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The map label for this gene is hisF

Identifier: 183221115

GI number: 183221115

Start: 1802206

End: 1802973

Strand: Reverse

Name: hisF

Synonym: LEPBI_I1729

Alternate gene names: 183221115

Gene position: 1802973-1802206 (Counterclockwise)

Preceding gene: 183221116

Following gene: 183221114

Centisome position: 50.09

GC content: 41.41

Gene sequence:

>768_bases
ATGGATGAACTTACCAAAAGAGTCATTCCCTGTTTGGACATCAAAGGGGGAAGGGTCGTTAAAGGAGTTCAATTTGTCAA
TTTAATCGATGCAGGAGACCCTGTTTCTTGTGCTGTCGCTTACGAAGAAAACAAAGCGGATGAACTTTGTTTTTTAGATA
TCACTGCTTCTTCCGACAAACGAGACATTCTACTACATCTCGTCGAAGAAGTGGCGAACAAACTTTTTATCCCATTCACT
GTCGGTGGTGGCATTCGAACAATCGAGGATGTAAAGGCAGTGCTCAATAAAGGAGCTGATAAAGTATCCATCAATACGAG
TGCCTTCCAAAATCCGAAACTCTTAAAAGATGCAAGTGAAATTTACGGATCACAATGCATAGTCTGTGCCATTGACGTTA
AGTTTCATCCAGAACGCAAACGGTACGAAGTGTATCTGAATGGTGGTAGAGCAGAAACTGGTAGAGAAGCATTAGATTGG
GGAAAAGAGGCGCATGAAATGGGAGCCGGTGAAATATTACTGACATCCATGGACAAAGATGGAACCAAAGACGGATTCGA
TATCAATCTTATGAAATCGTTTACTTCCAATCTAACGATCCCAATCATTGCGTCTGGTGGTGCTGGTAATCCAGAACATA
TGGCAGAAGTGATCTTAAGAGGTGGAGCTGATGCTGTACTTGCAGCCTCTATCTTTCACTTTGGAGAATTTTCCATCCAA
GAAACTAAACAAACCATGAAAGAGATGGGAATCAAAGTTAGATTATGA

Upstream 100 bases:

>100_bases
ACAACATCTCATTTTGATGAAAACAAATTACTCGGGTTTGCAAAATCATTATCAAAGTTAGACATTTGTCAGATTTCCTT
ACCAAAAGAGATCAAATAAA

Downstream 100 bases:

>100_bases
ATTCATTTCATATCTTAGATTATCTTTTCTTTTTCTTTCCATTTATAATCATTCTCCTAATCCTCGTTCGGTTTCGTTCC
AAATCCAACTCCACCAAAGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MDELTKRVIPCLDIKGGRVVKGVQFVNLIDAGDPVSCAVAYEENKADELCFLDITASSDKRDILLHLVEEVANKLFIPFT
VGGGIRTIEDVKAVLNKGADKVSINTSAFQNPKLLKDASEIYGSQCIVCAIDVKFHPERKRYEVYLNGGRAETGREALDW
GKEAHEMGAGEILLTSMDKDGTKDGFDINLMKSFTSNLTIPIIASGGAGNPEHMAEVILRGGADAVLAASIFHFGEFSIQ
ETKQTMKEMGIKVRL

Sequences:

>Translated_255_residues
MDELTKRVIPCLDIKGGRVVKGVQFVNLIDAGDPVSCAVAYEENKADELCFLDITASSDKRDILLHLVEEVANKLFIPFT
VGGGIRTIEDVKAVLNKGADKVSINTSAFQNPKLLKDASEIYGSQCIVCAIDVKFHPERKRYEVYLNGGRAETGREALDW
GKEAHEMGAGEILLTSMDKDGTKDGFDINLMKSFTSNLTIPIIASGGAGNPEHMAEVILRGGADAVLAASIFHFGEFSIQ
ETKQTMKEMGIKVRL
>Mature_255_residues
MDELTKRVIPCLDIKGGRVVKGVQFVNLIDAGDPVSCAVAYEENKADELCFLDITASSDKRDILLHLVEEVANKLFIPFT
VGGGIRTIEDVKAVLNKGADKVSINTSAFQNPKLLKDASEIYGSQCIVCAIDVKFHPERKRYEVYLNGGRAETGREALDW
GKEAHEMGAGEILLTSMDKDGTKDGFDINLMKSFTSNLTIPIIASGGAGNPEHMAEVILRGGADAVLAASIFHFGEFSIQ
ETKQTMKEMGIKVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=43.359375, Blast_Score=206, Evalue=8e-55,
Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=27.0833333333333, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=33.7579617834395, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_LEPBA (B0S8V2)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962761.1
- ProteinModelPortal:   B0S8V2
- SMR:   B0S8V2
- GeneID:   6387231
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1676
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   LBIF355278:LBF_1676-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27751; Mature: 27751

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: ACT_SITE 13-13 ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDELTKRVIPCLDIKGGRVVKGVQFVNLIDAGDPVSCAVAYEENKADELCFLDITASSDK
CCCHHHHHCCEEECCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCC
RDILLHLVEEVANKLFIPFTVGGGIRTIEDVKAVLNKGADKVSINTSAFQNPKLLKDASE
HHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
IYGSQCIVCAIDVKFHPERKRYEVYLNGGRAETGREALDWGKEAHEMGAGEILLTSMDKD
HCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHCCHHHHHCCCCEEEEEECCCC
GTKDGFDINLMKSFTSNLTIPIIASGGAGNPEHMAEVILRGGADAVLAASIFHFGEFSIQ
CCCCCCEEEHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHH
ETKQTMKEMGIKVRL
HHHHHHHHCCCEECC
>Mature Secondary Structure
MDELTKRVIPCLDIKGGRVVKGVQFVNLIDAGDPVSCAVAYEENKADELCFLDITASSDK
CCCHHHHHCCEEECCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCC
RDILLHLVEEVANKLFIPFTVGGGIRTIEDVKAVLNKGADKVSINTSAFQNPKLLKDASE
HHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
IYGSQCIVCAIDVKFHPERKRYEVYLNGGRAETGREALDWGKEAHEMGAGEILLTSMDKD
HCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHCCHHHHHCCCCEEEEEECCCC
GTKDGFDINLMKSFTSNLTIPIIASGGAGNPEHMAEVILRGGADAVLAASIFHFGEFSIQ
CCCCCCEEEHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHH
ETKQTMKEMGIKVRL
HHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA