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The map label for this gene is gacS [H]

Identifier: 183219905

GI number: 183219905

Start: 480441

End: 482231

Strand: Reverse

Name: gacS [H]

Synonym: LEPBI_I0487

Alternate gene names: 183219905

Gene position: 482231-480441 (Counterclockwise)

Preceding gene: 183219906

Following gene: 183219904

Centisome position: 13.4

GC content: 36.07

Gene sequence:

>1791_bases
TTGTTTCCCTATCTACTCACCAATTTCATTTTATTTATTTTTGTATCCATAGCCTTTGCTTTTTACACTGCTAGTGAACG
AAACATTGATGCGGCGGAGGAAAGCCGGCATCGGTCGTTACAAATTGCCAATGAACTCCGCCAGTCATCAGACCAACTAA
CAAACCTTGTCCGTTTGTATGCAATTCAAAAAGAACCAAAATTCAAAGCTTACTTCCAACGCATTTTAGAGATCCGAAAT
GGTGTTAGGCCACGACCTAATGAGTATGACTATGCTTATTGGGATTTGGTCATTGCCGATCAATTACCTCCACCTTCCGA
AGAGGGGGAAAAGATCAGCATTTTGGATGCCATGAAACAAGCTGGTTTTTTAGAGTCAGATTTTTTATTACTATCCTTAT
CTAAAGAAAAATCAGACCAACTCACCAAAATCGAATTTGAATCCATGTCGATGATCGACAGGGAATTAAAAACTGGAAAA
TCCAATCCCAAAGCAGTAGCAATTTTATTTGATGATCAGTATCTGAAATTCAAAGCAGAGATTATGAAACCAATCAATGA
TTTATACCACCAATTGGATGACCGGACAACTCAGGCAATATTGGATGCAAAAGAAAAAGTTTTTTTCCTACGAACAGTTC
TTATCTTATCTGGAATTGTTTTTGGAATTAGTTTGTATTTCACACATCGATCGTTGGTGTCCATTATGGGAGGGAGTGTC
GACGAAGTCTTTCGACTCATTTCTTTACTTGGTGAAGGAAAATTTACAGGTGATATCCAAATCTTAAATGATAACAAAAC
AATCTTAAATAGTTTAAATACGACACAAAAAAGGTTACAGGAGTTATACGAAAAGACAGAAATGGCAAGTCGTGCCAAAT
CTGAATTTTTAGCATCGATGAGTCATGAGATACGAACTCCTCTGAACGGAGTGATTGGAATCACACAGATTTTATTCGAA
ACAGATTTAGATGCAGAACAAAAGTCCTATTTAAAAACGATCGCTGATGCAGGAAAAGCACTCTTAAATATTCTAAACGA
TATTCTAGATTTCTCAAAAATCGATGCGGGAAAATTAACAATTCAAAAAACTCCATTTCACTTAGCAAATTTAGTCAGGG
AAATTTTTGATTTATTTGCCATCGAAGCAAATACCAAACAATTAGAATTTAAATTCGAAATTAATCCCGATGTGCCTGAG
GTGATTGTTTCAGATCCAAGTCGAATTCGACAAATTTTATTCAACTTAATTGGAAATGCGATTAAATTTACGGAAACTGG
ATTCGTGATTCTTTCGATCAAAAAAGAAGAACATTCGATCTTATTTCAAATCAAAGATTCAGGAATTGGAATTTCCTATG
AAAAACTCACTTCTTTATTCCAAAAATTTTCGCAAGTTGATACTTCCACCTCACGTAAGTATGGTGGAACTGGGCTCGGC
CTTGCCATTTCCGAAAGATTGGTTGGTCTTTTAGAGGGAAAAATTGGTGTGGAAAGTGTGAAAGGAGTAGGAAGTACATT
TTGGTGTTCGTTTCCACTTAGCATAGAGGAAGAAACCAATTCCTACCAAATTGAAATTTCGTCGCCTGTATCCAAACAAA
TTCCAATTTCAGATTCATTGGAAATGAACAAAAAACCAAATGTGGTTCCATTTGATGGGAAACGAGTTTTAGTCGTAGAA
GACAATGTATTAAACCAAAAAGTGATTGGTGGGTTACTCAAAAAACAAAAGATCCAATCCGAATCAAACTCCTTCCATCA
TCATTGCAGTGACAGCCCATGTCTTAAATGA

Upstream 100 bases:

>100_bases
TTCCCAGATCAACTCTCCAAACGAATATAATCCTTTACTCCTTTTTCATTTCCTGTAAAGTATCGCCATGTTGGATAGAA
CTTGGAAACCAAAAGGAAGA

Downstream 100 bases:

>100_bases
ACACAAAGAAAGATGTTTGGAAGTGGGTATGGATGGATTCGTAAGCAAACCATTTTACATTGAAAACTTGTTACAAACGT
ATCGAGAAATTTTATACAAA

Product: putative two-component sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MFPYLLTNFILFIFVSIAFAFYTASERNIDAAEESRHRSLQIANELRQSSDQLTNLVRLYAIQKEPKFKAYFQRILEIRN
GVRPRPNEYDYAYWDLVIADQLPPPSEEGEKISILDAMKQAGFLESDFLLLSLSKEKSDQLTKIEFESMSMIDRELKTGK
SNPKAVAILFDDQYLKFKAEIMKPINDLYHQLDDRTTQAILDAKEKVFFLRTVLILSGIVFGISLYFTHRSLVSIMGGSV
DEVFRLISLLGEGKFTGDIQILNDNKTILNSLNTTQKRLQELYEKTEMASRAKSEFLASMSHEIRTPLNGVIGITQILFE
TDLDAEQKSYLKTIADAGKALLNILNDILDFSKIDAGKLTIQKTPFHLANLVREIFDLFAIEANTKQLEFKFEINPDVPE
VIVSDPSRIRQILFNLIGNAIKFTETGFVILSIKKEEHSILFQIKDSGIGISYEKLTSLFQKFSQVDTSTSRKYGGTGLG
LAISERLVGLLEGKIGVESVKGVGSTFWCSFPLSIEEETNSYQIEISSPVSKQIPISDSLEMNKKPNVVPFDGKRVLVVE
DNVLNQKVIGGLLKKQKIQSESNSFHHHCSDSPCLK

Sequences:

>Translated_596_residues
MFPYLLTNFILFIFVSIAFAFYTASERNIDAAEESRHRSLQIANELRQSSDQLTNLVRLYAIQKEPKFKAYFQRILEIRN
GVRPRPNEYDYAYWDLVIADQLPPPSEEGEKISILDAMKQAGFLESDFLLLSLSKEKSDQLTKIEFESMSMIDRELKTGK
SNPKAVAILFDDQYLKFKAEIMKPINDLYHQLDDRTTQAILDAKEKVFFLRTVLILSGIVFGISLYFTHRSLVSIMGGSV
DEVFRLISLLGEGKFTGDIQILNDNKTILNSLNTTQKRLQELYEKTEMASRAKSEFLASMSHEIRTPLNGVIGITQILFE
TDLDAEQKSYLKTIADAGKALLNILNDILDFSKIDAGKLTIQKTPFHLANLVREIFDLFAIEANTKQLEFKFEINPDVPE
VIVSDPSRIRQILFNLIGNAIKFTETGFVILSIKKEEHSILFQIKDSGIGISYEKLTSLFQKFSQVDTSTSRKYGGTGLG
LAISERLVGLLEGKIGVESVKGVGSTFWCSFPLSIEEETNSYQIEISSPVSKQIPISDSLEMNKKPNVVPFDGKRVLVVE
DNVLNQKVIGGLLKKQKIQSESNSFHHHCSDSPCLK
>Mature_596_residues
MFPYLLTNFILFIFVSIAFAFYTASERNIDAAEESRHRSLQIANELRQSSDQLTNLVRLYAIQKEPKFKAYFQRILEIRN
GVRPRPNEYDYAYWDLVIADQLPPPSEEGEKISILDAMKQAGFLESDFLLLSLSKEKSDQLTKIEFESMSMIDRELKTGK
SNPKAVAILFDDQYLKFKAEIMKPINDLYHQLDDRTTQAILDAKEKVFFLRTVLILSGIVFGISLYFTHRSLVSIMGGSV
DEVFRLISLLGEGKFTGDIQILNDNKTILNSLNTTQKRLQELYEKTEMASRAKSEFLASMSHEIRTPLNGVIGITQILFE
TDLDAEQKSYLKTIADAGKALLNILNDILDFSKIDAGKLTIQKTPFHLANLVREIFDLFAIEANTKQLEFKFEINPDVPE
VIVSDPSRIRQILFNLIGNAIKFTETGFVILSIKKEEHSILFQIKDSGIGISYEKLTSLFQKFSQVDTSTSRKYGGTGLG
LAISERLVGLLEGKIGVESVKGVGSTFWCSFPLSIEEETNSYQIEISSPVSKQIPISDSLEMNKKPNVVPFDGKRVLVVE
DNVLNQKVIGGLLKKQKIQSESNSFHHHCSDSPCLK

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=352, Percent_Identity=38.3522727272727, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87081816, Length=368, Percent_Identity=34.2391304347826, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI48994928, Length=305, Percent_Identity=37.0491803278689, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI145693157, Length=238, Percent_Identity=38.655462184874, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788713, Length=251, Percent_Identity=33.4661354581673, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1786783, Length=333, Percent_Identity=27.6276276276276, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=29.4642857142857, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790436, Length=233, Percent_Identity=28.3261802575107, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788393, Length=250, Percent_Identity=28.4, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789808, Length=233, Percent_Identity=28.755364806867, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788549, Length=265, Percent_Identity=25.6603773584906, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87082128, Length=316, Percent_Identity=22.1518987341772, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790346, Length=212, Percent_Identity=27.3584905660377, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=302, Percent_Identity=24.8344370860927, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786912, Length=250, Percent_Identity=24.8, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790300, Length=241, Percent_Identity=26.1410788381743, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322044, Length=135, Percent_Identity=37.037037037037, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67369; Mature: 67369

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50042 CNMP_BINDING_3 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPYLLTNFILFIFVSIAFAFYTASERNIDAAEESRHRSLQIANELRQSSDQLTNLVRLY
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIQKEPKFKAYFQRILEIRNGVRPRPNEYDYAYWDLVIADQLPPPSEEGEKISILDAMKQ
HHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEHHHHHHHH
AGFLESDFLLLSLSKEKSDQLTKIEFESMSMIDRELKTGKSNPKAVAILFDDQYLKFKAE
CCCCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
IMKPINDLYHQLDDRTTQAILDAKEKVFFLRTVLILSGIVFGISLYFTHRSLVSIMGGSV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DEVFRLISLLGEGKFTGDIQILNDNKTILNSLNTTQKRLQELYEKTEMASRAKSEFLASM
HHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHEIRTPLNGVIGITQILFETDLDAEQKSYLKTIADAGKALLNILNDILDFSKIDAGKLT
HHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IQKTPFHLANLVREIFDLFAIEANTKQLEFKFEINPDVPEVIVSDPSRIRQILFNLIGNA
EEECCHHHHHHHHHHHHHHEECCCCEEEEEEEEECCCCCHHEECCHHHHHHHHHHHHCCC
IKFTETGFVILSIKKEEHSILFQIKDSGIGISYEKLTSLFQKFSQVDTSTSRKYGGTGLG
EEEECCCEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCH
LAISERLVGLLEGKIGVESVKGVGSTFWCSFPLSIEEETNSYQIEISSPVSKQIPISDSL
HHHHHHHHHHHHCCCCHHHHCCCCCEEEEECCCEEECCCCCEEEEECCCCCCCCCCCCCC
EMNKKPNVVPFDGKRVLVVEDNVLNQKVIGGLLKKQKIQSESNSFHHHCSDSPCLK
CCCCCCCEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFPYLLTNFILFIFVSIAFAFYTASERNIDAAEESRHRSLQIANELRQSSDQLTNLVRLY
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIQKEPKFKAYFQRILEIRNGVRPRPNEYDYAYWDLVIADQLPPPSEEGEKISILDAMKQ
HHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEHHHHHHHH
AGFLESDFLLLSLSKEKSDQLTKIEFESMSMIDRELKTGKSNPKAVAILFDDQYLKFKAE
CCCCCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
IMKPINDLYHQLDDRTTQAILDAKEKVFFLRTVLILSGIVFGISLYFTHRSLVSIMGGSV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DEVFRLISLLGEGKFTGDIQILNDNKTILNSLNTTQKRLQELYEKTEMASRAKSEFLASM
HHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHEIRTPLNGVIGITQILFETDLDAEQKSYLKTIADAGKALLNILNDILDFSKIDAGKLT
HHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IQKTPFHLANLVREIFDLFAIEANTKQLEFKFEINPDVPEVIVSDPSRIRQILFNLIGNA
EEECCHHHHHHHHHHHHHHEECCCCEEEEEEEEECCCCCHHEECCHHHHHHHHHHHHCCC
IKFTETGFVILSIKKEEHSILFQIKDSGIGISYEKLTSLFQKFSQVDTSTSRKYGGTGLG
EEEECCCEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCH
LAISERLVGLLEGKIGVESVKGVGSTFWCSFPLSIEEETNSYQIEISSPVSKQIPISDSL
HHHHHHHHHHHHCCCCHHHHCCCCCEEEEECCCEEECCCCCEEEEECCCCCCCCCCCCCC
EMNKKPNVVPFDGKRVLVVEDNVLNQKVIGGLLKKQKIQSESNSFHHHCSDSPCLK
CCCCCCCEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]