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The map label for this gene is ntrB [H]

Identifier: 183220060

GI number: 183220060

Start: 651483

End: 653669

Strand: Reverse

Name: ntrB [H]

Synonym: LEPBI_I0647

Alternate gene names: 183220060

Gene position: 653669-651483 (Counterclockwise)

Preceding gene: 183220061

Following gene: 183220047

Centisome position: 18.16

GC content: 35.89

Gene sequence:

>2187_bases
TTGCTTCGCCTTGTTGTAAGTCTCGTCTGTTTTCCCTTTCTGTTATGGGCAGAAGGGGAACAAAAATTAGTCCATTTAGG
AACCAATCCCAATGCATCGCTAGAAGGAAGTTCACCTTCGTTATCCATTTATTACTTTGGATTTACAAAAGAAAGCCACT
ACTATCAAATCAAACTAGAAGAAGATCCAAATCGATATTTTCATTTTGAAAATGGAATGTTATCGGAACTCGATTGTGAA
TTGATCCAAGATGGTAAGATCACCAAACAATTCCAAACAGGGCTTCTGAGAAAAAAAAATCCAGACGTTTCCTATACAGG
TGGTTTTTTATTTCCCGCAAAAGAAAAGGGAATTTATCGTTTTCGAATCCAGTCGGATGACACACATCGAATCAATTTTA
GAATTCGAAAAGAATCAGAATTATTCCAATACACCAAATCTTTGTCCTTATGGCAAGGTCTCTATTTGGGACTTTGTATT
CTCTTGTGTTTGTTTACTGCAGCTCAATACATACTTCTGCGAGAAAAAATTTCACTTCTATTAACCTTTGCAACCTTCAC
TGTTTTATTTACTAACGTTTTACGTTCAGGTCTTTTTTATGAATATGGCTTTAGTGATTTTGATTGGTTTTATCGATATG
TACCAGGACTACTCTCACTTAGCCCAATCGGATTTGTATTATTCCTAAGGGAATTTTTACATACCAAAACAAAACACCCC
AACTTTGATCGTTATGTAATGGTATACATTTATATGATGTTTCTTTCGATGGGTGTCTCGATCTTCGACTTACAACTCTA
TTTCCGATTTATCTATACCAATAGTTTGATGTTATCAACCGCTACATTTGCTTATTCGATCTATTGTTTAATTCAAAAAA
AGGAACATGCAAATGTATTGTTTTTTGCTTTTTTGGTTAGGCAAATTAGTACGACCTTACTCATCTTCACAAACACGGGT
TACCTGCCATCCTTTCCTTTCCTAAGTTCAGCCAATGAAATTGGTGCCGCAATCCAGATGACAATCTTTACAATTGTTAT
CTCAAAATTTCAGATCCAAGAAAGGATCATCAAAGAACAAACGGTAACAAAAGTAAACGAAGAATTGGAATTTATGGTTT
CAGAAAGAACCAAAGAATTACAGTTACAAAAAGAAAAATTAGAAAATGCCATTTTACAATTAAGCCAAACAGAAAATCAA
TTGGCACTTTCGGAAAAGATGACTGAGCTCGGTAAACTCGTTGCAGGAGTTGCACATGAAATCAATAACCCACTCAGTGC
AATCAAAGCATCGATTGAAACTTTAATGGAATCGAAACAAAATGAAACCATTCACTTAGGCACAAAAGAAAATATCTACT
CAACACTAGACATTGCTCAAATCCAAACACTTACTCGTATGTTCCAATACCAATCCGACTTTGGGCTTGTGGCAAGTTAT
ACAGAGCGAAAAGAGAAAAAAGCAGAACTCAAAAAAATTCTAAAAGACAATGGTTTGGATTATGAAGAAGCAACCTTAGA
AAAATTTCTAGATGTTGGGATCACGAAATTAGAAGAGAATCAAATCCATTTATTACAACAAGGGAAAGACAAACTCACTG
ATTTGATTTTAGAAGAAAAAAACTTTCGCCTTCACTTATCCATCATTCAAATTGCCGTGGATCGTTCGTCCAAAATCATC
CTCGCACTCAAAAACTTTTCAAGAGTCACACATTCAGAACAAAGGAAAATTTTCACCTTACTCGATAACATTGAAACTGT
GATTACGATCTATCAGTACAGAATGCGAAGTAAAGTTTCCTTAAAAAAAACATTTTTAACTGATGCTACAATCCTTGGTT
GGCCAGAAGACCTAATGCGTGTTTGGACAAACCTCATTCTCAATGCATTGGAAGCGATGAAAACGAAAGGGAATTTAACC
ATCAGTACAGAAAAAAATGGAAGGCACGTAGAAGTAAAGGTCATTGACAATGGGCCTGGAATCCCGATTGAAATTCAAAA
TAGAATATTTGAACCATTTTTTACGACCAAACAGCAAGGGGAAGGTACGGGTATGGGACTTGGAATCACGAAGTCCATCA
TCGAAAAACATTTTGGGAAAATCACAGTGGAATCAGAACCAGGAAGGACTTGTTTTTGTATTCTATTACCTGCGATCGAA
CTCATCGACCCTCACGCCACGGAATGA

Upstream 100 bases:

>100_bases
AATACTACCAGTGATGCAAAATCAGTGATCATTCGTGGAACATTGAAATGTAATGCTCCGAATATTGTCCCACAATAAAT
AATCGATTGTTTCCTTAGAT

Downstream 100 bases:

>100_bases
ATCATTTTTCTTAAAAAACCAAACTCAAGTAAGACGACTCAGTTGTTCTATAAAGAGTTCTCGTTCCTCTGATTTAGGAA
ACTGATTGAGGAATCGTAAT

Product: putative two-component sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 728; Mature: 728

Protein sequence:

>728_residues
MLRLVVSLVCFPFLLWAEGEQKLVHLGTNPNASLEGSSPSLSIYYFGFTKESHYYQIKLEEDPNRYFHFENGMLSELDCE
LIQDGKITKQFQTGLLRKKNPDVSYTGGFLFPAKEKGIYRFRIQSDDTHRINFRIRKESELFQYTKSLSLWQGLYLGLCI
LLCLFTAAQYILLREKISLLLTFATFTVLFTNVLRSGLFYEYGFSDFDWFYRYVPGLLSLSPIGFVLFLREFLHTKTKHP
NFDRYVMVYIYMMFLSMGVSIFDLQLYFRFIYTNSLMLSTATFAYSIYCLIQKKEHANVLFFAFLVRQISTTLLIFTNTG
YLPSFPFLSSANEIGAAIQMTIFTIVISKFQIQERIIKEQTVTKVNEELEFMVSERTKELQLQKEKLENAILQLSQTENQ
LALSEKMTELGKLVAGVAHEINNPLSAIKASIETLMESKQNETIHLGTKENIYSTLDIAQIQTLTRMFQYQSDFGLVASY
TERKEKKAELKKILKDNGLDYEEATLEKFLDVGITKLEENQIHLLQQGKDKLTDLILEEKNFRLHLSIIQIAVDRSSKII
LALKNFSRVTHSEQRKIFTLLDNIETVITIYQYRMRSKVSLKKTFLTDATILGWPEDLMRVWTNLILNALEAMKTKGNLT
ISTEKNGRHVEVKVIDNGPGIPIEIQNRIFEPFFTTKQQGEGTGMGLGITKSIIEKHFGKITVESEPGRTCFCILLPAIE
LIDPHATE

Sequences:

>Translated_728_residues
MLRLVVSLVCFPFLLWAEGEQKLVHLGTNPNASLEGSSPSLSIYYFGFTKESHYYQIKLEEDPNRYFHFENGMLSELDCE
LIQDGKITKQFQTGLLRKKNPDVSYTGGFLFPAKEKGIYRFRIQSDDTHRINFRIRKESELFQYTKSLSLWQGLYLGLCI
LLCLFTAAQYILLREKISLLLTFATFTVLFTNVLRSGLFYEYGFSDFDWFYRYVPGLLSLSPIGFVLFLREFLHTKTKHP
NFDRYVMVYIYMMFLSMGVSIFDLQLYFRFIYTNSLMLSTATFAYSIYCLIQKKEHANVLFFAFLVRQISTTLLIFTNTG
YLPSFPFLSSANEIGAAIQMTIFTIVISKFQIQERIIKEQTVTKVNEELEFMVSERTKELQLQKEKLENAILQLSQTENQ
LALSEKMTELGKLVAGVAHEINNPLSAIKASIETLMESKQNETIHLGTKENIYSTLDIAQIQTLTRMFQYQSDFGLVASY
TERKEKKAELKKILKDNGLDYEEATLEKFLDVGITKLEENQIHLLQQGKDKLTDLILEEKNFRLHLSIIQIAVDRSSKII
LALKNFSRVTHSEQRKIFTLLDNIETVITIYQYRMRSKVSLKKTFLTDATILGWPEDLMRVWTNLILNALEAMKTKGNLT
ISTEKNGRHVEVKVIDNGPGIPIEIQNRIFEPFFTTKQQGEGTGMGLGITKSIIEKHFGKITVESEPGRTCFCILLPAIE
LIDPHATE
>Mature_728_residues
MLRLVVSLVCFPFLLWAEGEQKLVHLGTNPNASLEGSSPSLSIYYFGFTKESHYYQIKLEEDPNRYFHFENGMLSELDCE
LIQDGKITKQFQTGLLRKKNPDVSYTGGFLFPAKEKGIYRFRIQSDDTHRINFRIRKESELFQYTKSLSLWQGLYLGLCI
LLCLFTAAQYILLREKISLLLTFATFTVLFTNVLRSGLFYEYGFSDFDWFYRYVPGLLSLSPIGFVLFLREFLHTKTKHP
NFDRYVMVYIYMMFLSMGVSIFDLQLYFRFIYTNSLMLSTATFAYSIYCLIQKKEHANVLFFAFLVRQISTTLLIFTNTG
YLPSFPFLSSANEIGAAIQMTIFTIVISKFQIQERIIKEQTVTKVNEELEFMVSERTKELQLQKEKLENAILQLSQTENQ
LALSEKMTELGKLVAGVAHEINNPLSAIKASIETLMESKQNETIHLGTKENIYSTLDIAQIQTLTRMFQYQSDFGLVASY
TERKEKKAELKKILKDNGLDYEEATLEKFLDVGITKLEENQIHLLQQGKDKLTDLILEEKNFRLHLSIIQIAVDRSSKII
LALKNFSRVTHSEQRKIFTLLDNIETVITIYQYRMRSKVSLKKTFLTDATILGWPEDLMRVWTNLILNALEAMKTKGNLT
ISTEKNGRHVEVKVIDNGPGIPIEIQNRIFEPFFTTKQQGEGTGMGLGITKSIIEKHFGKITVESEPGRTCFCILLPAIE
LIDPHATE

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=187, Percent_Identity=29.9465240641711, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1790436, Length=114, Percent_Identity=38.5964912280702, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1790300, Length=145, Percent_Identity=31.7241379310345, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87082128, Length=140, Percent_Identity=32.8571428571429, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI145693157, Length=172, Percent_Identity=30.8139534883721, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1786783, Length=152, Percent_Identity=30.2631578947368, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1789149, Length=182, Percent_Identity=28.021978021978, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 83960; Mature: 83960

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLVVSLVCFPFLLWAEGEQKLVHLGTNPNASLEGSSPSLSIYYFGFTKESHYYQIKLE
CHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEECCCEEEEEEEE
EDPNRYFHFENGMLSELDCELIQDGKITKQFQTGLLRKKNPDVSYTGGFLFPAKEKGIYR
CCCCCEEEECCCCCCCCCHHHHCCCCHHHHHHHCCCCCCCCCCEECCCEEEECCCCCEEE
FRIQSDDTHRINFRIRKESELFQYTKSLSLWQGLYLGLCILLCLFTAAQYILLREKISLL
EEECCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTFATFTVLFTNVLRSGLFYEYGFSDFDWFYRYVPGLLSLSPIGFVLFLREFLHTKTKHP
HHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
NFDRYVMVYIYMMFLSMGVSIFDLQLYFRFIYTNSLMLSTATFAYSIYCLIQKKEHANVL
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEEEEECCCCCHH
FFAFLVRQISTTLLIFTNTGYLPSFPFLSSANEIGAAIQMTIFTIVISKFQIQERIIKEQ
HHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVTKVNEELEFMVSERTKELQLQKEKLENAILQLSQTENQLALSEKMTELGKLVAGVAHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
INNPLSAIKASIETLMESKQNETIHLGTKENIYSTLDIAQIQTLTRMFQYQSDFGLVASY
HCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TERKEKKAELKKILKDNGLDYEEATLEKFLDVGITKLEENQIHLLQQGKDKLTDLILEEK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHCCCCHHHHHHCCHHHHHHHHHCCC
NFRLHLSIIQIAVDRSSKIILALKNFSRVTHSEQRKIFTLLDNIETVITIYQYRMRSKVS
CCEEEEEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKTFLTDATILGWPEDLMRVWTNLILNALEAMKTKGNLTISTEKNGRHVEVKVIDNGPG
HHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCC
IPIEIQNRIFEPFFTTKQQGEGTGMGLGITKSIIEKHFGKITVESEPGRTCFCILLPAIE
CCEEEHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCEEEEEEHHHHH
LIDPHATE
HCCCCCCC
>Mature Secondary Structure
MLRLVVSLVCFPFLLWAEGEQKLVHLGTNPNASLEGSSPSLSIYYFGFTKESHYYQIKLE
CHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEECCCEEEEEEEE
EDPNRYFHFENGMLSELDCELIQDGKITKQFQTGLLRKKNPDVSYTGGFLFPAKEKGIYR
CCCCCEEEECCCCCCCCCHHHHCCCCHHHHHHHCCCCCCCCCCEECCCEEEECCCCCEEE
FRIQSDDTHRINFRIRKESELFQYTKSLSLWQGLYLGLCILLCLFTAAQYILLREKISLL
EEECCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTFATFTVLFTNVLRSGLFYEYGFSDFDWFYRYVPGLLSLSPIGFVLFLREFLHTKTKHP
HHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
NFDRYVMVYIYMMFLSMGVSIFDLQLYFRFIYTNSLMLSTATFAYSIYCLIQKKEHANVL
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEEEEECCCCCHH
FFAFLVRQISTTLLIFTNTGYLPSFPFLSSANEIGAAIQMTIFTIVISKFQIQERIIKEQ
HHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVTKVNEELEFMVSERTKELQLQKEKLENAILQLSQTENQLALSEKMTELGKLVAGVAHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
INNPLSAIKASIETLMESKQNETIHLGTKENIYSTLDIAQIQTLTRMFQYQSDFGLVASY
HCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TERKEKKAELKKILKDNGLDYEEATLEKFLDVGITKLEENQIHLLQQGKDKLTDLILEEK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHCCCCHHHHHHCCHHHHHHHHHCCC
NFRLHLSIIQIAVDRSSKIILALKNFSRVTHSEQRKIFTLLDNIETVITIYQYRMRSKVS
CCEEEEEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKTFLTDATILGWPEDLMRVWTNLILNALEAMKTKGNLTISTEKNGRHVEVKVIDNGPG
HHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCC
IPIEIQNRIFEPFFTTKQQGEGTGMGLGITKSIIEKHFGKITVESEPGRTCFCILLPAIE
CCEEEHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCEEEEEEHHHHH
LIDPHATE
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]