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The map label for this gene is pykF [H]

Identifier: 183220175

GI number: 183220175

Start: 780383

End: 781819

Strand: Reverse

Name: pykF [H]

Synonym: LEPBI_I0764

Alternate gene names: 183220175

Gene position: 781819-780383 (Counterclockwise)

Preceding gene: 183220179

Following gene: 183220167

Centisome position: 21.72

GC content: 41.06

Gene sequence:

>1437_bases
ATGCTGGAAGACCTAAAAATCCCCAAAAAACGCACGAAAATTATTTGTACCATTGGACCTGCTTCAGCAAATCGAGAGAC
AATTCTCAATTTAATTTATGCTGGTATGGACCTCGCTCGTATGAACTTTTCTCATTCCACTCACGAATACCACAAAGAGA
TTTTTGAATTGTTACGGGAATGTGAACAGGAATCCGGAAAATCAATTGGGATTTTGGCTGATTTACAAGGCCCCAAAATC
CGCACAGGCAAACTCGGAGCAGGTACCATCGAACTAAAAGCAGGGGACCAAATCGCCATCAATAACAAAGGAGATTTTTT
AGGGAACAGGGATGAGATTGGATGTACCTACCAATACATTTTAAATGATATCGATGTTGGGCATAAAATTCTCATCGACG
ATGGAAAACTGGCCTTTGTTGTAAAATCCAAATCGAAAGAAAAAGCAATTTTAGAAACTGTCATTGGTGGGGTTTTGAAA
GACAATAAAGGGATTAACTTACCAGGGACTCCCATATCTGCCCCAGCACTTTCTGAAAAAGACATTGAAGACTTACAATT
TGCACTCTCTCTTGGAGTGGATTACATTGCCCTATCATTTGTTAGACGAGCAAATGATTTAGAGATGGCACGTCAATTTA
TGAAAGATAGTTATGCAGGCCTTATCGCCAAAATAGAACGACCTGAAGCCATCCAAAATATCGAAGAAATCATTGATCAC
TGCGATGGAATCATGATCGCGAGAGGTGATTTAGGTGTGGAACTTGATACACAGTATGTACCGATCATCCAAAAAGAAAT
GATCACAAAACTAAACCAAAGAGGAAAACCAGTCATCACCGCCACACAGATGTTAGAGACAATGATTGACAACCCTCGTC
CCACAAGGGCCGAGGCAAGTGATGTGGCAAATGCAGTAATGGATGGAACTGATGCGGTGATGTTATCTGGAGAAACTGCA
TCTGGTAAATACCCAGTGGAAACTGTGAAAACAATGACGAGCATCATCCAAGCGGCAGAAGAATCGGAAATTTATTTATC
TCATTTACGTAGTATGAACCGCACTGAATTTGAAGTGGAACGAACGGCTCTCGGTAGTGCTGCTGAATCGATCTCCAGAT
CCATTCATGCAAAGGCAATCATCAACTTCACAAGGTCAGGTTATTCGTCCTTACTTTCATCGGAATTTCGTCCGCTCAAT
CCTATTTATTCGTTCACTCCTTTTTTGGGAACTGCAAGGAAGATGCAATTGTTTTGGGGAGTGGAATCCTATGTGATGCC
GATGATGGATAAGTTTCCAGACATGATCGCATTTATGAGTAAAACGTTAAAATCGGAAGGGAAATTAAAATCGGGAGATA
CGGTTGTGATTCTGTCAGGTGCACCTGGATCAGTGGCGCAAACGGTAGATTTCATACAGATTCACAAAATCAAATAA

Upstream 100 bases:

>100_bases
TGATGCCATTAAATTGATATTTTTGCGAAAAATGACACAAGTCAATTCAATCCATACAAATTACTTGCAGGAAAAAACAA
AAAAGCCAAGTTTAGGGTCT

Downstream 100 bases:

>100_bases
CAAGTGTTTTACGAATTCCTTGGGCGGCAATCACACTTGTCGTCCAAGCGTTTTGAAAATTAAAACCACCGGTAATCCCA
TCCACATCGATCACCTCACC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLEDLKIPKKRTKIICTIGPASANRETILNLIYAGMDLARMNFSHSTHEYHKEIFELLRECEQESGKSIGILADLQGPKI
RTGKLGAGTIELKAGDQIAINNKGDFLGNRDEIGCTYQYILNDIDVGHKILIDDGKLAFVVKSKSKEKAILETVIGGVLK
DNKGINLPGTPISAPALSEKDIEDLQFALSLGVDYIALSFVRRANDLEMARQFMKDSYAGLIAKIERPEAIQNIEEIIDH
CDGIMIARGDLGVELDTQYVPIIQKEMITKLNQRGKPVITATQMLETMIDNPRPTRAEASDVANAVMDGTDAVMLSGETA
SGKYPVETVKTMTSIIQAAEESEIYLSHLRSMNRTEFEVERTALGSAAESISRSIHAKAIINFTRSGYSSLLSSEFRPLN
PIYSFTPFLGTARKMQLFWGVESYVMPMMDKFPDMIAFMSKTLKSEGKLKSGDTVVILSGAPGSVAQTVDFIQIHKIK

Sequences:

>Translated_478_residues
MLEDLKIPKKRTKIICTIGPASANRETILNLIYAGMDLARMNFSHSTHEYHKEIFELLRECEQESGKSIGILADLQGPKI
RTGKLGAGTIELKAGDQIAINNKGDFLGNRDEIGCTYQYILNDIDVGHKILIDDGKLAFVVKSKSKEKAILETVIGGVLK
DNKGINLPGTPISAPALSEKDIEDLQFALSLGVDYIALSFVRRANDLEMARQFMKDSYAGLIAKIERPEAIQNIEEIIDH
CDGIMIARGDLGVELDTQYVPIIQKEMITKLNQRGKPVITATQMLETMIDNPRPTRAEASDVANAVMDGTDAVMLSGETA
SGKYPVETVKTMTSIIQAAEESEIYLSHLRSMNRTEFEVERTALGSAAESISRSIHAKAIINFTRSGYSSLLSSEFRPLN
PIYSFTPFLGTARKMQLFWGVESYVMPMMDKFPDMIAFMSKTLKSEGKLKSGDTVVILSGAPGSVAQTVDFIQIHKIK
>Mature_478_residues
MLEDLKIPKKRTKIICTIGPASANRETILNLIYAGMDLARMNFSHSTHEYHKEIFELLRECEQESGKSIGILADLQGPKI
RTGKLGAGTIELKAGDQIAINNKGDFLGNRDEIGCTYQYILNDIDVGHKILIDDGKLAFVVKSKSKEKAILETVIGGVLK
DNKGINLPGTPISAPALSEKDIEDLQFALSLGVDYIALSFVRRANDLEMARQFMKDSYAGLIAKIERPEAIQNIEEIIDH
CDGIMIARGDLGVELDTQYVPIIQKEMITKLNQRGKPVITATQMLETMIDNPRPTRAEASDVANAVMDGTDAVMLSGETA
SGKYPVETVKTMTSIIQAAEESEIYLSHLRSMNRTEFEVERTALGSAAESISRSIHAKAIINFTRSGYSSLLSSEFRPLN
PIYSFTPFLGTARKMQLFWGVESYVMPMMDKFPDMIAFMSKTLKSEGKLKSGDTVVILSGAPGSVAQTVDFIQIHKIK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=486, Percent_Identity=40.5349794238683, Blast_Score=331, Evalue=7e-91,
Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=40.1234567901235, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=40.1234567901235, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI10835121, Length=487, Percent_Identity=39.4250513347023, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI32967597, Length=487, Percent_Identity=39.4250513347023, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=40.6896551724138, Blast_Score=209, Evalue=5e-54,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=40.6896551724138, Blast_Score=209, Evalue=5e-54,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=38.4937238493724, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=38.4937238493724, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=36.7441860465116, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1787965, Length=464, Percent_Identity=39.4396551724138, Blast_Score=314, Evalue=9e-87,
Organism=Escherichia coli, GI1788160, Length=464, Percent_Identity=39.2241379310345, Blast_Score=295, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI17544584, Length=467, Percent_Identity=37.4732334047109, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI17506829, Length=469, Percent_Identity=37.5266524520256, Blast_Score=295, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI71984413, Length=469, Percent_Identity=37.5266524520256, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI71984406, Length=469, Percent_Identity=37.5266524520256, Blast_Score=294, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI17506831, Length=469, Percent_Identity=37.5266524520256, Blast_Score=294, Evalue=9e-80,
Organism=Saccharomyces cerevisiae, GI6319279, Length=466, Percent_Identity=39.2703862660944, Blast_Score=290, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=36.5503080082135, Blast_Score=266, Evalue=4e-72,
Organism=Drosophila melanogaster, GI24648964, Length=482, Percent_Identity=39.4190871369295, Blast_Score=327, Evalue=8e-90,
Organism=Drosophila melanogaster, GI28571814, Length=482, Percent_Identity=39.4190871369295, Blast_Score=327, Evalue=9e-90,
Organism=Drosophila melanogaster, GI24648966, Length=407, Percent_Identity=41.031941031941, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24581235, Length=471, Percent_Identity=35.031847133758, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24646914, Length=270, Percent_Identity=40.3703703703704, Blast_Score=176, Evalue=3e-44,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52693; Mature: 52693

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00110 PYRUVATE_KINASE ; PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEDLKIPKKRTKIICTIGPASANRETILNLIYAGMDLARMNFSHSTHEYHKEIFELLRE
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHH
CEQESGKSIGILADLQGPKIRTGKLGAGTIELKAGDQIAINNKGDFLGNRDEIGCTYQYI
HHHHCCCEEEEEEECCCCCEECCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCHHHHHH
LNDIDVGHKILIDDGKLAFVVKSKSKEKAILETVIGGVLKDNKGINLPGTPISAPALSEK
HHHCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DIEDLQFALSLGVDYIALSFVRRANDLEMARQFMKDSYAGLIAKIERPEAIQNIEEIIDH
CHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CDGIMIARGDLGVELDTQYVPIIQKEMITKLNQRGKPVITATQMLETMIDNPRPTRAEAS
CCCEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCHHH
DVANAVMDGTDAVMLSGETASGKYPVETVKTMTSIIQAAEESEIYLSHLRSMNRTEFEVE
HHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RTALGSAAESISRSIHAKAIINFTRSGYSSLLSSEFRPLNPIYSFTPFLGTARKMQLFWG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHC
VESYVMPMMDKFPDMIAFMSKTLKSEGKLKSGDTVVILSGAPGSVAQTVDFIQIHKIK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHEEEECC
>Mature Secondary Structure
MLEDLKIPKKRTKIICTIGPASANRETILNLIYAGMDLARMNFSHSTHEYHKEIFELLRE
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHH
CEQESGKSIGILADLQGPKIRTGKLGAGTIELKAGDQIAINNKGDFLGNRDEIGCTYQYI
HHHHCCCEEEEEEECCCCCEECCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCHHHHHH
LNDIDVGHKILIDDGKLAFVVKSKSKEKAILETVIGGVLKDNKGINLPGTPISAPALSEK
HHHCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DIEDLQFALSLGVDYIALSFVRRANDLEMARQFMKDSYAGLIAKIERPEAIQNIEEIIDH
CHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CDGIMIARGDLGVELDTQYVPIIQKEMITKLNQRGKPVITATQMLETMIDNPRPTRAEAS
CCCEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCHHH
DVANAVMDGTDAVMLSGETASGKYPVETVKTMTSIIQAAEESEIYLSHLRSMNRTEFEVE
HHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RTALGSAAESISRSIHAKAIINFTRSGYSSLLSSEFRPLNPIYSFTPFLGTARKMQLFWG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHC
VESYVMPMMDKFPDMIAFMSKTLKSEGKLKSGDTVVILSGAPGSVAQTVDFIQIHKIK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA