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The map label for this gene is gatA

Identifier: 183221116

GI number: 183221116

Start: 1802975

End: 1804441

Strand: Reverse

Name: gatA

Synonym: LEPBI_I1730

Alternate gene names: 183221116

Gene position: 1804441-1802975 (Counterclockwise)

Preceding gene: 183221117

Following gene: 183221115

Centisome position: 50.13

GC content: 37.15

Gene sequence:

>1467_bases
ATGAAAGATTTAATATTTTTAACTTATTCTGAAATCAAAAAAAGATTAAATGAAGGATCTTTATCTTCGGAAGAATTGGT
ATCTGCCTACTTAGAAAGGATCAATGCAGTTGATTCGAAACTAAAAGCATTTTTGGAATTGAACCAAGAAAAAATCCTCA
AACAAGCTAAAGAAAGTGATGATAGACGTAAAAATGGAAAACTTCTCTCTGAATTTGATGGAATTCCCATTGGCATCAAA
GATAATATCTGTATCACAGGTGAAGTCACCTCATGTTCATCTAAAATTTTAGAGAATTTTGTATCACCTTATGATGCAAC
AGTCATTTCCAAGTTAAAGGAAAAAGGATTTGTATTATTCCCTCGTCTCAATATGGACGAATTTGCAATGGGCTCCTCTA
CAGAGAACAGTGCTTTCCAAACATCCAAAAATCCATTTGATTCAGATCGAATTCCTGGAGGTTCTAGCGGTGGTTCTGCG
GCAGCAGTTGCCGGATCCATGTTACCAATCTCCCTCGGCTCGGATACTGGTGGATCCATCCGCCAACCTGCATCCCTCTG
TGGAATTTGGGGATTAAAGCCAACCTATGGTAGAGTTTCACGGTATGGCCTTGTTGCCTATGCATCAAGTCTTGATCAAA
TTGGTCCATTTTCAAATGATATGGATGGAATCAGTGATCTACTTGAAATCCTATCAGGGATCGATTCAAAAGACCAAACA
ACTTCCAAAGTGAATCAATTCGAATCAAAATCTGTGAATGAAATTGACTGGAAATGCAAAAAAATTGGAATCATGAAAAC
TGAGGATTTTAATTTTTCACCAGATGTGAACGCTCGTTATCTGGAAATTTTAAAAACCTTAGAAGGCAAAGGTGCCGAGC
TCGTTCCTTTAGATTTTTCATTATTAAAGTATGCGATTCCAGTGTATTATCTTATCGCTACTGCCGAATGTTCCTCTAAT
CTAAGCCGTTTTGATGGCATCAGATATGGACTTAGAAAAGAAACTTCTGGAAAACTAGATGATTTGTATTCTGAATCCAG
AAGTGCTGGGTTTGGAAAAGAAGTGAAACGAAGAATTTTACTCGGTACATTTTCACTTAGCTCTGGTTATTATGATGCTT
ATTATGGAAAGGCACAGAAGGCAAGAGTTCTCATCAGAAAACAGTATGCAGAATTTTTTAAAACAGTTGATGTCATTTTG
CAACCTACCTCTCCTACCACAGCCTTTAAAGTGGGAGAAAAAACGAAAGATCCAATTCAAATGTACCAAGCTGATATATT
AACCACTTCTGTGAATTTGGCTGGAGTTCCCGCGATCAGTTGTCCTGCGGGATTGGATCAAAATGGTCTACCGATCGGAG
TCCAATTGACAACATCTCATTTTGATGAAAACAAATTACTCGGGTTTGCAAAATCATTATCAAAGTTAGACATTTGTCAG
ATTTCCTTACCAAAAGAGATCAAATAA

Upstream 100 bases:

>100_bases
GAAAGGATTTAAGTGAAAACTCATTAAAACGAGAAGACTTATCAAAAATTTCTCCTTCGTATGAAAATGGTTATGTGGTG
GTTCCTAAGGTAATTGAAAC

Downstream 100 bases:

