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The map label for this gene is gloA [H]

Identifier: 183220173

GI number: 183220173

Start: 778720

End: 779142

Strand: Direct

Name: gloA [H]

Synonym: LEPBI_I0762

Alternate gene names: 183220173

Gene position: 778720-779142 (Clockwise)

Preceding gene: 183220172

Following gene: 183220174

Centisome position: 21.63

GC content: 40.19

Gene sequence:

>423_bases
ATGAAATATTTACACACAATGATTCGTGTTCAAAATTTGGAGAAAGCCCTCCATTTTTTTGTCGATGTTTTGGGATTACA
AGTTTCTCGGAAGAAAGATTACCCAGAAGGTAAATTTACCCTCGTATTTTTGTCTACAGGGGAAGAGAATGCTTCCGAAA
TCGAACTCACATATAACTGGGATCAGGCGGAAGCATATACTGTTGGGCGAAATTTTGGACATTTGGCATACGAAGTAGAT
GATATTTACGATTTTTGCGAAAAAATCCAATCCATGGGAGTAGTGATCAGTAGGCCACCTAGGGATGGTCGCATGGCATT
TGTTAGGTCACCAGATTCTATTTCCATCGAATTATTACAAAAAGGGAATCCTTTGCCACCGAAAGAACCTTGGGTTTCCA
TGCCAAATACTGGGGAGTGGTAA

Upstream 100 bases:

>100_bases
ACTCGAAGGGAAAAAAGAATGGCTTTCGGTCGAAATTCCTAAGTATTTCTCTTGGCTATTTCTCTTGGTGTATTTTTTTA
CATAATAGAGCCATTCACTA

Downstream 100 bases:

>100_bases
GTTTTGGGGAAAAAACCCAAAATTGCGGTGATAGGAGCGGGTGCTTCGGGGTGTTTTGCGGCTTTGCAGATTTATGAATC
ACTTGCGGGGAAGGCATCCA

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MKYLHTMIRVQNLEKALHFFVDVLGLQVSRKKDYPEGKFTLVFLSTGEENASEIELTYNWDQAEAYTVGRNFGHLAYEVD
DIYDFCEKIQSMGVVISRPPRDGRMAFVRSPDSISIELLQKGNPLPPKEPWVSMPNTGEW

Sequences:

>Translated_140_residues
MKYLHTMIRVQNLEKALHFFVDVLGLQVSRKKDYPEGKFTLVFLSTGEENASEIELTYNWDQAEAYTVGRNFGHLAYEVD
DIYDFCEKIQSMGVVISRPPRDGRMAFVRSPDSISIELLQKGNPLPPKEPWVSMPNTGEW
>Mature_140_residues
MKYLHTMIRVQNLEKALHFFVDVLGLQVSRKKDYPEGKFTLVFLSTGEENASEIELTYNWDQAEAYTVGRNFGHLAYEVD
DIYDFCEKIQSMGVVISRPPRDGRMAFVRSPDSISIELLQKGNPLPPKEPWVSMPNTGEW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=143, Percent_Identity=33.5664335664336, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1787940, Length=127, Percent_Identity=39.3700787401575, Blast_Score=96, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=34.5070422535211, Blast_Score=72, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20129743, Length=146, Percent_Identity=36.3013698630137, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16116; Mature: 16116

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLHTMIRVQNLEKALHFFVDVLGLQVSRKKDYPEGKFTLVFLSTGEENASEIELTYNW
CHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEEEECCCCCCEEEEEEEEC
DQAEAYTVGRNFGHLAYEVDDIYDFCEKIQSMGVVISRPPRDGRMAFVRSPDSISIELLQ
CCCEEEEECCCCCCEEEEHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCEEEEEEE
KGNPLPPKEPWVSMPNTGEW
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKYLHTMIRVQNLEKALHFFVDVLGLQVSRKKDYPEGKFTLVFLSTGEENASEIELTYNW
CHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEEEECCCCCCEEEEEEEEC
DQAEAYTVGRNFGHLAYEVDDIYDFCEKIQSMGVVISRPPRDGRMAFVRSPDSISIELLQ
CCCEEEEECCCCCCEEEEHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCEEEEEEE
KGNPLPPKEPWVSMPNTGEW
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]