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The map label for this gene is def

Identifier: 183220960

GI number: 183220960

Start: 1644490

End: 1645029

Strand: Reverse

Name: def

Synonym: LEPBI_I1573

Alternate gene names: 183220960

Gene position: 1645029-1644490 (Counterclockwise)

Preceding gene: 183220966

Following gene: 183220959

Centisome position: 45.7

GC content: 42.04

Gene sequence:

>540_bases
ATGGCTGTACGTAAAATTCTCAAAATTGGTAATCCGATCCTTAGGCAAACGAGCGAAGATGTCTCCGAATCCGAAATCCA
AACCAAAGATTTCAAAAAATTGATCCGCGACATGTTTGAAACCATGCGCCACGCAGATGGAGTGGGACTTGCCGCACCAC
AAATCGGTGTTTTAAAGAAACTAGTCGTTGTTGGCCAAGAAGATGACAACGAACGTTACCCAGGGACACCCGAAGTTCCA
AACCAAATCATCTTAAATCCAGAAATCACTCCTCTAAGCCCGCCAAGAGATGGGTTTTGGGAAGGATGTCTCTCTGTACC
TGGAATGCGAGGCTATGTGGAAAGACCAAACAAAATCCGCATGAAATGGCGAGATGAAAATTATGTAGAACATGACGAGA
TCATTGAAGGGTATAGAGCCATCGTCTTACAACATGAATGTGATCATCTTTTTGGAGTTTTGTATGTGGATCGATTGAAA
AGTACAAAGTTATTTGGTTATAACGAAGACATCGATACCGCAGGAAAGTTGTTAGATTAA

Upstream 100 bases:

>100_bases
ATTTCAGCTTTTCAAGGCTTCCCTGTCAAATTCTTTTTCATTTGGTTAGGAGTTCACTTGATCATTCAGTGACCTTTTTC
AATCTATCCCATATTAGATT

Downstream 100 bases:

>100_bases
TTTTTTGTTTTGTTTAAAGTTGCAATAGTCCCCCACGAGGAGATTCCATGGGTTCATCCATAGTTCAATATTTTCTTTCT
AAAAGTTTATTCGTAAACCT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MAVRKILKIGNPILRQTSEDVSESEIQTKDFKKLIRDMFETMRHADGVGLAAPQIGVLKKLVVVGQEDDNERYPGTPEVP
NQIILNPEITPLSPPRDGFWEGCLSVPGMRGYVERPNKIRMKWRDENYVEHDEIIEGYRAIVLQHECDHLFGVLYVDRLK
STKLFGYNEDIDTAGKLLD

Sequences:

>Translated_179_residues
MAVRKILKIGNPILRQTSEDVSESEIQTKDFKKLIRDMFETMRHADGVGLAAPQIGVLKKLVVVGQEDDNERYPGTPEVP
NQIILNPEITPLSPPRDGFWEGCLSVPGMRGYVERPNKIRMKWRDENYVEHDEIIEGYRAIVLQHECDHLFGVLYVDRLK
STKLFGYNEDIDTAGKLLD
>Mature_178_residues
AVRKILKIGNPILRQTSEDVSESEIQTKDFKKLIRDMFETMRHADGVGLAAPQIGVLKKLVVVGQEDDNERYPGTPEVPN
QIILNPEITPLSPPRDGFWEGCLSVPGMRGYVERPNKIRMKWRDENYVEHDEIIEGYRAIVLQHECDHLFGVLYVDRLKS
TKLFGYNEDIDTAGKLLD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=164, Percent_Identity=26.8292682926829, Blast_Score=74, Evalue=9e-14,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=32.9268292682927, Blast_Score=88, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=34.3373493975904, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24645726, Length=175, Percent_Identity=30.8571428571429, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_LEPBA (B0SHH1)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962610.1
- ProteinModelPortal:   B0SHH1
- SMR:   B0SHH1
- GeneID:   6388120
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1521
- HOGENOM:   HBG665227
- OMA:   YARIRYE
- ProtClustDB:   PRK12846
- BioCyc:   LBIF355278:LBF_1521-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20480; Mature: 20349

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRKILKIGNPILRQTSEDVSESEIQTKDFKKLIRDMFETMRHADGVGLAAPQIGVLKK
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LVVVGQEDDNERYPGTPEVPNQIILNPEITPLSPPRDGFWEGCLSVPGMRGYVERPNKIR
HHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEE
MKWRDENYVEHDEIIEGYRAIVLQHECDHLFGVLYVDRLKSTKLFGYNEDIDTAGKLLD
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
AVRKILKIGNPILRQTSEDVSESEIQTKDFKKLIRDMFETMRHADGVGLAAPQIGVLKK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LVVVGQEDDNERYPGTPEVPNQIILNPEITPLSPPRDGFWEGCLSVPGMRGYVERPNKIR
HHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEE
MKWRDENYVEHDEIIEGYRAIVLQHECDHLFGVLYVDRLKSTKLFGYNEDIDTAGKLLD
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA