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The map label for this gene is cheB4 [H]

Identifier: 183220966

GI number: 183220966

Start: 1653147

End: 1654199

Strand: Reverse

Name: cheB4 [H]

Synonym: LEPBI_I1579

Alternate gene names: 183220966

Gene position: 1654199-1653147 (Counterclockwise)

Preceding gene: 183220967

Following gene: 183220960

Centisome position: 45.95

GC content: 42.55

Gene sequence:

>1053_bases
ATGAATCGAATTAAGGTTTTTGTGATCGATGACTCTGCTGTGGTCCGGCAAGTGCTTACTGAAATTTTTAAACAAGATTC
ACATTTTTTATTCTTAGGAAGTGCATCCGATCCCATCTTTGCCATGGAGAAAATGAACAATGATTGGCCCGATGTGATCG
TACTTGATATCGAAATGCCTAGAATGGATGGACTTTCTTTCTTAAAAAAAATTATGACAGAAAGACCAACACCAGTTGTG
ATTTGTTCTACCCTCACTACAGAAGGTTCAGAAACTGCTATACTTGCCATGAACCTTGGTGCCTGTGACATCATCACCAA
ACCAAAAATTGGATTAAAAGATTTTTTAAATGAAAGTACCATGGCACTGACGGATGCGGTGATCGCTGCTGCATCAGCTT
CGATGAAACACCTTCCCAATCCCACCAAAACAACAGAATTCAAAATACAACTGGAGAAAAAACAAGAACTTTCCTCCTTA
CAAGCCACAGAAAAAATCATCGCGATTGGAACATCCACTGGTGGTACCATAGCCCTCGAACAGGTGTTAACCAAATTACC
GAGGGATAAAACCCCAGGAATCGTGATTGTCCAACATATGCCAGAAAAATTCACAGAAGCCTTTGCCAAACGATTGGATT
CCATTTGTGAAATTCGTGTGAAAGAAGCAAAAGATGGAGACCGAGTGGTGAGAGGACTTGCGCTCATCGCTCCCGGCAAC
AAACATATGACACTAAAACGATCCGGTGCGCAGTACTACGTAGAAGTGATGGATGGGCCACTTGTGAATCGTCACAAACC
GTCGGTGGATGTATTATTTCGTTCCGTTGCAAAACATGCCGGTAAAAATGCGAGAGGGATCATCATGACGGGAATGGGAG
ATGATGGAGCTTTGGGTTTACTGGAAATGAAAGAAGCTGGGGCAGAAACCATAGCCCAAAACGAAGAAACTTCAGTTGTC
TTTGGAATGCCGAAAGAAGCGATCAAAAGAGGATGTGTTGACCACATCCTCCCGTTAGGTGAAATTTATAAAACGATCGT
AGGCTTCGGTTAA

Upstream 100 bases:

>100_bases
TCAAATCAAAATTATTTCTGAAGACCTTGGTGGAAACCAATCGAGAAAAATATATTTTTCTGTTTGGGACGGCGAAGTGT
GGGTCGAAAAAAAATAAGCC

Downstream 100 bases:

>100_bases
TTAACCGAGCCCCAATTCTTTTGTTACTTTCACTTTTCTTGCGCGAATGTCCTCAGGAGATTCTTCCTTCAAAATTTCAT
CTGGTTTGATTTTAAAGATG

Product: protein-glutamate methylesterase CheB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNRIKVFVIDDSAVVRQVLTEIFKQDSHFLFLGSASDPIFAMEKMNNDWPDVIVLDIEMPRMDGLSFLKKIMTERPTPVV
ICSTLTTEGSETAILAMNLGACDIITKPKIGLKDFLNESTMALTDAVIAAASASMKHLPNPTKTTEFKIQLEKKQELSSL
QATEKIIAIGTSTGGTIALEQVLTKLPRDKTPGIVIVQHMPEKFTEAFAKRLDSICEIRVKEAKDGDRVVRGLALIAPGN
KHMTLKRSGAQYYVEVMDGPLVNRHKPSVDVLFRSVAKHAGKNARGIIMTGMGDDGALGLLEMKEAGAETIAQNEETSVV
FGMPKEAIKRGCVDHILPLGEIYKTIVGFG

Sequences:

>Translated_350_residues
MNRIKVFVIDDSAVVRQVLTEIFKQDSHFLFLGSASDPIFAMEKMNNDWPDVIVLDIEMPRMDGLSFLKKIMTERPTPVV
ICSTLTTEGSETAILAMNLGACDIITKPKIGLKDFLNESTMALTDAVIAAASASMKHLPNPTKTTEFKIQLEKKQELSSL
QATEKIIAIGTSTGGTIALEQVLTKLPRDKTPGIVIVQHMPEKFTEAFAKRLDSICEIRVKEAKDGDRVVRGLALIAPGN
KHMTLKRSGAQYYVEVMDGPLVNRHKPSVDVLFRSVAKHAGKNARGIIMTGMGDDGALGLLEMKEAGAETIAQNEETSVV
FGMPKEAIKRGCVDHILPLGEIYKTIVGFG
>Mature_350_residues
MNRIKVFVIDDSAVVRQVLTEIFKQDSHFLFLGSASDPIFAMEKMNNDWPDVIVLDIEMPRMDGLSFLKKIMTERPTPVV
ICSTLTTEGSETAILAMNLGACDIITKPKIGLKDFLNESTMALTDAVIAAASASMKHLPNPTKTTEFKIQLEKKQELSSL
QATEKIIAIGTSTGGTIALEQVLTKLPRDKTPGIVIVQHMPEKFTEAFAKRLDSICEIRVKEAKDGDRVVRGLALIAPGN
KHMTLKRSGAQYYVEVMDGPLVNRHKPSVDVLFRSVAKHAGKNARGIIMTGMGDDGALGLLEMKEAGAETIAQNEETSVV
FGMPKEAIKRGCVDHILPLGEIYKTIVGFG

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR [H]

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=348, Percent_Identity=49.7126436781609, Blast_Score=339, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg [H]

EC number: =3.1.1.61 [H]

Molecular weight: Translated: 38238; Mature: 38238

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIKVFVIDDSAVVRQVLTEIFKQDSHFLFLGSASDPIFAMEKMNNDWPDVIVLDIEMP
CCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEHHHCCCCCCCEEEEEECCC
RMDGLSFLKKIMTERPTPVVICSTLTTEGSETAILAMNLGACDIITKPKIGLKDFLNEST
CCCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCEEEECCCCCCHHHHHCCHH
MALTDAVIAAASASMKHLPNPTKTTEFKIQLEKKQELSSLQATEKIIAIGTSTGGTIALE
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHCEEEEEECCCCCHHHHH
QVLTKLPRDKTPGIVIVQHMPEKFTEAFAKRLDSICEIRVKEAKDGDRVVRGLALIAPGN
HHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEEEECCC
KHMTLKRSGAQYYVEVMDGPLVNRHKPSVDVLFRSVAKHAGKNARGIIMTGMGDDGALGL
CEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEH
LEMKEAGAETIAQNEETSVVFGMPKEAIKRGCVDHILPLGEIYKTIVGFG
HHHHHCCHHHHHCCCCCEEEEECCHHHHHCCHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MNRIKVFVIDDSAVVRQVLTEIFKQDSHFLFLGSASDPIFAMEKMNNDWPDVIVLDIEMP
CCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEHHHCCCCCCCEEEEEECCC
RMDGLSFLKKIMTERPTPVVICSTLTTEGSETAILAMNLGACDIITKPKIGLKDFLNEST
CCCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCEEEECCCCCCHHHHHCCHH
MALTDAVIAAASASMKHLPNPTKTTEFKIQLEKKQELSSLQATEKIIAIGTSTGGTIALE
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHCEEEEEECCCCCHHHHH
QVLTKLPRDKTPGIVIVQHMPEKFTEAFAKRLDSICEIRVKEAKDGDRVVRGLALIAPGN
HHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEEEECCC
KHMTLKRSGAQYYVEVMDGPLVNRHKPSVDVLFRSVAKHAGKNARGIIMTGMGDDGALGL
CEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEH
LEMKEAGAETIAQNEETSVVFGMPKEAIKRGCVDHILPLGEIYKTIVGFG
HHHHHCCHHHHHCCCCCEEEEECCHHHHHCCHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA