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The map label for this gene is fabI [H]

Identifier: 183220591

GI number: 183220591

Start: 1246420

End: 1247256

Strand: Direct

Name: fabI [H]

Synonym: LEPBI_I1195

Alternate gene names: 183220591

Gene position: 1246420-1247256 (Clockwise)

Preceding gene: 183220590

Following gene: 183220593

Centisome position: 34.63

GC content: 41.22

Gene sequence:

>837_bases
ATGAACCAAACTCTAAAAGGTCGTACTGTGATCATTACTGGCATCACGGATGCGTCTTCACTTGCACTCATCATTGCAAA
AGAATGTAAAGACCAAGGTGCCAAACTCATTTGTACAGGTCTTGGCAAAACACCGTTTCACAAAAATTTATCTGAGAATA
GTATCAATTTTTTAGATCGAACTTACTCGGATTTCCAAAATACAGTTAAGCAAGAGTTAGGTGAAGATGTGGTTACTTTT
CCATTAGATGTTACCATCCAAGAAAGTATCGATTCCTTTGCTGATTTTCTCCTAGAAAAAAAGGAAACCATTCATTCCTT
ACTTCATTCCATTGCGATGGATAAAACCATTCGCCAAGGAAAAGTAAAACCCATAATGACCGTCTCACGAGAAGAATTTA
TGGATGCCATGAATGTATCTTCCTTTTCACTCCTTGCTATCGTACAAAGCCTATACAATCGTAACCTCTTAGTGAAAGGA
GCATCGATTGTGGCACTCAGTTATTTGGGTGCTGAAAAAGTGGTAATCCACCCTTATAAAAACATTGGTGTCGCCAAAGC
GGCGTTAGAGAGACTTGTGAAAGAAATGGCGATGGAGTTAGGGAAAGAAAAGGAGATCCAAGTGAATGCGATACGATTTT
CTCCGTACCGTGCAAGTAAAGCAGGTTCTGCCATCGAGGGATTAGAGGAAGCAGAGATTCATTGCCATACCTCTGCACCA
CTCGGGAATGCACTTGCAAAAGACCTGGCGGAAGAAGTATGTTATTTGTTCCGCCCATCCAATCGGATCACAGGTGAAAT
TCGTCATGTGGATGGAGGGTATCACATCAGAGGATAA

Upstream 100 bases:

>100_bases
CTTACGAAAAAATTCAGCACAAACGATAAAAAAAACCTGAAAAAAAGTTCGTCTTTTAAAGCACCTCCGAAACTTCTGTG
TCTAAGCACTAGGAGGAGAT

Downstream 100 bases:

>100_bases
AATTTAAGTAGAGGGTGGTCCAAAGTGTTCCTTAACAAGTGTAACAAAATGGTTCCCACGCTCTTCAAAATTTTTATATA
GATCAAAACTAGCCCCTGCC

Product: putative enoyl-(Acyl-carrier-protein) reductase

Products: NA

Alternate protein names: Cold shock-induced protein 15; CSI15; NADH-dependent enoyl-ACP reductase; Vegetative protein 241; VEG241 [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNQTLKGRTVIITGITDASSLALIIAKECKDQGAKLICTGLGKTPFHKNLSENSINFLDRTYSDFQNTVKQELGEDVVTF
PLDVTIQESIDSFADFLLEKKETIHSLLHSIAMDKTIRQGKVKPIMTVSREEFMDAMNVSSFSLLAIVQSLYNRNLLVKG
ASIVALSYLGAEKVVIHPYKNIGVAKAALERLVKEMAMELGKEKEIQVNAIRFSPYRASKAGSAIEGLEEAEIHCHTSAP
LGNALAKDLAEEVCYLFRPSNRITGEIRHVDGGYHIRG

Sequences:

>Translated_278_residues
MNQTLKGRTVIITGITDASSLALIIAKECKDQGAKLICTGLGKTPFHKNLSENSINFLDRTYSDFQNTVKQELGEDVVTF
PLDVTIQESIDSFADFLLEKKETIHSLLHSIAMDKTIRQGKVKPIMTVSREEFMDAMNVSSFSLLAIVQSLYNRNLLVKG
ASIVALSYLGAEKVVIHPYKNIGVAKAALERLVKEMAMELGKEKEIQVNAIRFSPYRASKAGSAIEGLEEAEIHCHTSAP
LGNALAKDLAEEVCYLFRPSNRITGEIRHVDGGYHIRG
>Mature_278_residues
MNQTLKGRTVIITGITDASSLALIIAKECKDQGAKLICTGLGKTPFHKNLSENSINFLDRTYSDFQNTVKQELGEDVVTF
PLDVTIQESIDSFADFLLEKKETIHSLLHSIAMDKTIRQGKVKPIMTVSREEFMDAMNVSSFSLLAIVQSLYNRNLLVKG
ASIVALSYLGAEKVVIHPYKNIGVAKAALERLVKEMAMELGKEKEIQVNAIRFSPYRASKAGSAIEGLEEAEIHCHTSAP
LGNALAKDLAEEVCYLFRPSNRITGEIRHVDGGYHIRG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=274, Percent_Identity=30.6569343065693, Blast_Score=98, Evalue=7e-22,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 30669; Mature: 30669

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTLKGRTVIITGITDASSLALIIAKECKDQGAKLICTGLGKTPFHKNLSENSINFLDR
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHH
TYSDFQNTVKQELGEDVVTFPLDVTIQESIDSFADFLLEKKETIHSLLHSIAMDKTIRQG
HHHHHHHHHHHHHCCCEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KVKPIMTVSREEFMDAMNVSSFSLLAIVQSLYNRNLLVKGASIVALSYLGAEKVVIHPYK
CCCHHHEECHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECHHEEEEECCCCCCEEEECCC
NIGVAKAALERLVKEMAMELGKEKEIQVNAIRFSPYRASKAGSAIEGLEEAEIHCHTSAP
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHCCHHCCEEEECCCC
LGNALAKDLAEEVCYLFRPSNRITGEIRHVDGGYHIRG
CHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEECC
>Mature Secondary Structure
MNQTLKGRTVIITGITDASSLALIIAKECKDQGAKLICTGLGKTPFHKNLSENSINFLDR
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHH
TYSDFQNTVKQELGEDVVTFPLDVTIQESIDSFADFLLEKKETIHSLLHSIAMDKTIRQG
HHHHHHHHHHHHHCCCEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KVKPIMTVSREEFMDAMNVSSFSLLAIVQSLYNRNLLVKGASIVALSYLGAEKVVIHPYK
CCCHHHEECHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECHHEEEEECCCCCCEEEECCC
NIGVAKAALERLVKEMAMELGKEKEIQVNAIRFSPYRASKAGSAIEGLEEAEIHCHTSAP
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHCCHHCCEEEECCCC
LGNALAKDLAEEVCYLFRPSNRITGEIRHVDGGYHIRG
CHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 8755892; 9298659 [H]