSFAFFGQDRNTVDLAYPGDIIGLVNPGTYKIGDVLSTGNTPPLRPLPSFAPELFA
TISCKDTLQLKSFKKGLDQLAEEGILHLFTSRTIGGGVPIIGAMGKLQFEVFQRRLKDEYGADTSIHILPYGISRWVKKE
DRSKIPSNANLVEDLFGNMALLFDTEWDMNYFHKNNEGIELLDNPPLEE

Sequences:

>Translated_529_residues
MSPEIIEKEVNRRKTFAIIAHPDAGKTTLTEKLLLYGGAIQLAGAVKAKKEGKSATSDWMAMEKERGISITSAALQFEYK
NHILNLLDTPGHEDFSEDTYRTLMAADTAVMVLDAGKGVEPQTIKLFRVCRDRGIPIITFINKMDRPTKDLYALLDEIEK
VLGIKAVPDVWPLGTGFDFKGVYDLRDQQLYLFDRTPGGKQKAGFRMAGPNDTSLDDQFDEEIVKAFREQIDLVENGIGQ
VDKNSFLLGKETPVYFGSAVNNFGIELFLNKFLELAPGPDHIPLRDGNYLDPVNSPFSAFVFKVQANMNKAHRDRIAFLR
ICSGVFERGLNVNHNRLDKPVKLSSSFAFFGQDRNTVDLAYPGDIIGLVNPGTYKIGDVLSTGNTPPLRPLPSFAPELFA
TISCKDTLQLKSFKKGLDQLAEEGILHLFTSRTIGGGVPIIGAMGKLQFEVFQRRLKDEYGADTSIHILPYGISRWVKKE
DRSKIPSNANLVEDLFGNMALLFDTEWDMNYFHKNNEGIELLDNPPLEE
>Mature_528_residues
SPEIIEKEVNRRKTFAIIAHPDAGKTTLTEKLLLYGGAIQLAGAVKAKKEGKSATSDWMAMEKERGISITSAALQFEYKN
HILNLLDTPGHEDFSEDTYRTLMAADTAVMVLDAGKGVEPQTIKLFRVCRDRGIPIITFINKMDRPTKDLYALLDEIEKV
LGIKAVPDVWPLGTGFDFKGVYDLRDQQLYLFDRTPGGKQKAGFRMAGPNDTSLDDQFDEEIVKAFREQIDLVENGIGQV
DKNSFLLGKETPVYFGSAVNNFGIELFLNKFLELAPGPDHIPLRDGNYLDPVNSPFSAFVFKVQANMNKAHRDRIAFLRI
CSGVFERGLNVNHNRLDKPVKLSSSFAFFGQDRNTVDLAYPGDIIGLVNPGTYKIGDVLSTGNTPPLRPLPSFAPELFAT
ISCKDTLQLKSFKKGLDQLAEEGILHLFTSRTIGGGVPIIGAMGKLQFEVFQRRLKDEYGADTSIHILPYGISRWVKKED
RSKIPSNANLVEDLFGNMALLFDTEWDMNYFHKNNEGIELLDNPPLEE

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=481, Percent_Identity=28.2744282744283, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI19923640, Length=508, Percent_Identity=28.740157480315, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI25306283, Length=367, Percent_Identity=32.425068119891, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI25306287, Length=317, Percent_Identity=32.807570977918, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI157426893, Length=159, Percent_Identity=35.2201257861635, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI94966754, Length=145, Percent_Identity=32.4137931034483, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI4503483, Length=158, Percent_Identity=33.5443037974684, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI310132016, Length=121, Percent_Identity=33.0578512396694, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI310110807, Length=121, Percent_Identity=33.0578512396694, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI310123363, Length=121, Percent_Identity=33.0578512396694, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1790835, Length=485, Percent_Identity=46.5979381443299, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1789738, Length=488, Percent_Identity=27.6639344262295, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788922, Length=162, Percent_Identity=36.4197530864198, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI48994988, Length=181, Percent_Identity=33.1491712707182, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=499, Percent_Identity=25.4509018036072, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17556745, Length=177, Percent_Identity=40.6779661016949, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=141, Percent_Identity=36.1702127659575, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=173, Percent_Identity=31.2138728323699, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.0751879699248, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.0751879699248, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=488, Percent_Identity=26.4344262295082, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6322359, Length=517, Percent_Identity=27.0793036750484, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6323320, Length=167, Percent_Identity=30.5389221556886, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324707, Length=159, Percent_Identity=33.9622641509434, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320593, Length=159, Percent_Identity=33.9622641509434, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324166, Length=154, Percent_Identity=31.1688311688312, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=494, Percent_Identity=27.7327935222672, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI221458488, Length=500, Percent_Identity=25.8, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI78706572, Length=140, Percent_Identity=36.4285714285714, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585709, Length=167, Percent_Identity=32.9341317365269, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24585711, Length=158, Percent_Identity=32.9113924050633, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24585713, Length=158, Percent_Identity=32.9113924050633, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28574573, Length=151, Percent_Identity=29.8013245033113, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21357743, Length=172, Percent_Identity=27.3255813953488, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24656849, Length=142, Percent_Identity=30.9859154929577, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59078; Mature: 58947

