Definition Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)' chromosome chromosome I, complete sequence.
Accession NC_010602
Length 3,599,677

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The map label for this gene is pnp

Identifier: 183220916

GI number: 183220916

Start: 1594201

End: 1596291

Strand: Direct

Name: pnp

Synonym: LEPBI_I1529

Alternate gene names: 183220916

Gene position: 1594201-1596291 (Clockwise)

Preceding gene: 183220915

Following gene: 183220917

Centisome position: 44.29

GC content: 43.57

Gene sequence:

>2091_bases
ATGGCTACAGAGTTCACTGGTGTTTGGGGTAGAGATTCTATTACCCTAGAGACCGGCAAGTGGGCGAAACAAGCTCACGG
GTCGGTTGTATACAAAACCGGAAATTTGGTCCTGCTTGCCACAGTTTGTGCGGCAGAGGAACCAAAAGAAGGACAAGATT
TTTTCCCTCTTACATGCGAATACACTGAAAAAGCATACTCAGTCGGTCGTTTCCCAGGTGGATACTTCAAACGGGAAGCA
AAACCCGCAGAACACGAAGTATTACTTTCTCGGATCCTAGATCGTCCGATTCGCCCAATGTTCCCAGAAGGTTACTTCTC
GGAAGTACAACTTCTCGTTCAAGTTTTATCTGCAGACAAACAAGTTTCAGTCCAAGGCCATGCGATTAACGCAGCTTCGG
CGGCACTTTCAGTTTCTTCCATTCCATTTGCAGGACCCATTGCGGGAGCAAGGATTGGTCGTATCGGTGGAGAGTTCATC
CTCAATCCAACGAACGAAGAGATCACAAAGTCTGATTTGGACTTAGTGGTTGCAGGAACAAAAGATGCCATCGTCATGAT
TGAAGGGGAAGCAAGCGAAATCTCCAAAGAAGACATGATGGCAGCCCTTCGTTTTGCCCAAGAGCAACTGAAATTTGCTG
TGGCAATGCAAGAAGAATTGGCGAAGAAACATGGAACTGTTAAAAAGGAAGTTGTTTTAAAAGCTCCTGACAAAGACCTC
CATGCTAAAATTCGTGAATTCGCATTCGATCGTTTGACTCTTGCCAACAAAAATGCAGACAAAGCCAAACGGAACGACGA
CATCAAAGCCATTAACAAAGAAACGGTTGAACATTTTAAAACCTTACTGGCTCCAGAAGACAAAACAAAAGACATCAAAC
ATTTTTTACATGAATTGGAATACGAAGTTGTCCGCGAACTCGTGCTAGGCGAAGGAATTCGTTTTGATGGCCGAAAAACG
GACGAAATTCGACAAATCTCTTGTGAGATTGATGTCCTTCCTGGACCACATGGTTCGGCTGTTTTCACTAGGGGACAAAC
TCAGTCTCTTGGAGTGATGACACTCGGTACCACATCTGACAACCAACGATACGAAACACTCGAAGGTTCGAAAGAAAAGA
ACTTCATGTTACACTATAACTTCCCTGCATTTTCTGTGGGAGAAGTGAGACGTAACTCAGGCCCTGGAAGGCGTGAGATT
GGTCACGGAAATTTAGCGGAACGAGCGATTAAAAAAGTCCTTCCGACACAAACTGAGTTTCCGTATGTGATCAGGTTAGT
CTCTGAAATTTTAGAATCCAATGGATCTTCTTCCATGGCATCCGTTTGTTCGGGAACCCTTGCCCTAATGGCAGGTGGAG
TTCCGATTTCGGGAGCCGTTTCTGGGATTGCGATGGGACTTTTCAGTGATGAAAAAGGTCGTTTTGCAGTCCTTTCGGAC
ATCGCAGGGATTGAAGACCACTTCGGTGATATGGATTTCAAATTGGCAGGGACCAAAAAAGGGATCACTGCTTTCCAAAT
GGATCTCAAAGTCAATGGACTTGGTTTGGAAGTTTTACAAAAAGCCATCGAACAAGCTGAAGTGGGTCGTGACCATATCC
TTGGTGAAATGAACAAAGCCATTTCTTCCGTGAAAGGGAATTTAAGTGAAAACGCTCCGCGTATCACTCAAAAACAAATT
CCAAAAGATCGTATTGGAGAACTCATTGGCCCAGGTGGGAAAATGATCCGTGCCATCATCGAACAATCTGGTTCTGAAAT
TTCTGTGGATGATTCTGGAAAAGTAACCATTGCTTCTCCAAGTGAAGAGTCTAAAGAAAAAGCCATTGCTATGATCGATG
GAATCTTTGAAGAAATTGAAGTGGGAAAAATCTACGATGGTGTCATCAAACGAATTGCTGACTTTGGTGCCTTTGTTGAA
ATTTTACCAGGAAAAGAAGGGCTTTGCCACATCTCGAAACTGGATGTGAAACGAGTACAATCTGTTCGTGACATTGTTTC
CGAAGGAGACAAAATCAAAGTGAAAGTGATTTCCGTTGATAAAATGGGAAAAATTGATCTTTCACGAAAGGATGTCCTTT
TAGACAACTAA

Upstream 100 bases:

>100_bases
TGGTGAACCAGAGAAAGAGTTTGTTGGAATACCTAAAACGTTCCAATTTAGAAAGTTATAAAAAGCTGATTGAAAAACTC
GGCCTTAGGAAATAATTCCT

Downstream 100 bases:

>100_bases
ACAGTCCCCAAACACCTGTTTGGGGGGAGTGAAACAAATGGTTTCCGTCAGTGAATGCAAACGAACTGTTTTACCAAATG
GTTTGACAGTCCTTTTCCAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MATEFTGVWGRDSITLETGKWAKQAHGSVVYKTGNLVLLATVCAAEEPKEGQDFFPLTCEYTEKAYSVGRFPGGYFKREA
KPAEHEVLLSRILDRPIRPMFPEGYFSEVQLLVQVLSADKQVSVQGHAINAASAALSVSSIPFAGPIAGARIGRIGGEFI
LNPTNEEITKSDLDLVVAGTKDAIVMIEGEASEISKEDMMAALRFAQEQLKFAVAMQEELAKKHGTVKKEVVLKAPDKDL
HAKIREFAFDRLTLANKNADKAKRNDDIKAINKETVEHFKTLLAPEDKTKDIKHFLHELEYEVVRELVLGEGIRFDGRKT
DEIRQISCEIDVLPGPHGSAVFTRGQTQSLGVMTLGTTSDNQRYETLEGSKEKNFMLHYNFPAFSVGEVRRNSGPGRREI
GHGNLAERAIKKVLPTQTEFPYVIRLVSEILESNGSSSMASVCSGTLALMAGGVPISGAVSGIAMGLFSDEKGRFAVLSD
IAGIEDHFGDMDFKLAGTKKGITAFQMDLKVNGLGLEVLQKAIEQAEVGRDHILGEMNKAISSVKGNLSENAPRITQKQI
PKDRIGELIGPGGKMIRAIIEQSGSEISVDDSGKVTIASPSEESKEKAIAMIDGIFEEIEVGKIYDGVIKRIADFGAFVE
ILPGKEGLCHISKLDVKRVQSVRDIVSEGDKIKVKVISVDKMGKIDLSRKDVLLDN

Sequences:

>Translated_696_residues
MATEFTGVWGRDSITLETGKWAKQAHGSVVYKTGNLVLLATVCAAEEPKEGQDFFPLTCEYTEKAYSVGRFPGGYFKREA
KPAEHEVLLSRILDRPIRPMFPEGYFSEVQLLVQVLSADKQVSVQGHAINAASAALSVSSIPFAGPIAGARIGRIGGEFI
LNPTNEEITKSDLDLVVAGTKDAIVMIEGEASEISKEDMMAALRFAQEQLKFAVAMQEELAKKHGTVKKEVVLKAPDKDL
HAKIREFAFDRLTLANKNADKAKRNDDIKAINKETVEHFKTLLAPEDKTKDIKHFLHELEYEVVRELVLGEGIRFDGRKT
DEIRQISCEIDVLPGPHGSAVFTRGQTQSLGVMTLGTTSDNQRYETLEGSKEKNFMLHYNFPAFSVGEVRRNSGPGRREI
GHGNLAERAIKKVLPTQTEFPYVIRLVSEILESNGSSSMASVCSGTLALMAGGVPISGAVSGIAMGLFSDEKGRFAVLSD
IAGIEDHFGDMDFKLAGTKKGITAFQMDLKVNGLGLEVLQKAIEQAEVGRDHILGEMNKAISSVKGNLSENAPRITQKQI
PKDRIGELIGPGGKMIRAIIEQSGSEISVDDSGKVTIASPSEESKEKAIAMIDGIFEEIEVGKIYDGVIKRIADFGAFVE
ILPGKEGLCHISKLDVKRVQSVRDIVSEGDKIKVKVISVDKMGKIDLSRKDVLLDN
>Mature_695_residues
ATEFTGVWGRDSITLETGKWAKQAHGSVVYKTGNLVLLATVCAAEEPKEGQDFFPLTCEYTEKAYSVGRFPGGYFKREAK
PAEHEVLLSRILDRPIRPMFPEGYFSEVQLLVQVLSADKQVSVQGHAINAASAALSVSSIPFAGPIAGARIGRIGGEFIL
NPTNEEITKSDLDLVVAGTKDAIVMIEGEASEISKEDMMAALRFAQEQLKFAVAMQEELAKKHGTVKKEVVLKAPDKDLH
AKIREFAFDRLTLANKNADKAKRNDDIKAINKETVEHFKTLLAPEDKTKDIKHFLHELEYEVVRELVLGEGIRFDGRKTD
EIRQISCEIDVLPGPHGSAVFTRGQTQSLGVMTLGTTSDNQRYETLEGSKEKNFMLHYNFPAFSVGEVRRNSGPGRREIG
HGNLAERAIKKVLPTQTEFPYVIRLVSEILESNGSSSMASVCSGTLALMAGGVPISGAVSGIAMGLFSDEKGRFAVLSDI
AGIEDHFGDMDFKLAGTKKGITAFQMDLKVNGLGLEVLQKAIEQAEVGRDHILGEMNKAISSVKGNLSENAPRITQKQIP
KDRIGELIGPGGKMIRAIIEQSGSEISVDDSGKVTIASPSEESKEKAIAMIDGIFEEIEVGKIYDGVIKRIADFGAFVEI
LPGKEGLCHISKLDVKRVQSVRDIVSEGDKIKVKVISVDKMGKIDLSRKDVLLDN

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=704, Percent_Identity=38.6363636363636, Blast_Score=469, Evalue=1e-132,
Organism=Homo sapiens, GI9506689, Length=223, Percent_Identity=26.457399103139, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI145693187, Length=686, Percent_Identity=47.3760932944606, Blast_Score=624, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI115534063, Length=706, Percent_Identity=34.8441926345609, Blast_Score=360, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17535281, Length=79, Percent_Identity=45.5696202531646, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281362905, Length=705, Percent_Identity=36.1702127659575, Blast_Score=447, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651641, Length=705, Percent_Identity=36.1702127659575, Blast_Score=447, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651643, Length=705, Percent_Identity=36.1702127659575, Blast_Score=447, Evalue=1e-125,
Organism=Drosophila melanogaster, GI161079377, Length=650, Percent_Identity=36.4615384615385, Blast_Score=413, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_LEPBA (B0SH22)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962567.1
- ProteinModelPortal:   B0SH22
- GeneID:   6388819
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1476
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   LBIF355278:LBF_1476-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75945; Mature: 75814

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MATEFTGVWGRDSITLETGKWAKQAHGSVVYKTGNLVLLATVCAAEEPKEGQDFFPLTCE
CCCCCCCCCCCCCEEEECCCHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCEEEH
YTEKAYSVGRFPGGYFKREAKPAEHEVLLSRILDRPIRPMFPEGYFSEVQLLVQVLSADK
HHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
QVSVQGHAINAASAALSVSSIPFAGPIAGARIGRIGGEFILNPTNEEITKSDLDLVVAGT
EEEEECCEEEHHHHHEECCCCCCCCCCCCCHHHCCCCEEEECCCCCHHHHCCCCEEEECC
KDAIVMIEGEASEISKEDMMAALRFAQEQLKFAVAMQEELAKKHGTVKKEVVLKAPDKDL
CCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHH
HAKIREFAFDRLTLANKNADKAKRNDDIKAINKETVEHFKTLLAPEDKTKDIKHFLHELE
HHHHHHHHHHHHEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YEVVRELVLGEGIRFDGRKTDEIRQISCEIDVLPGPHGSAVFTRGQTQSLGVMTLGTTSD
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCEEEEECCCCC
NQRYETLEGSKEKNFMLHYNFPAFSVGEVRRNSGPGRREIGHGNLAERAIKKVLPTQTEF
CCHHHHCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
PYVIRLVSEILESNGSSSMASVCSGTLALMAGGVPISGAVSGIAMGLFSDEKGRFAVLSD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEECCCCCCEEEHHH
IAGIEDHFGDMDFKLAGTKKGITAFQMDLKVNGLGLEVLQKAIEQAEVGRDHILGEMNKA
HCCHHHHCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHH
ISSVKGNLSENAPRITQKQIPKDRIGELIGPGGKMIRAIIEQSGSEISVDDSGKVTIASP
HHHHCCCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCEEEECC
SEESKEKAIAMIDGIFEEIEVGKIYDGVIKRIADFGAFVEILPGKEGLCHISKLDVKRVQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEHHHHHHHHHH
SVRDIVSEGDKIKVKVISVDKMGKIDLSRKDVLLDN
HHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATEFTGVWGRDSITLETGKWAKQAHGSVVYKTGNLVLLATVCAAEEPKEGQDFFPLTCE
CCCCCCCCCCCCEEEECCCHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCEEEH
YTEKAYSVGRFPGGYFKREAKPAEHEVLLSRILDRPIRPMFPEGYFSEVQLLVQVLSADK
HHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
QVSVQGHAINAASAALSVSSIPFAGPIAGARIGRIGGEFILNPTNEEITKSDLDLVVAGT
EEEEECCEEEHHHHHEECCCCCCCCCCCCCHHHCCCCEEEECCCCCHHHHCCCCEEEECC
KDAIVMIEGEASEISKEDMMAALRFAQEQLKFAVAMQEELAKKHGTVKKEVVLKAPDKDL
CCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHH
HAKIREFAFDRLTLANKNADKAKRNDDIKAINKETVEHFKTLLAPEDKTKDIKHFLHELE
HHHHHHHHHHHHEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YEVVRELVLGEGIRFDGRKTDEIRQISCEIDVLPGPHGSAVFTRGQTQSLGVMTLGTTSD
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCEEEEECCCCC
NQRYETLEGSKEKNFMLHYNFPAFSVGEVRRNSGPGRREIGHGNLAERAIKKVLPTQTEF
CCHHHHCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
PYVIRLVSEILESNGSSSMASVCSGTLALMAGGVPISGAVSGIAMGLFSDEKGRFAVLSD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEECCCCCCEEEHHH
IAGIEDHFGDMDFKLAGTKKGITAFQMDLKVNGLGLEVLQKAIEQAEVGRDHILGEMNKA
HCCHHHHCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHH
ISSVKGNLSENAPRITQKQIPKDRIGELIGPGGKMIRAIIEQSGSEISVDDSGKVTIASP
HHHHCCCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCEEEECC
SEESKEKAIAMIDGIFEEIEVGKIYDGVIKRIADFGAFVEILPGKEGLCHISKLDVKRVQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEHHHHHHHHHH
SVRDIVSEGDKIKVKVISVDKMGKIDLSRKDVLLDN
HHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA