Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 183220911

GI number: 183220911

Start: 1588275

End: 1589675

Strand: Direct

Name: nusA [H]

Synonym: LEPBI_I1524

Alternate gene names: 183220911

Gene position: 1588275-1589675 (Clockwise)

Preceding gene: 183220910

Following gene: 183220912

Centisome position: 44.12

GC content: 46.04

Gene sequence:

>1401_bases
ATGGCGACAAAACAAGCAACGAAAGAAACTGGGCTATTCGAAGCCATCCAACAATTCTGTCAGGACAAATCCCTTGACAG
AGAACTCGTACTCGGTGTCATCCGCGACTCACTCCTTGCTGCCTATCGCAAAAAAGTTGGTTTGGAAGCTGAGACCGATG
ACCGCTGCCAGGTTGAATTTGGCTCCGATAATAAAAACGAAATCATCATCTCTGTGTTACGCGACGTCGTAGCCGAAAAA
ACAATCAACCCTCTGGAAGTGTCTTTAGAAGATGCACAGAAAATCGATCCAAAGATCGAAGTGGGTAGCCAAATCAGAGT
GTTTGAAAAACCACAAGATTTGTCTCGGGTTCTTTCGAGCCAAGCCAAACAAATGGTCTTCCAACGTTTGCGAGACATGG
AAAAAGAATTACTCTACCAAGAATACAAATCCAAAGAAGGAGAACTCACACACGGGTACTTCCAACGATGGAAAAAGGAC
ATCATGTCCATTGACCTTGGAAAAGTAGAAGGCATCATGCTGAAAAAAGACCAAAACCCTGGTGAAAAATACCGCCAAGG
TGACCGTCTGAAGGCCATTATCTCTCGAGTGGAACTAAGGCCCCGAGAACCAATGCCTGTCATCACACTTTCCCGTGCTT
CTGGCGACTTTGTCAAAAAACTCTTCGAGATGGAAATTCCTGAAGTGTATGATGGCATTGTTGAAATCAGAGATGTTGCA
CGTATCCCGTCTTACAGAACAAAGGTGGTTGTCACTACAAGTAAGTCAGACGTGGATCCTGTGGGTGCTTGTGTGGGGAT
GAAAGGGGTTCGGATCCAAGCCATCGTCAGAGAACTGGGAAACGAAAGGATCGACATCGTCCTTCATTCGGATGAACCAA
GCATTTTCATTGCGAATGCGATTTCCCCGGCAAAACCAGTGGAAGTGCATGTGGACAGAAAACGAGGGGATGCCCTTGTC
ATTGTTCCCGATGAGTCCTTATCGCTTGCGATTGGAATCAATGGTTCCAATGTAAAACTTGTCTCCCAACTATCGGGATT
CAAAATTGATATCAAAACCGTTTCTCAATACAACCAGGAACTGGCTTCTCCCGAAGCACGTGAAAAACTGGACCGACTCT
TCAACGCCCAACAAGAGGCGATGGAAGAATCGGAAGATGGTTACAACCAATCGGGACAAGACGAGGAAGAAGAAGACTCC
GGTTTCACTCCTCTCTCTGAAATCCCAGGTCTCACTCCAAGGATCGTTGGCCTTCTTGAGGCTGGCGGGATCAAAAACGT
GGAAACCCTTCTCGAGTTCAGCCAAGAAGAACTTTCGAAAATTTCCGGAATTGGAAAAACCACGGCAGAACAAATTTTAC
GTCTGTTACGTGAGTCAATCGAATGGGTAGAAGAGGGTTAA

Upstream 100 bases:

>100_bases
CTTTACGATCGTAAAACTCGACACAAAAAGAACCAAAAATCGGTATCTATGCCCATCGCAGAAATACGAAAGGGAAATTT
GTATTTAGAAATTTAAGACT

Downstream 100 bases:

>100_bases
GTTTTAAGGCATAATATGGAAGAGCAAAAATCGATAAAAGAAACCCTCCAGCAGGGAGCCAGTGGTGACAAAACCAAGAA
AAAGCTTGTCATCAAAAAGA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MATKQATKETGLFEAIQQFCQDKSLDRELVLGVIRDSLLAAYRKKVGLEAETDDRCQVEFGSDNKNEIIISVLRDVVAEK
TINPLEVSLEDAQKIDPKIEVGSQIRVFEKPQDLSRVLSSQAKQMVFQRLRDMEKELLYQEYKSKEGELTHGYFQRWKKD
IMSIDLGKVEGIMLKKDQNPGEKYRQGDRLKAIISRVELRPREPMPVITLSRASGDFVKKLFEMEIPEVYDGIVEIRDVA
RIPSYRTKVVVTTSKSDVDPVGACVGMKGVRIQAIVRELGNERIDIVLHSDEPSIFIANAISPAKPVEVHVDRKRGDALV
IVPDESLSLAIGINGSNVKLVSQLSGFKIDIKTVSQYNQELASPEAREKLDRLFNAQQEAMEESEDGYNQSGQDEEEEDS
GFTPLSEIPGLTPRIVGLLEAGGIKNVETLLEFSQEELSKISGIGKTTAEQILRLLRESIEWVEEG

Sequences:

>Translated_466_residues
MATKQATKETGLFEAIQQFCQDKSLDRELVLGVIRDSLLAAYRKKVGLEAETDDRCQVEFGSDNKNEIIISVLRDVVAEK
TINPLEVSLEDAQKIDPKIEVGSQIRVFEKPQDLSRVLSSQAKQMVFQRLRDMEKELLYQEYKSKEGELTHGYFQRWKKD
IMSIDLGKVEGIMLKKDQNPGEKYRQGDRLKAIISRVELRPREPMPVITLSRASGDFVKKLFEMEIPEVYDGIVEIRDVA
RIPSYRTKVVVTTSKSDVDPVGACVGMKGVRIQAIVRELGNERIDIVLHSDEPSIFIANAISPAKPVEVHVDRKRGDALV
IVPDESLSLAIGINGSNVKLVSQLSGFKIDIKTVSQYNQELASPEAREKLDRLFNAQQEAMEESEDGYNQSGQDEEEEDS
GFTPLSEIPGLTPRIVGLLEAGGIKNVETLLEFSQEELSKISGIGKTTAEQILRLLRESIEWVEEG
>Mature_465_residues
ATKQATKETGLFEAIQQFCQDKSLDRELVLGVIRDSLLAAYRKKVGLEAETDDRCQVEFGSDNKNEIIISVLRDVVAEKT
INPLEVSLEDAQKIDPKIEVGSQIRVFEKPQDLSRVLSSQAKQMVFQRLRDMEKELLYQEYKSKEGELTHGYFQRWKKDI
MSIDLGKVEGIMLKKDQNPGEKYRQGDRLKAIISRVELRPREPMPVITLSRASGDFVKKLFEMEIPEVYDGIVEIRDVAR
IPSYRTKVVVTTSKSDVDPVGACVGMKGVRIQAIVRELGNERIDIVLHSDEPSIFIANAISPAKPVEVHVDRKRGDALVI
VPDESLSLAIGINGSNVKLVSQLSGFKIDIKTVSQYNQELASPEAREKLDRLFNAQQEAMEESEDGYNQSGQDEEEEDSG
FTPLSEIPGLTPRIVGLLEAGGIKNVETLLEFSQEELSKISGIGKTTAEQILRLLRESIEWVEEG

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=345, Percent_Identity=33.6231884057971, Blast_Score=206, Evalue=3e-54,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 52307; Mature: 52176

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKQATKETGLFEAIQQFCQDKSLDRELVLGVIRDSLLAAYRKKVGLEAETDDRCQVEF
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
GSDNKNEIIISVLRDVVAEKTINPLEVSLEDAQKIDPKIEVGSQIRVFEKPQDLSRVLSS
CCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCEECCCEEEEECCCHHHHHHHHH
QAKQMVFQRLRDMEKELLYQEYKSKEGELTHGYFQRWKKDIMSIDLGKVEGIMLKKDQNP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
GEKYRQGDRLKAIISRVELRPREPMPVITLSRASGDFVKKLFEMEIPEVYDGIVEIRDVA
CHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHH
RIPSYRTKVVVTTSKSDVDPVGACVGMKGVRIQAIVRELGNERIDIVLHSDEPSIFIANA
HCCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCEEEEEC
ISPAKPVEVHVDRKRGDALVIVPDESLSLAIGINGSNVKLVSQLSGFKIDIKTVSQYNQE
CCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCHHEECCCCCEEEHHHHHHHHHH
LASPEAREKLDRLFNAQQEAMEESEDGYNQSGQDEEEEDSGFTPLSEIPGLTPRIVGLLE
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCHHHCCCCCHHHHHHHH
AGGIKNVETLLEFSQEELSKISGIGKTTAEQILRLLRESIEWVEEG
CCCCCHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATKQATKETGLFEAIQQFCQDKSLDRELVLGVIRDSLLAAYRKKVGLEAETDDRCQVEF
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
GSDNKNEIIISVLRDVVAEKTINPLEVSLEDAQKIDPKIEVGSQIRVFEKPQDLSRVLSS
CCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCEECCCEEEEECCCHHHHHHHHH
QAKQMVFQRLRDMEKELLYQEYKSKEGELTHGYFQRWKKDIMSIDLGKVEGIMLKKDQNP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
GEKYRQGDRLKAIISRVELRPREPMPVITLSRASGDFVKKLFEMEIPEVYDGIVEIRDVA
CHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHH
RIPSYRTKVVVTTSKSDVDPVGACVGMKGVRIQAIVRELGNERIDIVLHSDEPSIFIANA
HCCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCEEEEEC
ISPAKPVEVHVDRKRGDALVIVPDESLSLAIGINGSNVKLVSQLSGFKIDIKTVSQYNQE
CCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCHHEECCCCCEEEHHHHHHHHHH
LASPEAREKLDRLFNAQQEAMEESEDGYNQSGQDEEEEDSGFTPLSEIPGLTPRIVGLLE
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCHHHCCCCCHHHHHHHH
AGGIKNVETLLEFSQEELSKISGIGKTTAEQILRLLRESIEWVEEG
CCCCCHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]