Click here to switch to the map view.

The map label for this gene is ilvB1 [H]

Identifier: 183220202

GI number: 183220202

Start: 811077

End: 812798

Strand: Direct

Name: ilvB1 [H]

Synonym: LEPBI_I0792

Alternate gene names: 183220202

Gene position: 811077-812798 (Clockwise)

Preceding gene: 183220201

Following gene: 183220205

Centisome position: 22.53

GC content: 39.43

Gene sequence:

>1722_bases
ATGTGGGAAGAAATCACTGTTAGTCAATATATCATACAATTCTTAGAAGCGAAAGGAATCCAAAAGATTGCGGGGGTTCC
TGGTGGAACGATTTTGCCACTATACGAATGTTTGGCGGCATCCAATATCCATCACATTCTAGCAAGGCATGAACAAGGTG
CCGGTTTTATTGCGCAAGGTATGGCCAGAAGTTCAGGGGAAATCAGTGCTGTTTTTGTTTCCTCTGGACCTGGGGTTGCC
AACTTGATCACGGCCATTGCTGACGCACATCGAGATTCGGTTCCCGTTTTGATCTTTTCAGGACAGGTTCCATTGGCATT
AAAGGGAACAGATGCGTTCCAAGAATTGGACACTGAATCAATGGTATCCTCAATTGTGAAAAAAATTTATGCCATTGAAG
ATCCAACTCAAATCCCAATCGTTCTCAACGAAGCCTATGCACTCGCAATGGAAGGAAAAAAGGGTCCTGTTTGGATTGAT
TTGCCAAAAGACATCCAAATGAGCAGGATCTCCATTTCGATGGAGACTTTCTCAAATGAATTTTCAATTCAGAGACATTT
TTCAGTTGAGACAGAGAATTCGATCGAAGCGAACTCTCATTCAATCCATGAATTTTTAAAACAATGGAACGAGTATTTAT
GCCAAGCAAAGTTTCCTTTACTCTATATTGGTGGAGGTGCCAAAAAAGAATACAAACGTTTGCGTGAACTCGTGAATCAG
TATCAAATACCGGTTGTGACAACACTCATGGGTCTTGGGATATTTGAAAAAGAGGATCCCTTGTATTTAGGTATGATGGG
AATGCATGGAACCATTGGTGCGAATGAAGCTCTTGGCGTATGTGATTTACTCATTGCGATTGGTGTTCGTTTTGATGACC
GGGCAATTGGAAACAAAGATACATTTTGTCCGGATGCAAAAATCATTCATATCGATATCGATGCAAATGAAATTGGAAAA
ATTAGAAAACCAAATTTGAGTATCAAAAAGGATATCTCAGATTTGATTCCATTTTTACTTGAGGATCGACTTCACAATGA
ATTCGCTTACCTAAAAAAATCTAGGAATGAACCAATTTTGAACCAAATACAAACTTGGAAACAAATACCTGAATCACATC
CTGCGAAGGAGTTAATCCTTGGATTGGCAGAAAAGATTCCCAATGAAGATCATTTTATCCTGACTGATGTGGGGCAACAC
CAAATGTGGGTAGCACAGTACTATCCATTTTCAAAACCAACACAATGGATCACTTCTGGTGGCCAGGGAACGATGGGTTT
TGGATTGCCAACATCCATTGGTGTGTCTCTGTCCCATCCAACGCATCCAGTGTATTGTTTCACTGGTGATGGCTCAATTA
TGATGAATTTACAGGAGTTAGCGACACTCAGAGAACAAAATCTGAATGTAAAAATCATTCTAATGAATAATGAACACTTA
GGATTAGTGAAGCAACAACAGGATTTGTTTTATGGGAATGTTTATTCTGGATCGCAGTTTCATTTTCATCCCAACTTTTC
TATGTTATGTGCAGCGTTTGGGATTCCTTATTTGCTTTGGGAGAACGATCAAAATCAAGATCAAGATCACTTACAAACTA
TTTTAGCACAAAATGGTCCGAGCTTCGTTGAGATCAAAGTACCATCTGAATGGGGAGTGTATCCATTTGTTCCTGGTGGT
AAGTCCAATCAGGAATATATTTTAGAATTGGTAAAAACGTAG

Upstream 100 bases:

>100_bases
GTCTCCTCAACCATACTTAGCTTTCTGTCATAATCTTCTTTAGTTTCCTCGGTCGACAGAAAGCAAATGTCGACGGGGGA
ACCATCAAAGGAGACATGTT

Downstream 100 bases:

>100_bases
AGCATATCTCCCATAGAATCCAATGGATTTTATGGGAGATAAGTTGATTCTTTTAAATCGCTTTGTTTCCTCGTTCACCA
CTTCGAATGCGAATCACTTC

Product: acetolactate synthase isozyme I large subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MWEEITVSQYIIQFLEAKGIQKIAGVPGGTILPLYECLAASNIHHILARHEQGAGFIAQGMARSSGEISAVFVSSGPGVA
NLITAIADAHRDSVPVLIFSGQVPLALKGTDAFQELDTESMVSSIVKKIYAIEDPTQIPIVLNEAYALAMEGKKGPVWID
LPKDIQMSRISISMETFSNEFSIQRHFSVETENSIEANSHSIHEFLKQWNEYLCQAKFPLLYIGGGAKKEYKRLRELVNQ
YQIPVVTTLMGLGIFEKEDPLYLGMMGMHGTIGANEALGVCDLLIAIGVRFDDRAIGNKDTFCPDAKIIHIDIDANEIGK
IRKPNLSIKKDISDLIPFLLEDRLHNEFAYLKKSRNEPILNQIQTWKQIPESHPAKELILGLAEKIPNEDHFILTDVGQH
QMWVAQYYPFSKPTQWITSGGQGTMGFGLPTSIGVSLSHPTHPVYCFTGDGSIMMNLQELATLREQNLNVKIILMNNEHL
GLVKQQQDLFYGNVYSGSQFHFHPNFSMLCAAFGIPYLLWENDQNQDQDHLQTILAQNGPSFVEIKVPSEWGVYPFVPGG
KSNQEYILELVKT

Sequences:

>Translated_573_residues
MWEEITVSQYIIQFLEAKGIQKIAGVPGGTILPLYECLAASNIHHILARHEQGAGFIAQGMARSSGEISAVFVSSGPGVA
NLITAIADAHRDSVPVLIFSGQVPLALKGTDAFQELDTESMVSSIVKKIYAIEDPTQIPIVLNEAYALAMEGKKGPVWID
LPKDIQMSRISISMETFSNEFSIQRHFSVETENSIEANSHSIHEFLKQWNEYLCQAKFPLLYIGGGAKKEYKRLRELVNQ
YQIPVVTTLMGLGIFEKEDPLYLGMMGMHGTIGANEALGVCDLLIAIGVRFDDRAIGNKDTFCPDAKIIHIDIDANEIGK
IRKPNLSIKKDISDLIPFLLEDRLHNEFAYLKKSRNEPILNQIQTWKQIPESHPAKELILGLAEKIPNEDHFILTDVGQH
QMWVAQYYPFSKPTQWITSGGQGTMGFGLPTSIGVSLSHPTHPVYCFTGDGSIMMNLQELATLREQNLNVKIILMNNEHL
GLVKQQQDLFYGNVYSGSQFHFHPNFSMLCAAFGIPYLLWENDQNQDQDHLQTILAQNGPSFVEIKVPSEWGVYPFVPGG
KSNQEYILELVKT
>Mature_573_residues
MWEEITVSQYIIQFLEAKGIQKIAGVPGGTILPLYECLAASNIHHILARHEQGAGFIAQGMARSSGEISAVFVSSGPGVA
NLITAIADAHRDSVPVLIFSGQVPLALKGTDAFQELDTESMVSSIVKKIYAIEDPTQIPIVLNEAYALAMEGKKGPVWID
LPKDIQMSRISISMETFSNEFSIQRHFSVETENSIEANSHSIHEFLKQWNEYLCQAKFPLLYIGGGAKKEYKRLRELVNQ
YQIPVVTTLMGLGIFEKEDPLYLGMMGMHGTIGANEALGVCDLLIAIGVRFDDRAIGNKDTFCPDAKIIHIDIDANEIGK
IRKPNLSIKKDISDLIPFLLEDRLHNEFAYLKKSRNEPILNQIQTWKQIPESHPAKELILGLAEKIPNEDHFILTDVGQH
QMWVAQYYPFSKPTQWITSGGQGTMGFGLPTSIGVSLSHPTHPVYCFTGDGSIMMNLQELATLREQNLNVKIILMNNEHL
GLVKQQQDLFYGNVYSGSQFHFHPNFSMLCAAFGIPYLLWENDQNQDQDHLQTILAQNGPSFVEIKVPSEWGVYPFVPGG
KSNQEYILELVKT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=553, Percent_Identity=24.2314647377938, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI93004078, Length=510, Percent_Identity=24.3137254901961, Blast_Score=128, Evalue=2e-29,
Organism=Escherichia coli, GI1790104, Length=575, Percent_Identity=44.1739130434783, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI87081685, Length=585, Percent_Identity=36.4102564102564, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1786717, Length=487, Percent_Identity=29.7741273100616, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1787096, Length=558, Percent_Identity=26.8817204301075, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=23.4767025089606, Blast_Score=109, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=528, Percent_Identity=23.6742424242424, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531299, Length=527, Percent_Identity=23.9089184060721, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17531301, Length=527, Percent_Identity=23.9089184060721, Blast_Score=108, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=590, Percent_Identity=34.9152542372881, Blast_Score=338, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6320816, Length=499, Percent_Identity=24.0480961923848, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320588, Length=539, Percent_Identity=20.7792207792208, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=494, Percent_Identity=22.2672064777328, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321524, Length=502, Percent_Identity=21.3147410358566, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922626, Length=493, Percent_Identity=22.3123732251521, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63958; Mature: 63958

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWEEITVSQYIIQFLEAKGIQKIAGVPGGTILPLYECLAASNIHHILARHEQGAGFIAQG
CCCCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCHHHHH
MARSSGEISAVFVSSGPGVANLITAIADAHRDSVPVLIFSGQVPLALKGTDAFQELDTES
HCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCEEEECCHHHHHCCHHH
MVSSIVKKIYAIEDPTQIPIVLNEAYALAMEGKKGPVWIDLPKDIQMSRISISMETFSNE
HHHHHHHHHHCCCCCCCCEEEEECEEEEEECCCCCCEEEECCCCCCEEEEEEEHHHCCCC
FSIQRHFSVETENSIEANSHSIHEFLKQWNEYLCQAKFPLLYIGGGAKKEYKRLRELVNQ
EEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
YQIPVVTTLMGLGIFEKEDPLYLGMMGMHGTIGANEALGVCDLLIAIGVRFDDRAIGNKD
HCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
TFCPDAKIIHIDIDANEIGKIRKPNLSIKKDISDLIPFLLEDRLHNEFAYLKKSRNEPIL
CCCCCCEEEEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
NQIQTWKQIPESHPAKELILGLAEKIPNEDHFILTDVGQHQMWVAQYYPFSKPTQWITSG
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHCC
GQGTMGFGLPTSIGVSLSHPTHPVYCFTGDGSIMMNLQELATLREQNLNVKIILMNNEHL
CCCCCCCCCCCCCCEEECCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCC
GLVKQQQDLFYGNVYSGSQFHFHPNFSMLCAAFGIPYLLWENDQNQDQDHLQTILAQNGP
CEEECCCCCEEEEECCCCEEEECCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCC
SFVEIKVPSEWGVYPFVPGGKSNQEYILELVKT
CEEEEEECCCCCCEECCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MWEEITVSQYIIQFLEAKGIQKIAGVPGGTILPLYECLAASNIHHILARHEQGAGFIAQG
CCCCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCHHHHH
MARSSGEISAVFVSSGPGVANLITAIADAHRDSVPVLIFSGQVPLALKGTDAFQELDTES
HCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCEEEECCHHHHHCCHHH
MVSSIVKKIYAIEDPTQIPIVLNEAYALAMEGKKGPVWIDLPKDIQMSRISISMETFSNE
HHHHHHHHHHCCCCCCCCEEEEECEEEEEECCCCCCEEEECCCCCCEEEEEEEHHHCCCC
FSIQRHFSVETENSIEANSHSIHEFLKQWNEYLCQAKFPLLYIGGGAKKEYKRLRELVNQ
EEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHH
YQIPVVTTLMGLGIFEKEDPLYLGMMGMHGTIGANEALGVCDLLIAIGVRFDDRAIGNKD
HCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
TFCPDAKIIHIDIDANEIGKIRKPNLSIKKDISDLIPFLLEDRLHNEFAYLKKSRNEPIL
CCCCCCEEEEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
NQIQTWKQIPESHPAKELILGLAEKIPNEDHFILTDVGQHQMWVAQYYPFSKPTQWITSG
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHCC
GQGTMGFGLPTSIGVSLSHPTHPVYCFTGDGSIMMNLQELATLREQNLNVKIILMNNEHL
CCCCCCCCCCCCCCEEECCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCC
GLVKQQQDLFYGNVYSGSQFHFHPNFSMLCAAFGIPYLLWENDQNQDQDHLQTILAQNGP
CEEECCCCCEEEEECCCCEEEECCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCC
SFVEIKVPSEWGVYPFVPGGKSNQEYILELVKT
CEEEEEECCCCCCEECCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]