>100_bases
AATGGATGAACTTACCAAAAGAGTCATTCCCTGTTTGGACATCAAAGGGGGAAGGGTCGTTAAAGGAGTTCAATTTGTCA
ATTTAATCGATGCAGGAGAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKDLIFLTYSEIKKRLNEGSLSSEELVSAYLERINAVDSKLKAFLELNQEKILKQAKESDDRRKNGKLLSEFDGIPIGIK
DNICITGEVTSCSSKILENFVSPYDATVISKLKEKGFVLFPRLNMDEFAMGSSTENSAFQTSKNPFDSDRIPGGSSGGSA
AAVAGSMLPISLGSDTGGSIRQPASLCGIWGLKPTYGRVSRYGLVAYASSLDQIGPFSNDMDGISDLLEILSGIDSKDQT
TSKVNQFESKSVNEIDWKCKKIGIMKTEDFNFSPDVNARYLEILKTLEGKGAELVPLDFSLLKYAIPVYYLIATAECSSN
LSRFDGIRYGLRKETSGKLDDLYSESRSAGFGKEVKRRILLGTFSLSSGYYDAYYGKAQKARVLIRKQYAEFFKTVDVIL
QPTSPTTAFKVGEKTKDPIQMYQADILTTSVNLAGVPAISCPAGLDQNGLPIGVQLTTSHFDENKLLGFAKSLSKLDICQ
ISLPKEIK

Sequences:

>Translated_488_residues
MKDLIFLTYSEIKKRLNEGSLSSEELVSAYLERINAVDSKLKAFLELNQEKILKQAKESDDRRKNGKLLSEFDGIPIGIK
DNICITGEVTSCSSKILENFVSPYDATVISKLKEKGFVLFPRLNMDEFAMGSSTENSAFQTSKNPFDSDRIPGGSSGGSA
AAVAGSMLPISLGSDTGGSIRQPASLCGIWGLKPTYGRVSRYGLVAYASSLDQIGPFSNDMDGISDLLEILSGIDSKDQT
TSKVNQFESKSVNEIDWKCKKIGIMKTEDFNFSPDVNARYLEILKTLEGKGAELVPLDFSLLKYAIPVYYLIATAECSSN
LSRFDGIRYGLRKETSGKLDDLYSESRSAGFGKEVKRRILLGTFSLSSGYYDAYYGKAQKARVLIRKQYAEFFKTVDVIL
QPTSPTTAFKVGEKTKDPIQMYQADILTTSVNLAGVPAISCPAGLDQNGLPIGVQLTTSHFDENKLLGFAKSLSKLDICQ
ISLPKEIK
>Mature_488_residues
MKDLIFLTYSEIKKRLNEGSLSSEELVSAYLERINAVDSKLKAFLELNQEKILKQAKESDDRRKNGKLLSEFDGIPIGIK
DNICITGEVTSCSSKILENFVSPYDATVISKLKEKGFVLFPRLNMDEFAMGSSTENSAFQTSKNPFDSDRIPGGSSGGSA
AAVAGSMLPISLGSDTGGSIRQPASLCGIWGLKPTYGRVSRYGLVAYASSLDQIGPFSNDMDGISDLLEILSGIDSKDQT
TSKVNQFESKSVNEIDWKCKKIGIMKTEDFNFSPDVNARYLEILKTLEGKGAELVPLDFSLLKYAIPVYYLIATAECSSN
LSRFDGIRYGLRKETSGKLDDLYSESRSAGFGKEVKRRILLGTFSLSSGYYDAYYGKAQKARVLIRKQYAEFFKTVDVIL
QPTSPTTAFKVGEKTKDPIQMYQADILTTSVNLAGVPAISCPAGLDQNGLPIGVQLTTSHFDENKLLGFAKSLSKLDICQ
ISLPKEIK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=37.5249500998004, Blast_Score=283, Evalue=4e-76,
Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=24.5472837022133, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI166795287, Length=240, Percent_Identity=30.4166666666667, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=447, Percent_Identity=34.0044742729307, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17537465, Length=500, Percent_Identity=26.2, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17556264, Length=201, Percent_Identity=29.8507462686567, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=343, Percent_Identity=26.2390670553936, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=330, Percent_Identity=27.8787878787879, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=302, Percent_Identity=24.8344370860927, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=191, Percent_Identity=30.8900523560209, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=191, Percent_Identity=30.8900523560209, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=466, Percent_Identity=34.7639484978541, Blast_Score=233, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6319685, Length=434, Percent_Identity=28.3410138248848, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=529, Percent_Identity=22.8733459357278, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648113, Length=489, Percent_Identity=37.0143149284254, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24644968, Length=493, Percent_Identity=25.9634888438134, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24652985, Length=506, Percent_Identity=24.901185770751, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19922090, Length=506, Percent_Identity=24.901185770751, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24652981, Length=506, Percent_Identity=24.901185770751, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24652983, Length=506, Percent_Identity=24.901185770751, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI45550774, Length=200, Percent_Identity=33.5, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648435, Length=208, Percent_Identity=32.6923076923077, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648437, Length=208, Percent_Identity=32.6923076923077, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078093, Length=513, Percent_Identity=25.3411306042885, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21356731, Length=232, Percent_Identity=31.0344827586207, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648441, Length=135, Percent_Identity=37.037037037037, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648439, Length=135, Percent_Identity=37.037037037037, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_LEPBA (B0S8V3)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962762.1
- ProteinModelPortal:   B0S8V3
- SMR:   B0S8V3
- GeneID:   6388959
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1677
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   LBIF355278:LBF_1677-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53512; Mature: 53512

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 80-80 ACT_SITE 155-155 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLIFLTYSEIKKRLNEGSLSSEELVSAYLERINAVDSKLKAFLELNQEKILKQAKESD
CCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DRRKNGKLLSEFDGIPIGIKDNICITGEVTSCSSKILENFVSPYDATVISKLKEKGFVLF
HHHHCCCHHHHCCCCEECCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEE
PRLNMDEFAMGSSTENSAFQTSKNPFDSDRIPGGSSGGSAAAVAGSMLPISLGSDTGGSI
ECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCC
RQPASLCGIWGLKPTYGRVSRYGLVAYASSLDQIGPFSNDMDGISDLLEILSGIDSKDQT
CCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHH
TSKVNQFESKSVNEIDWKCKKIGIMKTEDFNFSPDVNARYLEILKTLEGKGAELVPLDFS
HHHHHHHHCCCCCHHCCHHHHCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCHH
LLKYAIPVYYLIATAECSSNLSRFDGIRYGLRKETSGKLDDLYSESRSAGFGKEVKRRIL
HHHHHHHHHHHEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
LGTFSLSSGYYDAYYGKAQKARVLIRKQYAEFFKTVDVILQPTSPTTAFKVGEKTKDPIQ
EEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCCCHHH
MYQADILTTSVNLAGVPAISCPAGLDQNGLPIGVQLTTSHFDENKLLGFAKSLSKLDICQ
HHHHHHHHEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEE
ISLPKEIK
ECCCCCCC
>Mature Secondary Structure
MKDLIFLTYSEIKKRLNEGSLSSEELVSAYLERINAVDSKLKAFLELNQEKILKQAKESD
CCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DRRKNGKLLSEFDGIPIGIKDNICITGEVTSCSSKILENFVSPYDATVISKLKEKGFVLF
HHHHCCCHHHHCCCCEECCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEE
PRLNMDEFAMGSSTENSAFQTSKNPFDSDRIPGGSSGGSAAAVAGSMLPISLGSDTGGSI
ECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCC
RQPASLCGIWGLKPTYGRVSRYGLVAYASSLDQIGPFSNDMDGISDLLEILSGIDSKDQT
CCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHH
TSKVNQFESKSVNEIDWKCKKIGIMKTEDFNFSPDVNARYLEILKTLEGKGAELVPLDFS
HHHHHHHHCCCCCHHCCHHHHCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCHH
LLKYAIPVYYLIATAECSSNLSRFDGIRYGLRKETSGKLDDLYSESRSAGFGKEVKRRIL
HHHHHHHHHHHEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
LGTFSLSSGYYDAYYGKAQKARVLIRKQYAEFFKTVDVILQPTSPTTAFKVGEKTKDPIQ
EEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCCCHHH
MYQADILTTSVNLAGVPAISCPAGLDQNGLPIGVQLTTSHFDENKLLGFAKSLSKLDICQ
HHHHHHHHEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEE
ISLPKEIK
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA