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The map label for this gene is yibK [C]

Identifier: 183220250

GI number: 183220250

Start: 856054

End: 856521

Strand: Direct

Name: yibK [C]

Synonym: LEPBI_I0843

Alternate gene names: 183220250

Gene position: 856054-856521 (Clockwise)

Preceding gene: 183220249

Following gene: 183220251

Centisome position: 23.78

GC content: 45.3

Gene sequence:

>468_bases
TTGGAAATTGCACTGTTTCGTCCCGAAATTCCACCTAACACTGGTAACATAGCGCGCCTTTGTGTGAATGCTGGTGTGCC
ACTTTCCATTGTGGGGGAACCTGCCTTTGATCTCTCTGAAAAAGCAGTCCGGCGGGCAGGGCTTGACTATTGGAAGGATT
TAGAGCTCCATCGATATCCCGATTGGGATTGGTTCCAGAAGGAGAAAGAAACCCAAGGGAAACGAATCTTTCTTGTTTCC
AAGTTTGGGAAAAAAGTTTACTGGGATGTTTCTTTTTCTTCTAAAGATGTGTTCTTGTTTGGCAGGGAGACCTCAGGTTT
ACCAGAAGAAATTCACAAAGGACACCCTTCTGACCAAATCATTTCGATTCCCATGGCAGAATCGAGTCGCTCCATCAATT
TGTCCAATGCAGTTGCCATCATCCTTTATGAAGCACTGCGCCAAGAAAAAACACGGACTATCCCCTAA

Upstream 100 bases:

>100_bases
GATTACCTTTCCAAAGATGATGCCACTACTTCTGTTTCTAGTTTTGGTCTTGCATTGAAACAGTCTTTGGAAAACCAAAA
GAAAAAGAAGTAAGTTCCCT

Downstream 100 bases:

>100_bases
AGGATAAAACAATTTACAACTCCTCCGTTCCCATTGAAATGGGAATATATGCCGTTCCCTCTCTCCAGAACTGAGTTAGA
GAAAGAAATCAAAACTTTGT

Product: SpoU family tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MEIALFRPEIPPNTGNIARLCVNAGVPLSIVGEPAFDLSEKAVRRAGLDYWKDLELHRYPDWDWFQKEKETQGKRIFLVS
KFGKKVYWDVSFSSKDVFLFGRETSGLPEEIHKGHPSDQIISIPMAESSRSINLSNAVAIILYEALRQEKTRTIP

Sequences:

>Translated_155_residues
MEIALFRPEIPPNTGNIARLCVNAGVPLSIVGEPAFDLSEKAVRRAGLDYWKDLELHRYPDWDWFQKEKETQGKRIFLVS
KFGKKVYWDVSFSSKDVFLFGRETSGLPEEIHKGHPSDQIISIPMAESSRSINLSNAVAIILYEALRQEKTRTIP
>Mature_155_residues
MEIALFRPEIPPNTGNIARLCVNAGVPLSIVGEPAFDLSEKAVRRAGLDYWKDLELHRYPDWDWFQKEKETQGKRIFLVS
KFGKKVYWDVSFSSKDVFLFGRETSGLPEEIHKGHPSDQIISIPMAESSRSINLSNAVAIILYEALRQEKTRTIP

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=148, Percent_Identity=44.5945945945946, Blast_Score=127, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17691; Mature: 17691

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIALFRPEIPPNTGNIARLCVNAGVPLSIVGEPAFDLSEKAVRRAGLDYWKDLELHRYP
CCEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCHHHCCCEEECC
DWDWFQKEKETQGKRIFLVSKFGKKVYWDVSFSSKDVFLFGRETSGLPEEIHKGHPSDQI
CCHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCHHHHCCCCCCCE
ISIPMAESSRSINLSNAVAIILYEALRQEKTRTIP
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEIALFRPEIPPNTGNIARLCVNAGVPLSIVGEPAFDLSEKAVRRAGLDYWKDLELHRYP
CCEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCHHHCCCEEECC
DWDWFQKEKETQGKRIFLVSKFGKKVYWDVSFSSKDVFLFGRETSGLPEEIHKGHPSDQI
CCHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCHHHHCCCCCCCE
ISIPMAESSRSINLSNAVAIILYEALRQEKTRTIP
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]