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The map label for this gene is mdh

Identifier: 183220541

GI number: 183220541

Start: 1192329

End: 1193312

Strand: Direct

Name: mdh

Synonym: LEPBI_I1144

Alternate gene names: 183220541

Gene position: 1192329-1193312 (Clockwise)

Preceding gene: 183220540

Following gene: 183220543

Centisome position: 33.12

GC content: 43.9

Gene sequence:

>984_bases
ATGAGCAAAAAAGTGAAAGTTGCTGTAACAGGTGCAGCCGGACAAATCGGATATGCACTTTTATTTCGTATCGCTTCAGG
ACAAATGTTCGGACCTGACACGGCCGTAGAACTCCAGTTATTGGAACTAGAACAAGCAATCCCTGCGGCAAAAGGTGTCA
TCATGGAATTGGATGACTGTGCATTTCCTTTACTTGAAAAAGTATCCGTATCATCTAATATTGATGAAGCATTCCGAGAC
ATCAACTGGGCACTCCTCGTGGGATCCGTTCCAAGGAAAGCCGGTATGGAACGTGGGGACCTTCTTAAAATCAATGGTGG
TATTTTTACAACCCAAGGGAAAGCGATCGAAAAAAATGCTGCAAGTGATGTAAGAGTTCTCGTAGTTGGAAACCCTTGTA
ACACAAACGCACTCATTGCTATGAACAATGCAAAAGGTGTTCCGTCTGACAGATGGTTTGCGATGACTGGTCTTGATGAA
AACCGTGCAAAAACACAATTGGCACAGAAAGCTGGTGTTCTCGTAAAAGACGTATCCAACGTTGCGATTTGGGGGAACCA
CTCTGCCACCCAATACCCTGACTTTTTTAATGCAAAAGTGAATGGAAAACCGGCAACTGACGTGATCAGTGACCACGATT
GGCTAAAAGGAGATTTCATCTCTACCGTACAAAAACGTGGTGCGGCCATCATTGCTGCAAGAGGTGCTTCTTCTGCGGCG
TCTGCTGCCAATGCAGTGGTAGACACGGTGCATAACATTGTCACTCCAACAAAACCTGGCGACTGGTTCAGTGCTGCTTG
CCACTCTAATGGTGAATACGGTGTGGACAAAGGCCTAATCTTTGGATACCCTTTGAAGTCCGATGGAAAAAAAGTAGAGA
TCGTAACTGGTCTTGAAATCAATGCCTTTGGTAAGGAAAAATTTGACATCACTCACAATGAGTTAAAAGAAGAAAGAAAC
GAAGTAAAAGATATGTTAGGTTAA

Upstream 100 bases:

>100_bases
GGATCTTTGTATCAGGTGCCTTACCATTTGAAAACTTCGATGAGATCATCCAAAAAGAACTGAAACAATAAAGGATTATA
AACCAAAAAGGAAAAATTAT

Downstream 100 bases:

>100_bases
GAGAAAAAAACCTATCTTTCCTATAGAATTTTTTTATAGGGAATTTGTTTCCTAAAACAAAACCCACTTGGCACTCTTTG
TTCCAGTGGGTTTTTTTATA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSKKVKVAVTGAAGQIGYALLFRIASGQMFGPDTAVELQLLELEQAIPAAKGVIMELDDCAFPLLEKVSVSSNIDEAFRD
INWALLVGSVPRKAGMERGDLLKINGGIFTTQGKAIEKNAASDVRVLVVGNPCNTNALIAMNNAKGVPSDRWFAMTGLDE
NRAKTQLAQKAGVLVKDVSNVAIWGNHSATQYPDFFNAKVNGKPATDVISDHDWLKGDFISTVQKRGAAIIAARGASSAA
SAANAVVDTVHNIVTPTKPGDWFSAACHSNGEYGVDKGLIFGYPLKSDGKKVEIVTGLEINAFGKEKFDITHNELKEERN
EVKDMLG

Sequences:

>Translated_327_residues
MSKKVKVAVTGAAGQIGYALLFRIASGQMFGPDTAVELQLLELEQAIPAAKGVIMELDDCAFPLLEKVSVSSNIDEAFRD
INWALLVGSVPRKAGMERGDLLKINGGIFTTQGKAIEKNAASDVRVLVVGNPCNTNALIAMNNAKGVPSDRWFAMTGLDE
NRAKTQLAQKAGVLVKDVSNVAIWGNHSATQYPDFFNAKVNGKPATDVISDHDWLKGDFISTVQKRGAAIIAARGASSAA
SAANAVVDTVHNIVTPTKPGDWFSAACHSNGEYGVDKGLIFGYPLKSDGKKVEIVTGLEINAFGKEKFDITHNELKEERN
EVKDMLG
>Mature_326_residues
SKKVKVAVTGAAGQIGYALLFRIASGQMFGPDTAVELQLLELEQAIPAAKGVIMELDDCAFPLLEKVSVSSNIDEAFRDI
NWALLVGSVPRKAGMERGDLLKINGGIFTTQGKAIEKNAASDVRVLVVGNPCNTNALIAMNNAKGVPSDRWFAMTGLDEN
RAKTQLAQKAGVLVKDVSNVAIWGNHSATQYPDFFNAKVNGKPATDVISDHDWLKGDFISTVQKRGAAIIAARGASSAAS
AANAVVDTVHNIVTPTKPGDWFSAACHSNGEYGVDKGLIFGYPLKSDGKKVEIVTGLEINAFGKEKFDITHNELKEERNE
VKDMLG

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=331, Percent_Identity=48.036253776435, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI89886456, Length=308, Percent_Identity=26.6233766233766, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17561064, Length=326, Percent_Identity=47.5460122699387, Blast_Score=271, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI32566798, Length=257, Percent_Identity=49.4163424124514, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI32566800, Length=172, Percent_Identity=47.093023255814, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324446, Length=271, Percent_Identity=28.7822878228782, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583394, Length=330, Percent_Identity=48.4848484848485, Blast_Score=298, Evalue=4e-81,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_LEPBA (B0SF41)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962206.1
- ProteinModelPortal:   B0SF41
- SMR:   B0SF41
- GeneID:   6388414
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1102
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   LBIF355278:LBF_1102-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34931; Mature: 34800

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 187-187 BINDING 92-92 BINDING 98-98 BINDING 105-105 BINDING 112-112 BINDING 131-131 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKVKVAVTGAAGQIGYALLFRIASGQMFGPDTAVELQLLELEQAIPAAKGVIMELDDC
CCCEEEEEEECCCCHHHHHHHHHHHCCCEECCCCCEEEEEEHHHHHCCHHCCEEEEECCH
AFPLLEKVSVSSNIDEAFRDINWALLVGSVPRKAGMERGDLLKINGGIFTTQGKAIEKNA
HHHHHHHHHCCCCHHHHHHCCCEEEEEECCCHHCCCCCCCEEEECCCEEECCCCCCCCCC
ASDVRVLVVGNPCNTNALIAMNNAKGVPSDRWFAMTGLDENRAKTQLAQKAGVLVKDVSN
CCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEECCC
VAIWGNHSATQYPDFFNAKVNGKPATDVISDHDWLKGDFISTVQKRGAAIIAARGASSAA
EEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCHH
SAANAVVDTVHNIVTPTKPGDWFSAACHSNGEYGVDKGLIFGYPLKSDGKKVEIVTGLEI
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCEEEEEECCEE
NAFGKEKFDITHNELKEERNEVKDMLG
EECCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKKVKVAVTGAAGQIGYALLFRIASGQMFGPDTAVELQLLELEQAIPAAKGVIMELDDC
CCEEEEEEECCCCHHHHHHHHHHHCCCEECCCCCEEEEEEHHHHHCCHHCCEEEEECCH
AFPLLEKVSVSSNIDEAFRDINWALLVGSVPRKAGMERGDLLKINGGIFTTQGKAIEKNA
HHHHHHHHHCCCCHHHHHHCCCEEEEEECCCHHCCCCCCCEEEECCCEEECCCCCCCCCC
ASDVRVLVVGNPCNTNALIAMNNAKGVPSDRWFAMTGLDENRAKTQLAQKAGVLVKDVSN
CCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEECCC
VAIWGNHSATQYPDFFNAKVNGKPATDVISDHDWLKGDFISTVQKRGAAIIAARGASSAA
EEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCHH
SAANAVVDTVHNIVTPTKPGDWFSAACHSNGEYGVDKGLIFGYPLKSDGKKVEIVTGLEI
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCEEEEEECCEE
NAFGKEKFDITHNELKEERNEVKDMLG
EECCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA