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The map label for this gene is capB [H]

Identifier: 183219912

GI number: 183219912

Start: 487694

End: 488890

Strand: Reverse

Name: capB [H]

Synonym: LEPBI_I0494

Alternate gene names: 183219912

Gene position: 488890-487694 (Counterclockwise)

Preceding gene: 183219914

Following gene: 183219911

Centisome position: 13.58

GC content: 37.51

Gene sequence:

>1197_bases
ATGAAACCAAATGCCACTTTATTTTTTCTCATCATTTTGGTTTTGATCCTTTATTACACCATCGAAGTGATTTTACACAA
ACGCACGTTAAAAAAATTCAAACATAGAATCCATGTCAATGGGACAAGAGGAAAATCGAGTGTAACAAGGTTAATACGAG
CAGGACTTGCCTCTTCTGGAAGTTTGGTTTTTGCAAAAACAACTGGAACTTTGGCTCGCATGATTTTTCCTGATGGATCT
GAAAAATCAATCTCTCGATTTGGAAAACCAACTATCTTAGAACAAATCAAAATCCTAAAAAAGGCAAATGAATCTGGGGC
AAGCATTGTGATTTTGGAATGTATGGCCTTGGAACCGCGTTACCAATGGGCAAGTGAGGGCCAAATTCTACAATCTGATA
TCGGAGTCATCACAAACATAAGGGAAGACCATCTGGAAGTGATGGGACCAAATCTAATTGATGTCGCCAAAACATTGTTA
TCTGCATGTCCCGTCAATGGGACTTTGGTTGTTGGACCAAGTGAATTTGAGGATAAAATCGAAAATGTATGTTTGGATCG
TAAAACAAAATCAATCCTTATCAAAGAAGCTGAGATTGAATCCATCACTGATGAGGAAATTTTAAAGTTCCCGTATTGGG
AACACAAAGAAAATGTATATCTTGCTCTCAAAGTTTGTTCCTTACTCGGAGTCGAACGAAACAAAGCATTAGAGGCAATG
TGGAAAGTCACACCGGATCCGGGAGCTCTTTCGGTTTTACCCATTCATTTTTTTGGTAAGGATTTCATATACGTGAATGC
AATGGCGGCAAATGACCCAAGTAGTACAAAATTCATTTGGACATCCACACTCAATCGATACCCTCAGATTCAAAAACGAT
TCATTTTATTCCATACTCGCGAGGATCGACCAGAACGAACCAAACAATTGACGAAAGAAATTGCCAATTGGGGTGATTAT
GATGCCGTGATTTTAATTGGTACCTCTACATCATTAGCTTTCCAATATTTAAAATCCTTCTCGCAAGCAGATGTTCCAAT
TTATCTTTGGGAACACTTAAGTTTAGACGGAATCTTTGAATCTTTACTTTCTATCCTTCCGAAACAATCTTTGGTATTTG
GTATAGGGAATATTGTTGGACTTGGAATGGAATTGTCTTTATATCTAAAAAATAGGTCAGAACAAACGTATGAATGA

Upstream 100 bases:

>100_bases
TGTAACGTATGCTCATAGAAAACTACGTGAATCTTCTCAAATTTACGTTACAATCAAATGACATTCCTTTGAAGGAAATG
TATCATCTGGAAATTTTCAA

Downstream 100 bases:

>100_bases
AATTCTCCCGTTGTCGATTGGACTGAGTTTAGTCATCAGTTTGGTATTTTCTGAATTGTTTGGTATTTTAGGTACAGGTC
TCGTTGTGCCAGGTTATCTT

Product: polyglutamyl synthetase CapB family protein

Products: NA

Alternate protein names: Gamma-polyglutamic acid synthase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKPNATLFFLIILVLILYYTIEVILHKRTLKKFKHRIHVNGTRGKSSVTRLIRAGLASSGSLVFAKTTGTLARMIFPDGS
EKSISRFGKPTILEQIKILKKANESGASIVILECMALEPRYQWASEGQILQSDIGVITNIREDHLEVMGPNLIDVAKTLL
SACPVNGTLVVGPSEFEDKIENVCLDRKTKSILIKEAEIESITDEEILKFPYWEHKENVYLALKVCSLLGVERNKALEAM
WKVTPDPGALSVLPIHFFGKDFIYVNAMAANDPSSTKFIWTSTLNRYPQIQKRFILFHTREDRPERTKQLTKEIANWGDY
DAVILIGTSTSLAFQYLKSFSQADVPIYLWEHLSLDGIFESLLSILPKQSLVFGIGNIVGLGMELSLYLKNRSEQTYE

Sequences:

>Translated_398_residues
MKPNATLFFLIILVLILYYTIEVILHKRTLKKFKHRIHVNGTRGKSSVTRLIRAGLASSGSLVFAKTTGTLARMIFPDGS
EKSISRFGKPTILEQIKILKKANESGASIVILECMALEPRYQWASEGQILQSDIGVITNIREDHLEVMGPNLIDVAKTLL
SACPVNGTLVVGPSEFEDKIENVCLDRKTKSILIKEAEIESITDEEILKFPYWEHKENVYLALKVCSLLGVERNKALEAM
WKVTPDPGALSVLPIHFFGKDFIYVNAMAANDPSSTKFIWTSTLNRYPQIQKRFILFHTREDRPERTKQLTKEIANWGDY
DAVILIGTSTSLAFQYLKSFSQADVPIYLWEHLSLDGIFESLLSILPKQSLVFGIGNIVGLGMELSLYLKNRSEQTYE
>Mature_398_residues
MKPNATLFFLIILVLILYYTIEVILHKRTLKKFKHRIHVNGTRGKSSVTRLIRAGLASSGSLVFAKTTGTLARMIFPDGS
EKSISRFGKPTILEQIKILKKANESGASIVILECMALEPRYQWASEGQILQSDIGVITNIREDHLEVMGPNLIDVAKTLL
SACPVNGTLVVGPSEFEDKIENVCLDRKTKSILIKEAEIESITDEEILKFPYWEHKENVYLALKVCSLLGVERNKALEAM
WKVTPDPGALSVLPIHFFGKDFIYVNAMAANDPSSTKFIWTSTLNRYPQIQKRFILFHTREDRPERTKQLTKEIANWGDY
DAVILIGTSTSLAFQYLKSFSQADVPIYLWEHLSLDGIFESLLSILPKQSLVFGIGNIVGLGMELSLYLKNRSEQTYE

Specific function: Catalyzes the biosynthesis of PGA (gamma-polyglutamic acid) from L-glutamate. Both the 44-kDa and the 33-kDa forms are required for PGA synthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008337
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: NA

Molecular weight: Translated: 44902; Mature: 44902

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNATLFFLIILVLILYYTIEVILHKRTLKKFKHRIHVNGTRGKSSVTRLIRAGLASSG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCCCCC
SLVFAKTTGTLARMIFPDGSEKSISRFGKPTILEQIKILKKANESGASIVILECMALEPR
CEEEEECCCCEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCC
YQWASEGQILQSDIGVITNIREDHLEVMGPNLIDVAKTLLSACPVNGTLVVGPSEFEDKI
CCCCCCCCEEHHCCHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHH
ENVCLDRKTKSILIKEAEIESITDEEILKFPYWEHKENVYLALKVCSLLGVERNKALEAM
HHHHHCCCCHHEEEEEHHHHCCCHHHHHHCCCCCCCCCEEEHEEHHHHHCCCHHHHHHHH
WKVTPDPGALSVLPIHFFGKDFIYVNAMAANDPSSTKFIWTSTLNRYPQIQKRFILFHTR
HEECCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCEEEEEHHHHCCHHHHEEEEEEECC
EDRPERTKQLTKEIANWGDYDAVILIGTSTSLAFQYLKSFSQADVPIYLWEHLSLDGIFE
CCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEHHCCHHHHHH
SLLSILPKQSLVFGIGNIVGLGMELSLYLKNRSEQTYE
HHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MKPNATLFFLIILVLILYYTIEVILHKRTLKKFKHRIHVNGTRGKSSVTRLIRAGLASSG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCCCCC
SLVFAKTTGTLARMIFPDGSEKSISRFGKPTILEQIKILKKANESGASIVILECMALEPR
CEEEEECCCCEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCC
YQWASEGQILQSDIGVITNIREDHLEVMGPNLIDVAKTLLSACPVNGTLVVGPSEFEDKI
CCCCCCCCEEHHCCHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHH
ENVCLDRKTKSILIKEAEIESITDEEILKFPYWEHKENVYLALKVCSLLGVERNKALEAM
HHHHHCCCCHHEEEEEHHHHCCCHHHHHHCCCCCCCCCEEEHEEHHHHHCCCHHHHHHHH
WKVTPDPGALSVLPIHFFGKDFIYVNAMAANDPSSTKFIWTSTLNRYPQIQKRFILFHTR
HEECCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCEEEEEHHHHCCHHHHEEEEEEECC
EDRPERTKQLTKEIANWGDYDAVILIGTSTSLAFQYLKSFSQADVPIYLWEHLSLDGIFE
CCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEHHCCHHHHHH
SLLSILPKQSLVFGIGNIVGLGMELSLYLKNRSEQTYE
HHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353933; 10486244; 11751809; 9384377 [H]