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPEIIEKEVNRRKTFAIIAHPDAGKTTLTEKLLLYGGAIQLAGAVKAKKEGKSATSDWM
CCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
AMEKERGISITSAALQFEYKNHILNLLDTPGHEDFSEDTYRTLMAADTAVMVLDAGKGVE
HHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCC
PQTIKLFRVCRDRGIPIITFINKMDRPTKDLYALLDEIEKVLGIKAVPDVWPLGTGFDFK
HHHHHHHHHHHHCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GVYDLRDQQLYLFDRTPGGKQKAGFRMAGPNDTSLDDQFDEEIVKAFREQIDLVENGIGQ
CEEECCCCEEEEEECCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
VDKNSFLLGKETPVYFGSAVNNFGIELFLNKFLELAPGPDHIPLRDGNYLDPVNSPFSAF
CCCCCEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHE
VFKVQANMNKAHRDRIAFLRICSGVFERGLNVNHNRLDKPVKLSSSFAFFGQDRNTVDLA
EEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEECCCEEEECCCCCEEEEE
YPGDIIGLVNPGTYKIGDVLSTGNTPPLRPLPSFAPELFATISCKDTLQLKSFKKGLDQL
CCCCEEEEECCCCEEHHHHHHCCCCCCCCCCCCHHHHHHEEECCCHHHHHHHHHHHHHHH
AEEGILHLFTSRTIGGGVPIIGAMGKLQFEVFQRRLKDEYGADTSIHILPYGISRWVKKE
HHCCHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
DRSKIPSNANLVEDLFGNMALLFDTEWDMNYFHKNNEGIELLDNPPLEE
HHHCCCCCCHHHHHHHCCEEEEEECCCCCHHEECCCCCCEECCCCCCCC
>Mature Secondary Structure 
SPEIIEKEVNRRKTFAIIAHPDAGKTTLTEKLLLYGGAIQLAGAVKAKKEGKSATSDWM
CHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
AMEKERGISITSAALQFEYKNHILNLLDTPGHEDFSEDTYRTLMAADTAVMVLDAGKGVE
HHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCC
PQTIKLFRVCRDRGIPIITFINKMDRPTKDLYALLDEIEKVLGIKAVPDVWPLGTGFDFK
HHHHHHHHHHHHCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GVYDLRDQQLYLFDRTPGGKQKAGFRMAGPNDTSLDDQFDEEIVKAFREQIDLVENGIGQ
CEEECCCCEEEEEECCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
VDKNSFLLGKETPVYFGSAVNNFGIELFLNKFLELAPGPDHIPLRDGNYLDPVNSPFSAF
CCCCCEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHE
VFKVQANMNKAHRDRIAFLRICSGVFERGLNVNHNRLDKPVKLSSSFAFFGQDRNTVDLA
EEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEECCCEEEECCCCCEEEEE
YPGDIIGLVNPGTYKIGDVLSTGNTPPLRPLPSFAPELFATISCKDTLQLKSFKKGLDQL
CCCCEEEEECCCCEEHHHHHHCCCCCCCCCCCCHHHHHHEEECCCHHHHHHHHHHHHHHH
AEEGILHLFTSRTIGGGVPIIGAMGKLQFEVFQRRLKDEYGADTSIHILPYGISRWVKKE
HHCCHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
DRSKIPSNANLVEDLFGNMALLFDTEWDMNYFHKNNEGIELLDNPPLEE
HHHCCCCCCHHHHHHHCCEEEEEECCCCCHHEECCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA