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The map label for this gene is paaF [C]

Identifier: 183220275

GI number: 183220275

Start: 889678

End: 890496

Strand: Direct

Name: paaF [C]

Synonym: LEPBI_I0870

Alternate gene names: 183220275

Gene position: 889678-890496 (Clockwise)

Preceding gene: 183220274

Following gene: 183220276

Centisome position: 24.72

GC content: 39.56

Gene sequence:

>819_bases
ATGAAATCGCGATTTGAATCCAAAGAGTATGAATTCCTAGAAATAGAATCCCGTGAAACAGAAGATGGGACCATTCTTTC
CATCTTCTTAAACAATCCCTCGTCTCGAAATTCCATGACATGGAAAATGGGAGAAGAGTTCGCTGACCTCATCCATTCCA
TTCGCAAACAAAAAAAACTTCCAAGAGTTGTCATTGTTTCAGGAAGGAATGATGTGTTTTGTGCCGGTGGTGATCTGAAT
TTATTACGTTCTTTCTCTGAAAAAAGTTTCTCACAAAACAGGCGTGATATGAGAAAGTTTTATGGTTTTTTCTTGTCTGT
TAGAAAACTTCCAGTGCCTGTGATTGCGGCGGTGAATGGTCATGCGATTGGAGCAGGTCTTTCATTAACCTTTGGTTGTG
ACTTACGAATTTATGCAAGTGAAGGAAAGTATTCATTTAATTTTGTGCGTCTTGGAATCCATCCTGGAATGGGCTCTAGT
TTTTTAGCTCCTGAACTTCTCGGAAAAAGTTTGGGTGGAAGGTTACTCATCACGGGAGAAACCTTCGATGGAAACAAGGC
AAAAGACTGGGGTCTTGCACTGGATTCTGTTGCAAAACATTCCGTATATGAACGAGCCATGGAACTGGCCATCTCTTTGT
CTAAGGCATCTCCACTTGCTTTACAAGAACTCAAAACAAATCTGTATTCATGGAAACAACTCGATTCTGCATTGAAAAAA
GAAGCAGAATCCCAAGCGCGAAATTTTATCTCGGATGACTTTAAAGAGACCATTCAGAGTATTATAGAAAAGCGAGATCC
TAAATTTAAGGGAAAATAA

Upstream 100 bases:

>100_bases
CAACATTTAGAAATTTTACTCAATTACTTTGAAAATATGGAGATTGGGAACAAACCTTTATCTTTACAAGATAAAGCCTG
GTACGAATCCTTTCAGATAG

Downstream 100 bases:

>100_bases
AATTTTAGAAAGTTTCTAAAATTTTGTCTCGACAAGCGACTGGTAAGGGGTTACATAAAAGTTTGATGTCTGACCTTCCA
CTCATTCCCGATTGTTTTTT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKSRFESKEYEFLEIESRETEDGTILSIFLNNPSSRNSMTWKMGEEFADLIHSIRKQKKLPRVVIVSGRNDVFCAGGDLN
LLRSFSEKSFSQNRRDMRKFYGFFLSVRKLPVPVIAAVNGHAIGAGLSLTFGCDLRIYASEGKYSFNFVRLGIHPGMGSS
FLAPELLGKSLGGRLLITGETFDGNKAKDWGLALDSVAKHSVYERAMELAISLSKASPLALQELKTNLYSWKQLDSALKK
EAESQARNFISDDFKETIQSIIEKRDPKFKGK

Sequences:

>Translated_272_residues
MKSRFESKEYEFLEIESRETEDGTILSIFLNNPSSRNSMTWKMGEEFADLIHSIRKQKKLPRVVIVSGRNDVFCAGGDLN
LLRSFSEKSFSQNRRDMRKFYGFFLSVRKLPVPVIAAVNGHAIGAGLSLTFGCDLRIYASEGKYSFNFVRLGIHPGMGSS
FLAPELLGKSLGGRLLITGETFDGNKAKDWGLALDSVAKHSVYERAMELAISLSKASPLALQELKTNLYSWKQLDSALKK
EAESQARNFISDDFKETIQSIIEKRDPKFKGK
>Mature_272_residues
MKSRFESKEYEFLEIESRETEDGTILSIFLNNPSSRNSMTWKMGEEFADLIHSIRKQKKLPRVVIVSGRNDVFCAGGDLN
LLRSFSEKSFSQNRRDMRKFYGFFLSVRKLPVPVIAAVNGHAIGAGLSLTFGCDLRIYASEGKYSFNFVRLGIHPGMGSS
FLAPELLGKSLGGRLLITGETFDGNKAKDWGLALDSVAKHSVYERAMELAISLSKASPLALQELKTNLYSWKQLDSALKK
EAESQARNFISDDFKETIQSIIEKRDPKFKGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4502327, Length=273, Percent_Identity=27.8388278388278, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI194097323, Length=265, Percent_Identity=26.7924528301887, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI31542718, Length=269, Percent_Identity=26.7657992565056, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI70995211, Length=271, Percent_Identity=27.6752767527675, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI20127408, Length=174, Percent_Identity=25.2873563218391, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI45643119, Length=226, Percent_Identity=27.4336283185841, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI260275230, Length=226, Percent_Identity=27.4336283185841, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI260274832, Length=226, Percent_Identity=27.4336283185841, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI213417737, Length=235, Percent_Identity=25.9574468085106, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157694516, Length=235, Percent_Identity=25.9574468085106, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI25453481, Length=210, Percent_Identity=24.2857142857143, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI51538573, Length=210, Percent_Identity=24.2857142857143, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=29.0836653386454, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=28.7878787878788, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=29.0836653386454, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788682, Length=204, Percent_Identity=30.3921568627451, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788597, Length=260, Percent_Identity=23.4615384615385, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=27.3743016759777, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=261, Percent_Identity=29.1187739463602, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=28.2608695652174, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=26.9387755102041, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=277, Percent_Identity=29.2418772563177, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=224, Percent_Identity=23.6607142857143, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=195, Percent_Identity=25.1282051282051, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=248, Percent_Identity=22.9838709677419, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17534483, Length=276, Percent_Identity=23.1884057971014, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=190, Percent_Identity=24.7368421052632, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=31.7460317460317, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI20129971, Length=275, Percent_Identity=26.5454545454545, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24653477, Length=275, Percent_Identity=26.5454545454545, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19922422, Length=257, Percent_Identity=25.6809338521401, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357171, Length=236, Percent_Identity=26.6949152542373, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24654903, Length=170, Percent_Identity=27.6470588235294, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920382, Length=261, Percent_Identity=26.0536398467433, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30552; Mature: 30552

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRFESKEYEFLEIESRETEDGTILSIFLNNPSSRNSMTWKMGEEFADLIHSIRKQKKL
CCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHCC
PRVVIVSGRNDVFCAGGDLNLLRSFSEKSFSQNRRDMRKFYGFFLSVRKLPVPVIAAVNG
CEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
HAIGAGLSLTFGCDLRIYASEGKYSFNFVRLGIHPGMGSSFLAPELLGKSLGGRLLITGE
CEEECCEEEEECCEEEEEECCCCEEEEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEC
TFDGNKAKDWGLALDSVAKHSVYERAMELAISLSKASPLALQELKTNLYSWKQLDSALKK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EAESQARNFISDDFKETIQSIIEKRDPKFKGK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSRFESKEYEFLEIESRETEDGTILSIFLNNPSSRNSMTWKMGEEFADLIHSIRKQKKL
CCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHCC
PRVVIVSGRNDVFCAGGDLNLLRSFSEKSFSQNRRDMRKFYGFFLSVRKLPVPVIAAVNG
CEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
HAIGAGLSLTFGCDLRIYASEGKYSFNFVRLGIHPGMGSSFLAPELLGKSLGGRLLITGE
CEEECCEEEEECCEEEEEECCCCEEEEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEC
TFDGNKAKDWGLALDSVAKHSVYERAMELAISLSKASPLALQELKTNLYSWKQLDSALKK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EAESQARNFISDDFKETIQSIIEKRDPKFKGK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA