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The map label for this gene is ddl [H]

Identifier: 183221182

GI number: 183221182

Start: 1873417

End: 1874406

Strand: Reverse

Name: ddl [H]

Synonym: LEPBI_I1796

Alternate gene names: 183221182

Gene position: 1874406-1873417 (Counterclockwise)

Preceding gene: 183221183

Following gene: 183221181

Centisome position: 52.07

GC content: 37.98

Gene sequence:

>990_bases
ATGAAAACTGTTCTCATCGCTTGTGATATCTACGACAAACAACATCCAAAACTTTCCCAAGAATGGGAATCGGAAGAAAC
CATCCTTCACATGGAAAAAACGATTCAATCATTAGGTTATGATGTTGTGGTTTTAAACCATCCAACAGAAATCACCTCCG
CACTCGCTGGGATTCCTAAATCCAATCGAAAGAATTGGATCGTATGGAATTTCATTGAAGGGTATTTATCACCATCAAGA
GAAGCGTATATTCCTGCGTTATGCGAATATTTAGGGATTCCACATACAGGAAGTCATGCGGCCGTCCAAACATTGACATT
AGACAAATACAAAACCAAATTGTATTTAGCTTCGTTTGGAATTCCAACACTAGTCTCACAATTGATTGATTCGGAATCGG
AATTACCAAGGATTTCGTATCCTCTTTTTGTCAAACCCAATGGAGAAGGTTCCAGTTTAGGGATTACGGATGCGAATGTG
ATCCATTCGAAAGACGATTGGGATACACAAGTTCCAAAACTATTAGAGACATTTTCACCTTTACTTGTTGAGCCTTACCT
TTCGGGAGAAGAAGTGACTGTTGGTATTATGGGGAACCAAGGAAACTACCAAGTATTACCGATTGCCTATGTCAAAACTC
CTAGCGGAGTTTATCATGAAGGGATCAAATCAAAATCTGAATTTTTAGAATCCCTAGATTTTGACTTACCAAATCATTTA
TGTAGTCAGATAGAATCGATCTCCTTACAAATTGCAGAACGATTAGGCTCTTCCGGGTATCTGCGAATCGATTTTAAATT
AGAAAATGAGAAACCCTTTTTTTTAGAAGCCAATGCGACTCCTGGTTTCTCTCATATTTACTCCACCTTGCCTTTGTTAT
GGGAAAGATCAGGAAGGTCTTATTCTGAACTTCTTCAATATTCATTGGAACTAGGATTTGAAGAATTTTTTACACAGAAA
CGGTTCCAATATGGAAAGGACCAATTATGA

Upstream 100 bases:

>100_bases
TGACAAAAAAGATCCATTTCTCTTTTGAACCAATTGGTTCAAACAAGGAATCTTATTATCCAAAAATCTCAGATGAAGAA
TGGGAGAAATGGAAACCAAG

Downstream 100 bases:

>100_bases
ACAACTTCATTCAAACTTTAAAAAATGACGTAGAAACCCATCCCGTTTTGCAATCAAAATGGTTAAAAGAAAGAAAACTA
AAGTTTAGTTTTGACGATTT

Product: putative D-alanine--d-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKTVLIACDIYDKQHPKLSQEWESEETILHMEKTIQSLGYDVVVLNHPTEITSALAGIPKSNRKNWIVWNFIEGYLSPSR
EAYIPALCEYLGIPHTGSHAAVQTLTLDKYKTKLYLASFGIPTLVSQLIDSESELPRISYPLFVKPNGEGSSLGITDANV
IHSKDDWDTQVPKLLETFSPLLVEPYLSGEEVTVGIMGNQGNYQVLPIAYVKTPSGVYHEGIKSKSEFLESLDFDLPNHL
CSQIESISLQIAERLGSSGYLRIDFKLENEKPFFLEANATPGFSHIYSTLPLLWERSGRSYSELLQYSLELGFEEFFTQK
RFQYGKDQL

Sequences:

>Translated_329_residues
MKTVLIACDIYDKQHPKLSQEWESEETILHMEKTIQSLGYDVVVLNHPTEITSALAGIPKSNRKNWIVWNFIEGYLSPSR
EAYIPALCEYLGIPHTGSHAAVQTLTLDKYKTKLYLASFGIPTLVSQLIDSESELPRISYPLFVKPNGEGSSLGITDANV
IHSKDDWDTQVPKLLETFSPLLVEPYLSGEEVTVGIMGNQGNYQVLPIAYVKTPSGVYHEGIKSKSEFLESLDFDLPNHL
CSQIESISLQIAERLGSSGYLRIDFKLENEKPFFLEANATPGFSHIYSTLPLLWERSGRSYSELLQYSLELGFEEFFTQK
RFQYGKDQL
>Mature_329_residues
MKTVLIACDIYDKQHPKLSQEWESEETILHMEKTIQSLGYDVVVLNHPTEITSALAGIPKSNRKNWIVWNFIEGYLSPSR
EAYIPALCEYLGIPHTGSHAAVQTLTLDKYKTKLYLASFGIPTLVSQLIDSESELPRISYPLFVKPNGEGSSLGITDANV
IHSKDDWDTQVPKLLETFSPLLVEPYLSGEEVTVGIMGNQGNYQVLPIAYVKTPSGVYHEGIKSKSEFLESLDFDLPNHL
CSQIESISLQIAERLGSSGYLRIDFKLENEKPFFLEANATPGFSHIYSTLPLLWERSGRSYSELLQYSLELGFEEFFTQK
RFQYGKDQL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=262, Percent_Identity=26.7175572519084, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786579, Length=262, Percent_Identity=25.9541984732824, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 37185; Mature: 37185

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVLIACDIYDKQHPKLSQEWESEETILHMEKTIQSLGYDVVVLNHPTEITSALAGIPK
CCEEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCC
SNRKNWIVWNFIEGYLSPSREAYIPALCEYLGIPHTGSHAAVQTLTLDKYKTKLYLASFG
CCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHEEEEEECCC
IPTLVSQLIDSESELPRISYPLFVKPNGEGSSLGITDANVIHSKDDWDTQVPKLLETFSP
HHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEECCCCCCEEECCCCCCHHHHHHHHHHCH
LLVEPYLSGEEVTVGIMGNQGNYQVLPIAYVKTPSGVYHEGIKSKSEFLESLDFDLPNHL
HEECCCCCCCEEEEEEEECCCCEEEEEEEEEECCCCHHHHHCCCHHHHHHHHCCCCHHHH
CSQIESISLQIAERLGSSGYLRIDFKLENEKPFFLEANATPGFSHIYSTLPLLWERSGRS
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
YSELLQYSLELGFEEFFTQKRFQYGKDQL
HHHHHHHHHHCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKTVLIACDIYDKQHPKLSQEWESEETILHMEKTIQSLGYDVVVLNHPTEITSALAGIPK
CCEEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCC
SNRKNWIVWNFIEGYLSPSREAYIPALCEYLGIPHTGSHAAVQTLTLDKYKTKLYLASFG
CCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHEEEEEECCC
IPTLVSQLIDSESELPRISYPLFVKPNGEGSSLGITDANVIHSKDDWDTQVPKLLETFSP
HHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEECCCCCCEEECCCCCCHHHHHHHHHHCH
LLVEPYLSGEEVTVGIMGNQGNYQVLPIAYVKTPSGVYHEGIKSKSEFLESLDFDLPNHL
HEECCCCCCCEEEEEEEECCCCEEEEEEEEEECCCCHHHHHCCCHHHHHHHHCCCCHHHH
CSQIESISLQIAERLGSSGYLRIDFKLENEKPFFLEANATPGFSHIYSTLPLLWERSGRS
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
YSELLQYSLELGFEEFFTQKRFQYGKDQL
HHHHHHHHHHCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA