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The map label for this gene is bioB

Identifier: 183220235

GI number: 183220235

Start: 841130

End: 842185

Strand: Direct

Name: bioB

Synonym: LEPBI_I0828

Alternate gene names: 183220235

Gene position: 841130-842185 (Clockwise)

Preceding gene: 183220234

Following gene: 183220236

Centisome position: 23.37

GC content: 42.71

Gene sequence:

>1056_bases
ATGATTGCAGAAGTCCAAGAAAAAACTATCTCTTCCTCTCCCTCTATCATTTCCGAGGAAGAAGCCCTTCAGATTCTCAA
AGGGGAAGTCCCACTTTTACCCGTGGTAGCAAAGGCTTCGGAAGAACGATTTCGTCATTTTGGAAACCGAGTTCGGATTC
ATATCTTAGATAATATCAAAAACGGATACTGCCCTGAAGATTGTGGTTACTGTGCCCAAAGGAAGGGTGGTGAATCTGGG
ATCCAAGAGTATTCTTTAAAATCACCAGAGGAAATTTGGGAAGATGCCAAAAAAGCCAAAGAGAATGGTGCCTACCGATT
CTGTATGGTCACTTCAGGCCGAGGCCCAACGGATAAAGCGGTCGATAAATTGGCAGAGACCATTTCCAAAATCAATGGGG
AACTGGGGATGAAGGTCTGTCTTTCTGCAGGAATCTTAGATGGCAAAAAAGCAAAAACCTTAAAAGACGCAGGACTTGAT
CGATACAATCACAACCTCAATACATCGGAATCCAAATACAACGAAATTTGTTCGACTCATACCTTTAAAGACCGACTAAC
AACACTCGAGGCGGCAAAAGAAGCTGAGATTGGTTTGTGTTCCGGGATCATTGTGGGAATGGGGGAAGAGCTAAAAGACA
TCGTCCAAGTTGCCTTTGAACTAAAAAGGTTGGGTGTGATCTCCATCCCAGTCAACTTTTTTATCCCCATCAAAGGCCAT
GCCATTCAAAAGTCAACACTCACACCTGAATTTTGTGTCCGAGTTTTGTCTGTGTTTCGTTTGGTAAATCCTGATTCTGA
GATCCGTGTGGGTGCCGGTCGTGAAGGGCATTTGGGTTTCTTACAATCAATGGCTCTTTATGTTGCCAATTCCCTCTTTG
CAGAAGGGTATTTGAATGTGAAAGGAAGCGAAATGGAACAAACCATGAATTTGATTCGTGACTGTAATATGGTACCAGAG
TTCACTGAAGGAATTCCTGAAGGATGGGAAGACTACGATTCCAAGTTTTTATACGACGAGAAAAACTTTCCGGAACTCTA
TAAACATAAAAAGTAG

Upstream 100 bases:

>100_bases
GGAAACGACACTGCTTGGATTTTTAGAACCAGAAAAATACTAAAACTCCCATTCCATTTTCTTCGTTTACAGAGTCACCC
ACGCAAAAGATTTTGTTCTC

Downstream 100 bases:

>100_bases
TTTGCCTTTTTTACTTTTGGCGGTATCATGCTCCTACTTTACCGCCTCTAGTGAAACACATGCAAAAACCGTCTTTACCA
TTTGGAAAACAAATTAGTTA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MIAEVQEKTISSSPSIISEEEALQILKGEVPLLPVVAKASEERFRHFGNRVRIHILDNIKNGYCPEDCGYCAQRKGGESG
IQEYSLKSPEEIWEDAKKAKENGAYRFCMVTSGRGPTDKAVDKLAETISKINGELGMKVCLSAGILDGKKAKTLKDAGLD
RYNHNLNTSESKYNEICSTHTFKDRLTTLEAAKEAEIGLCSGIIVGMGEELKDIVQVAFELKRLGVISIPVNFFIPIKGH
AIQKSTLTPEFCVRVLSVFRLVNPDSEIRVGAGREGHLGFLQSMALYVANSLFAEGYLNVKGSEMEQTMNLIRDCNMVPE
FTEGIPEGWEDYDSKFLYDEKNFPELYKHKK

Sequences:

>Translated_351_residues
MIAEVQEKTISSSPSIISEEEALQILKGEVPLLPVVAKASEERFRHFGNRVRIHILDNIKNGYCPEDCGYCAQRKGGESG
IQEYSLKSPEEIWEDAKKAKENGAYRFCMVTSGRGPTDKAVDKLAETISKINGELGMKVCLSAGILDGKKAKTLKDAGLD
RYNHNLNTSESKYNEICSTHTFKDRLTTLEAAKEAEIGLCSGIIVGMGEELKDIVQVAFELKRLGVISIPVNFFIPIKGH
AIQKSTLTPEFCVRVLSVFRLVNPDSEIRVGAGREGHLGFLQSMALYVANSLFAEGYLNVKGSEMEQTMNLIRDCNMVPE
FTEGIPEGWEDYDSKFLYDEKNFPELYKHKK
>Mature_351_residues
MIAEVQEKTISSSPSIISEEEALQILKGEVPLLPVVAKASEERFRHFGNRVRIHILDNIKNGYCPEDCGYCAQRKGGESG
IQEYSLKSPEEIWEDAKKAKENGAYRFCMVTSGRGPTDKAVDKLAETISKINGELGMKVCLSAGILDGKKAKTLKDAGLD
RYNHNLNTSESKYNEICSTHTFKDRLTTLEAAKEAEIGLCSGIIVGMGEELKDIVQVAFELKRLGVISIPVNFFIPIKGH
AIQKSTLTPEFCVRVLSVFRLVNPDSEIRVGAGREGHLGFLQSMALYVANSLFAEGYLNVKGSEMEQTMNLIRDCNMVPE
FTEGIPEGWEDYDSKFLYDEKNFPELYKHKK

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=300, Percent_Identity=34.6666666666667, Blast_Score=181, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6321725, Length=257, Percent_Identity=33.4630350194552, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_LEPBA (B0SDC5)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961908.1
- ProteinModelPortal:   B0SDC5
- SMR:   B0SDC5
- GeneID:   6390164
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0798
- HOGENOM:   HBG405569
- OMA:   LEICSGG
- ProtClustDB:   CLSK574240
- BioCyc:   LBIF355278:LBF_0798-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 39131; Mature: 39131

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAEVQEKTISSSPSIISEEEALQILKGEVPLLPVVAKASEERFRHFGNRVRIHILDNIK
CCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCC
NGYCPEDCGYCAQRKGGESGIQEYSLKSPEEIWEDAKKAKENGAYRFCMVTSGRGPTDKA
CCCCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHH
VDKLAETISKINGELGMKVCLSAGILDGKKAKTLKDAGLDRYNHNLNTSESKYNEICSTH
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHH
TFKDRLTTLEAAKEAEIGLCSGIIVGMGEELKDIVQVAFELKRLGVISIPVNFFIPIKGH
HHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEECCEEEEECCCC
AIQKSTLTPEFCVRVLSVFRLVNPDSEIRVGAGREGHLGFLQSMALYVANSLFAEGYLNV
EECCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
KGSEMEQTMNLIRDCNMVPEFTEGIPEGWEDYDSKFLYDEKNFPELYKHKK
CCHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHCCCEEECCCCCHHHHCCCC
>Mature Secondary Structure
MIAEVQEKTISSSPSIISEEEALQILKGEVPLLPVVAKASEERFRHFGNRVRIHILDNIK
CCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCC
NGYCPEDCGYCAQRKGGESGIQEYSLKSPEEIWEDAKKAKENGAYRFCMVTSGRGPTDKA
CCCCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHH
VDKLAETISKINGELGMKVCLSAGILDGKKAKTLKDAGLDRYNHNLNTSESKYNEICSTH
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHH
TFKDRLTTLEAAKEAEIGLCSGIIVGMGEELKDIVQVAFELKRLGVISIPVNFFIPIKGH
HHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEECCEEEEECCCC
AIQKSTLTPEFCVRVLSVFRLVNPDSEIRVGAGREGHLGFLQSMALYVANSLFAEGYLNV
EECCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
KGSEMEQTMNLIRDCNMVPEFTEGIPEGWEDYDSKFLYDEKNFPELYKHKK
CCHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHCCCEEECCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA