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The map label for this gene is carA [H]

Identifier: 183220223

GI number: 183220223

Start: 830549

End: 831673

Strand: Reverse

Name: carA [H]

Synonym: LEPBI_I0815

Alternate gene names: 183220223

Gene position: 831673-830549 (Counterclockwise)

Preceding gene: 183220224

Following gene: 183220222

Centisome position: 23.1

GC content: 42.31

Gene sequence:

>1125_bases
TTGTTCGCAAGCCTGGCCCAATTCCTTGGAATGGATTTGATGCAGGCTTTTTTGGTTTTAGCAAACGGAACGGTCATGAA
GGGCCGATCCTTCGGTGCAAATAAGAATTCGATCGGAGAGGTAGTTTTTAATACCTCCATGGCAGGGTATCAGGAAATAC
TAACGGATCCCTCCTATAAAGGTCAACTTGTCACACTCACCTACCCAATGATTGGGAACTACGGAATCAACCCCGACGAT
ATGGAATCTGATCGTATTCAGGCATCTGGCCTCATCGTGAAAGAATATGTCAAACGACCTTCCAATTTCCAATCGAAAGA
AACACTCAGTGAATTTCTAATTCGTTTTGGTGTGCCTGCCATCGAAGGGATCGACACACGCAAACTAACACGCATCATTC
GGAACTCTGGTGCCATGAATTGTGGGATCTTTATCAGTGAAACTTACGAGGATTCATTTTTAGAAACTGTAAAGAATGCA
CCTTCAATGGAAGGCCAAGACTTAGCCCAAATTGTGACTTGCGAAAAGCCATATGTCTTCGGCGCACACTCTCCGAGTAA
ATTTAAACTTGCCGTTTATGATTTTGGTATCAAAAGAAATATCTTACGTTTACTTGATTCCGCTGGTTTTAATGTTCATG
TTTTCCCGGCTAAAACAAAAGCAGAAGACTTACTCAAAGAAGGATTTGATGCTTTCTTTTTATCCAATGGTCCAGGTGAC
CCAGCTCCACTCGATTATGCAATCCAATCAGCCAAAACCATCATGGATGCAAAAAAGCCACTCTTTGGAATTTGTTTAGG
TCACCAAATCATAGGACTTGCGCTCGGAAAAAAGACCGCCAAGCTTAAGTTTGGTCATCGAGGTGGGAATCATCCAGTAA
GAAATGAAGAAACTGGTAAAATTGAAATCACTTCTCAAAACCACGGTTTTCATGTTCTCGGCGAGTCTTCGGAAGATATG
CCAATCACACGTCTCAATCTTTTTGACAATACGGTAGCTGGACTCAAAACAAAAGGATTACCTGTGATGGCAGTCCAATA
CCACCCGGAAGCTTGTCCGGGTCCTCATGACTCAGCCTATCATTTCCAAGAATTTTATACTATGGTAGAAACCTCAAAAG
GATAA

Upstream 100 bases:

>100_bases
GAAATGGCAGAAGCCCTTTCCCAAATGAAAACAGTCACAATTGACGGGGAATACCGTTATGACCGGATCAAACGTTATTA
TGTAAAAGATTGATGAATTT

Downstream 100 bases:

>100_bases
ACTTTGGTAAACCGAAGGGGGTTATATGTCATACAAAGAAGATAAAAATTTCTGGGGAATACCGTACGGAACTGAAATTT
CTGCGGAAGCGTTCGTTAAA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MFASLAQFLGMDLMQAFLVLANGTVMKGRSFGANKNSIGEVVFNTSMAGYQEILTDPSYKGQLVTLTYPMIGNYGINPDD
MESDRIQASGLIVKEYVKRPSNFQSKETLSEFLIRFGVPAIEGIDTRKLTRIIRNSGAMNCGIFISETYEDSFLETVKNA
PSMEGQDLAQIVTCEKPYVFGAHSPSKFKLAVYDFGIKRNILRLLDSAGFNVHVFPAKTKAEDLLKEGFDAFFLSNGPGD
PAPLDYAIQSAKTIMDAKKPLFGICLGHQIIGLALGKKTAKLKFGHRGGNHPVRNEETGKIEITSQNHGFHVLGESSEDM
PITRLNLFDNTVAGLKTKGLPVMAVQYHPEACPGPHDSAYHFQEFYTMVETSKG

Sequences:

>Translated_374_residues
MFASLAQFLGMDLMQAFLVLANGTVMKGRSFGANKNSIGEVVFNTSMAGYQEILTDPSYKGQLVTLTYPMIGNYGINPDD
MESDRIQASGLIVKEYVKRPSNFQSKETLSEFLIRFGVPAIEGIDTRKLTRIIRNSGAMNCGIFISETYEDSFLETVKNA
PSMEGQDLAQIVTCEKPYVFGAHSPSKFKLAVYDFGIKRNILRLLDSAGFNVHVFPAKTKAEDLLKEGFDAFFLSNGPGD
PAPLDYAIQSAKTIMDAKKPLFGICLGHQIIGLALGKKTAKLKFGHRGGNHPVRNEETGKIEITSQNHGFHVLGESSEDM
PITRLNLFDNTVAGLKTKGLPVMAVQYHPEACPGPHDSAYHFQEFYTMVETSKG
>Mature_374_residues
MFASLAQFLGMDLMQAFLVLANGTVMKGRSFGANKNSIGEVVFNTSMAGYQEILTDPSYKGQLVTLTYPMIGNYGINPDD
MESDRIQASGLIVKEYVKRPSNFQSKETLSEFLIRFGVPAIEGIDTRKLTRIIRNSGAMNCGIFISETYEDSFLETVKNA
PSMEGQDLAQIVTCEKPYVFGAHSPSKFKLAVYDFGIKRNILRLLDSAGFNVHVFPAKTKAEDLLKEGFDAFFLSNGPGD
PAPLDYAIQSAKTIMDAKKPLFGICLGHQIIGLALGKKTAKLKFGHRGGNHPVRNEETGKIEITSQNHGFHVLGESSEDM
PITRLNLFDNTVAGLKTKGLPVMAVQYHPEACPGPHDSAYHFQEFYTMVETSKG

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=372, Percent_Identity=37.6344086021505, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI21361331, Length=381, Percent_Identity=37.7952755905512, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI169790915, Length=381, Percent_Identity=37.7952755905512, Blast_Score=235, Evalue=4e-62,
Organism=Escherichia coli, GI1786215, Length=374, Percent_Identity=46.524064171123, Blast_Score=335, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI193204318, Length=379, Percent_Identity=40.8970976253298, Blast_Score=249, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=395, Percent_Identity=36.4556962025316, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324878, Length=373, Percent_Identity=37.2654155495979, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI45555749, Length=380, Percent_Identity=37.6315789473684, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24642586, Length=380, Percent_Identity=37.6315789473684, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41131; Mature: 41131

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFASLAQFLGMDLMQAFLVLANGTVMKGRSFGANKNSIGEVVFNTSMAGYQEILTDPSYK
CHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCC
GQLVTLTYPMIGNYGINPDDMESDRIQASGLIVKEYVKRPSNFQSKETLSEFLIRFGVPA
CEEEEEEECCCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCH
IEGIDTRKLTRIIRNSGAMNCGIFISETYEDSFLETVKNAPSMEGQDLAQIVTCEKPYVF
HCCCCHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEE
GAHSPSKFKLAVYDFGIKRNILRLLDSAGFNVHVFPAKTKAEDLLKEGFDAFFLSNGPGD
ECCCCCCEEEEEEECCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCC
PAPLDYAIQSAKTIMDAKKPLFGICLGHQIIGLALGKKTAKLKFGHRGGNHPVRNEETGK
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCE
IEITSQNHGFHVLGESSEDMPITRLNLFDNTVAGLKTKGLPVMAVQYHPEACPGPHDSAY
EEEEECCCCEEEECCCCCCCCEEEEEHHHCHHHCCCCCCCEEEEEEECCCCCCCCCCCHH
HFQEFYTMVETSKG
HHHHHHHHHHCCCC
>Mature Secondary Structure
MFASLAQFLGMDLMQAFLVLANGTVMKGRSFGANKNSIGEVVFNTSMAGYQEILTDPSYK
CHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCC
GQLVTLTYPMIGNYGINPDDMESDRIQASGLIVKEYVKRPSNFQSKETLSEFLIRFGVPA
CEEEEEEECCCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCH
IEGIDTRKLTRIIRNSGAMNCGIFISETYEDSFLETVKNAPSMEGQDLAQIVTCEKPYVF
HCCCCHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEE
GAHSPSKFKLAVYDFGIKRNILRLLDSAGFNVHVFPAKTKAEDLLKEGFDAFFLSNGPGD
ECCCCCCEEEEEEECCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCC
PAPLDYAIQSAKTIMDAKKPLFGICLGHQIIGLALGKKTAKLKFGHRGGNHPVRNEETGK
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCE
IEITSQNHGFHVLGESSEDMPITRLNLFDNTVAGLKTKGLPVMAVQYHPEACPGPHDSAY
EEEEECCCCEEEECCCCCCCCEEEEEHHHCHHHCCCCCCCEEEEEEECCCCCCCCCCCHH
HFQEFYTMVETSKG
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA