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The map label for this gene is thiD [H]

Identifier: 183219985

GI number: 183219985

Start: 567566

End: 568366

Strand: Reverse

Name: thiD [H]

Synonym: LEPBI_I0567

Alternate gene names: 183219985

Gene position: 568366-567566 (Counterclockwise)

Preceding gene: 183219986

Following gene: 183219983

Centisome position: 15.79

GC content: 39.95

Gene sequence:

>801_bases
ATGAAAAAAGATTTTCCAATCACTCTGACAATCGCAGGTTCTGATTCTGGTGGTGGAGCTGGTGTCCAAGCCGATCTCAA
AACGTTTTCGTCTCTTGCGACATTTGGAACCACAGTGTTTACATGTCTCACAGCTCAAAATCCTGATGGAGTCACAGGTA
TTTATGAAATCTCTCCTGACTTTGTTTCCTCACAACTCCAAGCAGTTTCCAGTTATTTCCCGATCAAATCTGCAAAAACA
GGAATGTTATATTCGAAGGACATCATCAAATCGGTCGCTTCATTTTTTTATGATAATCCTGACATACAGTTGGTTCTCGA
TCCAGTGATGGTTGCAACAAGTGGTGCCAAACTCCTCAAAGATGATGCGATCCAATCCTTAATTGAAGATCTCATTCCAC
TTGCAAAAATAATCACACCTAACTTAGATGAGGCGTCGCTTCTTTTAGGAGAAAATATCCACCAATATGACCAGTTGGTT
CCAATGGCAAAAAAACTATTCGAAAAATACAATGTCCCCATTTTATTGAAAGGGGGACACCTACCCAATGCATCAGTAGC
GACCGATGTTTTGTTTGATGGTATATCAATATTCGAATACTCAAAACCATATCTGAAAGAAAAACATACTCATGGAACAG
GTTGTACGTATTCGGCAGCAATTACTGCCTTTTTAGCTCATGGGAAAAATCTTGCAGAAGCAGTCGGATCAGCCAAAGAA
TACTTACACTTAAGTTTGGAAGATGATATTCTCACGGGACCAACACAACACCTAAACCACTTCCCAGATCCAACTCATTA
A

Upstream 100 bases:

>100_bases
TCGAGCCGATTTACAAAAAAAAATCTTACAAACAGAAGGTGTTTCTTTGATTTAGGCAGTGATCAATTGGATTTTGACAA
GTACGGATGGTTTCCATTAT

Downstream 100 bases:

>100_bases
TCAAAGAACTAGTTCACAAAACCGATCAAACAAAGAAGAACTCAATATTCAATATTGAGTTCTTTGATTTTTTTATCCAA
TGTGTTACGATTGATTCCCA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKKDFPITLTIAGSDSGGGAGVQADLKTFSSLATFGTTVFTCLTAQNPDGVTGIYEISPDFVSSQLQAVSSYFPIKSAKT
GMLYSKDIIKSVASFFYDNPDIQLVLDPVMVATSGAKLLKDDAIQSLIEDLIPLAKIITPNLDEASLLLGENIHQYDQLV
PMAKKLFEKYNVPILLKGGHLPNASVATDVLFDGISIFEYSKPYLKEKHTHGTGCTYSAAITAFLAHGKNLAEAVGSAKE
YLHLSLEDDILTGPTQHLNHFPDPTH

Sequences:

>Translated_266_residues
MKKDFPITLTIAGSDSGGGAGVQADLKTFSSLATFGTTVFTCLTAQNPDGVTGIYEISPDFVSSQLQAVSSYFPIKSAKT
GMLYSKDIIKSVASFFYDNPDIQLVLDPVMVATSGAKLLKDDAIQSLIEDLIPLAKIITPNLDEASLLLGENIHQYDQLV
PMAKKLFEKYNVPILLKGGHLPNASVATDVLFDGISIFEYSKPYLKEKHTHGTGCTYSAAITAFLAHGKNLAEAVGSAKE
YLHLSLEDDILTGPTQHLNHFPDPTH
>Mature_266_residues
MKKDFPITLTIAGSDSGGGAGVQADLKTFSSLATFGTTVFTCLTAQNPDGVTGIYEISPDFVSSQLQAVSSYFPIKSAKT
GMLYSKDIIKSVASFFYDNPDIQLVLDPVMVATSGAKLLKDDAIQSLIEDLIPLAKIITPNLDEASLLLGENIHQYDQLV
PMAKKLFEKYNVPILLKGGHLPNASVATDVLFDGISIFEYSKPYLKEKHTHGTGCTYSAAITAFLAHGKNLAEAVGSAKE
YLHLSLEDDILTGPTQHLNHFPDPTH

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=260, Percent_Identity=40.3846153846154, Blast_Score=177, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6325378, Length=276, Percent_Identity=33.695652173913, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324997, Length=253, Percent_Identity=35.9683794466403, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324517, Length=253, Percent_Identity=34.7826086956522, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28667; Mature: 28667

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDFPITLTIAGSDSGGGAGVQADLKTFSSLATFGTTVFTCLTAQNPDGVTGIYEISPD
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEECCHH
FVSSQLQAVSSYFPIKSAKTGMLYSKDIIKSVASFFYDNPDIQLVLDPVMVATSGAKLLK
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCHHHHH
DDAIQSLIEDLIPLAKIITPNLDEASLLLGENIHQYDQLVPMAKKLFEKYNVPILLKGGH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCC
LPNASVATDVLFDGISIFEYSKPYLKEKHTHGTGCTYSAAITAFLAHGKNLAEAVGSAKE
CCCCHHHHHHHHCCHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCHHH
YLHLSLEDDILTGPTQHLNHFPDPTH
HEEEEECCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MKKDFPITLTIAGSDSGGGAGVQADLKTFSSLATFGTTVFTCLTAQNPDGVTGIYEISPD
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEECCHH
FVSSQLQAVSSYFPIKSAKTGMLYSKDIIKSVASFFYDNPDIQLVLDPVMVATSGAKLLK
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCHHHHH
DDAIQSLIEDLIPLAKIITPNLDEASLLLGENIHQYDQLVPMAKKLFEKYNVPILLKGGH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCC
LPNASVATDVLFDGISIFEYSKPYLKEKHTHGTGCTYSAAITAFLAHGKNLAEAVGSAKE
CCCCHHHHHHHHCCHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCHHH
YLHLSLEDDILTGPTQHLNHFPDPTH
HEEEEECCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA